Eukaryotic saccharomyces cerevisiae proteins. Medical search. Frequent questions

Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
Alkalinization of the medium represents a stress condition for the budding yeast Saccharomyces cerevisiae to which this organism responds with profound remodeling of gene expression. (microbialcell.com)
The budding yeast Saccharomyces cerevisiae grows under a relatively broad range of external pH, but proliferates far better at acidic than at neutral or alkaline pH. (microbialcell.com)
now show that this is not the case for budding yeast ( Saccharomyces cerevisiae ). (elifesciences.org)
The work in her laboratory, the Huang Lab, seeks to understand the complex morphological changes that occur during sporulation in the budding yeast, Saccharomyces cerevisiae . (umb.edu)
To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
Much of our work is conducted in the yeast Saccharomyces cerevisiae, an organism that permits both facile genetic manipulation and detailed biochemical analysis. (yale.edu)
We performed mutagenesis to introduce changes equivalent to 12 human CACH/VWM mutations in three subunits of the equivalent factor from yeast (Saccharomyces cerevisiae) and analyzed effects on cell growth, translation, and gene expression in response to stresses. (yeastgenome.org)
To understand the molecular biology of peptide hormone action, we studied response of budding yeast ( Saccharomyces cerevisiae ) to its peptide mating pheromones ( a -factor and α-factor). (berkeley.edu)
We used cryo-EM to investigate the nucleotide-driven reaction cycle of condensin from the budding yeast Saccharomyces cerevisiae . (nature.com)
The budding yeast Saccharomyces cerevisiae was used as eukaryotic reference cell to investigate the impact of αSyn on protein homeostasis and the interplay between αSyn and proteins associated with 26S proteasome. (uni-goettingen.de)

This gene encodes a protein that is homologous to Maf1, a Saccharomyces cerevisiae protein which is highly conserved in eukaryotic cells. (wikipedia.org)
This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. (nih.gov)

In this chapter, we will discuss the principle of heterologous gene expression, different types of hosts organism available for heterologous protein production. (benthamscience.com)
Although most of the work was done on budding yeast, because of the relative ease of genetic and biochemical studies in this organism, it is safe to assume that most of the observations also apply to higher eukaryotes, as nearly all the proteins involved in ribosome assembly are conserved between yeast and human. (biomedcentral.com)
Saccharomyces cerevisiae is a widely used model organism in science, and therefore also one of the most studied (along with E. coli ). (wikidoc.org)
Genes are regions of a genome encoding proteins of an organism. (edu.vn)
We are using the yeast model organism Saccharomyces castellii, which has telomere traits and telomerase activity that are highly similar to human cells. (lu.se)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)

For example, transcript elongation factor TFIIS is highly conserved among eukaryotes, and yet the TFIIS protein from Saccharomyces cerevisiae cannot function with mammalian RNA polymerase II and vice versa. (embl.de)
Different types of hosts are commonly used for heterologous protein production include Escherichia coli, Saccharomyces cerevisiae, insect cell expression systems and various mammalian systems. (benthamscience.com)
A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
Unlike yeast, most mammalian ribosomal proteins are thought to be encoded by a single gene copy, raising the possibility that heterogenous populations of ribosomes are unique to yeast. (prolekarniky.cz)
Ribosome synthesis is a highly controlled process, whereby three distinct RNA polymerases are synchronously coordinated to produce equimolar amounts of four rRNAs and 79 mammalian ribosomal proteins (RPs) [1] - [4] . (prolekarniky.cz)
We use Gibco cell culture medium and related transfection reagents to ensure the high quality and high expression level of each batch of mammalian cell protein expression products. (kmdbioscience.com)
Expression system of mammalian cells and insect cells: mammalian cells expressing foreign recombinant proteins can be transfected with plasmids and infected with viral vectors. (medicilon.com)

