HybridizationSituChromosomesCytogeneticFISHGeneSpectralSpeciesMicroscopeTranslocationCellsPatientsAnalysisTestsCellSamplesChromosomeDiagnosed by karyotype analysisChromosomesCytogeneticsMetaphaseMolecularAncestral karyotypeMicroarrayGeneticConventionalAmnioticAnalysisSpecimensPrenatalDeletionNormalSyndromeTestPatients
Hybridization18
- FISH, or fluorescence in situ hybridization. (wikipedia.org)
- Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
- What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)
- Marrow samples can also be used for fluorescence in situ hybridization (FISH), flow cytometry, immunophenotyping, karyotype tests and polymerase chain reaction . (lls.org)
- CombiMatrix continues to offer other cytogenetic testing services, such as fluorescence in situ hybridization and molecular karyotyping, in addition to its core array-based offering. (genomeweb.com)
- We always start with the FISH [fluorescence in situ hybridization] and karyotype. (onclive.com)
- Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient's bone marrow. (hindawi.com)
- This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. (hindawi.com)
- We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30-35°N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. (bioone.org)
- In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)
- Here, we provide the first cytogenetic information on the species based on both conventional karyotyping and chromosomal mapping of 45S and 5S ribosomal genes through fluorescence in situ hybridization (FISH). (scielo.br)
- CombiMatrix performs genetic testing utilizing a variety of advanced cytogenomic techniques, including chromosomal microarray, standardized and customized fluorescence in situ hybridization ("FISH") and high-resolution karyotyping. (redchip.com)
- Bone marrow cells were examined by conventional karyotyping, fluorescence in situ hybridization (FISH) and mutation analysis at diagnosis and follow-up. (biomedcentral.com)
- del(20q) Fluorescence in situ hybridization using XL del(20q) probe (Metasystems, Germany) and Vysis LSI D20S108/20q12 probe showing 2 signals on normal A,B) and 1 signal on metaphase with deletion of 20q12 and/or 20qter (red signal) u2013 Courtesy Adriana Zamecnikova. (atlasgeneticsoncology.org)
- It is the recommended first-tier test for patients undergoing prenatal diagnosis for the indication of a fetal structural abnormality detected by ultrasound (unless the structural abnormality is strongly suggestive of a specific aneuploidy, in which case, karyotype with or without fluorescence in situ hybridization [FISH] may be offered before genomic microarray). (arupconsult.com)
- Fluorescence in-situ hybridization (FISH) assays on fresh (including Amniotic fluid, chorionic villi sampling, peripheral blood, product of conception, tumour tissue) and FFPE tissues. (kkh.com.sg)
- They were characterized by immunofluorescence, classical cytogenetics, p53 gene mutational analysis, fluorescence in-situ hybridization, and histopathological and immunohistochemical examinations of their xenografts, to demonstrate their potency of secondary transformation. (biomedcentral.com)
- Do not perform fluorescence in situ hybridization for myelodysplastic syndrome -related abnormalities on bone marrow samples obtained for cytopenias when an adequate conventional karyotype is obtained. (medscape.com)
Situ1
- In this paper, we demonstrate that the landmarks produced by cross-species fluorescence in situ hybridisation (FISH) of Brachypodium distachyon derived bacterial artificial chromosome (BAC) clones can be used for discrimination of Brachypodium pinnatum (2n = 18) chromosomes. (nih.gov)
Chromosomes5
- Analysis of the morphometric features of B. pinnatum chromosomes was performed to establish their total length, the position of centromeres, and the position of BAC-based landmarks in relation to the centromere, thereby enabling their effective karyotyping, which is a prerequisite for more complex study of the grass genome structure and evolution at the cytomolecular level. (nih.gov)
- Karyotyping human chromosomes by combinatorial multi-fluor FISH. (wikidata.org)
- The karyotypes of E. bicoloripes and E. saussurei , despite having the same chromosome number (2n=18+X0♂) had certain biarmed chromosomes ( FN =20♂ and FN =34♂ respectively). (pensoft.net)
- White (1973) reported a conservative karyotype consisting of 19 acrocentric chromosomes in males and 20 in females with X0♂/XX♀ sex chromosome system. (pensoft.net)
- Fluorescence-labeled DNA probes constructed from three whole house mouse (Mus domesticus) chromosomes were hybridized to metaphase spreads from deer mouse (Peromyscus maniculatus) to identify homologies between the species. (tamu.edu)
Cytogenetic2
- Cytogenetic Testing (Karyotyping). (lls.org)
- Poor cytogenetic studies of Pamphagidae were associated not only with the low density of their populations, but also with the uniformity of their karyotypes. (pensoft.net)
FISH1
- We propose the karyotype of this coral (2n = 30) based on C-banding and FISH analyses. (bioone.org)
Gene1
