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Energy TransferFluorescence Resonance Energy TransferEnergy MetabolismSpectrometry, FluorescenceLinear Energy TransferEnergy IntakeFluorescent DyesGene Transfer TechniquesFluorescenceBioluminescence Resonance Energy Transfer TechniquesLuminescent ProteinsKineticsModels, MolecularGene Transfer, HorizontalEmbryo TransferFluorescence PolarizationProtein BindingTerbiumProtein ConformationLight-Harvesting Protein ComplexesBiosensing TechniquesThermodynamicsModels, ChemicalGreen Fluorescent ProteinsNaphthalenesulfonatesLuminescent MeasurementsPhotobleachingRhodaminesMolecular Sequence DataBinding SitesMicroscopy, FluorescenceTryptophanPhotonsChlorophyllBacteriochlorophyllsDimerizationTransfer (Psychology)Protein Structure, TertiaryTime FactorsBacterial ProteinsTemperatureLuminescenceModels, BiologicalCarbocyaninesElectron TransportCell MembraneLightPhotosynthetic Reaction Center Complex ProteinsRNA, TransferRecombinant Fusion ProteinsBiophysicsLiposomesSpectrophotometryCell LineBiophysical PhenomenaDansyl CompoundsAmino Acid SequencePhycocyaninEscherichia coliFluoresceinsProtein MultimerizationPhotosystem I Protein ComplexMutationPhycobilisomesLuciferases, RenillaHeavy IonsDNAAdoptive TransferElectronsQuantum TheoryFluoresceinCarotenoidsMathematicsSpectrum AnalysisBase SequenceSolar EnergyPhotosynthesisConservation of Energy ResourcesPhospholipid Transfer ProteinsPatient TransferMicroscopy, ConfocalPhotochemistryFluorescein-5-isothiocyanateNucleic Acid ConformationPhotosystem II Protein ComplexAdenosine TriphosphateGenetic VectorsHydrogen-Ion ConcentrationMolecular ConformationAlpha ParticlesPhycoerythrinModels, TheoreticalProtein Structure, QuaternaryOxidation-ReductionQuantum DotsTransfectionLipid BilayersComputer SimulationTransfer FactorLigands
FluorescenceGreen fluoresFRETProteinsDyesMoleculesAcceptorEmissionResonanceProteinPhotonsDETECTIONRatiometricLonger wavelengthFluorophoreExcitation energyNanoparticlesSpectraSpectralPolymerReliesVivoProbeBeaconsJellyfishLightIntensityPhotonTagsYellow-greenTightlyCaseViewApplicationsLongSystemLowerHighCompactColor
Fluorescence16
  • The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. (wikipedia.org)
  • Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37 °C (99 °F). The first reported crystal structure of a GFP was that of the S65T mutant by the Remington group in Science in 1996. (wikipedia.org)
  • Fluorescence is an essentially background-free signal, to the point that single fluorescent molecules can be observed. (ibs.fr)
  • mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins. (nature.com)
  • The decay times of this fluorescence is of the order of nanoseconds since the duration of the light depends on the lifetime of the excited states of the fluorescent material, in this case anthracene or stilbene. (newworldencyclopedia.org)
  • The common fluorescent tube relies on fluorescence. (newworldencyclopedia.org)
  • Biological molecules can be tagged with a fluorescent chemical group (fluorophore) by a simple chemical reaction , and the fluorescence of the tag enables sensitive and quantitative detection of the molecule. (newworldencyclopedia.org)
  • Fluorescence is a physical phenomenon in which the absorption of light photons triggers in the molecule of fluorescent compound an emission of photons with longer wavelength (but lower energy). (cas.cz)
  • Due to the energy transfer, excitation by a light of appropriate wavelength triggers a fluorescence emission from chlorophyll-a molecule. (cas.cz)
  • Dr. Blanchard adapted an imaging technique that uses fluorescence to measure distance on molecular scale -- single-molecule fluorescence resonance energy transfer (smFRET) imaging -- to study viral particles. (sciencedaily.com)
  • It can be used in time-resolved fluorescence resonance energy-transfer (TR-FRET) cereblon binding assays for PROTAC ® Degrader development. (tocris.com)
  • All dyes are fully characterized and can be used in time resolved spectroscopy, FRET (Fluorescence Resonance Energy Transfer) Real-Time qPCR assays applications as well as single- molecule detection. (eurogentec.com)
  • Structural basis for bathochromic shift of fluorescence in far-red fluorescent proteins eqFP650 and eqFP670. (evrogen.com)
  • Fluorescence resonance energy transfer (FRET) prohibits emission of the fluorophore while the oligo probe is intact. (neb.com)
  • SmMIET is based on the distance-dependent quenching of the fluorescence emission of fluorescent molecules by metallic or semi-metallic thin films, and it requires labeling of the peptide/protein of interest by only one dye, in contrast to single-molecule Förster Resonance Energy Transfer, which requires labeling with a donor and an acceptor dye label. (uni-goettingen.de)
