Estrogen nuclear mcf 7 cells. Medical search. Frequent questions

Estrogen receptors (ERs) are a group of proteins found inside cells. (wikipedia.org)
They are receptors that are activated by the hormone estrogen (17β-estradiol). (wikipedia.org)
Two classes of ER exist: nuclear estrogen receptors (ERα and ERβ), which are members of the nuclear receptor family of intracellular receptors, and membrane estrogen receptors (mERs) (GPER (GPR30), ER-X, and Gq-mER), which are mostly G protein-coupled receptors. (wikipedia.org)
Hormone-activated estrogen receptors form dimers, and, since the two forms are coexpressed in many cell types, the receptors may form ERα (αα) or ERβ (ββ) homodimers or ERαβ (αβ) heterodimers. (wikipedia.org)
Different ligands may differ in their affinity for alpha and beta isoforms of the estrogen receptor: estradiol binds equally well to both receptors estrone, and raloxifene bind preferentially to the alpha receptor estriol, and genistein to the beta receptor Subtype selective estrogen receptor modulators preferentially bind to either the α- or the β-subtype of the receptor. (wikipedia.org)
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. (cancerindex.org)
This in vitro study should analyze the changes in expression of cofactors of the estrogen receptors in human breast cancer cells exposed to low frequency electromagnetic fields . (emf-portal.org)
Tamoxifen is applied in estrogen receptor positive breast cancer therapy as it binds to estrogen receptors of breast cancer cells and through this prevents their proliferation . (emf-portal.org)
As estrogen receptors can be modified by cofactors , gene expression of these cofactors (coactivator: AIB1, SRC-1, corepressor: N- Cor , SMRT) should be elucidated. (emf-portal.org)
Both E2 and E1 bind to estrogen receptors (ERs), which have been highly studied in the breast, uterus, and ovary. (eneuro.org)
These cells process estrogen, in the form of estradiol, via cytoplasmic estrogen receptors. (mybio.ie)
Thus, there is a huge demand for estrogen replacement therapeutics acting on beta estradiol receptors but not activating alpha estrogen receptors and thus increasing the risk to develop cancer. (vivacell.de)
Structure-activity relationships of FXR-active compounds revealed by this screening were then compared against the androgen receptor, estrogen receptor α, peroxisome proliferator-activated receptors δ and γ and the vitamin D receptor. (nature.com)
However, nuclear receptors are also associated with melatonin activity. (explorationpub.com)
1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
Estrogen receptor related ON 146040 receptors (ERRs), which include ERR//, are orphan nuclear receptors and share sequence homology with estrogen receptor (ER) 15. (biotech2012.org)

The prolyl oligopeptidase inhibitor SUAM-14746 attenuates the proliferation of human breast cancer cell lines in vitro. (cancerindex.org)
The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
It is reported that in tumors which express higher amounts of coactivators and less corepressors tamoxifen induces proliferation of the breast cancer cells and significantly reduces the disease -free survival. (emf-portal.org)
The two isoforms of estrogen receptor (ER) alpha and beta play opposite roles in regulating proliferation and differentiation of breast cancers, with ER-alpha mediating mitogenic effects and ER-beta acting as a tumor suppressor. (vivacell.de)
In breast cancer, LRH-1 stimulates proliferation and increases intratumoural estrogen levels. (asnevents.com.au)
Here, we aimed to determine the effects of hnRNPs and LRH-1 in regulating breast cancer cell proliferation and metabolism. (asnevents.com.au)
Cell proliferation and lactate production measured to assess the effect of LRH-1 and hnRNPs on cell metabolism. (asnevents.com.au)
0.01) decreased proliferation rate in LRH-1 knockdown and hnRNPA1 knockdown cells. (asnevents.com.au)
Our results indicate that knocking down LRH-1 reduced hnRNPA1 and hnRNPA2/B1 expression, decreasing aerobic glycolysis as evidenced by a reduction in lactate production and decreased cell proliferation. (asnevents.com.au)
Luminal-like breast tumor cells express estrogen receptor alpha (ER-alpha) , a member of the nuclear receptor family of ligand-activated transcription factors that controls their proliferation, survival, and functional status. (unica.it)
Together, our results suggest that ANKRD45 is a novel ankyrin repeat protein with a conserved role during cell proliferation in both zebrafish embryos and mammalian cells. (innovation-ecosystems-agora.com)
The GTP-bound KRASG12V proteins chronically bind to and activate multiple downstream signaling pathways, including MAPK or PI3K/AKT signals, which lead to excessive cell proliferation and subsequent carcinogenesis [20]. (innovation-ecosystems-agora.com)
We have recently demonstrated that a natural dietary supplement BreastDefend (BD), which contains extracts from medicinal mushrooms (Coriolus versicolor, Ganoderma lucidum, Phellinus linteus), medicinal herbs (Scutellaria barbata, Astragalus membranaceus, Curcuma longa), and purified biologically active nutritional compounds (diindolylmethane and quercetin), inhibits proliferation and metastatic behavior of MDA-MB-231 invasive human breast cancer cells in vitro. (spandidos-publications.com)
Quercetin, a flavonoid found in fruits, vegetables, leaves and grains inhibited proliferation of invasive breast cancer cells and its combination with other polyphenols further suppressed tumor growth and site-specific metastasis ( 15 , 16 ). (spandidos-publications.com)
Many laboratories have focused on the functions of the clock genes and proteins that are involved in cellular activities, including cell survival, cell cycle regulation, proliferation, apoptosis, DNA damage repair, and tumor promotion or suppression [ 5 , 6 ]. (explorationpub.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)