- However, for patients with a KMT2A rearrangement (KMT2A-r), the prognosis appears to depend on the fusion partner gene rather than the karyotype structure. (cancerindex.org)
Spectral1
- Case 1 was identified by spectral karyotyping. (bmj.com)
Species2
- The karyotypes of the remaining species differed from the ancestral karyotypes. (pensoft.net)
- In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level. (uaeu.ac.ae)
Microscope1
- Slides were stained with acridine orange and observed under a fluorescence microscope. (uaeu.ac.ae)
Translocation2
- A New Complex Karyotype Involving a KMT2A-r Variant Three-Way Translocation in a Rare Clinical Presentation of a Pediatric Patient with Acute Myeloid Leukemia. (cancerindex.org)
- G-banded karyotype showing deletion of 4p, derived from the mother, with balanced translocation (4p;8p). (medscape.com)
Cells1
- Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. (ca.gov)
Patients2
- Two patients with the clinical picture of Turner's syndrome showed a 46,XXp- and a 46,XXq- karyotype identified by a combination of fluorescence and autoradiography. (bmj.com)
- Patients with childhood acute myeloid leukemia (AML) with complex karyotypes (CKs) have a dismal outcome. (cancerindex.org)
Analysis1
- Karyotype analysis was performed by the G-banding technique. (hindawi.com)
Tests1
- These tests could consist of: Karyotyping. (wikipedia.org)
Cell1
- Karyotype and ultrastructure of a colony stimulating factor (CSF) producing cell line (5637) originated from a carcinoma of the human urinary bladder. (wikidata.org)
Samples1
- In a prospective study, all samples are classified according to the macroscopical phenotype, and ploidy is determined by karyotyping. (au.dk)
Chromosome8
- An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting. (github.com)
- No chromosome 22q11 microdeletion could be identified by fluorescence hybridisation with the Sc11.1 probe in any of the patients. (bmj.com)
- GenASIs HiSKY performs Hyperspectral Karyotyping analysis, leveraging the benefits of multiplexed spectral imaging to obtain a karyotype which uniquely colors each chromosome and provides a level of detailed analysis not available with any other karyotyping technique. (dssimage.com)
- This report highlighted the importance of the incorporation of conventional karyotyping and molecular genetic techniques in clinical practice, especially in prenatal diagnosis of uncommon chromosome abnormalities. (biomedcentral.com)
- Both parents had a normal karyotype, demonstrating the de novo origin of this ring chromosome. (biomedcentral.com)
- The proportion of abnormal karyotypes with mosaic decreased as gestational age increased, while the frequency of polyploidy and sex chromosome monosomy increased. (bvsalud.org)
- This study was conducted to obtain information about somatic chromosome numbers, karyotypes, and genome size of these five species. (ashs.org)
- The results show significant differences in nuclear DNA content, chromosome number, and karyotype among three native and two introduced lantana species and will help to identify, preserve, protect, and use native lantana species. (ashs.org)
Diagnosed by karyotype analysis1
- Down syndrome is easily diagnosed by karyotype analysis. (medscape.com)
Chromosomes6
- 18. Multicolor spectral karyotyping of rat chromosomes. (nih.gov)
- The great prevalence of biarmed chromosomes (the karyotypes of most species contain exclusively biarmed chromosomes) is a distinctive characteristic of Distichodontidae and Cithariniformes as a whole. (journaltocs.ac.uk)
- The parental karyotypes were normal, which indicated this mosaic case of isodicentric Y (idicY) chromosomes of the fetus was a de novo case. (biomedcentral.com)
- One of the fundamental techniques in cytogenetics is karyotyping , which involves the analysis and classification of chromosomes based on their size, shape, and banding patterns. (capricorn-scientific.com)
- In general, treating cells with KCl facilitates their expansion for optimal spreading of metaphase chromosomes during karyotyping procedures. (capricorn-scientific.com)
- The sample is diagnosed under karyotyping method, in which the number of chromosomes is counted. (syndromespedia.com)
Cytogenetics2
- Ninty-three patients with newly diagnosed CLL were tested by five probes including RB1 (13q14.1), D13S25 (13q14.3), p53(17p13.1), ATM( 11q22.3) and CSP12, while conventional cytogenetics (CC) was used for karyotype analysis. (nih.gov)
- The study protocol involved halving each specimen collected from these procedures, with a single cytogenetics lab performing all karyotyping. (aacc.org)
Metaphase2
- Karyotyping of 100 metaphase lymphocytes from peripheral blood was performed by conventional techniques. (medscape.com)
- KCl is an essential component in the preparation of metaphase spreads for karyotyping. (capricorn-scientific.com)
Molecular1
- 46,XX karyotype, and molecular analysis by quantitative fluorescence PCR did not show the presence of a second cell line of maternal origin. (cdc.gov)
Ancestral karyotype1
- In the four genomes discussed here, our results show that all autosomes have a clear ortholog in the ancestral karyotype. (bvsalud.org)
