• Cell nuclei were staiend by DAPI (blue color fluorescence). (figshare.com)
  • These are often used in conjunction with a nuclear counterstain, such as DAPI, which enables identification of all cells in the image. (moleculardevices.com)
  • Fluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. (dojindo.com)
  • DAPI stained liver nuclei. (bucher.ch)
  • DAPI-labeled nuclei of the malaria parasite Plasmodium falciparum inside of human red blood cells imaged using fluorescence microscopy. (zeiss.com)
  • The cells are counter-stained by DAPI to visualize nuclei and stained coverslips then are mounted to slides and visualized under fluorescence microscope. (asn-online.org)
  • A) Immunofluorescence imaging showing cell nuclei (DAPI, blue) and BAFF (green) from bronchoalveolar lavage (BAL) cells of WT mice exposed or not to CS. (univ-orleans.fr)
  • DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. (sigmaaldrich.com)
  • The cell nuclei is stained with DAPI (blue). (lu.se)
  • Primary cultured HTFs were incubated with transforming growth factor β1 (TGF-β1) alone or combined with nintedanib, and cell proliferation and migration were measured by cell counting kit-8 (CCK8) and the scratch wound assay, respectively. (molvis.org)
  • Cell viability was analyzed using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2h-tetrazolium bromide assay. (molvis.org)
  • This white paper outlines an approach developed to address these limitations: an integrated assay solution that is based on Incucyte ® Live-Cell Analysis, along with a novel pH-sensitive dye coupled antibody fragment (Incucyte ® FabFluor Antibody Labeling Reagents). (news-medical.net)
  • These esterase substrates, therefore, can serve as assay probes of cell viability. (dojindo.com)
  • Two such examples are fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA). (aacrjournals.org)
  • Fungal load, polymorphonuclear neutrophil infiltration, and the production of related proteins were determined using colony plate counting, in vivo confocal microscopy, periodic acid-Schiff staining, fungal fluorescence staining, immunofluorescence staining, myeloperoxidase assay, and Western blotting. (bvsalud.org)
  • Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
  • The 2016 National Comprehensive Cancer Network (NCCN) Clinical Practice Guidelines also recommend the use of serum free light chain assay and fluorescence in situ hybridization (FISH) for del 13, del 17p13, t(4;14), t(11;14), 1q21 amplification as part of the initial diagnostic workup. (medscape.com)
  • Here, fluorescently stained cell nuclei were identified in the green fluorescence imaging channel. (moleculardevices.com)
  • Fluorogenic esterase substrates that can be passively loaded into viable cells, such as Calcein-AM, BCECFAM, Carboxyfluorescein succinimidyl ester (CFSE), and Fluorescein diacetate (FDA), are converted by intracellular esterases into fluorescein analogs with green fluorescence. (dojindo.com)
  • One molecule of AO can intercalate with three base pairs of double-stranded DNA to emit green fluorescence with the maximum wavelength at 526 nm. (dojindo.com)
  • In order to verify this hypothesis, we treated HTC cells with 1-10 mM Roundup and analysed cellular features by flow cytometry, fluorescence and electron microscopy. (biosafety-info.net)
  • Slide-based cytometry combines image acquisition and data treatment, and associates the power of flow cytometry (FCM) and the resolution of the microscopic studies making it suitable for the analysis of cells with rare phenotype. (nih.gov)
  • Although these cells represent only about 1% of the total population as calculated by flow cytometry, they can be identified in the culture plate with high precision by microscopy. (nih.gov)
  • It is common practice for laboratories to use interphase fluorescence in situ hybridization (FISH) panels in conjunction with karyotyping for the detection of the most prognostically significant recurrent AML-associated cytogenetic abnormalities, both in diagnostic specimens and during follow-up to monitor response to therapy. (medscape.com)
  • Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)
  • However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
  • A cell membrane is typically labeled with a fluorescent dye to allow for observation. (wikipedia.org)
  • Before photobleaching can occur, cells must be injected with a fluorescent protein, often a green fluorescent protein (GFP), which will allow the targeted proteins to fluoresce and therefore be followed throughout the process. (wikipedia.org)
  • Analyses using StainFree and fluorescent nuclear dye to identify the cells were compared and results evaluated using SoftMax ® Pro Software. (moleculardevices.com)
  • This reagent labels active mitochondria in live cells and fluoresces at a far-red wavelength that is detected by the red fluorescent channel of the MiniMax cytometer. (moleculardevices.com)
