ImmunohistochemistryMicroscopyPolymerase chain reRRNA-targeted oligonucleotide probesBrighter fluorescenceFISHImmunophenotypingFluorescentDetectionExcitationProbesFRETTelomericAssayQuantitativeMethodsHiLyteFluoresceinVivoAnalysisMolecularProbeBoneTissueCytometerCellsBiomarkersCellTestDiagnosisOptimalTestsSamplesAgreement
Immunohistochemistry2
- 13. Cell Type-Specific Quantification of Telomere Length and DNA Double-strand Breaks in Individual Lung Cells by Fluorescence In Situ Hybridization and Fluorescent Immunohistochemistry. (nih.gov)
- In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)
Microscopy4
- The diversification of the original FISH protocol into the impressive number of procedures available these days has been promoted throughout the years by a number of interconnected factors: the improvement in sensitivity, specificity and resolution, together with the advances in the fields of fluorescence microscopy and digital imaging, and the growing availability of genomic and bioinformatic resources. (biosyn.com)
- Probes and targets are finally visualized in situ by microscopy analysis. (biosyn.com)
- This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc. (bdbiosciences.com)
- Today, with the advent of superresolution fluorescence microscopy recognized by the 2014 Nobel Prize in Chemistry, cellular architecture and function can be studied with outstanding spatial resolution down to 10 nm. (aip.org)
Polymerase chain re1
- These have included improvements in the sensitivity of routine radiologic techniques such as computed tomography (CT) scanning, and the development of highly sensitive methods for detecting very low levels of lymphomatous involvement of some tissues, including the use of flow cytometry, molecular methods such as polymerase chain reaction (PCR), and fluorescence in situ hybridization (FISH). (cancernetwork.com)
RRNA-targeted oligonucleotide probes3
- Another useful technique, Fluorescence in situ hybridization (FISH) uses rRNA-targeted oligonucleotide probes to identify individual microbial cells in situ. (assignmenthippo.com)
- In microbial ecology, Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes tool is highly used (10). (assignmenthippo.com)
- Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. (springer.com)
Brighter fluorescence2
- DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence. (vectorlabs.com)
- HiLyte™ Fluor 488 is a green flurophore with brighter fluorescence and better photostability than fluorescein, such as FITC. (eurogentec.com)
FISH17
- Metaphase cells and interphase nuclei derived from a routine unstimulated culture of bone marrow were analyzed using a combined strategy of G-banding and fluorescent in situ hybridization (FISH) in addition to hematopathological analysis, flow cytometry, and molecular genetics. (nih.gov)
- 4. Flow cytometric analysis of fluorescence in situ hybridization with dye dilution and DNA staining (flow-FISH-DDD) to determine telomere length dynamics in proliferating cells. (nih.gov)
- 8. Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans. (nih.gov)
- 12. Telomere length analysis by quantitative fluorescent in situ hybridization (Q-FISH). (nih.gov)
- The introduction of FISH (fluorescence in situ hybridization) marked the beginning of a new era for the study of chromosome structure and function. (biosyn.com)
- We present the cytogenetic, interphase fluorescence in-situ hybridization (FISH) and DNA content findings in a clinically aggressive adenoid cystic carcinoma (ADCC) of the parotid gland. (nih.gov)
- The tumor manifested diploid chromosomal and DNA content by cytogenetic, interphase FISH and flow cytometry. (nih.gov)
- Additionally, we read fluorescence in-situ hybridization (FISH) tests in-house. (southcentralpathology.com)