Microarray4
- Wapner was the principal investigator of a recent landmark clinical trial that compared microarray analysis against karyotyping in more than 4,000 women referred for prenatal diagnosis based on maternal age, abnormal Down syndrome screening results, anomalies identified on ultrasound, or other indications (N Engl J Med 2012;367:2175-84). (aacc.org)
- After testing of more than 3,100 cases, the authors found that microarray analysis had an additional 8.2% diagnostic yield in comparison to karyotyping. (aacc.org)
- The researchers also found that microarray results generally were available sooner than those of karyotypes, owing to most microarray specimens not needing to be cultured, which is necessary for karyotyping. (aacc.org)
- Graham noted that chromosomal microarray analysis is "considered by many to provide improved resolution and accuracy over traditional karyotyping. (genomeweb.com)
Genetic3
- Amniocentesis involves taking a sample of the amniotic fluid in order to perform a karyotype test and diagnose any genetic defects that may affect the fetus. (unboundmedicine.com)
- In prenatal genetic analysis, CMA has been recommended to patient with fetal structural anomalies and/or stillbirth instead of fetal karyotype [ 4 ]. (biomedcentral.com)
- CONCLUSIONS: The combined application of CMA and karyotyping were recommended in prenatal diagnosis for providing a scientific and accurate genetic diagnosis and improving the quality of prenatal genetic counseling. (bvsalud.org)
Conventional1
- Authors : Richard Mollard, Michael Mahony : The karyotype of the IUCN least concern red-backed toadlet Pseudophryne (P.) coriacea (Keferstein, 1868) from the New South Wales Central Coast is described following tissue culture of toe clipping macerates and conventional DAPI staining. (journaltocs.ac.uk)
Amniotic1
- A simple and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for identification and quantification of eleven selected bisphenols in amniotic fluid samples is proposed. (unboundmedicine.com)
Analysis2
- This is the first described cell culture of a species from the genus Pseudophryne Fitzinger, 1843, first published analysis of the P. coriacea karyotype and the first published analysis of centromeric allocation of this genus. (journaltocs.ac.uk)
- For years, CMA technology has been proved to be equivalent to karyotype analysis in detection of common aneuploidies [ 5 ]. (biomedcentral.com)
Specimens1
- To confirm the Nannaethiops Günther, 1872 and Neolebias Steindachner, 1894 species identification, mt-DNA sequences of the two markers (COI and 16S rRNA) were obtained from karyotyped specimens and compared with the relevant sequences accessible from GenBank. (journaltocs.ac.uk)
Prenatal2
- For nearly 50 years, karyotyping has been the backbone of prenatal diagnostic testing for congenital anomalies, but rapid advances in genomic technologies are outpacing both this tried-and-true method and others poised to take its place. (aacc.org)
- This is a big step forward, monumental even, because prenatal diagnostics have been focused for so long on Down syndrome detected by karyotyping, which is a very crude way to look at someone's genome," he explained. (aacc.org)
Deletion2
- Fluorescence signals were then quantified and analyzed by a combination of the ADM2 algorithm from Agilent and a custom software developed to detect CNVs greater than 3Mb and of 24 specific and well-characterized deletion/duplication regions. (enzolifesciences.com)
- G-banded karyotype showing deletion of 4p, derived from the mother, with balanced translocation (4p;8p). (medscape.com)
Normal2
- All patients had a normal karyotype. (bmj.com)
- Additionally, microarrays detected clinically relevant deletions or duplications in 6% of samples referred due to anomalies on ultrasound and in 1.7% referred because of advanced maternal age or positive screening results, all of which had normal karyotype results. (aacc.org)
Syndrome2
- RÉSUMÉ La présente étude a examiné les cellules souches hématopoïétiques de 19 cas de syndrome myélodysplasique de haut risque à la recherche de signaux apoptotiques et anti-apoptotiques et de proliférations cellulaires et a établi un lien entre ces derniers et les sous-types cytogénétiques et cliniques, en particulier la trisomie 8. (who.int)
- Les cas de syndrome myélodysplasique de haut risque avaient un pourcentage nettement supérieur de cellules CD34+ apoptotiques et de survivine+ anti-apoptotiques par rapport aux témoins, en particulier dans les cas atteints de trisomie 8. (who.int)
Test1
- She cited the results of a US National Institute of Child Health and Human Development-funded study that demonstrated that arrays provide more clinically relevant information than older methods and suggested that CMA replace karyotyping as a first-tier test. (genomeweb.com)
Patients1
- Discussion: Overall, these findings provide important insights into the understanding of spontaneous abortion and have implications for the development of personalized interventions for patients with abnormal karyotypes. (bvsalud.org)