Overexpressed in Escherichia coliEnzymesSubunitEnzymeBiosynthesisColi K-12CitrateSynthasePectate lyaseIsocitrate lyaseBacteriaStrainMutationPseudomonasClassificationPYRIDOXAL PHOSPHATEDehydrogenasePromoterIsomeraseHydrolaseOGG1Contributes1990CrystallographySequencePutativeSynthesisStructurallySynthetaseResidueReductaseConcentrationsUniProtKBMethionineMembranePyruvateMolecularAdditionallyStructuresHomologuesOperonGenes involvedFamilyAmino
Overexpressed in Escherichia coli1
- Two proteins (gbs1892 and gbs1891) of Streptococcus agalactiae strain NEM316 were overexpressed in Escherichia coli, purified, and characterized. (rcsb.org)
Enzymes9
- 2019 ). Structure-guided protein engineering has replaced the natural mutation of enzymes as one of the main tools of evolution (Cheng et al. (springeropen.com)
- This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. (antibodies-online.com)
- protein_coding" "AAC74185","ptsG","Escherichia coli","fused glucose-specific PTS enzymes: IIB component/IIC component [Ensembl]. (ntu.edu.sg)
- Whereas bacteria such as Escherichia coli have been known for some time to cleave carbon-phosphorus (C-P) bonds in unactivated alkylphosphonates, the enzymes responsible for C-P lyase activity have resisted detection or purification. (embl-heidelberg.de)
- 2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans - Characterization and comparison of both enzymes. (mpg.de)
- Fe-S clusters are present in more than 300 different types of enzymes or proteins and play crucial roles in fundamental life processes such as respiration, photosynthesis and nitrogen fixation. (uga.edu)
- In some organisms this enzyme is part of a multifunctional protein that also includes one or more of the enzymes EC 2.4.2.18 (anthranilate phosphoribosyltransferase), EC 4.1.1.48 (indole-3-glycerol-phosphate synthase), EC 4.1.3.27 (anthranilate synthase) and EC 5.3.1.24 (phosphoribosylanthranilate isomerase). (enzyme-database.org)
- A citrate utilization test helps detect the organism's ability to produce citrase enzymes, i.e., citrate lyase. (studyread.com)
- Cytochrome P450 enzymes (P450s or CYPs) are a superfamily of heme-thiolate proteins widespread in all kingdoms of life 1 . (nature.com)
Subunit4
- Citrate lyase subunit beta OS=Leuconostoc mesenteroides subsp. (uu.nl)
- Experimentally, we are involved, in collaboration with the Glockshuber group (ETHZ), in structural studies of a proteinaceous organelle, the type 1 pilus from uropathogenic Escherichia coli (UPEC), and in particular of its main subunit FimA. (psi.ch)
- In thermophilic organisms, where the high temperature enhances diffusion and causes the loss of indole, a protein similar to the β subunit can be found ( EC 4.2.1.122 ). (enzyme-database.org)
- Another lyase specific for a β-subunit of a phycobiliprotein was found by Shen et al. (biomedcentral.com)
Enzyme13
- Isocitrate lyase (EC 4.1.3.1) is an enzyme that catalyzes the conversion of isocitrate to succinate and glyoxylate. (wikipedia.org)
- The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(α-thyminyl)-5,6-dihydrothymidine. (cipsm.de)
- protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
- Isocitrate lyase (ICL), the first enzyme of the glyoxylate cycle, redirects isocitrate from the tricarboxylic acid cycle to render glyoxylate and succinate. (bvsalud.org)
- The His6-tag fusion at the C-ICL protein produced a less efficient enzyme than fusion at the N-ICL, but without affecting the acetate assimilation or virulence. (bvsalud.org)
- Cloning of the phosphonoacetate hydrolase gene from Pseudomonas fluorescens 23F encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression in Escherichia coli and Pseudomonas putida. (embl-heidelberg.de)
- The phnA gene encoding a novel carbon-phosphorus bond cleavage enzyme, phosphonoacetate hydrolase, from Pseudomonas fluorescens 23F was cloned and expressed in Escherichia coli and Pseudomonas putida. (embl-heidelberg.de)
- The T. vaginalis and P. putida alpha,gamma-lyases readily separate on a phenyl-Sepharose 6 FF column with the T. vaginalis enzyme eluting first. (unipr.it)
- To resolve this discrepancy, the enzyme encoded by butA Cg was produced in Escherichia coli and purified, and the stereospecific properties of the pure protein were examined. (uni-stuttgart.de)