In addition, estradiol treatment of MCF-7 cells causes inhibition of apoptosis. (mybio.ie)
In contrast, ER beta has a well known growth and invasion inhibitory activity in ERα-positive breast cancer cells, at least in part due to ER beta's inhibition of ER alpha selective target gene expression, and can be considered as an endogenous partial dominant negative receptor. (vivacell.de)
Although SLTM also suppressed the ability of oestrogen to activate a reporter gene in MCF-7 breast-cancer cells, inhibition of a constitutively active beta-galactosidase gene suggested that this was primarily the consequence of a generalized inhibitory effect on transcription. (bris.ac.uk)
IB may also induce apoptosis in cancer cells as inhibition of IKKa, an IB kinase leading to its degradation, can switch the effects of estrogens on human breast cancer MCF-7 cells from anti- to pro-apoptotic [12,13]. (innovation-ecosystems-agora.com)
AntagomiRNA-mediated inhibition of miRNA-9 increased both PP2A activity and GR nuclear translocation in macrophages, and restored steroid sensitivity in isolated macrophages and in several mouse models of steroid-resistant AHR [ 2 ] . (encyclopedia.pub)
As determined using XTT cell growth and survival assays, MIH 2.4Bl exhibited growth inhibition activity on most breast cancer cell lines tested, compared with normal human mammary epithelial cells. (lsu.edu)
Three breast cancer cell lines (MCF-7, T-47D, and ZR-75-1) showed a more potent sensitivity index to growth inhibition by MIH 2.4Bl than the other breast cancer cell lines. (lsu.edu)
Melatonin has oncostatic properties that are mediated by induction of apoptosis, arresting the cell cycle, regulation of metabolic activity, and the inhibition of hypoxia-inducible factor 1α (HIF-1α) activity [ 9 ]. (explorationpub.com)

The anti-cancer activity of ATG has been reported to due to the induction of apoptosis mediated by mitochondrial disruption and cell cycle arrest in breast, lung, bladder, gastric, hepatic, and colon cancer cells [14,15,16,17,18]. (researchassistantresume.com)
In addition, analysis of cell-cycle parameters, chromatin condensation and cytochrome c release showed that SLTM induced apoptosis in a range of cultured cell lines. (bris.ac.uk)
Further, histological analysis suggested that multiple cysts developed in the mutant liver due to cell apoptosis. (innovation-ecosystems-agora.com)
Similarly, knockdown of expression with either siRNA or CRISPR/Cas9 methods induced apoptosis in cultured cells, similar to those in zebrafish mutant livers after induction. (innovation-ecosystems-agora.com)
The ability of E2F-1 to prompt apoptosis in DNA damaged cells coincides with enhanced PRMT1 methylation. (embl.de)
The cytotoxic effect of MIH 2.4Bl on breast cancer cell lines was determined by XTT and crystal violet assays, flow cytometry analysis, electron microscopy characterization, and terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) apoptosis assays. (lsu.edu)
Results of a TUNEL assay performed on cells treated for 96 hours with MIH 2.4Bl supported the observation of apoptosis. (lsu.edu)
Significant gene list was further analysed by Gene Set Enrichment Analysis (GSEA), and the Normalized Enrichment Score (NES) showed that biological processes such as inflammation, protein transport, apoptosis, and cell redox homeostasis were modulated in senescent fibroblasts treated with γ -tocotrienol. (afpm.org.my)
For example, extracts or purified compounds from Scutellaria barbata, Astragalus membranaceus and Curcuma longa suppressed growth, induced oxidative stress and apoptosis, inhibited breast cancer cell invasiveness and prevented breast cancer metastases in mice ( 11 - 14 ). (spandidos-publications.com)
Further studies showed that apoptosis and replication may work together to enhance the cytotoxicity of oncoVV-lectins in a cell-type dependent manner. (mdpi.com)
This indolamine advances apoptosis, which arrests the cell cycle and regulates metabolic activity. (explorationpub.com)