  • Cells were imaged in the transmitted light (TL) channel of the MiniMax cytometer to obtain cell counts and assess morphology, and in the red fluorescent channel to measure mitochondrial activity. (moleculardevices.com)
  • In the first, individual cells were identified using StainFree analysis, which allows identification of individual cells in the transmitted light images without the need for fluorescent nuclear staining. (moleculardevices.com)
  • For each cell identified, red fluorescent signal representing mitochondrial staining was measured and captured as an output parameter in the software. (moleculardevices.com)
  • Visualization of a cell with fluorescent compounds provides a wide variety of information for the analysis of cell functions. (dojindo.com)
  • Various activities and structures of a cell can be targeted for staining with fluorescent compounds (Fig. 1). (dojindo.com)
  • Succinimidyl ester compounds can also be used to improve retention of the fluorescent derivative within the cell. (dojindo.com)
  • Nucleotide staining with fluorescent intercalators is mostly used for dead cell detection. (dojindo.com)
  • Once converted into fluorescent products by esterase, these compounds are retained by cells because of their negative charges. (dojindo.com)
  • Fluorescent esterase substrates may also be used in cell viability assays in place of tetrazolium analogs such as MTT or WST. (dojindo.com)
  • Cell membranes of viable cells are impermeable to these fluorescent dyes,except for the Hoechst dyes, and these dyes can therefore be used as fluorescent indicators of dead cells. (dojindo.com)
  • Fluorescent staining using CTC is another method used to detect viable bacterial cells. (dojindo.com)
  • The rapid expansion in biomedical research using live-cell imaging techniques over the past several years has been fueled by a combination of events that include dramatic advances in spinning disk confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. (fsu.edu)
  • Fluorescent Protein Technology - It took over thirty years, and the advent of recombinant DNA as well as vastly improved molecular biological approaches to see the pioneering work of Osamu Shimomura developed into a useful tool for live-cell imaging by Doug Prasher and Martin Chalfie. (fsu.edu)
  • The adherent cells were labeled by the fluorescent dye Hoechst 33342. (nih.gov)
  • [ 64 ] Screening for PNH involves detection of deficiency of glycosylphosphatidyl-inositol (GPI) anchored proteins, such as CD14, CD16 and CD24, as well as fluorescent aerolysin (FLAER) for white blood cells, and CD55 and CD59 for red cell analysis, on flowcytometry. (medscape.com)
  • Cell permeable fluorescent minor groove-binding probe for DNA. (sigmaaldrich.com)
  • When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces. (sigmaaldrich.com)
  • Fluorescent labeling of the nucleus (yellow) and actin (red) makes it possible to see much more detailed cell structure. (thermofisher.com)
  • Positively charged molecules are usually cell membrane permeable and accumulate in mitochondria. (dojindo.com)
  • Mitochondria exist in most eukaryotic cells and play a very important role in oxidative metabolism by generating ATP as an energy source. (dojindo.com)
  • The average number of mitochondria per cell is from 100 to 2,000. (dojindo.com)
  • Although the typical size is about 0.5-2 mm, the shape, abundance, and location of mitochondria vary by cell type, cell cycle, and cell viability.Therefore, visualization of mitochondra is important. (dojindo.com)
  • MitoRed and Rh123 readily pass through cell membranes and accumulate in mitochondria. (dojindo.com)
  • The fluorescence intensity of Rh123 reflects the amount of ATP generated in mitochondria. (dojindo.com)
  • As the poly-dimethylsiloxane (PDMS) microdevices presented in this manuscript are optically transparent and air permeable, we can culture living cells inside the device and observe their growth behaviors under a microscope. (jove.com)
  • Acquired with a standard wide field fluorescence microscope (left half) and an exposure of the same sample taken with a THUNDER imager (right half). (news-medical.net)
  • Inverted fluorescence microscope (Olympus, Tokyo, Japan) was used for imaging. (spandidos-publications.com)
  • The images of cell populations were collected by a two-photon microscope and processed by a software developed by us. (nih.gov)
  • b ) CD68 expression was quantified based on fluorescence intensity. (figshare.com)
  • Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). (researchsquare.com)
  • B) Quantification of fluorescence intensity. (univ-orleans.fr)
  • The software allows the automated segmentation of the nuclei in a very dense cell environment, the measurement of the fluorescence intensity of each nucleus and the recording of their position in the plate. (nih.gov)
  • The cells with a given fluorescence intensity can then be located easily on the recorded image of the culture plate for further analysis. (nih.gov)
  • Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same time, the method associates the advantage of the FCM analysis and the microscopic observation. (nih.gov)
  • Two of the other, less frequently employed uses of FLIP are to determine how proteins are shuttled from the Cytoplasm to the Nucleus and then determine the rate at which this shuttling occurs. (wikipedia.org)
  • The sooner a part of the cytoplasm is used in the shuttling process, the more rapidly it experiences complete loss of fluorescence. (wikipedia.org)
  • If the cell also participates in nuclear export from the nucleus to the cytoplasm, photobleaching will also occur in the nucleus. (wikipedia.org)
  • The exchange rate between the nucleus and cytoplasm can also be determined from this type of data. (wikipedia.org)
  • RAF-MEK-ERK signalling is normally thought to occur in the cytoplasm of the cell. (bath.ac.uk)
  • This endogenous metabolite of the branched-chain amino acid (BCAA) leucine (LEU) is produced when leucine is oxidised in the cell cytoplasm, mainly in the liver and muscles. (researchsquare.com)
  • Note the blue cytoplasm, eccentric nucleus, and perinuclear pale zone (or halo). (medscape.com)
  • Cell apoptosis was analyzed by Hoechst 33258 staining and annexin V-PI labeling. (molvis.org)
  • Hoechst dyes are positively charged under physiological conditions and can pass through viable cell membranes. (dojindo.com)
  • Notch signaling is highly conserved cell signaling pathway, which is involved in diverse embryonic organs or tissue development as well as regeneration ( 1 - 10 ). (frontiersin.org)
  • The following were grown in 75 cm 2 tissue culture treated flasks: BT-474 cells (DMEM+10% FCS), HT1080 cells (F12K+10% FCS, 1% glutamax, 1 u/mL pen/strep), Jurkat and Raji cells (both RPMI 1640+10% FCS). (news-medical.net)
  • THUNDER Imagers allow to visualize clearly in real time details even deep inside thick samples of biologically relevant models like model organisms, tissue sections, and 3D cell cultures. (news-medical.net)
  • Naive CD4 + T cells can develop into various helper T (Th) subsets with different cytokine profiles and play a discriminative role in translating antigen-specific immune responses into tissue functions or immunopathology. (atsjournals.org)
  • The study of two cases of ductal carcinoma in situ in breast tissue supports the biological observation regarding the existence of a preferential intraductal invasion, and therefore a common origin, between the primary tumor and the gene amplification in the cell-nuclei lining the ductal structures in the breast. (nih.gov)
  • The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. (bucher.ch)
  • From tissue to single cells or nuclei in minutes with walk-away operation. (bucher.ch)
  • The Singulator™ 100 enables rapid and hands-off tissue dissociations, making it easy for researchers to reproducibly prepare suspensions of nuclei or highly viable cells from small samples in high yield, for a wide range of single-cell analyses. (bucher.ch)
  • The Singulator 100 overcomes the challenges with manual tissue preparation methods by producing consistent cell or nuclei isolations from a variety of solid tissue samples, reducing hours of hands-on processing to minutes. (bucher.ch)
  • S2 is developing single cell spatial sequencing technology that will capture the spatial location, i.e., 3-dimensional coordinates, of single cells in tissue for NGS readout. (bucher.ch)
  • Get single cells or nuclei from solid tissue in minutes. (bucher.ch)
  • The long-circulating and higher tumor accumulation of E/PCF-NPs resulted in complete ablation of breast tumor tissue through the enhanced photothermal effect by NIR laser irradiation-mediated cell apoptosis. (dovepress.com)
  • SFTs of the pleura are localized mesenchymal neoplasms composed of fibroblastlike cells believed to arise from the subpleural connective tissue. (medscape.com)
  • Under these experimental conditions, the death rate and the general morphology of HTC cells were not affected, as well as most of the cytoplasmic organelles. (biosafety-info.net)
  • In fundamental cell biological processes such as signal transduction, enzymatic activity or intracellular fusion during vesicular transport, a large number of proteins are recruited to and released from the cytoplasmic surface of intracellular membranes (Casey et al. (biologists.com)
  • This enabled red mitochondrial fluorescence to be quantified and reported by the software for the cytoplasmic area around the nucleus. (moleculardevices.com)
  • DSRCT is composed of small cells with round hyperchromatic nuclei and a dense fibrous or spindle cell stroma. (medscape.com)
  • THUNDER Imager for 3D Cell Culture & 3D Live Cell are designed for imaging of cell culture assays. (news-medical.net)
  • In addition, cell apoptosis was measured by double staining, DNA fragmentation and annexin V-fluorescein isothiocyanate binding assays. (ijpsonline.com)