- The extent of production is 25-fold higher than in chloroplast from spinach leaves and the recombinant protein presents the relative molecular mass and immunological properties of the natural enzyme from spinach leaf chloroplast. (unipr.it)
- The molecular mechanisms by which PA may exert this plethora of physiological effects are slowly being elucidated and include intestinal cyclooxygenase enzyme, the G-protein coupled receptors 41 and 43 and activation of the peroxisome proliferator-activated receptor γ, in turn inhibiting the sentinel transcription factor NF-κB and thus increasing the threshold for inflammatory responses in general. (ncats.io)
- This protein is a DNA glycosylase enzyme involved in base excision repair. (biofargo.com)
- The organism that grows and develops on this medium produces an enzyme called citrate permease (citrate transport protein), which has the ability to break citrate to oxaloacetate and acetate by the enzyme citrate lyase. (studyread.com)
Biosynthesis2
- The last enzymatic step for L-cysteine biosynthesis is catalyzed by O- acetylserine(thiol)lyase (OASTL, EC 4.2.99.8) which synthesizes L- cysteine from O-acetylserine and "sulfide. (unipr.it)
- Research on Fe-S cluster biosynthesis in the Johnson group currently involves investigating the molecular mechanisms of Fe-S cluster assembly on scaffold proteins and the subsequent intact cluster transfer to acceptor or carrier proteins. (uga.edu)
Coli K-121
- Escherichia coli K-12 substr. (lbl.gov)
Citrate2
- Citrate also increases the calcium oxalate aggregation inhibitory activity of urine macromolecules (eg, Tamm-Horsfall protein) and may reduce the expression of urinary osteopontin, which is an important component of the protein matrix of urinary stones. (medscape.com)
- The use of high-protein, low-carbohydrate diets for weight loss has led to concern about increased risk of stone formation, as these diets have been shown to be associated with decreased urinary citrate and pH levels and increased urine calcium and sodium levels in the induction and maintenance phases. (medscape.com)
Synthase5
- Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins with a specific Grade. (antibodies-online.com)
- Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins for a variety of species such as anti-Human N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Xenopus laevis N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase), anti-Cow N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase). (antibodies-online.com)
- Find N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) Proteins validated for a specific application such as ELISA, WB, SDS, AP. (antibodies-online.com)
- A geraniol synthase-LIS hybrid gene was constructed and expressed in Escherichia coli, and the protein catalyzed the formation of both geraniol and (R)-linalool from geranyl diphosphate. (myspecies.info)
- The total amounts of terpenes were correlated with total levels of terpene synthase activities, and negatively correlated with levels of phenylpropanoids and phenylalanine ammonia lyase activity. (myspecies.info)
Pectate lyase3
- His work with Pseudomonas also led to the cloning of pectate lyase genes and their insertion into Escherichia coli , converting a nonpathogen into a soft-rotting plant pathogen. (apsnet.org)
- Further collaborative work with X-ray crystallographers led to the discovery that the pectate lyase structure contained a new folding of the protein, the first discovered since the description of the alpha-helix and beta-sheets. (apsnet.org)
- Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. (biomedcentral.com)
Isocitrate lyase3
- Isocitrate lyase family is a family of evolutionarily related proteins. (wikipedia.org)
- Isocitrate lyase InterPro: IPR006254 Methylisocitrate lyase InterPro: IPR012695 Carboxyvinyl-carboxyphosphonate phosphorylmutase InterPro: IPR012697 Beeching JR (1989). (wikipedia.org)
- The domains are named after Escherichia coli iclR (isocitrate lyase regulator), a repressor of the glyoxylate bypass operon for acetate utilization. (expasy.org)
Bacteria4
- Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
- The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
- Genes coding for glycosaminoglycan-acting lyase, unsaturated glucuronyl hydrolase, and the phosphotransferase system are assembled into a cluster in the genome of pathogenic bacteria, such as streptococci and clostridia. (rcsb.org)