- For example, endoscopy, 1 flow cytometry, 2 and fluorescence in situ hybridization (FISH) 3 are routinely used for early detection and prognosis of cancer (e.g., breast cancer, stomach cancer, and leukemia), while optical coherence tomography (OCT) 4 and laser-assisted in situ keratomileusis (LASIK) are powerful diagnostic and surgical tools in ophthalmology, respectively. (aip.org)
- DNA copy number was analyzed by performing fluorescence in situ hybridization (FISH). (biomedcentral.com)
- The use of oligonucleotide in FISH is beneficial because of optimal experimental conditions which give good fluorescence signal to microbial cells containing rRNA probes. (assignmenthippo.com)
- The prokaryotic communities of four salterns (Bingöl, Fadlum, Kemah, and Tuzlagözü) in Turkey were examined and compared using the cultivation and cultivation-independent methods [fluorescence in situ hybridization (FISH) and 454 pyrosequencing]. (springer.com)
- Direct comparison of flow-FISH and qPCR as diagnostic tests for telomere length measurement in humans. (telomerescience.com)
- Commonly used methods are terminal restriction fragment (TRF) analysis by Southern blot, fluorescence in situ hybridization coupled with flow cytometry (flow-FISH), and quantitative PCR (qPCR). (telomerescience.com)
- Here, we directly compared the performance of flow-FISH and qPCR to measure leukocytes' telomere length of healthy individuals and patients evaluated for telomeropathies, using TRF as standard. (telomerescience.com)
- There are other informative tests, such as the comprehensive cytogenetics, which is often sent off of the bone marrow aspirate, as well as the FISH [fluorescence in situ hybridization] test to look for cytogenetic changes. (targetedonc.com)
- Une quantification par cytométrie en flux trois couleurs des cellules CD34+/Annexine+/PI, une analyse FISH, un marquage immunocytochimique des préparations cytospines de la moelle osseuse et des cultures de cellules souches à long terme ont été réalisés. (who.int)
Immunophenotyping1
- Flow cytometry immunophenotyping using a large panel of antibodies is needed as well as confirmatory immunohistochemical analysis and cytochemistry studies for myeloperoxidase and nonspecific esterase. (medscape.com)
Fluorescent5
- 1. γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue. (nih.gov)
- These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, In-situ Hybridization, or flow cytometry. (2bscientific.com)
- Flow cytometry and cell sorting, when combined with the application of fluorescent probes, have the capability for the detection and separation of diverse microbial populations from within complex mixtures. (edu.au)
- We describe here the combination of fluorescent in situ hybridization and cell sorting for the detection and isolation of Candidate Division TM7 bacteria from an enriched soil sample. (edu.au)
- Yn dizze test wurdt it stekproef ferwurke en antykladen dy't tagged binne mei fluorescent markers wurde tafoege. (lymphoma.org.au)
Detection5
- Detection of unexpected clones of monosomy 7 in childhood acute lymphoblastic leukemia using fluorescence in situ hybridization. (nih.gov)
- 18. Improved in situ detection method for telomeric tandem repeats in metaphase spreads and interphase nuclei. (nih.gov)
- Furthermore, high-throughput screening based on multiparameter fluorescence detection plays a critical role in drug discovery. (aip.org)
- However, with the advent of new molecular technologies like PCR sequencing and hybridization techniques, sensitive detection of bacteria has now become possible (2). (assignmenthippo.com)
- Integrated Genomic DNA/RNA Profiling vs Fluorescence in Situ Hybridization in the Detection of MYC and BCL2 (and BCL6) Rearrangements in Large B-Cell Lymphomas: Updates Amid the New WHO Classification of Lymphoid Neoplasms. (cdc.gov)
Excitation2