  • The cell viability, reactive oxygen species (ROS) levels, ratios of oxidized nicotinamide adenine dinucleotide to its reduced form (NAD + /NADH), apoptosis assays, and cellular uptake of E/PCF-NPs were determined on 4T1 cells. (dovepress.com)
  • Although we cannot exclude that other factors than the presence of the herbicide residues could be responsible for the cellular modifications described in GM-fed mice, the concordance of the effects induced by low concentrations of Roundup on HTC cells suggests that the presence of Roundup residues could be one of the factors interfering with multiple metabolic pathways. (biosafety-info.net)
  • Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. (frontiersin.org)
  • Antibody-drug conjugates (ADCs) distribute cytotoxic payloads to cells with the degree and specificity of cellular uptake being crucial to the molecule's effectiveness and safety. (news-medical.net)
  • In the present study, we analyzed the proliferation and apoptosis of trophoblasts and human decidual cells in patients with recurrent spontaneous abortion and the related cellular pathway mechanism. (spandidos-publications.com)
  • This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
  • Ester can pass through viable cell membranes, where it is hydrolyzed by cellular esterases into a negatively charged molecule under physiological conditions. (dojindo.com)
  • Cellular immunity, particularly of CD4 + T cells, IFN-γ and tumor necrosis factor (TNF)-α, has a central role in the control of and protection against Mycobacterium tuberculosis (MTB) infection ( 1 ). (atsjournals.org)
  • Autophagy and apoptosis are two forms of programmed cell death, which are essential for cellular homeostasis. (ijpsonline.com)
  • Loss of fluorescence is defined by the mobile fraction, or the fraction of fluorophores capable of recovering into a photobleached area, of the fluorescently labeled protein. (wikipedia.org)
  • We captured the dynamic changes in the shape of a fluorescently labeled vegetative nucleus and sperm cells in a pollen tube during this process. (jove.com)
  • If shuttling occurs at a rapid pace, the fluorescence levels within the nuclear compartments will decrease rapidly through the frames taken. (wikipedia.org)
  • To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)
  • 7 implemented digital scanned laser light-sheet fluorescence microscopy in combination with incoherent structured-illumination microscopy (DSLM-SI) and performed nuclear segmentation of time-series images acquired by DSLM-SI. (nature.com)
  • Nuclear GFP-ΔBRAF retains MEK/ERK activating potential and is associated with the accumulation of phosphorylated MEK and ERK in the nucleus. (bath.ac.uk)
  • Once the cells have been identified via their nuclear stain in one fluorescence channel of an imaging system, their mitochondrial activity can be measured on a per-cell basis as fluorescence in a second channel corresponding to the specific mitochondrial dye used. (moleculardevices.com)
  • StainFree ™ Technology from Molecular Devices frees researchers from the inconvenience of nuclear counterstaining by identifying cells imaged with the transmitted light channel of the SpectraMax ® MiniMax ™ 300 Imaging Cytometer. (moleculardevices.com)
  • however, nuclear accumulations of pSMAD2-C and CLDN3 redistribution were observed with similar time course in ciliated and non-ciliated cells. (springer.com)
  • While diffusion appears to play a crucial role in nuclear dynamics and RNA transport, some data argue for a model in which diffusion is controlled, at least in part, by the organization of the nucleus in well-defined compartments. (cnrs.fr)
  • These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation and DNA fragmentation [ 14 ]. (ijpsonline.com)
  • With a smaller group of undergraduate students who take research for credit, we are also able to venture into fluorescence microscopy using fixed cells and nuclear dyes. (zeiss.com)
  • To address this issue, we have designed an integrated image analysis software system for high-throughput segmentation of nuclei. (nih.gov)
  • This study was designed to examine whether the unconventional spicing of XBP1 mRNA could occur in the nucleus and its possible biological relevance. (mdpi.com)
  • These UCNPs allow programmable activation of two synergistic light-gated ion channels VChR1 and Jaws in the same cell to manipulate membrane polarization, demonstrated here for cardiac pacing. (nature.com)
  • This occurs several times, to ensure that all accessible fluorophores are bleached since unbleached fluorophores are exchanged for bleached fluorophores, causing movement through the cell membrane. (wikipedia.org)
  • Notch receptors are processed in the endoplasmic reticulum and Golgi apparatus within the signal-receiving cell, through cleavage and glycosylation, generating a Ca 2+ -stabilized heterodimer composed of NECD non-covalently attached to the transmembrane NICD inserted in the membrane called S1 cleavage. (frontiersin.org)
