Escherichia microbial genes bacterial. Medical search. Frequent questions

The traditional metagenomic screening approach for antibiotic resistance genes (ARGs) relies on laboratory strains of Escherichia coli as bacterial hosts, leaving us blind to ARGs that provide resistance specifically in clinically relevant pathogens. (brc.hu)
Employing an exciting array of transgenic animals, pathogenic and commensal bacterial strains, andhuman intestinal organoids, three specific aims are proposed that will (i) investigate metabolite-dependentregulation of enteric infection, (ii) decipher how the host calibrates intestinal barrier function by sensing distinctcommensal bacterial-derived metabolites, and (iii) interrogate whether distinct types of commensal bacteriaprime the epigenome to enhance host response to pathogenic bacteria. (usda.gov)
Both phages were extensively tested on many strains of Escherichia coli and other enterobacteria. (cdc.gov)
These controls are prepared as whole cell or nucleic acid mixtures comprising three genetically engineered bacterial strains (derived from Escherichia coli , Staphylococcus aureus , and Clostridium perfringens ), each containing a unique synthetic DNA tag that can be detected and quantified in routine 16S rRNA gene amplicon and shotgun sequencing assays. (atcc.org)
To demonstrate the utility of these spike-in controls in microbiome research, we conducted studies where we mixed them with whole-cell or gDNA mock communities containing different bacterial strains at various ratios. (atcc.org)
The O antigen cluster of a Salmonella serogroup O41 isolate had a lower level of identity with E. coli O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. (microbiologyresearch.org)
We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
Sub-objective 4.1: Analysis of ExPEC isolated from chickens and humans: biofilm assays, virulence gene profiles, antimicrobial resistance profiles, whole genome comparison of ExPEC strains isolated from chicken and human infections. (usda.gov)
We will use omic technologies to analyze a large variety of strains of each of the pathogens to identify genes and proteins necessary for pathogens to survive stresses encountered in food environments and cause human illness. (usda.gov)
The rise of antibiotic-resistant bacterial strains, however, represents a serious threat to public health and the economy. (cdc.gov)

Our results show that although pOXA-48_K8 produced an overall reduction in bacterial fitness, it produced small effects in most bacterial hosts, and even beneficial effects in several isolates. (nature.com)
MDR plasmid transfer from Salmonella to commensal Escherichia coli was demonstrated by PCR and whole-genome sequencing of isolates purified from agar plates containing cefotaxime. (nih.gov)
Anti- and mortality among children in developing microbial resistance among clinical isolates countries [1]. (who.int)
Therefore, in our study, E. coli complex, multi-factorial mechanism involv- isolates from diarrhoeal cases were ana- ing a large number of virulence factors that lysed using specific DNA probes for genes vary with pathotype. (who.int)
13.0%) isolates carried genes encoding 3 tion. (who.int)
Isolates were screened for presence different toxins and 2 (2.2%) isolates were of genes encoding LT and ST enterotoxins, positive with all 4 DNA probes. (who.int)
Comparative genomics of ESBL-producing Escherichia coli (ESBL-Ec) reveals a similar distribution of the 10 most prevalent ESBL-Ec clones and ESBL genes among human community faecal and extra-intestinal infection isolates in the Netherlands (2014-17). (cdc.gov)
Three S. enterica isolates had O antigen gene clusters with homology to the Escherichia coli O123 O antigen region. (microbiologyresearch.org)
The E. coli O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for E. coli O123 was entirely consistent with the O antigen gene cluster sequences of E. coli O123 and the Salmonella O58 isolates. (microbiologyresearch.org)
Most studies addressing travel-related changes in the faecal flora have focused on specific mobile resistance genes, or depended on culturing of individual bacterial isolates. (biomedcentral.com)
The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. (uni-wuerzburg.de)
Culturomics-based bacterial diversity studies benefit from the implementation of MALDI-TOF MS to remove genomically redundant isolates from isolate collections. (mdpi.com)
This study was aimed at determining the urinary bacterial isolates and their susceptibility to selected antibiotics among pregnant women attending antenatal clinics of selected Hospitals in Ilorin, Nigeria. (who.int)
Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
Four of the 9 (44.4%) CoNS isolates were cefoxitin resistant and all the 4 (100%) carried mec A gene. (who.int)
Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