  • developed a fluorescence resonance energy transfer PCR assay that targets the B. anthracis chromosomally located rpoB gene. (cdc.gov)
Green fluores8
  • To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
  • Scientists Roger Y. Tsien, Osamu Shimomura, and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. (wikipedia.org)
  • For FPs, one often compares the MB to that of enhanced Green Fluorescent Protein (EGFP), which is about 30000 M -1 .cm -1 . (ibs.fr)
  • We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum . (nature.com)
  • Green fluorescent protein has revolutionized cell labeling and molecular tagging, yet the driving force and mechanism for its spontaneous fluorophore synthesis are not established. (proteopedia.org)
  • Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures. (proteopedia.org)
  • Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED. Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures. (proteopedia.org)
  • We employ Förster Resonance Energy Transfer (FRET) between identical (single color) green fluorescent protein (GFP) tags linked to α-synuclein. (lu.se)
FRET4
  • Fluorescent Resonance Energy Transfer [FRET] is the transfer of excitation energy from a donor fluorescent molecule to an acceptor fluorescent molecule. (duke.edu)
  • Joo will develop a new ultrahigh resolution concept in single-molecule FRET (Forster resonance energy transfer) detection, named FRET X. FRET is the transfer of energy between fluorescent molecules, which occurs when they are placed within several nanometers. (tudelft.nl)
  • The efficiency of this transfer is sensitive to very small distance changes, making FRET an outstanding ruler for probing biological systems. (tudelft.nl)
  • Herein we report a FRET-based, 1O2-sensitive aqueous suspension of conjugated polymer nanoparticles (CPNs) comprising the energy donating host polymer poly[(9,9-dioctyl-2,7-divinylene-fluorenylene(-alt-co-(2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylene)] doped with an energy acceptor, the 1O2-sensitive thienoacene 5,12-bis(4-methoxyphenyl)-2-butyltetraceno[2,3-b]thiophene. (tufts.edu)
Proteins7
  • Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long. (wikipedia.org)
  • This molecule carries energy all through the body, and most cells and proteins require ATP to perform their various roles. (elifesciences.org)
  • Förster Resonance Energy Transfer-Paired Hydrogel Forming Silk-Elastin-Like Recombinamers by Recombinant Conjugation of Fluorescent Proteins. (evrogen.com)
  • Cell-based and in vivo spectral analysis of fluorescent proteins for multiphoton microscopy. (evrogen.com)
  • Near-infrared fluorescent proteins. (evrogen.com)
  • Fluorophores for STORM and SMLM include synthetic dyes, fluorescent proteins (FPs), and even Quantum Dots (QDots). (microscopyu.com)
  • The core experimental aim of the project is to develop a new single-molecule spectroscopic method, dynamic single-molecule Metal Induced Energy Transfer (or dynamic smMIET), which will be suited to study the conformational dynamics of intrinsically disordered peptides (sequences of FG-repeat proteins from the nuclear pore), and of small folding protein motifs, in particular helix-turn-helix (HTH) and WW-domain motifs. (uni-goettingen.de)
Dyes7
  • Although BD APC-H7 is engineered to minimize spillover to the APC channel and is more stable and less affected by light, temperature, and formaldehyde-based fixatives, compared to other APC-cyanine tandem dyes, it is still good practice to minimize as much as possible, any light, temperature and fixative exposure when working with all fluorescent conjugates. (bdbiosciences.com)
  • Furthermore, the fixed spectral characteristics of interference filters do not allow optimization for different fluorophore combinations, nor for adaptation to new fluorescent dyes, limiting the versatility of both the excitation and detection functions of the microscope. (olympusconfocal.com)
  • View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes. (tocris.com)
  • ATTO-TEC dyes can be used as fluorescent labels for a large variety of biomolecules including oligonucleotides. (eurogentec.com)
  • ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B is the commercial alternative to NED. (eurogentec.com)
  • ATTO 565, ATTO 590 and ATTO 594 are fluorescent labels belonging to the class of Rhodamine dyes. (eurogentec.com)
  • Many antifreeze products also contain yellow-green fluorescent dyes and a bitter taste to reduce the chances of accidental ingestion. (cdc.gov)
Molecules1