Based on the above-mentioned major R&D platforms, KMD Bioscience has successfully developed many related reagents such as recombinant proteins, antibodies, antibody-drug similars, industrial enzymes, and diagnostic raw materials for scientific research and new drug discovery. (kmdbioscience.com)
Medicilon researchers have established a mature E. coli expression and purification service platform to provide expression and purification services including various recombinant proteins and their complexes in E. coli. (medicilon.com)

Ribosomes are the cellular structures responsible for the synthesis of protein in all branches of life. (utexas.edu)
All ribosomes are made from a large and small subunit that in turn are composed of protein and RNA. (utexas.edu)
The synthesis of eukaryotic ribosomes is a complex process involving more than 200 factors and spans three cellular compartments: the nucleolus, the nucloplasm, and the cytoplasm. (utexas.edu)
Using mass spectrometric analyses of Saccharomyces cerevisiae ribosomes, we found that the amino acid residue in closest proximity to the decoding center, Pro-64 of the 40S subunit ribosomal protein Rps23p (RPS23 Pro-62 in humans) undergoes posttranslational hydroxylation. (ox.ac.uk)
In a separate line of research, proteomic studies of mature plant ribosomes have revealed considerable variability in the protein composition of individual ribosomes. (biomedcentral.com)
The synthesis of ribosomes is a major metabolic task in eukaryotic cells. (biomedcentral.com)
Although eukaryotic ribosomes are composed of only four ribosomal RNAs (rRNAs) and around 80 ribosomal proteins, many other proteins are recruited to help deliver ribosomal subunits to the cytoplasm - at the rate of 2,000 or so ribosomes each minute in a growing yeast cell, for example [ 2 , 3 ]. (biomedcentral.com)
Most yeast ribosomal protein genes are duplicated and their characterization has led to hypotheses regarding the existence of specialized ribosomes with different subunit composition or specifically-tailored functions. (prolekarniky.cz)

To determine the reason for this species specificity, chimeras were constructed linking three structurally independent regions of the TFIIS proteins from yeast and human cells. (embl.de)
This bacterial pathogen multiplies exclusively within host cells in a characteristic vacuole (inclusion) and delivers proteins such as inclusion membrane proteins (Incs) into the host cell. (bvsalud.org)
Here, we identified CT006 as a novel C. trachomatis protein that when expressed ectopically eukaryotic cells can associate with lipid droplets (LDs). (bvsalud.org)
Cytoplasmic lipid droplets are important organelles in nearly every eukaryotic and some prokaryotic cells. (bvsalud.org)
SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. (gla.ac.uk)
The excision of non-coding sequences that are present in the pre-mRNA is a hallmark of eukaryotic cells. (uni-goettingen.de)
Posttranscriptional and posttranslational modifications are key regulatory mechanisms to expand biological properties of proteins and nucleic acids in living cells. (uni-goettingen.de)
Protein synthesis is an important house-keeping activity of all the cells. (uni-goettingen.de)
4 While proteins can be phosphorylated on nine amino acids, serine, threonine and tyrosine phosphorylation are by far the most predominant in eukaryotic cells. (ac.be)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
S. cerevisiae ) (BY4741) and of human hepatocytic cells (HuH-7). (nature.com)
Saccharomyces cerevisiae cells are round to ovoid, 5-10 micrometres in diameter. (wikidoc.org)
Additionally, yeasts are comparatively similar in structure to human cells, both being eukaryotic, in contrast to the prokaryotes (bacteria and archaea). (wikidoc.org)
Consistent with the hypothesis that either ribosomal protein can support translation, knockdown of Rpl22l1 impairs growth of cells lacking Rpl22 . (prolekarniky.cz)
Modulation of chloride secretory responses and barrier function of intestinal epithelial cells by the Salmonella effector protein SigD. (microbiologyresearch.org)
More generally, we study the reversible enzymatic coupling of proteins to other proteins within cells. (yale.edu)
Our lab has as its general focus one of the fundamental regulatory systems of eukaryotic cells - the ubiquitin system. (yale.edu)
First, we wish to understand, at a mechanistic and molecular level, how specific proteins are rapidly degraded within eukaryotic cells while most proteins are spared. (yale.edu)
Protein kinase A (PKA) is an important mediator of many signal transduction pathways that occur in eukaryotic cells, and it has been implicated as a regulator of stage differentiation in Trypanosoma cruzi. (elsevierpure.com)
Protein expression refers to a molecular biology technique that uses model organisms such as bacteria, yeast, animal cells or plant cells to express foreign gene proteins. (medicilon.com)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)