Like mammary epithelial cells, MCF-7 cells are sensitive to estrogen. (mybio.ie)
It is also expressed on endothelial cells, epithelial cells, bone marrow stroma cells, and osteoclasts, as well as a small proportion of LGL lymphocytes. (healthweblognews.info)

In a recent study, we showed ATG suppressed metastatic potential and induced autophagic cell death by inhibiting estrogen receptor (ER) expression in MCF-7 human breast cancer cells [19,20]. (researchassistantresume.com)

The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. (mybio.ie)
Finally, 3,3′-diindolylmethane (DIM), the biologically active compound derived from the digestion of indole-3-carbinol, found in cruciferous vegetables such as broccoli, Brussels sprouts, cabbage and kale suppressed growth, migration and invasion of metastatic breast cancer cells ( 17 , 18 ). (spandidos-publications.com)

To identify the molecular determinants of this hormone-re- sponsive tumor phenotype , a comprehensive genome- wide analysis was performed in estrogen stimulated MCF-7 and ZR-75.1 cells by integrating time-course mRNA expression profiling with global mapping of genomic ER-alpha binding sites by chromatin immunopre- cipitation coupled to massively parallel sequencing , mi- croRNA expression profiling, and in silico analysis of transcription units and receptor binding regions iden- tified. (unica.it)
The upregulated ERR in chemoresistant malignancy cells might be due to improved levels of N6-methyladenosine (m6A) can result in the splicing of precursor mRNA. (biotech2012.org)
A potential function of ERR indicators in chemoresistance was after that evaluated by quantifying the mRNA degrees of ERR (chemoresistance of cancers cells, we set up mouse xenograft tumors through the use of sh-ERR-transfected HepG2/ADR cells (Amount S2G). (biotech2012.org)
qRT-PCR demonstrated ON 146040 that si-ERR considerably decreased the appearance of mRNA amounts in MCF-7/Taxes and MDA-MB-231/Taxes cells (Amount S3A). (biotech2012.org)
Expression and estrogen sensitivity of target genes and nuclear receptor coregulators were altered at mRNA and protein levels in adult uterus, prostate and brain. (researchgate.net)

Mutation of p16, a tumor suppressor protein with four ankyrin repeats, is associated with several human cancers due to abnormal cell cycle defects [6,7]. (innovation-ecosystems-agora.com)
Taken together with the fact that 60-70% of human breast cancers are estrogen receptor-alpha positive[ 7 ], the evidence suggests an etiological significance of estrogen in breast cancer initiation and progression. (biomedcentral.com)
1995. Organochlorine compounds and estrogen-related cancers in women. (cdc.gov)
Each one of these data claim that ERRs tend involved with chemoresistance of cancers cells. (biotech2012.org)
We discovered that ERR was upregulated in chemoresistant cancers cells considerably, with knockdown of ERR rebuilding the chemosensitivity. (biotech2012.org)
Mechanistically, ERR can mediate the chemoresistance of cancers cells via upregulation of and facilitation of FAO. (biotech2012.org)
Outcomes ERR is normally upregulated in chemoresistant cancers cells The chemoresistance of breasts (MCF-7/ADR) and liver organ (HepG2/ADR) cancers cells was verified by evaluation of Dox awareness and weighed against that of their matching parental cells (Amount S1A and S1B). (biotech2012.org)
These data recommended that ERR regulates both and chemoresistance of cancers cells. (biotech2012.org)
P-gp is normally involved with ERR-regulated chemoresistance of cancers cells ABC transporters are crucial for chemoresistance of cancers cells 6. (biotech2012.org)
Appearance from the main ABC transporters, was and including assessed in chemoresistant cancers cells transfected with si-ERR. (biotech2012.org)
Triple-negative breast cancer (TNBC), which lacks the expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), accounts for 10-15% of breast cancers [ 2 ]. (nature.com)