- The 181-amino-acid sequence of NbaB is 35% identical to the 4-hydroxylaminobenzoate lyases (PnbBs) of various 4-nitrobenzoate-assimilating bacteria, e.g. (canada.ca)
Strain1
- The ICL-mutant A39 complemented with the recombinant proteins N-His6-ICL or C-His6-ICL were more virulent than the WT PAO1 strain. (bvsalud.org)
Mutation1
- While the recombinant wild type MsmNDK showed the NTPase, NTP synthesising, and autophosphorylation activities, the H117Q mutation abolished the biochemical activities of the recombinant MsmNDK-H117Q mutant protein in vitro . (openbiochemistryjournal.com)
Pseudomonas1
- The Trichomonas rHCYase is selective for homocysteine with respect to very low cysteinase activity in contrast to the alpha,gamma-lyase from Pseudomonas putida, which has very high cysteinase activity with respect to homocysteine. (unipr.it)
Classification1
- 2010). In a second phase we used two sequence entropy-based evolutionary indicators and a simple geometric criterion to obtain accurate and efficient classification of protein-protein interfaces (Duarte et al. (psi.ch)
PYRIDOXAL PHOSPHATE1
- A pyridoxal-phosphate protein. (enzyme-database.org)
Dehydrogenase1
- protein_coding" "AAC74323","adhE","Escherichia coli","fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase [Ensembl]. (ntu.edu.sg)
Promoter1
- Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. (unipr.it)
Isomerase3
- The AceA/ICL protein family shares the catalytic domain I, triosephosphate isomerase barrel (TIM-barrel). (bvsalud.org)
- Phosphomannose isomerase from Escherichia coli is a high quality recombinant Phosphomannose isomerase (Escherichia coli). (moleculardepot.com)
- L-Ribose production from L-arabinose by immobilized recombinant Escherichia coli co-expressing the L-arabinose isomerase and mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans Appl Biochem Biotechnol. (moleculardepot.com)
Hydrolase2
- Glycosaminoglycans in mammalian extracellular matrices are degraded to their constituents, unsaturated uronic (glucuronic/iduronic) acids and amino sugars, through successive reactions of bacterial polysaccharide lyase and unsaturated glucuronyl hydrolase. (rcsb.org)
- This protein is not related to the characterised phosphonoacetate hydrolase designated PhnA. (embl-heidelberg.de)
OGG11
- Additionally, OGG1 has a beta lyase activity that nicks DNA 3' to the lesion. (biofargo.com)
Contributes1
- PCC 6803 slr 1649-encoded protein also acts as a bilin lyase, and additionally contributes to linker attachment and/or stability of phycobilisomes. (biomedcentral.com)
19901
- Para estos valores de dosis, las estimaciones del riesgo de segundos tumores en los supervivientes a largo plazo indican una reducida probabilidad de contraer una segunda malignidad por radiación secundaria, según la información del informe del Comité BEIR de 1990. (bvsalud.org)
Crystallography1
- We also collaborate with other groups for Mossbauer, electron-nuclear double resonance (ENDOR), X-ray absorption and X-ray crystallography studies of proteins prepared in our laboratory. (uga.edu)
Sequence4
- Sequence homology searches identify PhnC, PhnK, PhnL, and, possibly, PhnN proteins as members of nucleotide-binding proteins of the binding protein-dependent transport systems. (embl-heidelberg.de)
- The derived amino acid sequence indicates that the protein precursor consists of 383 amino acid residues including a N-terminal presequence peptide of 52 residues. (unipr.it)
- The amino acid sequence of mature spinach chloroplast O- acetylserine(thiol)lyase shows 40 and 57% homology with its bacterial counterparts. (unipr.it)
- Sequence comparison with several pyridoxal 5'-phosphate- containing proteins reveals the presence of a lysine residue assumed to be involved in cofactor binding. (unipr.it)
Putative1
- Analyses of the proteins encoded within this region by ΦSH19 revealed a cluster of three putative tail spikes. (biomedcentral.com)
Synthesis2
- Synthesis and protein binding of (4-carboxybutyl)carbamoyl- substituted taxoids. (mpg.de)
- Effects of butyrate and propionate on the adhesion, growth, cell cycle kinetics, and protein synthesis of cultured human gingival fibroblasts. (ncats.io)
Structurally1