To elucidate the relationship between the structure and transcription elongation function of RPB9, we initiated a mutagenesis study on the Saccharomyces cerevisiae homologue. (embl.de)
Protein synthesis can be divided into three distinct subprocesses, namely initiation, elongation and termination. (uni-goettingen.de)
E.M. Parodi, J.M. Roesner, L.S. Huang (2015) SPO73 and SPO71 function cooperatively in prospore membrane elongation during sporulation in Saccharomyces cerevisiae. (umb.edu)
2002. RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. (uni-freiburg.de)

Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
Arginine-tRNA-protein transferase catalyses the post-translational conjugation of arginine to the N terminus of a protein. (embl.de)
We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
The application of this system can be at a high level It expresses proteins and has the function of post-translational modification, so it is recognized as a powerful tool for expressing large-scale proteins. (medicilon.com)

Using the yeast structure as a template, we have modeled the homologous domains from elongin A and CRSP70 and identified a conserved positively charged patch on the surface of all three proteins, which may be involved in conserved functional interactions with the transcriptional machinery. (embl.de)
Therefore, the conversion of the DSB ends from double-stranded to single-stranded DNA, which is necessary to initiate DSB repair by homologous recombination, is responsible for loss of transcription around a DSB in S. cerevisiae . (elifesciences.org)
The proteins involved in homologous recombination have to work around other processes that go on inside the nucleus, such as the transcription of DNA in genes into RNA molecules. (elifesciences.org)
This family consists of a the C terminal region of a number of eukaryotic hypothetical proteins which are homologous to the Saccharomyces cerevisiae protein IWS1. (uni-freiburg.de)

We speculated that the defect of protein synthesis attenuated expression of other exoglucanases or glucan synthesis-related proteins, and then induced release of Xog1p to the cell wall. (ncl.edu.tw)
C. albicans Hom6p thus had an effect on cell survival and cell adhesion by maintaining protein synthesis and cell wall function. (ncl.edu.tw)
eIF4G is an essential translation factor that exerts strong control over protein synthesis. (nih.gov)
We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
Through biochemical purification of the MAP kinase Smk1, her lab identified several proteins that physically interact with Smk1, including Gsc2, a subunit of beta-glucan synthase, which plays an important role in spore wall synthesis. (umb.edu)
Given the demand for equimolar amounts of rRNA and ribosomal proteins during ribosome synthesis, it is essential that the transcription of rRNAs and of the mRNAs for ribosomal proteins is coordinated [ 1 ]. (biomedcentral.com)
Over the past few years, high-throughput experiments have provided evidence that the transcription of the auxiliary proteins involved in ribosome synthesis are also co-regulated. (biomedcentral.com)
Protein synthesis is a major energy consuming process involving intricate coordination of translation machinery in response to nutrient availability and stress sensing signals, as well as hormonal and growth factor cues in multi-cellular organisms. (prolekarniky.cz)
A growing number of human diseases have been linked to mutations in genes encoding factors involved in ribosome biogenesis and protein synthesis [5] , [6] . (prolekarniky.cz)
eIF2B is essential for protein synthesis and regulates translation in response to cellular stresses. (yeastgenome.org)
Genes are the parts of DNA that serve as a template for synthesis of proteins. (edu.vn)

Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. (lu.se)
Yeast domain II alone is able to bind yeast RNA polymerase II with the same affinity as the full-length TFIIS protein, and this domain was expected to confer the species selectivity. (embl.de)
In this review we want to give an introduction to the functional role played by ubiquitination during peroxisomal protein import and highlight the mechanistic concepts that have emerged based on data derived from different species since the discovery of the first ubiquitinated peroxin 15years ago. (ox.ac.uk)
Saccharomyces cerevisiae is a species of budding yeast . (wikidoc.org)
At the beginning of its establishment, KMD Bioscience started from the phage display technology platform to provide high-quality antibody discovery services, and gradually established stable cell line construction and screening, natural protein extraction and fermentation, and recombinant protein custom expression services, a multi-species antibody discovery platform. (kmdbioscience.com)
Increasing amounts of toxic αSyn species indicate significant perturbation of protein homeostasis in PD. (uni-goettingen.de)

Different promoter systems that are used for gene expression also influence protein production in case of both prokaryotic and eukaryotic hosts. (benthamscience.com)
This entry represents the C-terminal region of aminoacyl-transferases found in both eukaryotic (Arginine-tRNA-protein transferase) and prokaryotic (Aspartate/glutamate leucyltransferase) enzymes. (embl.de)
Prokaryotic expression system: The method of inserting the cloned gene into a suitable vector and introducing it into E. coli for expressing a large number of proteins is generally called prokaryotic expression. (medicilon.com)
General procedure for prokaryotic expression: Obtain target gene = "Preparation of expression vector = "Insert target gene into expression vector (sequencing verification) = "Transform expression host bacteria = "Induction of target protein expression = "Analysis of expressed protein = "Amplification, Purification and further testing. (medicilon.com)
Advantages: Prokaryotic protein expression system is not only the most commonly used expression system, but also the most economical protein expression system. (medicilon.com)
Yeast expression system: As an emerging foreign protein expression system, the yeast expression system is getting more and more widely used in the field of genetic engineering due to the advantages of both prokaryotic and eukaryotic expression systems. (medicilon.com)

In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
Here we show that in Saccharomyces cerevisiae a single DSB causes transcriptional inhibition of proximal genes independently of Tel1/ATM and Mec1/ATR. (elifesciences.org)
One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. (mdpi.com)
In yeast, ribosomal protein genes are generally duplicated and evidence has emerged that paralogs might have specific roles. (prolekarniky.cz)
Protein-coding and non-coding genes, splice variants, cDNA and protein sequences, non-coding RNAs. (ensembl.org)

Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
The intracellular distribution of proteins and ribonucleoproteins is a fundamental process in all eukaryotes that is critically vital for the normal functionality of the eukaryotic cell. (uni-goettingen.de)
Advantages: The yeast protein expression system is represented by Pichia pastoris, which has the advantages of high expression, inducible, glycosylation mechanism close to higher eukaryotes, easy purification of secreted proteins, and easy realization of high-density fermentation. (medicilon.com)
Palecek, J. J. & Gruber, S. Kite proteins: a superfamily of SMC/Kleisin partners conserved across bacteria, archaea, and eukaryotes. (nature.com)

S. cerevisiae has obtained this important position because of its established use in industry (e.g. beer, bread and wine fermentation, ethanol production). (wikidoc.org)

The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their cognate syntaxins. (gla.ac.uk)
The yeast SM protein Vps45p regulates membrane fusion through the trans-Golgi / late endosomal system, and interacts directly with the syntaxin (Tlg2p) and the v-SNARE (Snc2p) proteins. (gla.ac.uk)
S.M. Paulissen, C.J. Slubowski, J.M. Roesner, and L.S. Huang (2016) Timely closure of the prospore membrane requires SPS1 and SPO77 in Saccharomyces cerevisiae. (umb.edu)
E.M. Parodi, C.S. Baker, C. Tetzlaff, S. Villahermosa, and L.S. Huang (2012) SPO71 mediates prospore membrane size and maturation in Saccharomyces cerevisiae. (umb.edu)
K.L. Auld, A.L. Hitchcock, H.K. Doherty, S. Frietze, L.S. Huang, and P.A. Silver (2006) The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae. (umb.edu)
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
This ligase attaches ubiquitin to both nuclear regulatory proteins and to misfolded membrane proteins degraded at the ER (ER-associated degradation or ERAD). (yale.edu)
We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)
We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)