To address this issue, DEEPMINE employs modified bacteriophage transducing particles to deliver large metagenomic plasmid libraries into a wide range of bacterial species, allowing us to perform metagenomic screens in clinically relevant bacterial pathogens from the Enterobacteriaceae family. (brc.hu)
However, despite continuous exposure to diverse microbes, the mechanisms regulating howIECs integrate microbial-derived signals to mount protective host responses to pathogens are not wellunderstood. (usda.gov)
Finally, we discuss the opportunities and challenges of autophagy manipulation in improving therapeutics and vaccines against bacterial pathogens. (frontiersin.org)
Here, we review the many functions of autophagy in bacterial infections with a focus on macrophages, the first line of host defenses, and the replicative niche of numerous pathogens. (frontiersin.org)
The antimicrobial activity of the crude acetone leaf extracts was determined against six common nosocomial pathogens, namely: Gram-positive bacteria ( Bacillus cereus , Enterococcus faecalis , Staphylococcus aureus ), Gram-negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Salmonella Typhimurium) using a two-fold serial microdilution assay with p-iodonitrotetrazolium violet as growth indicator. (biomedcentral.com)
Pathogens that will be investigated include: Shiga toxin-producing Escherichia coli (STEC) and extraintestinal pathogenic E. coli (ExPEC), Campylobacter species, and Listeria monocytogenes. (usda.gov)
The isolation of bacterial pathogens resistant to the commonly prescribed antibiotics from pregnant women symptomatic and asymptomatic for UTI calls for early screening of all pregnant women for UTI during antenatal care service delivery. (who.int)
Complete genomic sequences of microbial pathogens and hosts offer sophisticated new strategies for studying host-pathogen interactions. (medscape.com)

The gene rpoE (RNA polymerase, extracytoplasmic E) encodes the sigma factor sigma-24 (σ24, sigma E, or RpoE), a protein in Escherichia coli and other species of bacteria. (wikipedia.org)
Native Escherichia coli bacteria isolated from the stool cultures of CR mice were modified to express functional genes. (nih.gov)
Thus, using native bacteria as chassis to "knock in" specific functions allows mechanistic studies of specific microbial activities in the microbiome of CR hosts and enables LBT with curative intent. (nih.gov)
It can also facilitate the conjugative transfer of multidrug resistance (MDR) plasmids between commensal and pathogenic bacteria which is a significant public and animal health concern as it may affect our ability to treat bacterial infections. (nih.gov)
New preliminary data suggest commensal bacterial-derived metabolites candirectly modulate HDAC3 function in IECs and that distinct types of commensal bacteria establish uniquehistone acetylation signatures in IECs. (usda.gov)
In the present study, the various extracts of Morinda Pubescens bark was tested for their antibacterial and antifungal activities against gram positive bacteria such as Staphylococcus aureus, Bacillus cereus, gram negative organisms such as Escherichia coli, Pseudomonas aeruginosa and fungi such as Candida albicans, Aspergillus niger respectively by cup plate method. (ijpsr.com)
International travel can alter the composition of the Escherichia communities in the faecal flora, favouring bacteria carrying certain resistance mutations, and, thereby, contributes to the global spread of antibiotic resistance. (biomedcentral.com)
Bacteria can acquire resistance by gaining mobile antibiotic resistance genes or by de novo mutations in pre-existing DNA. (biomedcentral.com)
Consequently, bacteria have developed different systems to sense changes in environmental conditions such as temperature and induce an adaptation of metabolism and gene expression. (springer.com)
Evidence is growing that extensive horizontal transfer of antibiotic resistance genes occur in nature between clinical and nonclinical bacteria [2]. (helpmyessay.com)
Hence the commensal reservoir bacteria may be important players in the spread of antibiotic resistance genes. (helpmyessay.com)
Experiments would measure the efficiency of gene transfer between different classes of bacteria upon exposure of triclosan. (helpmyessay.com)
The efficiency of transformation by naked DNA and gene transfer between bacteria vial bacterial conjugation can be examined upon exposure to various levels of triclosan. (helpmyessay.com)
Furthermore, our group also expected to see inhibited bacterial growth in the LB/amp +pGLO dish as there was ampicillin in the dish, but some of the bacteria were immune as they possessed immunity to the ampicillin. (bartleby.com)
Moreover, our group expected that there would be no bacterial growth in the LB/amp -pGLO dish, as the bacteria were exposed to ampicillin and were not immune. (bartleby.com)
1993). Accordingly, specific LF-binding microbial targets exist in a variety of Gram-positive and Gram-negative bacteria (Naidu et al. (ift.org)
The interaction of LF with microbial surfaces-OMPs of Gram-negative bacteria in particular-has led to other antimicrobial mechanisms, such as the inhibition of microbial attachment to sub-epithelial matrix proteins and detachment of bacteria from mucosal surfaces. (ift.org)
CDC) estimates that annually, at least two million illnesses and 23,000 deaths are caused by antibiotic-resistant bacteria in the United States alone.1 If the effectiveness of antibiotics (drugs that kill or inhibit the growth of bacteria) is lost, we will no longer be able to reliably and rapidly treat bacterial infections, including bacterial pneumonias, foodborne illnesses, and healthcare- associated infections. (cdc.gov)
After biomechanical preparation of root canals, the bacterial load is reduced by at least 95% and the remaining viable bacteria may be the cause of refractory lesions 8 . (bvsalud.org)