  • This effect was observed well before any significant membrane damage and cell death. (jneurosci.org)
  • Since it has an amine-reactive succinimidyl group, fluorescein derived from CFSE can covalently bond to proteins or other amino groups in the cell or on the cell membrane. (dojindo.com)
  • Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
  • Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
  • Solanum nigrum (SN) is a herb with anticancer effects such as destruction of tumor cell membrane, induction of apoptosis by NF-kappaB, caspase activation, nitric oxide production and inhibition of angiogenesis [ 3 ]. (ijpsonline.com)
  • Autophagy, the type II cell death, is a physiological mechanism involving degradation of intracellular damaged proteins and organelles into membrane vacuoles. (ijpsonline.com)
  • IMCD-3 cells express AQP2 with an even distribution in cytosol and membrane in untreated control cells. (asn-online.org)
  • Similar to vasopressin, treatment of IMCD-3 cells with imatinib also induced a profound membrane distribution. (asn-online.org)
  • Oxidative stress and reactive oxygen species (ROS), which are majorly sourced from macrophages [ 5 ], may be the major contributory factors leading to testicular cell apoptosis and acute inflammation of testes [ 6 ]. (hindawi.com)
  • TUNEL staining was used to investigate apoptosis in synovial cells. (hindawi.com)
  • Sparstolonin B was showed to alleviate joint swelling in RA rats, inhibited inflammatory cell infiltration and the release of ROS, reduced damage caused by oxidative stress, and suppressed the rate of apoptosis in synovial cells. (hindawi.com)
  • Pyroptosis is a newly discovered form of cell death that differs from apoptosis and necrosis [ 6 ]. (hindawi.com)
  • During apoptosis, apoptotic bodies are formed within cells. (hindawi.com)
  • This study is to investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in tert -butyl hydroperoxide ( t BHP)-induced apoptosis of human trabecular meshwork (iHTM) cells. (molvis.org)
  • Activation of p38MAPK plays an important role in t BHP-induced apoptosis of iHTM cells. (molvis.org)
  • Further study on the mechanisms of p38MAPK in human TM cell apoptosis may help to illuminate the pathogenesis of POAG. (molvis.org)
  • The mechanism of recurrent abortion is considered to be related to the proliferation and apoptosis of human decidual cells and cytotrophoblasts ( 4 ). (spandidos-publications.com)
  • TP53 protein can induce cell growth arrest, apoptosis, differentiation and DNA repair, and activate or inhibit the expression of downstream genes such as Bax and CDKN1A ( 5 ). (spandidos-publications.com)
  • In the present study, we comparatively analyzed the cell apoptosis and cell signaling pathways of healthy patients and those with recurrent spontaneous abortion, providing a theoretical basis for clinical treatment. (spandidos-publications.com)
  • The absence of NuRD triggers apoptosis via the EGL-1-CED-9-CED-4-CED-3 pathway, while an ectopic gain of NuRD enables apoptotic daughter cells to survive. (elifesciences.org)
  • In this investigation, the role of autophagy and apoptosis in Solanum nigrum fruit extract-induced cytotoxicity in MDA-MB-468, a triple negative breast cancer cell line was studied. (ijpsonline.com)
  • These findings indicated that Solanum nigrum extract induced cell death in MDA-MB-468 cells by two distinct mechanisms, apoptosis and autophagy, and these findings further suggest a possibility that this extract could be used to treat triple negative breast cancer. (ijpsonline.com)
  • A previous study reported that the extract of the whole plant of SN activated cell death in hepatoma cells through autophagy and apoptosis [ 7 ]. (ijpsonline.com)
  • Treatment with E/PCF-NPs under irradiation induced 4T1 cell apoptosis in approximately 93.6% cells. (dovepress.com)
  • Now, maturing sample preparation methods are delivering more reliable single-nucleus RNA sequencing (snRNA-seq), letting researchers analyze cells in previously intractable tissues. (genomeweb.com)
  • These developments come as single-cell methods are increasingly applied in translational or clinical research. (genomeweb.com)
  • Single-cell methods took off in the next few years, but it took until 2017 for snRNA-seq to arrive. (genomeweb.com)
  • Single-nucleus methods aren't without drawbacks. (genomeweb.com)
  • The device offers advantages over other optical biopsy methods that use fluorescence, which is not as specific of an indicator, according to Wax. (mddionline.com)
  • Development of novel tools and methods for studying lysosome-dependent cell death Artificial intelligence applications in biomedical science 1. (lu.se)
  • Increasing evidences indicate that the imbalance among these pro- and anti-inflammatory molecules, such as interleukin-1 β (IL-1 β ), IL-6, tumor necrosis factor- α (TNF- α ), and IL-10, in testicular cells can result in orchitis [ 3 , 4 ]. (hindawi.com)