Active Motif's MCF-7 nuclear extract (Estradiol treated) was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. (mybio.ie)
Treatment of with estradiol induces a proliferative response by activating the expression of early and delayed hormone-responsive genes required for cell cycle progression. (mybio.ie)
All 1270 genes that were found to respond to 17beta-estradiol in both cell lines cluster in 33 highly con- cordant groups , each of which showed defined kinetics of RNA changes. (unica.it)
MicroRNAs are also integral components of this gene regulation net- work because miR-107, miR-424, miR-570, miR-618, and miR-760 are regulated by 17beta-estradiol along with other microRNAs that can target a significant number of transcripts belonging to one or more estrogen-respon- sive gene clusters. (unica.it)
There are a total of nine estrogens in humans of which 17β-Estradiol (E 2 ) is the most abundant in circulation and the most biologically active[ 8 ]. (biomedcentral.com)

In vivo FES uptake was measured in tumor xenografts by using small-animal positron emission tomographic/computed tomographic imaging of 24 mice (17 WT ER tumors, nine mutant G521R ER tumors, eight MDA-MB-231 tumors, and four MCF-7 ER-positive tumors). (wisc.edu)
Interestingly, these 3 cell lines were derived from tumors of Luminal A origin and have ER (estrogen receptor), PR (progesterone receptor), and HER2 (human epidermal growth factor receptor 2) positive expression. (lsu.edu)
A safe differentiation of infection from malignant tumors with atypical infiltrative growth pattern such as transitional cell carcinomas hence does not seem feasible, based on the ADC values only.CT of infectious renal disease is still the mainstay of imaging in radiology. (phosphorylaseinhibitors.com)
111 In-R1507 SPECT and 89 Zr-R1507 PET images of mice with subcutaneous SUM149 tumors were acquired at 1, 3, and 7 d after injection. (snmjournals.org)
Triple-negative breast tumors are estrogen receptor-, progesterone receptor-, and HER2 / neu -negative. (snmjournals.org)
Although they account for only 10%-17% of all breast carcinomas ( 1 - 8 ), tumors are often high grade and patients are relatively young and have a reduced breast cancer-specific survival, compared with patients with estrogen receptor- or HER2 / neu -expressing tumors ( 2 , 9 ). (snmjournals.org)
They have complementary mechanisms to hinder the carcinogenic process by searching free radicals, inhibiting survival and multiplication of malignant cells, as well as reducing invasiveness and angiogenesis of tumors. (researchsquare.com)
TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)

The present study tested the hypothesis that the SFP can regulate CYPs in vitro in HepG2 cells and in vivo in Walker-256 tumor-bearing rats. (bvsalud.org)
The in vitro digestion and nuclear magnetic resonance analyses suggested that SFP was minimally modified in the gastrointestinal system. (bvsalud.org)
Here, we examined the role of CRV in breast cancer cell adhesion, migration and invasion in vitro and how this component could suppress the growth of Ehrlich carcinoma-bearing mice. (bvsalud.org)
Further mechanistic investigation in vitro revealed that CRV modulates the interaction of breast cancer cells with the extracellular matrix (ECM) by disrupting focal adhesion, which was shown by scanning electron microscopy (SEM) and immunofluorescence. (bvsalud.org)
MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. (mybio.ie)
FES binding was measured by using an in vitro cell uptake assay. (wisc.edu)
In vitro FES binding in the 231 G521R ER cells was reduced to that observed in the parental cells. (wisc.edu)
In our work, we evaluated the in vitro anticancer effects of HS extract against two different human breast cancer cell lines: estrogen receptor (ER) positive MCF-7 cells and ER negative MDA-MB-231 cells. (fupress.net)
Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)
BreastDefend™ (BD) is a polybotanical dietary supplement which inhibits growth and invasive behavior of highly metastatic human breast cancer cells in vitro ( 6 ). (spandidos-publications.com)
In vitro, the affinity and internalization kinetics of 111 In-R1507 were determined using the IGF-1R-expressing triple-negative breast cancer cell line SUM149. (snmjournals.org)

Once activated by estrogen, the ER is able to translocate into the nucleus and bind to DNA to regulate the activity of different genes (i.e. it is a DNA-binding transcription factor). (wikipedia.org)
In humans, the two forms of the estrogen receptor are encoded by different genes, ESR1 and ESR2 on the sixth and fourteenth chromosome (6q25.1 and 14q23.2), respectively. (wikipedia.org)
Our previous expression profiling identified the heterogeneous ribonucleoproteins (hnRNPs) as potential LRH-1 target genes in MCF-7 cells. (asnevents.com.au)
Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
Let-7f miRNAs belong to a highly conserved let-7 (lethal-7) microRNA family consisting of 12 genes encoding for nine different miRNAs (let-7a to let-7i). (encyclopedia.pub)
Once formed, the complex translocates to the nucleus where it acts as a transcription factor by binding to the estrogen response elements (EREs) at the promoters of estrogen responsive genes[ 9 , 10 ]. (biomedcentral.com)
Scaffoldattachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind toS/MAR. The encoded protein is thought to serve as a molecular base to assemble atranscriptosome complex in the vicinity of actively transcribed genes. (immune-source.com)
Genes to Cells. (elsevierpure.com)