Repressor of RNA polymerase III transcription MAF1 homolog is a protein that in humans is encoded by the MAF1 gene. (wikipedia.org)

In the past, most of the attention was focused primarily on protein kinases and on their regulation, mainly because phosphatases were then viewed as simple housekeeping enzymes. (ac.be)
But advances in the understanding of protein phosphatases make now clear that these enzymes are precisely regulated and are as important as kinases in the regulation of cellular processes involving protein phosphorylation. (ac.be)
Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
These auxiliary proteins include RNases, RNA-modification and - remodeling enzymes, transport factors, and many others whose function is unclear at present. (biomedcentral.com)
these proteins include cell cycle proteins, signaling proteins, and protein-processing enzymes. (wikidoc.org)
We discovered the first enzymes that can remove SUMO from other proteins, causing this protein modification to be highly dynamic. (yale.edu)
A third, more recent research focus has been on endosymbiotic bacteria that manipulate their eukaryotic hosts by secreting enzymes, including ubiquitin-specific proteases, into the host cell cytoplasm. (yale.edu)

The RPS23 hydroxylases in S. cerevisiae (Tpa1p), Schizosaccharomyces pombe and green algae catalyze an unprecedented dihydroxylation modification. (ox.ac.uk)

We have analyzed the phenotypic effects of a genomic ubi1∆ub-HA ubi2∆ mutant, which expresses a ubiquitin-free HA-tagged eL40A protein as the sole source of cellular eL40. (123dok.net)
Although intracellular signal transduction is often portrayed as a protein kinase 'domino effect', the counterbalancing function of phosphatases, and thus the control of phosphatase activity, is equally relevant to proper regulation of cellular function. (ac.be)
Although viewed as a constitutive housekeeping enzyme in the past, PP2A is a highly regulated phosphatase and is emerging as an important regulator of multiple cellular processes involving protein phosphorylation. (ac.be)
2 Protein phosphatase 2A (PP2A) is a very abundant - it accounts for as much as 1% of total cellular proteins - ubiquitous and remarkably conserved enzyme. (ac.be)
RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
In particular, as RNA binding proteins, RPs have been found to bind cellular and viral RNAs outside of the context of the ribosome. (prolekarniky.cz)
Downregulation of the corresponding RPN11 gene in combination with high levels of αSyn resulted in depletion of the pool of cellular ubiquitinated proteins and enhanced αSyn mediated toxicity. (uni-goettingen.de)

The yeast genome database [1] is highly annotated and remains a very important tool for developing basic knowledge about the function and organization of eukaryotic cell genetics and physiology. (wikidoc.org)

Eighteen of these were hypothetical proteins with unknown functions, while the others had putative or known functions. (elsevierpure.com)

Here we employ a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which causes global proteome remodeling. (lu.se)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)

Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. (nature.com)
The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large loop domains, although the underlying molecular mechanisms have remained unknown. (nature.com)
The telomeres are the DNA-protein structures at the ends of eukaryotic chromosomes, which serve to protect and stabilize the genome. (lu.se)

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
Ubiquitin and an array of related molecules (ubiquitin-like proteins or Ubls) such as SUMO are small, highly conserved proteins that are covalently attached to other intracellular proteins, resulting in various functional alterations of these targets. (yale.edu)

Recent work constructing protein interaction networks in Saccharomyces cerevisiae suggested the methyltransferase Bud23 was involved in ribosome biogenesis (1). (utexas.edu)