  • Additionally, monoclonal antibodies are utilized within immunotherapy to flag tumor cells for immune cell clearance (e.g. (news-medical.net)
  • IL-1β, IL-6, and/or tumor necrosis factor-α promoted Th22-cell differentiation from CD4 + T cells. (atsjournals.org)
  • Statistical analysis of genetic changes within cell nuclei that are far from the primary tumor would help determine whether such changes have occurred prior to tumor invasion. (nih.gov)
  • To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor requires quantitative evaluation of a large number of cell nuclei from continuous meaningful structures such as milk-ducts, tumors, etc., located relatively far from the primary tumor. (nih.gov)
  • One of the hallmarks of breast cancer is resistance of tumor cells to cell death. (ijpsonline.com)
  • 0.01% HCLO reduced the severity of A. fumigatus keratitis in rats by decreasing the clinical score, fungal loading (periodic acid-Schiff, plate count, and fungal fluorescence staining), and inhibiting neutrophil infiltration and activity (immunofluorescence staining and myeloperoxidase). (bvsalud.org)
  • Cyclic, uniaxial stretch (10%, 1 Hz) of human VSMCs caused Notch3 activation, demonstrated by a stretch-induced increase in hairy and enhancer of split 1/hairy-related transcription factor-1 expression, translocation of Notch3 to the nucleus, and a decrease in the Notch3 extracellular domain. (cdc.gov)
  • Single nucleus sequencing really becomes applicable when the user cannot for whatever reason isolate their cells without damaging them," such as with fixed or frozen samples, he added. (genomeweb.com)
  • A nucleus contains only about 1 percent of total RNA inside a cell, but single-nucleus transcriptomics works because that 1 percent is enough to detect the majority of cell populations in a sample. (genomeweb.com)
  • Immobility indicates that there are proteins that may be in compartments closed off from the rest of the cell, preventing them from being affected by the repeated photobleaching. (wikipedia.org)
  • The most commonly stained cell components are cell membranes, proteins, and nucleotides. (dojindo.com)
  • These compounds are neutral molecules that pass through cell membranes and covalently conjugate with cell proteins. (dojindo.com)
  • Qualitative and quantitative analyses of the rare phenotypic variants in in vitro culture systems is necessary for the understanding of cell differentiation in cell culture of primary cells or cell lines. (nih.gov)
  • FISH is targeted toward specific abnormalities, and results can be evaluated in an automated fashion on interphase nuclei, allowing for examination of more cells than a traditional karyotype. (medscape.com)
  • Speckled pattern distributed throughout the interphase nucleus with characteristic heterogeneity in the size, brightness and distribution of the speckles. (cdc.gov)
  • He wanted to sequence RNA in single pancreas cells, but the organ, which produces digestive enzymes, was filled with RNases that could ruin the very molecules he was hoping to study. (genomeweb.com)
  • Fluorescence Loss in Photobleaching (FLIP) is a fluorescence microscopy technique used to examine movement of molecules inside cells and membranes. (wikipedia.org)
  • Small neutral molecules and positively charged molecules can pass through viable cell membranes and remain inside of cells, depending on their reactivity or hydrophilicity. (dojindo.com)
  • Negatively charged molecules cannot pass through viable cell membranes. (dojindo.com)
  • Covalently conjugated molecules can stay in the cell for several weeks. (dojindo.com)
  • Calcein and BCECF can be converted into electrically neutral molecules by the addition of acetyl or acetoxymethyl groups to their phenolic OH or carboxylic groups, which allows them to freely permeate into the cell. (dojindo.com)
  • And startups such as Dolomite Bio and LevitasBio are keying in on nuclei isolation or cleanup as applications for their hardware. (genomeweb.com)
  • The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs. (bucher.ch)
  • Ester is a suitable functional group for staining viable cells. (dojindo.com)
  • Several fluorescein analogs with ester groups in their structure are available for staining viable cells. (dojindo.com)
  • tetrazolium salts detect dehydrogenase activity of viable cells. (dojindo.com)
  • It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
  • In particular, PMCs were able to function as antigen-presenting cells to stimulate CD4 + T-cell proliferation and Th22-cell differentiation. (atsjournals.org)
  • Pleural mesothelial cells stimulate CD4 + T-cell proliferation and Th22 cell differentiation in response to tuberculosis antigen. (atsjournals.org)
  • First described in 1848, MM is characterized by a proliferation of malignant plasma cells and a subsequent overabundance of monoclonal paraprotein (M protein). (medscape.com)