  • His group developed fluorescent molecules (fluorophores) -- which he dubs "beacons" -- and the team inserted them into the virus's outer covering, known as the envelope. (sciencedaily.com)
Acceptor1
Emission2
  • Analyte binding to the receptor causes attenuation of the HOMO-LUMO gap at the receptor terminus leading to the ratiometric change in fluorescent emission. (gov.np)
  • The resulting fluorescent emission maximum is approximately 767 nm. (bdbiosciences.com)
Resonance2
Protein5
  • As shown on the figure (so called Jablonski diagram), when a molecule (e.g. a fluorescent protein) in the electronic ground state absorbs a photon of suitable wavelength, it accesses the first electronically excited state. (ibs.fr)
  • made several potential sensors by attaching various fluorescent tags to different locations on a protein that binds ATP. (elifesciences.org)
  • The respective part of photosynthetic apparatus of all photoautotrophic organisms, which is responsible of capturing light, is created by pigment-protein complexes containing chlorophyll-a and other accessorial pigments ( chlorophyll-b, phycocyanine, phycoerythrine, fucoxantine, peridinine etc.) which help to absorb the energy of light and pass it to chlorophyll-a. (cas.cz)
  • Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. (proteopedia.org)
  • An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging. (evrogen.com)
Photons3
  • Molecular brightness (MB) is defined as the product of the QY and Extinction coefficient, and therefore represents capacity of a fluorescent molecule to emit photons under a given intensity and colour of excitation light. (ibs.fr)
  • A two-site ELISA for oligomers was developed using 19.3 coupled to a sensitive, bead-based fluorescent platform able to detect single photons of emitted light. (jneurosci.org)
  • The energy difference between the absorbed and emitted photons ends up as molecular vibrations or heat . (newworldencyclopedia.org)
DETECTION2
  • Molecular devices possessing -electron conjugated scaffold demonstrate unique capabilities as fluorescent chemosensors for the trace detection of analytes in a variety of environments. (gov.np)
  • Chapter 3 describes that while fluorescent probes for the detection of singlet oxygen (1O2) have been an active area of research, most such probes rely upon change in intensity of a single band. (tufts.edu)
Ratiometric4
  • Towards this goal, we designed surface-immobilized monodispersed oligo(p-phenylene ethynylene)s (OPEs) as a general basis for thin-film ratiometric fluorescent chemosensors. (gov.np)
  • The thin-film sensors for L-cysteine, pH and Zn2+ were prepared and studied in detail to uncover a number of unusual properties, thus demonstrating the potential of this platform as a universal foundation for designing a broad range of fluorescent ratiometric thin-film chemosensors. (gov.np)
  • Chapter 4 describes effects of nanoparticle composition on energy transfer and ratiometric fluorescent response to 1O2 of a series of dye-doped CPNs. (tufts.edu)
  • The doping levels of acenes in the CP matrix have important implications for both the extent of energy transfer and the subsequent rates of ratiometric response. (tufts.edu)
Longer wavelength1
  • When excited by light from a red laser, the APC fluorochrome can transfer energy to the cyanine dye, which then emits at a longer wavelength. (bdbiosciences.com)
Fluorophore1
  • A second fluorophore is used to label an exatracelluar surface and undergoes energy transfer with the membrane bound sensor. (duke.edu)
Excitation energy1
  • The main goal of this Ph.D. dissertation was to study the possibility to control excitation energy migration in conjugated systems. (gov.np)
Nanoparticles1
  • Ulta-Bright Fluorescent Organic Nanoparticles based on Small-Molecule Ionic Isolation Lattices , Junsheng Chen, S. M. Ali Fateminia, Laura Kacenauskaite, Nicolai Bærentsen, Karen L. Martinez, Amar H. Flood, Bo W. Laursen. (lu.se)
Spectra1
  • Fluorescent lighting is very energy efficient compared to incandescent technology, but the spectra produced may cause certain colors to appear unnatural. (newworldencyclopedia.org)
Spectral2
  • ATTO 610, ATTO 611X, ATTO 620, ATTO 633, ATTO 637, ATTO 647 and ATTO 647N are fluorescent labels for the red spectral region. (eurogentec.com)
  • ATTO 725 together with ATTO 740 are fluorescent labels for the near infrared spectral region. (eurogentec.com)
Polymer1
  • One-pot fabrication of amphiphilic photoswitchable thiophene-based fluorescent polymer dots. (mpg.de)
Relies1
  • The mechanism relies on the rapid redistribution of Hydrophobic fluorescent onions. (duke.edu)
Vivo1
  • Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. (proteopedia.org)
Probe2
  • BDY FL Thalidomide is a high-affinity fluorescent probe (K d = 3.6 nM). (tocris.com)