It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology , much like Escherichia coli as the model prokaryote . (wikidoc.org)

Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)

In contrast to most previously characterized accuracy modulators, including antibiotics and the prion state of the S. cerevisiae translation termination factor eRF3, Rps23p hydroxylation can either increase or decrease translational accuracy in a stop codon context-dependent manner. (ox.ac.uk)
Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
In addition, interventions leading to reduced translation, such as dietary restriction and reduced 60S ribosomal protein expression, elicits lifespan extension in yeast, worms and files [10] - [13] . (prolekarniky.cz)
Disadvantages: mainly due to the lack of processing mechanisms after protein translation, such as disulfide bond formation, protein glycosylation and correct folding, the probability of obtaining biologically active proteins is small. (medicilon.com)

Pulsed-SILAC experiments reveals that NatA-deficient strains consistently increase degradation of ribosomal proteins compared to wild type. (lu.se)
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. (nih.gov)
Aspartate/glutamate leucyltransferase (also known as bacterial protein transferase or Bpt) functions in the N-end rule pathway of protein degradation where it conjugates Leu from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate. (embl.de)
We wish to understand at a molecular level how specific eukaryotic proteins are selected for rapid degradation even while most proteins are spared. (yale.edu)

Recent proteomic studies in Saccharomyces cerevisiae have identified nearly 200 proteins, other than the structural ribosomal proteins, that participate in the assembly of ribosomal subunits and their transport from the nucleus. (biomedcentral.com)

The Ubl called SUMO is attached to many proteins in vivo and is crucial for cell-cycle progression. (yale.edu)

We propose that ribosome specificity may exist in mammals, providing evidence that one ribosomal protein can influence composition of the ribosome by regulating its own paralog. (prolekarniky.cz)
We are trying to understand the functional consequences of SUMO-protein modification, particularly in the cell cycle and chromatin-mediate gene transcription, and to determine the basis of specificity for the SUMO-cleaving proteases. (yale.edu)

Protein Phosphatase 2A (PP2A) is a widely expressed family of protein phosphatases made of a core dimer, composed of a catalytic (C) subunit and a structural (A) subunit, in association with a third variable regulatory (B) subunit. (ac.be)

Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
This mutant shows a severe slow-growth phenotype, which could be fully suppressed by increased dosage of the ubi1∆ub-HA allele, or partially by the replacement of ubiquitin by the ubiquitin-like Smt3 protein. (123dok.net)
Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
Normally, the conjugation of ubiquitin to other proteins involves the formation of an isopeptide bond between the α-carboxyl group of the C-terminal glycine of a ubiquitin molecule with an ε-amino group of a specific lysine residue within the target protein [2,3]. (123dok.net)
Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
The prototypical example of such a protein modifier is ubiquitin, but at least a dozen such systems exist. (yale.edu)
While ubiquitin is commonly used to mark its targets for destruction, the consequences of protein ligation to the various "ubiquitin-like proteins" are less understood. (yale.edu)
We also study endosymbiotic bacteria that live in eukaryotic cellsand often manipulate their hosts by causing changes to the ubiquitin system. (yale.edu)
The prototypical example of a protein that is covalently attached to other proteins is ubiquitin, but in recent years, evidence for at least a dozen such systems has come to light. (yale.edu)

Medicilon researchers have established a mature yeast expression and purification service platform to provide high-quality recombinant protein expression and purification services in Pichia and Saccharomyces cerevisiae. (medicilon.com)

Many proteins involved in eukaryotic transcription are similar in function and in sequence between organisms. (embl.de)
The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
A recent further proof of such coordination is the finding that rRNA transcription and rRNA processing are coordinated through a subset of proteins shared by the two processes [ 14 ]. (biomedcentral.com)
Mediating proteins, known as transcription factors, facilitate this process by interacting with the cell 's sDNA and the transcription machinery. (edu.vn)
The first problem is to develop a representation for a group of known binding sites of a particular transcription factor, in order to facilitate recognition of other binding sites of the same protein. (edu.vn)