  • The expression levels of autophagy-related genes, Lc3 and Beclin1 increased in cells treated with 1.5 mg/ml of Solanum nigrum extract. (ijpsonline.com)
  • Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. (bucher.ch)
  • Overall, snmC-seq allows for the large-scale multiplex sequencing of sorted neuronal nuclei that is needed to gain insight into the diverse cell types of the brain. (epigenie.com)
  • Continuity of the Golgi apparatus, endoplasmic reticulum, and nucleus have been verified using FLIP. (wikipedia.org)
  • Most important, the probe can specifically mark the nuclei of HeLa and A549 cells, respectively. (houstonmethodist.org)
  • HeLa cell spheroid stained with Alexa Fluor 568 Phalloidin (Actin) and YOYO 1 iodide (Nucleus). (news-medical.net)
  • The peptide-Cu cluster probe, mainly composed of Cu 14 , emitted blue two-photon fluorescence under femtosecond laser excitation. (houstonmethodist.org)
  • In addition, one system has Fluorescence lifetime and polarization imaging capabilities, the other system has near IR detection channel for GaInP nanowire imaging and 405 nm excitation for cell nucleus imaging. (lu.se)
  • 10x Genomics had a so-called "demonstrated protocol" for isolating peripheral blood mononuclear cell nuclei, so he tried throwing his pancreas samples into it. (genomeweb.com)
  • Several drug therapies are valuable in the treatment of symptomatic MM. Clinicians treat many patients with high-dose therapy and peripheral blood or bone marrow stem cell transplantation. (medscape.com)
  • We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. (frontiersin.org)
  • Regulation of arteriovenous specification and differentiation in both endothelial cells and vascular smooth muscle cells are also involved in Notch signaling including regulation of blood vessel sprouting, branching during normal and pathological angiogenesis, and the physiological responses of vascular smooth muscle cells ( 4 , 6 , 7 , 21 - 23 ). (frontiersin.org)
  • Internalization of antibodies by specific (target mediated) or non-specific (e.g. pinocytosis in endothelial cells) mechanisms forms a key determinant of the half-life in the body, and even subtle changes to the structure of monoclonal antibodies can result in considerable changes to the duration of activity. (news-medical.net)
  • Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
  • 1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
  • THUNDER Imager eliminates the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. (news-medical.net)
  • Nuclei of the fixed cells were counter-stained with EarlyTox Dead Green Dye (Molecular Devices cat. (moleculardevices.com)
  • However, when labeled antibodies are added to cells, a fluorogenic signal can be seen as the Fab-Ab complex is internalized and then processed via acidic (pH 4.5-5.5) endosomes and lysosomes (Figure 1). (news-medical.net)
  • Understanding the role of lysosomes in diseases with altered cell death 3. (lu.se)
  • However, differentiation and immune regulation of Th22 cells in tuberculous pleural effusion (TPE) remain unknown. (atsjournals.org)
  • The impacts of proinflammatory cytokines and antigen presentation by pleural mesothelial cells (PMCs) on Th22-cell differentiation were explored. (atsjournals.org)
  • Epigenetic regulation of gene expression is an essential mechanism that guides cell differentiation during development. (elifesciences.org)
  • Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses. (bucher.ch)
  • Then our data becomes pestered with a lot of signals that have nothing to do with the biology at the time of harvesting those cells. (genomeweb.com)
  • Currently unique to the market, the new technology deployed in THUNDER Imagers makes visualization and analysis of large volume, thick specimens ideal for many biomedical applications where they are required, including regenerative medicine, cancer, and stem cell research to decode 3D biology in real time. (news-medical.net)
  • S2 Genomics is developing integrated sample preparation systems for processing tissues into genomic samples for single-cell genomics and cell biology studies. (bucher.ch)
  • In this paper we develop a method that applies these principles to a particularly hot problem in cell biology, the study of stem cell like cells in cultures of primary cells, cancer cells, and various cell lines. (nih.gov)
  • Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues. (bucher.ch)
  • Data to show the pH sensitivity of the labeling probe (B and C) . Note the relatively low fluorescence of FabFluor at pH 7.0. (news-medical.net)
  • Upon phosphorylation, R-SMADs recruit the co-SMAD SMAD-4 to form protein complexes that translocate into the nucleus and exhibit transcriptional activity. (springer.com)
