• Oligonucleotides are most widely used as PCR primers. (researchandmarkets.com)
  • This can be attributed to the large revenue share of oligonucleotide synthesis for production of PCR primers. (researchandmarkets.com)
  • Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)
  • Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)
  • The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
  • Save time and money with ValuMix assays, which consist of probe and primers mixed in a single tube. (biosearchtech.com)
  • Cy3 plays a particularly important role in real-time PCR applications, being used as the reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). (genelink.com)
  • Dual-Labelled BHQ Probes - Whether you require labeled, unlabeled oligos or PCR primers, LGC Biosearch Technologies can meet your exact needs as they offer custom modifications at extremely competitive rates. (2bscientific.com)
  • Strong, specific hybridization to the probe was observed for all V. anguillarum strains tested. (diva-portal.org)
  • It was even possible to detect V. anguillarum, by slot-blot hybridization, directly in a homogenized kidney from a fish that had died of vibriosis. (diva-portal.org)
  • Fluorescence in-situ hybridization (FISH) was subsequently tested in conjunction with a range of oligonucleotide probes for the detection of legionella in environmental samples. (dguv.de)
  • Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
  • Cytogenetic, fluorescence in situ hybridization (FISH), and loss-of-heterozygosity (LOH) studies have revealed that MRTs frequently contain deletions at chromosome locus 22q11.1. (medscape.com)
  • Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
  • The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
  • Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
  • Cy3 can also be used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH). (genelink.com)
  • This Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH 0 strategy may provide an effective solution to the problem of low signal intensity, which is commonly observed when using corresponding singly-labeled FISH probes for microbe identification. (genelink.com)
  • Among them, Fluorescent in situ hybridization (FISH) is rapid, sensitive and quantitative. (granthaalayahpublication.org)
  • Fluorescently labeled oligonucleotide probes were used for hybridization. (granthaalayahpublication.org)
  • Oligonucleotide probes are increasingly being used to characterize natural microbial assemblages by Tyramide Signal Amplification-Fluorescent in situ Hybridization (TSA-FISH, or CAtalysed Reporter Deposition CARD-FISH). (hal.science)
  • The probes were confronted to recent databases and hybridization conditions were tested against target strains matching perfectly with the probes, and against the closest non-target strains presenting one to four mismatches. (hal.science)
  • We increased the hybridization stringency from 55 to 65% formamide for the Eub338+EubII+EubIII probe mix to be specific to the Eubacteria domain. (hal.science)
  • The technology also increases the density of genomic DNA probes for higher resolution genetic analysis beyond what is used in FISH, genomic microarrays for array comparative genomic hybridization, and solution capture hybrdization arrays for sequence enrichment in deep sequencing. (cytognomix.com)
  • Oligonucleotides fluorescence in situ hybridization (Oligo-FISH) is an rising know-how and is a crucial device in analysis areas comparable to detection of chromosome variation, identification of allopolyploid, and deciphering of three-dimensional (3D) genome constructions. (iba-biotagnology.com)
  • Metaphase chromosome spreads were prepared for quantitative fluorescence in situ hybridization (Q-FISH) analysis using oligonucleotide N3'→P5'phosphoramidate probes specific for telomere DNA sequences (green color), centromeric DNA sequences (red color) probe, and the general DNA dye DAPI (blue color). (utsouthwestern.edu)
  • Oligomers are produced synthetically through solid-phase chemical synthesis for use in PCR (polymerase chain reaction, where the oligosaccharide used is referred to as the primer ), DNA sequencing, DNA microarrays, Southern blots, ASO analysis, FISH (florescent in situ hybridization), molecular probing, and library construction. (biologyonline.com)
  • Short-chain fatty-acids (SCFAs) produced from in vitro fermentation were measured using HPLC and bacterial populations were measured using flow cytometry with fluorescence in situ hybridisation (Flow-FISH). (springeropen.com)
  • Take advantage of our expertise and experience to build high quality research-grade oligos to your exact specifications with our large selection of probe chemistries, modifications, purification options and manufacturing scales. (biosearchtech.com)