We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. (cipsm.de)

2004) Mutations causing childhood ataxia with central nervous system hypomyelination reduce eukaryotic initiation factor 2B complex formation and activity. (yeastgenome.org)
Childhood ataxia with central nervous system hypomyelination (CACH), or vanishing white matter leukoencephalopathy (VWM), is a fatal brain disorder caused by mutations in eukaryotic initiation factor 2B (eIF2B). (yeastgenome.org)

However, only a handful of pioneering studies describe the dynamics of proteins in response to external stimuli, despite the fact that regulation of protein levels and localization is an essential part of such responses. (weizmann.ac.il)

Heterologous expression of bacterial virulence factors in Saccharomyces cerevisiae is a feasible approach to study their molecular function. (microbiologyresearch.org)
The proteasome is a molecular machine that fragments proteins into short peptides. (yale.edu)
Understanding molecular interactions is at the core of computational biology and includes problems such as characterizing protein-protein, protein-small molecule, protein-DNA, and Protein-RNA binding events. (edu.vn)
A major objective in molecular biology is to understand how a genome encodes the information that speci es when and where a gene will be transcribed into its protein product. (edu.vn)
Originally cloned from jellyfish and corals, these proteins and their engineered derivatives have become ubiquitous tools in molecular and cell biology. (edu.vn)

Saccharomyces cerevisiae is a single-celled, eukaryotic fungus commonly used in food production. (whatsvegan.app)
At the same time, KMD Bioscience has an expression vector system that can provide customers with higher-purity proteins than the commonly used protein expression vectors on the market. (kmdbioscience.com)

Indeed, vast amounts of data have been collected on transcriptional responses in Saccharomyces cerevisiae. (weizmann.ac.il)
Our findings imply CACH/VWM mutations do not specifically impair responses to eIF2 phosphorylation, but instead cause protein structure defects that impair eIF2B activity. (yeastgenome.org)

Expression of αSyn in yeast results in growth impairment and cytoplasmic protein inclusions resembling the aggregates observed within LBs. A tandem fluorescent protein timer (tFT) was exploited to perform a high-throughput screen for proteins with altered turnover upon expression of αSyn or a phosphorylation-deficient S129A mutant. (uni-goettingen.de)

S. cerevisiae Maf1 is a negative effector of RNA polymerase III (Pol III). (wikipedia.org)

S.M. Paulissen and L.S. Huang (2016) Efficient sporulation of Saccharomyces cerevisiae in a 96 multiwell format. (umb.edu)

S. cerevisiae is currently the only yeast cell known to have Berkeley bodies present, which are involved in particular secretory pathways. (ensembl.org)

Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)

With the increasing demand for commercially important proteins, it has become essential to develop more improved and efficient methods for heterologous protein production to meet the requirements of society. (benthamscience.com)
Different host systems that are used for heterologous protein expression have their own advantages and disadvantages as in the case of bacterial systems, a good amount of protein is produced but the quality of the protein may not be appropriate. (benthamscience.com)
In addition to this, we will also elaborate on different aspects related to the overexpression and purification of recombinant heterologous protein and the type of tags and chromatographic techniques used for the purification of different types of proteins. (benthamscience.com)
Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
Fluorescent proteins are intrinsically fluorescent, genetically encodable tags that can be expressed in many heterologous organisms. (edu.vn)

However, it is likely that several horizontal gene transfers (HGT) also occurred in eukaryotic microorganisms and, even more surprisingly, in bacteria. (biomedcentral.com)

This study corroborates a complex mechanistic interplay between proteasome and pS129 αSyn causing a substantial altered protein homeostasis in yeast as model for PD. (uni-goettingen.de)