It is involved in theregulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressorand is a candidate for breast tumorigenesis. (immune-source.com)

Together, these results show that estrogens regulate nicotine neurobiology, but additional factors may be required to restore nicotine consumption to ovary-intact levels. (eneuro.org)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)

Using different cell lines, we show that the distribution of ANKRD45 protein was highly dynamic during mitosis. (innovation-ecosystems-agora.com)
cell signaling Notch protein, and later named after the human membrane-associated ankyrin protein, which contains 24 such repeats and regulates the interaction between the cytoskeleton and the plasma membrane [3,4]. (innovation-ecosystems-agora.com)
The Ro 10-5824 dihydrochloride ANK protein IB, an inhibitor of nuclear factor kappa B (NF-B), is involved in transcription regulation and mediates metabolism and inflammatory responses [10,11]. (innovation-ecosystems-agora.com)
Furthermore, because MGMT protein loss may be a result of promoter hypermethylation, it was reported that methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness to alkylating agents ( 7 ). (aacrjournals.org)
ERα transcriptional function was abolished in the mutated 231 G521R ER cells despite appropriate receptor protein expression and nuclear localization. (wisc.edu)
Protein kinase C regulates human pluripotent stem cell self-renewal. (rndsystems.com)
MiRNA-9 production decreased protein phosphatase 2A (PP2A) activity and also inhibited steroid-induced glucocorticoid receptor (GR) nuclear translocation. (encyclopedia.pub)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)
This protein is thought to be involved inattaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as towhether this protein is a component of chromatin or a nuclear matrix protein. (immune-source.com)

These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. (mybio.ie)

In the present study, we evaluated whether BD suppresses growth and breast-to lung cancer metastasis in an orthotopic model of human breast cancer cells implanted in mice. (spandidos-publications.com)
One of the major reasons for such a high morbidity and mortality of breast cancer is the invasive behavior of breast cancer cells leading to cancer metastasis. (spandidos-publications.com)
In the present study, we evaluated toxicity and anti-cancer activities of BD in an animal model of breast-to-lung cancer metastasis with triple-negative highly invasive humane breast cancer cells MDA-MB-231 implanted in the mammary pad of nude mice. (spandidos-publications.com)

A,B) Cell viabilities were determined using an MTT assay. (researchassistantresume.com)
MTT assay and Trypan Blue vital count showed a dose and time dependent reduction of the viability in both cell lines treated with different concentrations of HSE or HSEC compared to untreated control cells. (fupress.net)
Using a scratch wound healing assay we did not observe any change in the migration of cells compared to untreated cells. (fupress.net)
On the contrary, in a Boyden chamber invasion assay, HSE, and especially HSEC, induced reduction of MCF-7 cell invasion. (fupress.net)
We used a cell-based human FXR β-lactamase ( Bla ) reporter gene assay to profile the Tox21 10K compound collection of environmental chemicals and drugs. (nature.com)