- Herein, we report a group of variants structurally derived from Escherichia coli O157:H7 MAL ( Ec MAL). (springeropen.com)
Synthetase1
- Crawford, I.P. and Yanofsky, C. On the separation of the tryptophan synthetase of Escherichia coli into two protein components. (enzyme-database.org)
Residue1
- E. Coli Nifs/csdb Protein at 2.0a with The Cysteine Persulfide Intermediate (residue Css). (me.uk)
Reductase2
- Enoyl-(Acyl carrier protein) reductase [Interproscan]. (ntu.edu.sg)
- There are two major P450 classes in terms of the native RP systems, namely, the prokaryotic Class I P450 consisting of three stand-alone components (redoxin reductase/redoxin/P450) that are all cytosolically soluble proteins and the two-component eukaryotic Class II P450 comprised of P450 and cytochrome P450 reductase (CPR), both of which are membrane-bound proteins. (nature.com)
Concentrations1
- N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. (antibodies-online.com)
UniProtKB1
- Search proteins in UniProtKB for this molecule. (rhea-db.org)
Methionine1
- A synthetic cDNA was constructed, coding for the entire 331-amino-acid mature O-acetylserine(thiol)lyase and for an initiating methionine. (unipr.it)
Membrane3
- Furthermore, plasma membrane proteins and intracellular proteins can be released into the extracellular space by regulated or non-regulated processes. (cipsm.de)
- Candidates for other membrane components and regulatory proteins are also identified. (embl-heidelberg.de)
- Allophycocyanin (AP, λ max = 650 nm) forms the core structure, connecting the phycobilisomes to the thylakoid membrane via linker proteins. (biomedcentral.com)
Pyruvate2
- pyruvate formate-lyase 1 [Ensembl]. (ntu.edu.sg)
- Glycine radical, Pyruvate formate lyase-like [Interproscan]. (ntu.edu.sg)
Molecular3
- Small heat shock proteins (sHsps) are molecular chaperones that prevent the aggregation of nonnative proteins. (cipsm.de)
- We are studying protein structures and crystal lattices by employing a variety of approaches, in particular concepts from the field of molecular evolution. (psi.ch)
- Moreover, a molecular-level understanding of iron-sulfur cluster biogenesis is crucial for understanding a variety of human diseases involving anemias, myopathies and ataxias that arise from defects in Fe-S cluster biogenesis proteins. (uga.edu)
Additionally1
- Additionally, we found that the BDH activity was nearly abolished upon inactivation of butA Cg (from 0.30 ± 0.03 to 0.004 ± 0.001 μmol min-1 mg protein-1), indicating that C. glutamicum expresses a single BDH under the experimental conditions examined. (uni-stuttgart.de)
Structures4
- He was a prolific crystallographer, with 70 protein structures in the Protein Data Bank (PDB) over the past two decades. (psi.ch)
- From there, he worked with Markus Grütter at the University of Zurich, elucidating the structures of numerous proteins. (psi.ch)
- He continued to solve protein structures at the Swiss Light Source, and also put forward a program of large-scale bioinformatic analysis of structures. (psi.ch)
- Tail spike proteins of Vi01 and SboM-AG3 are predicted to contain conserved right-handed parallel β-helical structures but the internal protein domains are varied allowing different host specificities. (biomedcentral.com)
Homologues1
- The PhnA protein family includes the uncharacterised Escherichia coli protein PhnA and its homologues. (embl-heidelberg.de)
Operon3
- This protein family includes an uncharacterised member designated phnA in Escherichia coli, part of a large operon associated with alkylphosphonate uptake and carbon-phosphorus bond cleavage. (embl-heidelberg.de)
- Escherichia coli iclR, a repressor of the aceBAK operon (also known as glyoxylate bypass operon) for acetate utilization. (expasy.org)
- Escherichia coli mhpR, an activator of the mhpRABCDEF operon coding for components of the 3-hydroxyphenylpropionate degradation pathway. (expasy.org)
Genes involved1
- Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. (embl-heidelberg.de)
Family1
- The addition or exchange of tail spike protein modules is a major contributor to host range determination in the Vi01-like phage family. (biomedcentral.com)
Amino1
- Functional Replacement of Histidine in Proteins To Generate Noncanonical Amino Acid Dependent Organisms J Am Chem Soc. (moleculardepot.com)