SNARE proteins are subject to multiple levels of regulation, one of which is imbedded in the syntaxin (Qa-SNARE) molecule. (gla.ac.uk)
Regulation of peroxisomal matrix protein import by ubiquitination. (ox.ac.uk)
Dr. Amitabha Mukhopadhyay, National Institute of Immunology, New Delhi- Regulation of Rab proteins by cytokines. (jnu.ac.in)

C.J. Slubowski, S.M. Paulissen, and L.S. Huang (2014) The GCKIII kinase Sps1 and the 14-3-3 isoforms, Bmh1 and Bmh2, cooperate to ensure proper sporulation in Saccharomyces cerevisiae. (umb.edu)

However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. (lu.se)
Although currently there are different host systems available for the production of proteins heterologously, still the selection of a perfect host system becomes a tedious task if the aim is to get fully functional protein in adequate quantity. (benthamscience.com)
The selection of the appropriate host depends on the amount of the protein, quality of the protein in terms of functional activity and its application. (benthamscience.com)
In addition to investigating the functional significance of Vps45p's interaction(s) with Tlg2p, I have also investigated binding of the SM protein to the v-SNARE Snc2p. (gla.ac.uk)
Finally, I have developed an in vitro fusion assay to enable us to dissect the functional significance of the various interactions that Vps45p displays with its cognate SNARE proteins. (gla.ac.uk)
This method has applications in protein purification , localization, and functional analysis. (medicilon.com)

b ) Volcano plot showing the distribution of proteins according to their enrichment in crosslinked (CL) over non-CL samples. (nature.com)

Current work in the lab focuses on how these morphogenic events are regulated by specific proteins in the cell. (umb.edu)

Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)

ellipsoideus389391, com.sigma.fs3.argos.domain.gpc.GpcArticlesRev[id=com.sigma.fs3.argos.domain.gpc.GpcArticlesRevPK[ifcactivitat=ARE, ifccomptador=6940]], camposKey:0302-8933 1991-01-01 Effect of l-azetidine 2-carboxilic acid on the activity of the general amino-acid permease from Saccharomyces cerevisiae var. (uva.es)

Chitin has also been sporadically found in structures from a diverse range of eukaryotic microorganisms, such as the cell wall of a few chlorophyta (green microalgae), the cyst wall or lorica, of ciliophora (ciliated protozoans), the theca of choanoflagellida (flagellated protozoans), and the test or cyst wall, of amoebozoa (amoeboid protozoans) [ 6 ]. (biomedcentral.com)
Hopfner, K. P. & Tainer, J. A. Rad50/SMC proteins and ABC transporters: unifying concepts from high-resolution structures. (nature.com)

Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the multiple modes of interaction that have been characterised for different members of this family binding to their cognate SNARE proteins. (gla.ac.uk)

Anatomy22

Organisms14

Diseases1

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes90

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Yeast Saccharomyces cerevisiae11

Highly conserved in eukaryotic cells2

Organism6

Mammalian8

Recombinant proteins2

Ribosomes8

Cells21

Elongation4

Post-translational4

Homologous4

Synthesis11

Species6

Prokaryotic6

Genes5

Eukaryotes4

Fermentation1

Membrane9

Humans1

Enzymes8

Schizosaccharomyces1

Cellular7

Genetics1

Hypothetical proteins1

Proteomics2

Chromosomes3

Intracellular2

Ribosome biogenesis1

Intensively studied1

Kinases1

Translation4

Degradation4

Subunits1

Vivo1

Specificity2

Subunit1

Ubiquitin9

Pichia1

Transcription5

Complexes1

Initiation factor2

Stimuli1

Molecular5

Commonly2

Responses2

Turnover1

Polymerase1

20161

Secretory1

Homologue1

Heterologous5

Bacteria1

Proteasome1

Regulation3

Isoforms1

Functional6

Distribution of proteins1

Specific proteins1

Expression System1

Amino1

Structures2

Interaction1