- The fluorescence signal of HiLyte™ Fluor 750-conjugate can be observed at 778 nm with the excitation at 754 nm. (anaspec.com)
- The fluorescence of HiLyte™ Fluor 488 can be observed at excitation/emission wavelength of 495 nm/524 nm. (eurogentec.com)
Probes5
- 14. Detecting RNA/DNA hybridization using double-labeled donor probes with enhanced fluorescence resonance energy transfer signals. (nih.gov)
- Helper nucleotide facilitates improved in situ hybridization of six probes to three different helices (7). (assignmenthippo.com)
- One study on 16S rRNA of E.coli showed that about 20% signal of the maximal fluorescence signal is received in one third of 200 probes examined (7). (assignmenthippo.com)
- Apart from this, PNA probes have high efficiency of hybridization, excellent binding capacity and competent enough for target cell enumeration. (assignmenthippo.com)
- Amann RI (1995) In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. (springer.com)
FRET4
- 3. A practical approach to FRET-based PNA fluorescence in situ hybridization. (nih.gov)
- 7. Ultrasensitive FRET-based DNA sensor using PNA/DNA hybridization. (nih.gov)
- 16. Hybridization accompanying FRET event in labeled natural nucleoside-unnatural nucleoside containing chimeric DNA duplexes. (nih.gov)
- 19. A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization. (nih.gov)
Telomeric1
- The modern technology uses fluorescein-labeled oligonucleotides to monitor in situ hybridization to telomeric repeat sequence (12). (assignmenthippo.com)
Assay1
- 17. Dual labeled fluorescence probe based qPCR assay to measure the telomere length. (nih.gov)
Quantitative1
- p16 flow cytometry of exfoliated cervical cells: Its role in quantitative pathology and clinical diagnosis of squamous intraepithelial lesions. (cdc.gov)
Methods1
- Eckford-Soper, LK , Davidson, K & Bresnan, E 2013, ' Identification and quantification of toxic and nontoxic strains of the harmful dinoflagellate Alexandrium tamarense using fluorescence in situ hybridization and flow cytometry ', Limnology and Oceanography - Methods , vol. 11, pp. 540-548. (uhi.ac.uk)
HiLyte1
- Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647 or allophycocyanin (APC), facilitating multicolor analysis. (anaspec.com)
Fluorescein1
- 11. Fluorescence energy transfer between fluorescein label and DNA intercalators to detect nucleic acids hybridization in homogeneous media. (nih.gov)
Vivo2
- 20. Visualizing nucleic acids in living cells by fluorescence in vivo hybridization. (nih.gov)
- By performing two-photon in vivo imaging and flow cytometry analyses, we showed that microglia first exhibit Aβ chemotaxis (3-12 h after injection) and subsequently phagocytose Aβ (15-24 h after injection) in APP/PS1 mice, a mouse model of amyloidosis 23 . (nature.com)
Analysis4
- 2. Cooperative hybridization of γPNA miniprobes to a repeating sequence motif and application to telomere analysis. (nih.gov)
- 9. Telomere analysis by fluorescence in situ hybridization and flow cytometry. (nih.gov)
- Antón J, Llobet Brossa E, Rodríguez-Valera F, Amann R (1999) Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds. (springer.com)
- While the proposed studies focus on the use of our nanopipette technology to study human cancer cells in culture, the cell monitoring capabilities of the nanopipette allow it to be used for in situ analysis of single cells in tissue biopsies and slices. (nih.gov)
Molecular2
- Now, for patients who can't be assessed molecularly because there's no molecular lesion at the time of their original diagnosis, you can also use flow cytometry. (medscape.com)
- Mammalian cell biology is dynamic, but today's 'omic approaches require destruction of cells to access their molecular contents and cannot resolve the full richness of cellular diversity in situ . (nih.gov)
Probe2
- 5. Measurement of telomere length using PNA probe by cytometry. (nih.gov)
