• Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. (wikipedia.org)
  • A fluorescence polarization immunoassay was first described and used in the 1960s. (wikipedia.org)
  • The competitive homogenous characteristic allowed for the fluorescence polarization immunoassay to be automated much easier than other immunoassay techniques such as radioimmunoassays or enzyme-linked immunoassays. (wikipedia.org)
  • ELISA Radio immunoassay FRET Magnetic immunoassay Fluorescence Immunoscreening Lateral flow test Cloned enzyme donor immunoassay Surround optical fiber immunoassay Plate reader Nielsen K, Lin M, Gall D, Jolley M (September 2000). (wikipedia.org)
  • Cody JT, Schwarzhoff R. "Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine analogues" J Anal Toxicol . (erowid.org)
  • RePub, Erasmus University Repository: Using fluorescence polarization immunoassay for determination of erythrocyte methotrexate polyglutamates, a quick and easy test? (eur.nl)
  • Using fluorescence polarization immunoassay for determination of erythrocyte methotrexate polyglutamates, a quick and easy test? (eur.nl)
  • A fluorescence polarization immunoassay (FPIA) based on a polyclonal antibody for the determination of Sulfamethazine (SMZ) was developed. (ipmnet.ru)
  • The method is a fluorescence polarization immunoassay (FPIA) from Abbott Diagnostics. (cdc.gov)
  • Amikacin concentration was quantified by a fluorescence polarization immunoassay. (avma.org)
  • Gentamicin concentrations in synovial fluid and serum were determined for 120 hours after implantation by use of a fluorescence polarization immunoassay. (avma.org)
  • We employed the X-ray crystal structure of the PPARγ subtype complexed with the potent small molecule agonist GI262570 (farglitazar) to design and synthesize a novel fluorescent and high-affinity probe for homogeneous and high-throughput fluorescent polarization (FP) assays. (psu.edu)
  • Development of high-throughput assays based on fluorescence polarization for inhibitors of the polo-box domains of polo-like kinases 2 and 3. (mpg.de)
  • This ebook outlines the basics of fluorescence polarization and discusses the advantages and limitations of FP-based biochemical assays. (drugtargetreview.com)
  • Historically, the screening and triaging of small molecule glycomimetics that bind to the galectin-3 CRD has been completed in fluorescence polarisation (FP) assays to determine K D values. (lu.se)
  • Our extensive SpectraMax® line of user-friendly microplate readers - the industry's most cited brand - provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation and include absorbance, fluorescence, and luminescence. (moleculardevices.com)
  • Single Molecule Spectroscopy laboratory (SMS lab) of Ivan Scheblykin is equipped with three wide-field fluorescence microscopes (M1, M2 and M3) allowing for luminescence imaging and spectroscopy of any sample from a single molecule and single particle to solutions and thin films. (lu.se)
  • The method is based on advanced measurements of luminescence and luminescence excitation polarization allows for monitoring of energy transfer in individual nanoantenna. (lu.se)
  • FPIA quantifies the change in fluorescence polarization of reaction mixtures of fluorescent-labelled tracer, sample antigen, and defined antibody. (wikipedia.org)
  • Samples were analyzed by an adapted fluorescence polarization immune assay (FPIA) method on the FLx analyzer (Abbott). (eur.nl)
  • Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric pressure flames", APPLIED PHYSICS B , vol. 69, 1999, pp. 477-485. (uni-bielefeld.de)
  • 2006. A fluorescence polarization assay for inhibitors of Hsp90 . (ub.edu)
  • A high-throughput assay based on fluorescence polarization for inhibitors of the polo-box domain of polo-like kinase 1. (mpg.de)
  • A high-throughput assay for signal transducer and activator of transcription 5b based on fluorescence polarization. (mpg.de)
  • Considering the lack of full details about the existing literature, this report describes an improved method of modified preparation for bone-containing metal implants for analysis by optical, fluorescence or polarization microscopy. (bvsalud.org)
  • Today it allows studying fluorescence, Raman scattering and even absorption signals coming from a single molecule using a powerful set of optical spectroscopy techniques. (lu.se)
  • Overhauser effect spectroscopy, dielectric relaxation and fluorescence spectroscopy. (lu.se)