  • Visit the LNA Probes product page to download one of four excel order forms for LNA oligos in a single tube or as a ValuMix assay. (biosearchtech.com)
  • Pinpoint FISH is a fully synthetic FISH technology based on single stranded fluorescently labeled DNA oligonucleotides. (biocat.com)
  • It is available as both a phosphoramidite and an NHS ester, and is used to fluorescently label oligonucleotides at either the 5' or 3' end, or internally. (genelink.com)
  • Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts. (bioscience.co.uk)
  • Try multiple probe types early in assay development and select the type that performs best with your specific application and oligo sequence. (biosearchtech.com)
  • The Global Oligonucleotide Synthesis Market Report offers this critical insight to enhance your business acumen and expedite strategic decision-making. (researchandmarkets.com)
  • It provides comprehensive market analysis, highlighting the escalating use of oligonucleotide synthesis in diverse fields ranging from human health management to molecular diagnostics and therapeutics. (researchandmarkets.com)
  • It delves into the growing demand for cancer-related therapies and how this has fueled the exploration of custom oligonucleotide synthesis in cancer research. (researchandmarkets.com)
  • Make informed, strategic decisions that will strengthen your presence in the oligonucleotide synthesis market. (researchandmarkets.com)
  • The global oligonucleotide synthesis market size is expected to reach USD 8.83 billion by 2030. (researchandmarkets.com)
  • The increasing usage of oligonucleotide synthesis in several field such as human health management, molecular diagnostics, and therapeutics have boosted the demand for this product thereby contributing to the market's revenue growth. (researchandmarkets.com)
  • Moreover, this has resulted in an increase in investments aiding to explore the potential application of custom oligonucleotide synthesis in cancer research. (researchandmarkets.com)
  • By product type, the reagents segment is anticipated to witness significant growth in the coming years as reagents have recurring demand and usage in the oligonucleotide synthesis process. (researchandmarkets.com)
  • Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
  • PCR is an excellent method for probe synthesis, requiring very small quantities of template material. (enzolifesciences.com)
  • If the probe set has not yet been designed, contact us with the target sequences (accession number and description of the target of interest) . (bioscience.co.uk)
  • US Patent 8,209,129 has issued on single copy DNA probes which include divergent repetitive sequences, thus significantly extending the portions of the genome that can be used for such probes beyond traditional single copy sequences. (cytognomix.com)
  • In 2010, Stoeckler and co-workers (4) reported that Cy3 double-labeling of FISH probes (at both ends) that were specific to ribsosomal RNA targets in microorganisms at least doubles FISH signal intensity without affecting specificity. (genelink.com)
  • In view of the fast-growing rRNA databases, we re-evaluated the in silico specificity of eleven bacterial and eukaryotic probes and competitor frequently used for the quantification of marine picoplankton. (hal.science)
  • In addition, we found that recent changes in the Gammaproteobacteria classification decreased the specificity of Gam42a probe, and that the Roseo536R and Ros537 probes were not specific to, and missed part of the Roseobacter clade. (hal.science)
  • Experiences acquired through these investigations leads us to propose the use of seven steps protocol for complete FISH probe specificity checkup to improve data quality in environmental studies. (hal.science)
  • From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification of V. anguillarum. (diva-portal.org)
  • Combined analyses including FISH, coding sequence analysis, high-density single nucleotide polymorphism-based oligonucleotide arrays, and multiplex ligation-dependent probe amplification enable the identification of biallelic, inactivating perturbations of SMARCB1 in nearly all MRTs, consistent with the 2-hit model of tumor formation. (medscape.com)
  • Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
  • In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)
  • In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
  • A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
  • Aptamers (from the Latin aptus ("- fit") and Greek meros ("-part") that are oligonucleotides (since other aptamers can be peptide molecules) are created usually from a large random sequence pool and used mainly for research and clinical applications (e.g. as macromolecular drugs). (biologyonline.com)