- Dûnse plakjes fan 'e biopsie-probe (of tinne floeistoflagen) wurde behannele mei sets fan antykladen dy't ferskillende markers werkenne fûn yn ferskate soarten lymfomen of leukemyske sellen en normale lymphozyten. (lymphoma.org.au)
Bone1
- The bone marrow biopsy and the peripheral blood can be evaluated using a flow cytometry test to look at the surface markers on the cancer cells or the blast cells. (targetedonc.com)
Tissue1
- Tissue-foarbylden kin bloedûndersiken en weefselbiopsies omfetsje. (lymphoma.org.au)
Cytometer3
- An instrument known as flow cytometer is used to measure the number and percentage of cells and their characteristics. (onco.com)
- Ingxube ye-cell-antibody ijovwa entweni ebizwa ngokuthi i-flow cytometer. (lymphoma.org.au)
- Musanganiswa we cell-antibody unobaiwa muchiridzwa chinonzi flow cytometer. (lymphoma.org.au)
Cells1
- If CLL is present, flow cytometry may also help analyze the aggressiveness of the leukemia cells. (shebaonline.org)
Biomarkers3
- Flowcytometry mjit it oantal en persintaazje sellen yn in bloedmonster en selkenmerken lykas grutte, foarm en de oanwêzigens fan biomarkers op it sel oerflak. (lymphoma.org.au)
- Ntws cytometry ntsuas tus naj npawb thiab feem pua ntawm cov hlwb hauv cov qauv ntshav thiab cov yam ntxwv ntawm tes xws li qhov loj, cov duab thiab muaj cov biomarkers ntawm lub xov tooj ntawm tes. (lymphoma.org.au)
- Kahe cytometry ana i ka helu a me ka pākēneka o nā pūnaewele i loko o kahi hāpana koko a me nā hiʻohiʻona pūnaewele e like me ka nui, ke ʻano a me ka loaʻa ʻana o nā biomarkers ma ka ʻili o ke kelepona. (lymphoma.org.au)
Cell3
- Ovarian cancer cell DNA ploidy was determined using Feulgen-stained smears or flow cytometry. (biomedcentral.com)
- Kuyerera cytometry inoyera nhamba uye muzana yemasero muropa sampuli uye sero maitiro senge saizi, chimiro uye kuvapo kwemabiomarker pane cell cell. (lymphoma.org.au)
- Daloy ng cytometry sinusukat ang bilang at porsyento ng mga cell sa isang sample ng dugo at mga katangian ng cell tulad ng laki, hugis at pagkakaroon ng mga biomarker sa ibabaw ng cell. (lymphoma.org.au)
Test1
- Dizze test kin helpe by it diagnostearjen en identifisearjen fan spesifike soarten lymphoma. (lymphoma.org.au)
Diagnosis1
- There was an agreement that diagnosis of MPAL can be challenging, especially if applied flow cytometry panels are not comprehensive enough. (medscape.com)
Optimal1
- Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining. (vectorlabs.com)
Tests6
- We perform more specialty tests, including breast cancer prognostic markers, flow cytometry, and have a wide selection of special stains. (southcentralpathology.com)
- Diagnostyske tests wurde dien yn it patologylaboratoarium troch spesjalistyske dokters en wittenskippers. (lymphoma.org.au)
- D'r binne spesjalistyske dokters dy't spesjale training hawwe ûndergien yn dit gebiet om dizze tests te ynterpretearjen fan bloed, lymfeklieren en bonkenmurgmonsters om sykte te identifisearjen. (lymphoma.org.au)
- As wittenskippers mear begripe oer lymfoom, wurde nije en gefoeliger tests makke om dokters te ynformearjen oer de diagnoaze. (lymphoma.org.au)
- It wurdt wichtiger dat dizze tests wurde útfierd sadat se it type en gedrach fan it lymfoom begripe foardat se beslute oer de juste behanneling foar de pasjint. (lymphoma.org.au)
- Se sille ekstra tests dwaan lykas immunofenotyping, cytogenetyske analyze en/of molekulêre stúdzjes om mear ynformaasje te finen om oan te jaan hoe't it lymfoom him kin gedrage. (lymphoma.org.au)
Samples1
- Se sjogge ek nei har foarm, har grutte en hoe't se groepeare binne yn samples fan lymfeklieren en bonken, troch ûnder de mikroskoop te sjen. (lymphoma.org.au)
Agreement1
- In the United States, in particular, there is no standardized agreement on how to follow patients with AML using flow cytometry. (medscape.com)