Both ERs are widely expressed in different tissue types, however there are some notable differences in their expression patterns: The ERα is found in endometrium, breast cancer cells, ovarian stromal cells, and the hypothalamus. (wikipedia.org)
To investigate the correlation between the polymorphism of estrogen receptor β gene (ESR2) rs3020450 and cancer susceptibility, and explore the epidemiological significance and the effect of ESR2 expression levels on the prognosis of ovarian cancer. (cancerindex.org)
Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells med. (emf-portal.org)
The gene expression of both coactivators were more strongly expressed in the exposed breast cancer cells while the gene expression of the two corepressors decreased. (emf-portal.org)
The observed increased estrogen receptor coactivators expression and decreased estrogen receptor corepressors can explain the decrease in efficacy of tamoxifen during exposure to electromagnetic fields . (emf-portal.org)
The SFP reduced total CYP levels in the solid and liquid tumor protocols and reduced the gene expression of Cyp1a1 and Cyp2e1 in rats and CYP1A2 in HepG2 cells. (bvsalud.org)
Transcriptional Regulation of the Angptl8 Gene by Hepatocyte Nuclear Issue-1 within the Murine Liver Temporary refeeding instances (~60 min) enhanced hepatic Angptl8 expression in fasted mice. (nfkb-p65.org)
that ATG enhances the cytotoxic activity of DOX in MDA-MB-231 human breast cancer cells by inducing prolonged p21 expression and p38-mediated AIF-dependent cell HOXA11 death. (researchassistantresume.com)
LRH-1 expression was knocked down by siRNA in two breast cancer cell lines, MDA-231 and MCF-7. (asnevents.com.au)
In addition, expression of pyruvate kinase M2 (PKM2) was reduced and PKM1 levels increased in LRH-1 and hnRNPA1 knockdown cells. (asnevents.com.au)
The objective of this study was to comprehensively characterize the effects of estrogen and progesterone on the expression and activity of major drug-metabolizing P450s. (aspetjournals.org)
Taken together, our results show differential effects of estrogen and progesterone on P450 expression, suggesting involvement of different regulatory mechanisms in female hormone-mediated P450 regulation. (aspetjournals.org)
Untreated MDA-MB-231 cells showed a low expression of the receptor mostly localized at the cytoplasmic level and treatment with HSE or HSEC didn't change this state. (fupress.net)
Untreated MCF-7 cells showed a greater expression of the receptor, with nuclear and cytoplasmic localization. (fupress.net)
Among the tumor suppressor miRNAs, reduced let-7 expression occurs most frequently in cancer and typically correlates with poor prognosis. (encyclopedia.pub)
When individual members of the let-7 family were measured, the expression of let-7f was reported to be higher in cultured epithelial brushings from asthmatic donors [ 1 ] . (encyclopedia.pub)
Let-7f expression was lower and IL-23R expression was higher in TH17-differentiated cells from women compared with men with severe asthma [ 16 ] . (encyclopedia.pub)

In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)

To test the effect of estrogen on nicotine use, we ovariectomized (OVX) female rats that then underwent nicotine self-administration acquisition and compared them to ovary-intact (sham) rats. (eneuro.org)
The effect of estrogen on the preservation of bone is well documented, and bone loss begins with a decrease in estrogen levels during the perimenopausal period. (vivacell.de)

Some isoforms dominantly inhibit the activity of other estrogen receptor family members. (cancerindex.org)

Role of RIP140 in estrogen and E2F pathways. (scirp.org)
Melatonin can disrupt estrogen-mediated cellular pathways, ensuing in a lattice drop of estrogenic stimulus to the breast cancer cells. (explorationpub.com)
Sensitization of cancer cells to conventional drugs using multi-target agents that block survival and oncogenic pathways, alone or in combination, is an emerging strategy to overcome drug resistance. (researchsquare.com)
We created cisplatin-resistant MCF-7 cells and determined the practical standing of epidermal growth aspect receptor (EGFR), MAPK, and AKT signaling pathways by phosphoreceptor tyrosine kinase and phospho-MAPK arrays. (angiotensinreceptor.com)
The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)

In conclusion, we have demonstrated that HS is able to reduce cell viability of ER positive MCF-7 and ER negative MDA-MB-231 cells. (fupress.net)

citation needed] The N-terminal A/B domain is able to transactivate gene transcription in the absence of bound ligand (e.g., the estrogen hormone). (wikipedia.org)

Our data provide evidence that estrogen signaling mediates the tumor vasculogenic process required for breast cancer progression and involves a key regulator of the hypoxia signaling pathway. (biomedcentral.com)

Subsequently, we studied HSE and HSEC ability to alter migration and invasion capacity of ER positive MCF-7 cells. (fupress.net)

For in vivo studies, solid Ehrlich carcinoma (SEC) was used, a syngeneic mouse model with non-nuclear estrogen and progesterone positivity, characterized by immunohistochemistry. (bvsalud.org)
We identified 36 new putative partners for PRMT6 and we validated the interaction in vivo for 7 of them. (embl.de)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)

Regional hepatic CYP1A1 and CYP1A2 induction with 2,3,7,8-tetrachlorodibenzo- p -dioxin evaluated with a multicompartment geometric model of hepatic zonation. (cdc.gov)

The C domain, also known as the DNA-binding domain, binds to estrogen response elements in DNA. (wikipedia.org)

Collectively, these studies demonstrate the important contributions of estrogens to nicotine neurobiology and highlight differences in how ovarian hormones impact nicotine neurobiology and behavior. (eneuro.org)
understanding the role of ovarian hormones and specifically, estrogens in maintaining nicotine use are essential. (eneuro.org)
Development of a recombinant human ovarian (BG1) cell line containing estrogen receptor alpha and beta for improved detection of estrogenic/antiestrogenic chemicals. (cellosaurus.org)