  • Once Notch is activated, the NICD is cleaved by γ-secretase and translocated into the nucleus ( Figures 1B,C ). Here, the NICD binds directly to the DNA, physically moving corepressors and histones, recruiting coactivators, and activating gene transcription ( 2 , 4 , 6 ) ( Figure 1D ). (frontiersin.org)
  • Join this webinar to learn more about the alteration of host gene expression as a result of COVID-19 infection, and novel laboratory techniques designed to evaluate this at a single-cell resolution. (bucher.ch)
  • Ecker shares ,"We think it's pretty striking that we can tease apart a brain into individual cells, sequence their methylomes, and identify many new cell types along with their gene regulatory elements, the genetic switches that make these neurons distinct from each other. (epigenie.com)
  • Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
  • The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
  • whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)
  • However, in HTC-treated cells, lysosome density increased and mitochondrial membranes modified indicating a decline in the respiratory activity. (biosafety-info.net)
  • CFSE is also an ester compound that passes through viable cell membranes. (dojindo.com)
  • We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
  • A/LCI uses light scattering to detect changes in the size of cell nuclei, which indicates dysplasia, or precancerous tissues, in the esophageal epithelium," says Adam Wax, professor of biomedical engineering at Duke University's Pratt School of Engineering (Durham, NC). (mddionline.com)
  • It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy. (sigmaaldrich.com)
  • Every cell line was supplied by ATCC with culture reagents sourced from Life Technologies. (news-medical.net)
  • S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. (bucher.ch)
  • A robust and accurate algorithm to acquire the 3D positions of the cells would help to reveal the mechanisms of embryogenesis. (nature.com)
  • This in vitro platform can be used to study how individual cells respond to physically constrained spaces and may provide insights into tip-growth mechanisms. (jove.com)
  • Asymmetric cell divisions (ACDs) generate two daughter cells with identical genetic information but distinct cell fates through epigenetic mechanisms. (elifesciences.org)
  • Both extrinsic and intrinsic mechanisms determine distinct daughter cell fates after ACD. (elifesciences.org)
  • Caenorhabditis elegans represents a valuable model for investigating ACD, given its invariant cell lineage and conserved mechanisms of ACD. (elifesciences.org)
  • Although IL-22-producing helper T (Th22) cells have been reported to be involved in human tuberculosis, the mechanisms by which Th22 cells differentiate and are recruited into the pleural space are unknown. (atsjournals.org)
  • Despite recent advances, the mechanisms of RNA movements and targeting within the nucleus are still mysterious. (cnrs.fr)
  • Considerable research has been done into preparations that simulate immune mechanisms, in particular non-specific cell-mediated and humoral immunity, in the youngest animals [1,2,3,4,5]. (researchsquare.com)
  • To analyse the time-series 3D microscopic images of developing embryos with fluorescently labelled nuclei, these studies used image segmentation. (nature.com)
  • The two other, rather new but more reliable approaches are either by detecting areas of different probe mobility on an individual image basis or by physical modeling of fluorescence loss from moving bodies. (wikipedia.org)
  • One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)
  • VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
  • One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked, structure that emits red fluorescence with the maximum wavelength at 650 nm. (dojindo.com)
  • Mice deficient in Notch3 have defects in arterial vascular smooth muscle cell (VSMC) mechanosensitivity, including impaired myogenic responses and autoregulation, and inappropriate VMSC orientation. (cdc.gov)
  • This condition is defined by the finding of a hypoplastic bone marrow that has fatty replacement and that may have relatively increased nonhematopoietic elements, such as mast cells. (medscape.com)
  • Bone marrow aspirate demonstrating plasma cells of multiple myeloma. (medscape.com)
  • Spinning Disk Confocal Microscopy - Spinning disk microscopy has advanced significantly in the past decade and now represents one of the optimum solutions for both routine and high-performance live-cell imaging applications. (fsu.edu)
  • It was found that methane significantly prolonged rat survival, decreased the W/D ratio, alleviated LPS-induced histological changes, and reduced apoptotic cells in the testes. (hindawi.com)
  • It is noteworthy that 105 of the 131 apoptotic cells (Q.aa and Q.pp), respectively ( Figure 1A ). (elifesciences.org)
  • QL or QR neuroblast each generates three neurons and two apoptotic cells (Q.aa/Q.pp, X). QL produces PQR, PVM, and SDQL. (elifesciences.org)