  • In molecular genetics, they refer to a short sequence synthesised to match a regionwhere a mutation is known to occur, and then used as a probe (oligonucleotide probes). (biologyonline.com)
  • Different types of probes may investigate a broad range of sizes of DNA sequence. (msdmanuals.com)
  • The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. (bioscience.co.uk)
  • NB: Due to variable auto-fluorescence in the green channel inherent to cells and tissue, it is not recommended to use the FAM (fluorescein) for some Stellaris RNA FISH applications. (bioscience.co.uk)
  • An online account also provides you free access to various design software such as RealTimeDesign™ Software, Stellaris® Probe Designer, and ChIRP Designer. (biosearchtech.com)
  • Stellaris FISH RNA Probes - Stellaris® RNA FISH is a RNA visualization method that allows simultaneous detection, localisation and quantification of individual mRNA molecules at the cellular level in fixed samples. (2bscientific.com)
  • Expand beyond standard qPCR probe chemistries and take advantage of our versatile suite of oligo options to get exactly what you need. (biosearchtech.com)
  • We show that FISH not only detect active WD but also be helpful as an indicator for the efficiency of antibiotic treatment and for detection of recurrence of disease when the signal of PAS diastase and immunohistochemistry lags behind the recurrence of disease, especially if the clinical course of the patient and antimicrobial treatment is considered. (frontiersin.org)
  • FISH is a useful detection tool in culture-negative cases with tissue lesions suggestive of bacterial infection. (dtu.dk)
  • Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. (bioscience.co.uk)
  • Besides premade probes, also genome wide, custom Pinpoint FISH assays engineered to meet your specific requirements are offered. (biocat.com)
  • DNA from a patient is compared to a reference genome using many oligonucleotide probes. (msdmanuals.com)
  • species-specific oligonucleotide probes directed against either C. fetus or C. jejuni were used in combination with a general bacterial probe. (dtu.dk)
  • This set of oligonucleotides included a genus-specific probe for Methylocystis (Mcyst-1432) and three species-specific probes for Methylosinus sporium (Msins-647), Methylosinus trichosporium (Msint-1268) and the recently described acidophilic methanotroph Methylocapsa acidiphila (Mcaps-1032). (mpg.de)
  • Gene probes can be used to locate specific segments of normal or mutated DNA. (msdmanuals.com)
  • Gene probes can detect a number of disorders before and after birth. (msdmanuals.com)
  • Exploring the molecular world, the need for custom design and manufacture of synthetic oligonucleotides continues to expand at an increasingly faster pace. (2bscientific.com)
  • As simultaneously identified and typed all component strains an alterative, high-density oligonucleotide resequencing of the trivalent FluMist intranasal vaccine. (cdc.gov)
  • Centromere Enumeration Probes (sub-CEPs) from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
  • The Pinpoint FISH TP53/CEP 17 Probe Kit is intended to detect the copy number of the LSI TP53 probe target located at chromosome 17q11.1 and of the CEP 17 probe target located at the centromere of chromosome 17. (biocat.com)
  • We have a long-standing collaboration with Wolfgang Ludwig and Ralf Westram, the main developers of the ARB program that is widely used for the reconstruction of 16S rRNA-based phylogenetic trees and for the design of oligonucleotide probes. (mpi-bremen.de)
  • Mcyst-1432), and with an additional probe (Mcyst-1261) that had been designed to target a defined phylogenetic subgroup of Methylocystis spp. (mpg.de)
  • One of the benefits of working with Biosearch Technologies is our extensive probe technology menu. (biosearchtech.com)
  • In late spring, 15 ± 2% of cells hybridised with a probe specific to the Kingdom Archaea. (soton.ac.uk)
  • The majority of cells (88 ± 2%) belonged to the Kingdom Bacteria, and 86% of cells were identified using group-specific probes. (soton.ac.uk)
  • The largest detectable population of indigenous methanotrophs simultaneously hybridised with a group-specific probe targeting all currently known Methylosinus/Methylocystis spp. (mpg.de)
  • M-450), with a genus-specific probe for Methylocystis spp. (mpg.de)
  • Furthermore, FISH is a fast and economical method to detect and identify the zoonotic agent Campylobacter within ovine abortion material. (dtu.dk)