From a methanol/methylene chloride extract of C. inophyllum, the same natural products in considerably greater yield were isolated in addition to a novel enantiomer of soulattrolide (4), inophyllum P (2b), and two other novel compounds, inophyllums G-1 (7) and G-2 (8). (afpm.org.my)

However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)

To gain insight to the mechanisms underlying cisplatin resistance in breast cancer, we used estrogen receptor-positive MCF-7 cells as being a model method. (angiotensinreceptor.com)

In previous studies (see publication 1254 , publication 5621 ) a decrease in efficacy of the selective estrogen receptor modulator tamoxifen was observed in breast cancer cells exposed to electromagnetic fields . (emf-portal.org)
The cytotoxic potential of 4NC was tested towards MCF-7 and MDA-MD-231 breast cancer cells, with a lower impact in the non-tumor lineage HB4a. (bvsalud.org)
reported human non-small cell lung cancer (NSCLC) cells treated with ATG exhibited greater chemosensitivity to cisplatin-induced apoptotic cell death mediated by the down-regulation of survivin [21]. (researchassistantresume.com)
Numerous investigations have shown ATG has anti-cancer and anti-metastatic effects on different cancer cell types. (researchassistantresume.com)
Synergistic cytotoxic activity of ATG/DOX co-treatment was observed in MDA-MB-231 human triple negative breast cancer cells. (researchassistantresume.com)
These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer. (mybio.ie)
Unopposed estrogen therapy in standard doses is associated with an increased risk of endometrial hyperplasia and cancer, which can be attenuated by concurrent administration of a progestin. (vivacell.de)
ER alpha mediates cancer-promoting effects of estrogen and has been shown to be an effective therapeutic target for decades. (vivacell.de)
New Therapeutic Procedure To Eliminate Cancer Cells Infiltrated In Brain. (usherbrooke.ca)
To determine the binding specificity of 18F-16α-17β-fluoroestradiol (FES) in estrogen receptor (ER) α-positive breast cancer cells and tumor xenografts. (wisc.edu)
Let-7f was negatively regulated by estrogen receptor signaling in the MCF-7 breast cancer cell line [ 15 ] . (encyclopedia.pub)
Epidemiological studies show a rapid increase in breast cancer incidence during reproductive years that tapers around age 50, corresponding to the onset of menopause, and studies of postmenopausal breast cancer patients have found a higher level of estrogen in breast tissue compared to healthy patient tissue[ 1 - 6 ]. (biomedcentral.com)
In addition to its normal physiological roles, estrogen is also implicated in breast cancer initiation and progression. (biomedcentral.com)
Interestingly, estrogen is also capable of contributing to breast cancer progression by a novel role, via modulation of proteins involved in hypoxia signaling, namely hypoxia inducible factor 1 (HIF-1). (biomedcentral.com)
In this work, we report the synthesis of a new 1,3-thiazolium-5-thiolate derivative of a mesoionic compound (MIH 2.4Bl) and the characterization of its selective cytotoxicity on a panel of breast cancer cells lines. (lsu.edu)
BD contains mycelium from Asian medicinal mushrooms ( Coriolus versicolor, Ganoderma lucidum, and Phellinus linteus ), which separately suppressed growth and inhibited invasiveness of breast cancer cells by different mechanisms ( 6 - 10 ). (spandidos-publications.com)
In addition, some of the natural agents in BD also demonstrated anticancer activities against breast cancer cells. (spandidos-publications.com)
Our previous studies demonstrated that arming vaccinia viruses with marine lectins enhanced the antitumor efficacy in several cancer cells. (mdpi.com)
This study aims to compare the efficacy of oncolytic vaccinia viruses harboring Tachypleus tridentatus lectin (oncoVV-TTL), Aphrocallistes vastus lectin (oncoVV-AVL), white-spotted charr lectin (oncoVV-WCL), and Asterina pectinifera lectin (oncoVV-APL) in breast cancer cells (BC). (mdpi.com)
Melatonin has oncostatic properties in breast cancer cells. (explorationpub.com)
With the synchronized output of the central circadian clock in relation to the pineal gland and other peripheral oscillators, melatonin acts as a key role player in the regulation of intermediary metabolism and cancer prevention [ 7 ]. (explorationpub.com)
Excessive exposure to estrogen is a key risk factor for breast cancer [ 10 ]. (explorationpub.com)
Cancer is characterized as the uncontrolled multiplication and spread of the body's particular cells. (researchsquare.com)
Cancer Cell International. (elsevierpure.com)