  • The binding of fluorescein was measured in human plasma using four methods: steady-state dialysis, pressure dialysis, equilibrium dialysis, and fluorescence polarization. (jamanetwork.com)
  • Technical difficulties were encountered with the fluorescence polarization method above fluorescein concentrations of 10 -5 g/mL, but below this concentration, the method gave reproducible and reliable results compared with the three other methods. (jamanetwork.com)
  • Brubaker RF , Penniston JT , Grotte DA , Nagataki S. Measurement of Fluorescein Binding in Human Plasma Using Fluorescence Polarization. (jamanetwork.com)
  • When a fluorescent-labelled molecule (tracer) binds to another molecule the rotational motion will change, resulting in an altered intensity of plane-polarized light, which results in altered fluorescence polarization. (wikipedia.org)
  • The most well-known effect of this kind is fluorescence "blinking" - strong fluctuations of fluorescence intensity, polarization, or spectra of a single molecule or nanoparticle. (lu.se)
  • Observing that polarization increased with viscosity of the solvent and the size of the dye molecule, but decreased with an increase in temperature, he deduced that polarization increased with a decrease in mobility of the emitting species. (wikipedia.org)
  • The change in polarization is proportional to the amount of antigen in sample, and is measured by a fluorescence polarization analyzer. (wikipedia.org)
  • The solution is evaluated in a fluorescence polarization analyzer to gather a baseline measurement. (wikipedia.org)
  • The solution is evaluated again by the fluorescence polarization analyzer. (wikipedia.org)
  • This fluorescent analogue was designed to project the fluorophore into the adjacent protein cavity with minimal effects on receptor affinity and maximal effects on fluorescence polarization properties. (psu.edu)
  • Polarized light is a contrast-enhancing technique that improves the quality of the image obtained with birefringent materials when compared to other techniques such as darkfield and brightfield, differential interference contrast, phase contrast, Hoffman modulation contrast, and fluorescence. (microscopyu.com)
  • Condensers can also be replaced, and conversion kits are available to add phase contrast, polarization, fluorescence, or other capabilities to your current system. (fishersci.com)
  • While the key specificity of this assay is based on the aptamer's inherent affinity to membrane proteins on exosomes, exosomes' inherent huge mass/volume acts as mass-based fluorescence polarization amplifier. (rsc.org)
  • However, fluorescence brightness alone cannot discriminate micrometer-sized regions with high expression of non-aggregated proteins from regions where the proteins are aggregated on the molecular scale. (lu.se)
  • From 1925 to 1926 Francis Perrin detailed a quantitative theory for fluorescence polarization in multiple significant publications which remain relevant to this day. (wikipedia.org)
  • Then individual fluorescing molecules can be observed like "stars on the sky" in a fluorescence microscope (see the photo above). (lu.se)
  • This microscope is specially built for 2-dimentional polarization imaging (2DPOLIM). (lu.se)
  • The tracer and the specific antigen will compete to bind to the antibody and if the antigen is low in concentration, more tracer will be bound to the antibody resulting in a higher fluorescence polarization and vice versa. (wikipedia.org)
  • We introduced the concept of isosbestic nanoparticles in plasmonics, which characterizes nanoparticles whose photothermal properties are independent on the polarization, while the near-field enhancement is [27]. (fresnel.fr)
  • To determine whether the midtrimester serum lipid profile, as expressed by fluorescence polarization (FP) values is decreased in patients who subsequently develop pregnancy-induced hypertension (PIH). (tau.ac.il)
  • Fluorescence polarization immunoassays employ a fluorophore bound antigen that when bound to the antibody of interest, will increase fluorescence polarization. (wikipedia.org)
  • Free tracer in solution has a lower fluorescence polarization than antibody-bound tracer with slower Brownian motion. (wikipedia.org)
  • The fluorescence polarization value for the tracer containing solution is compared to the baseline and magnitude of difference is proportional to quantity of target analyte in sample. (wikipedia.org)