  • Using custom probe sets enables the researcher to design their own to detect the RNA of interest. (bioscience.co.uk)
  • In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
  • Aerobic cellulose degrading Cytophaga bacteria have been identified on fishing nets made from cotton or hemp used by Japanese fishermen. (wikipedia.org)
  • DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
  • Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
  • Nucleotides may be grouped into mononucleotide s, dinucleotide s, oligonucleotide s, and polynucleotide s. (biologyonline.com)
  • An oligonucleotide is a compound comprised of about three to twenty nucleotides. (biologyonline.com)
  • This study has demonstrated the ability of otolith chemistry to discriminate source populations of pelagic larvae in a fully marine environment, which provides a basis to quantify larval movement between fish populations. (frontiersin.org)
  • The elemental composition of microscopic otoliths of fish larvae were used as an environmental marker to differentiate between populations of larvae. (frontiersin.org)
  • 2017). Single cell genomics and/or metagenome-assembled genomes, often combined with single cell identification by FISH, discloses the true diversity of microorganisms, which is breathtaking, yet certainly not endless (Amann & Rossello-Mora 2016). (mpi-bremen.de)
  • Whole Chromosome Pinpoint FISH Paints from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
  • KromaTiD Whole Chromosome Pinpoint FISH Paints make use of non-repetitive genomic DNA to provide a uniform signal from chromosome to chromosome. (biocat.com)
  • A subset of probes was mapped onto chromosome topography by FISH-correlated atomic force microscopy (AFM). (cytognomix.com)
  • Additional non-DA probes ( NOMO1 , NOMO3 ) hybridized to grooves in chromosome topography and exhibited a narrow range of probe depths (average: 0.08 μm) along axial and lateral axes of the 2 homologs. (cytognomix.com)
  • Pinpoint FISH will run on standard cytogenetic systems, requires no special equipment, and produces data indistinguishable from BAC FISH. (biocat.com)
  • We report molecular cytogenetic data showing local differences in condensation between homologs that are related to differences in accessibility (DA) of associated DNA probe targets. (cytognomix.com)
  • Whereas the transfer of DA from planktivorous fish to piscivorous predators has been well established, the relationship between toxin levels in plankton-feeding fish and the regional abundance of DA-producing diatoms has not been documented. (researchgate.net)
  • For this purpose, a number of fluorescence-based methods, such as the use of genetic probes and a range of dyes, were to be tested on laboratory and industrial samples. (dguv.de)
  • This report also emphasizes the preference towards oligonucleotides by pharmaceutical and biotechnology companies due to their cost-effectiveness and rational drug design. (researchandmarkets.com)
  • As an added benefit, Cy3-doubly labeled probes were shown to increase the in situ accessibility of rRNA targets sites in microbes, which allows for greater probe design flexibility. (genelink.com)
  • Fish contained DA levels above the regulatory limit (20 g DA g-1 whole fish) only when toxic cell densities exceeded 104 cells l-1. (researchgate.net)
  • To quantify DA and pinpoint probe locations, we performed 3D-structured illumination super-resolution microscopy (3D-SIM). (cytognomix.com)
  • The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation. (bioscience.co.uk)
  • Fourteen short (1.5-5kb) sc and low copy (lc) FISH probes (from chromosomes 1, 5, 9, 11, 15, 16, 17, 22) targeting genic and non-genic regions with and without DA were developed and hybridized to cells from 10 individuals with cytogenetically-distinguishable homologs. (cytognomix.com)
  • Standard Pinpoint FISH DNA Probes are available with 5 different fluorophores. (biocat.com)
  • Telomeric Probes (sub-Telos) from KromaTiD are available for the p- and q-arms of all human chromosomes as standard products, other sequenced species are available by request. (biocat.com)
  • We confirmed the eukaryotic probes original conditions, and propose the Euk1209+NChlo01+Chlo02 probe mix to target the largest picoeukaryotic diversity. (hal.science)
  • DA was detected in fish viscera samples whenever toxic diatom densities reached 䒟 cells l-1 in surface waters, suggesting that anchovies and sardines regularly consume toxic diatoms when present. (researchgate.net)
  • Maximum DA levels detected in fish were 1,815 g DA g-1 in anchovy and 728 g DA g-1 in sardine viscera samples. (researchgate.net)
  • Access the online ordering tool for the probe type via the 'Order now' tab on the product page. (biosearchtech.com)