TMB (A) Cells were incubated in Dulbeccos Modified Eagles medium (DMEM) medium containing various concentrations TMB of DOX (0C1 M) for 24, 48, or 72 h. *, ** and # indicate (researchassistantresume.com)
Ultrastructural analysis of MFC-7 cells treated with MIH 2.4Bl at 2 different concentrations (37.5 and 75 μM) was performed by transmission electron microscopy. (lsu.edu)
Nonetheless, current evidence seems conflicting regarding the effects of cannabis on male reproductive endocrine function, with an emphasis on the inconclusive findings in testosterone levels, concomitant with reduced luteinizing hormone (LH) and unchanged follicle-stimulating hormone (FSH) concentrations ( 7 - 12 ). (frontiersin.org)

Background Loss of function of fumarate hydratase (FH), the mitochondrial tumor suppressor and tricarboxylic acid (TCA) cycle enzyme, is associated with a highly malignant form of papillary and collecting duct renal cell malignancy. (immune-source.com)

Characteristics: Acts through the activation of a luciferase reporter gene under the control of estrogen response elements (EREs) upstream of the mouse mammary tumor viral (MMTV) promoter. (cellosaurus.org)

In contrast, cyclin A binding to E2F-1 impedes PRMT1 methylation and augments PRMT5 methylation, thus ensuring that E2F-1 is locked into its cell-cycle progression mode. (embl.de)

Conclusions: m6A induced ERR confers chemoresistance of malignancy cells through upregulation of and CPT1Bgrowth of malignancy cells 13, 14. (biotech2012.org)
However, the functions and regulatory factors of FAO in chemoresistant malignancy cells are mainly unfamiliar. (biotech2012.org)

MCF-7 cells displayed both time- and dose-dependent decreases in cell growth and survival, with a maximum cytotoxic effect observed at 72 and 96 hours. (lsu.edu)

In addition, the different estrogen receptor combinations may respond differently to various ligands, which may translate into tissue selective agonistic and antagonistic effects. (wikipedia.org)
The concept of selective estrogen receptor modulators is based on the ability to promote ER interactions with different proteins such as transcriptional coactivator or corepressors. (wikipedia.org)

Estrogen mediates its effects by binding to its cognate estrogen receptor(s), either estrogen receptor alpha (ERα) or estrogen receptor beta (ERβ), leading to ER dimerization and association with various co-factors. (biomedcentral.com)

Therefore, we assessed the effects of ATG/DOX co-treatment to determine whether ATG enhances the cytotoxic effect of DOX in MDA-MB-231 TNBC cells. (researchassistantresume.com)

This effect is more evident in MCF-7 cells in which ER localization and reduced cell invasion were observed. (fupress.net)

These changes were characterized by an alteration of nuclear morphology and mitochondrial degeneration consistent with apoptotic cell death. (lsu.edu)

The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. (mybio.ie)
In the THP1 cell line, pri-let-7f levels were increased in response to LPS while the corresponding mature miRNA let-7f levels were reduced. (encyclopedia.pub)
Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)
Additional analysis of cytotoxicity mediated by MIH 2.4Bl was performed using the MCF-7 cell line. (lsu.edu)
Problematic cell line: Contaminated. (cellosaurus.org)
Parent cell line (VM7Luc4E2) shown to be a MCF-7 derivative. (cellosaurus.org)
Zygosity=Homozygous (from parent cell line). (cellosaurus.org)

Symmetric cell division prompts multiplication and asymmetric cell division is an enlightening advance for differentiation. (researchsquare.com)

Anatomy21

Organisms9

Diseases17

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes40

Disciplines and Occupations1

Information Science3

Health Care2

Receptors16

Proliferation17

Inhibition8

Apoptosis11

Epithelial cells2

Inhibiting estrogen receptor1

Metastatic breast cancer2

MRNA5

Cancers11

Estradiol5

Tumors8

Vitro11

Genes9

Heat-shock prot1

Regulate2

Protein9

Phenotype1

Metastasis3

Assay5

Expression17

Endogenous1

Effect of estrogen2

Receptor family1

Pathways5

Viability1

Transcription1

Pathway1

Migration and invasion1

Vivo3

Hepatic1

Binds1

Ovarian3

Compounds1

Tumor cells1

Resistance in breast cancer1

Cancer24

Concentrations3

Malignant1

EREs1

Progression1

Malignancy2

Survival1

Selective2

Mediates1

Cytotoxic effect1

Localization1

Apoptotic cell1

Line7

Differentiation1