  • Probe-based qPCR allows multiple targets to be quantified in a single reaction (multiplexing) by using a unique fluorescent dye for each amplicon-specific probe. (neb.com)
Beacons1
  • Finally, the use of this fluorescent readout was representatively demonstrated in molecular beacons. (uni-regensburg.de)
Jellyfish1
  • Remarkably, the GFP molecule folded and was fluorescent at room temperature, without the need for exogenous cofactors specific to the jellyfish. (wikipedia.org)
Light3
  • The tube is lined with a coating of a fluorescent material, called the phosphor , which absorbs the ultraviolet and re-emits visible light. (newworldencyclopedia.org)
  • Photoautotrophic organisms (cyanobacteria, algae, green plants) need energy for the production of organic compounds and therefore they employ the energy of light, which they absorb, into process called photosynthesis . (cas.cz)
  • A fluorescent tube held near the coil will light up brightly, demonstrating wireless energy transfer. (paraflows.at)
Intensity1
  • The fluorescent anion experiments have shown the expected decrease in fluorescent intensity with a decrease in transmembrane potential. (duke.edu)
Photon4
  • When you request a photon energy, you are dealing with a higher-order system that will automatically configure the monochromator and undulator correctly. (lu.se)
  • The polarization buttons on the control panel perform a simple process of changing the undulator phase and then 'wobbling' the photon energy up and down by 0.1eV to trigger the higher-order process to configure the undulator correctly for the desired photon energy. (lu.se)
  • M1 pitch is set automatically by a supervisor script (see below), but today this only works for photon energies above approximately 23eV. (lu.se)
  • Besides analyzer and scan settings, currently only manipulator position and photon energy are saved in measurement metadata. (lu.se)
Tags1
Yellow-green1
  • in that application, it is often mixed with a yellow-green fluorescent. (cdc.gov)
Tightly3
  • This one packs an interconnected network of graphene and carbon nanotubes so tightly that it stores energy comparable to some thin-film lithium batteries an area where batteries have traditionally held a large advantage. (nanotech-now.com)
  • But the materials are tightly packed in the capillary column and remain so as they're pumped out, resulting in the high volumetric energy density. (nanotech-now.com)
  • The scaled down transfer of a naturalistic behavior into a tightly controlled laboratory setting now opens the path to directly investigate the mechanisms underlying navigational memories in the behaving bee. (lu.se)
Case2
  • The product's developers, engineers and scientists at Nanyang Technological University (NTU) in Singapore, Tsinghua University in China, and Case Western Reserve University in the United States, believe the storage capacity by volume (called volumetric energy density) is the highest reported for carbon-based microscale supercapacitors to date: 6.3 microwatt hours per cubic millimeter. (nanotech-now.com)
  • Dai, a professor of macromolecular science and engineering at Case Western Reserve and a co-author of the paper, explained that most supercapacitors have high power density but low energy density, which means they can charge quickly and give a boost of power, but don't last long. (nanotech-now.com)
View1
  • It does this by examining a camera view of the fluorescent coating on the exit slit horizontal gap blades, which gives information about the horizontal beam position. (lu.se)
Applications1
  • ATTO-TEC fluorescent labels are ideal for many bio-analytical applications. (eurogentec.com)
Long1
  • Conversely, batteries have high energy density and low power density, which means they can last a long time, but don't deliver a large amount of energy quickly. (nanotech-now.com)
System1
  • Biolog's Quality Management System is certified according to DIN EN ISO 9001:2015. (biolog.de)
Lower1
  • For lower energies you will need to manage the M1 pitch by hand, and unfortunately this is also the high heatload condition where you can expect time drift of the optimal M1 pitch value. (lu.se)
High1
  • Microelectronics to electric vehicles can benefit from energy storage devices that offer high power and high energy density. (nanotech-now.com)
Compact2
  • Compact fluorescent lighting (CFL) is the same as any typical fluorescent lamp with advantages. (newworldencyclopedia.org)
  • The STIHL electric motor (EC) is extremely energy efficient, lightweight and compact. (stihl.com)
Color1
  • Some of this luminescent energy is transferred to the GFP, shifting the overall color towards green. (wikipedia.org)