However, attempts to functionally manipulate the gut microbiome of conventionally raised (CR) hosts have been unsuccessful because engineered microbial organisms (i.e., chassis) have difficulty in colonizing the hostile luminal environment. (nih.gov)
Further, the natural complexity of microbial communities themselves has challenged microbiome researchers in their ability to make meaningful, quantifiable, reproducible, and comparable measurements across different laboratories. (atcc.org)
This mock microbial community can be used in the optimization of metagenomics workflows and microbiome research applications, improving the consistency of data run after run. (atcc.org)
To understand the relationship between our bacterial microbiome and health, it is essential to define the microbiome in the absence of disease. (biomedcentral.com)
We describe the bacterial community composition of ten digestive tract sites from more than 200 normal adults enrolled in the Human Microbiome Project, and metagenomically determined metabolic potentials of four representative sites. (biomedcentral.com)
The bacterial microbiome of the human digestive tract contributes to both health and disease. (biomedcentral.com)
We have several collaborations with experimental and clinical laboratories in order to analyse the human microbiome data at different taxonomic levels as well as functional potentials between diverse microbial communities for understanding human health and diseases that are associated with the microbiota. (jisiasr.org)
and (2) that the development of host-free microbiome model systems could be useful for rapidly screening pesticides against functionally distinct microbial catalogues of interest. (researchgate.net)

These results help to explain the high prevalence of plasmids in the greatly diverse natural microbial communities. (nature.com)
Some of the most clinically relevant resistance genes, such those encoding carbapenemases (ß-lactamase enzymes able to degrade carbapenem antibiotics), are carried on conjugative plasmids that spread across high-risk bacterial clones 5 , 6 . (nature.com)
These fitness costs make it difficult to explain how plasmids are maintained in bacterial populations over the long-term in the absence of selection for plasmid-encoded traits, a puzzle known as "the plasmid-paradox" 10 . (nature.com)
Plasmids carrying marker genes such as those coding for tetracycline and kanamycin resistance will be introduced into several hosts (Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas aeruginosa). (helpmyessay.com)

Intestinal tissues are inconstant direct contact with diverse beneficial and pathogenic microbes, highlighting the need for orchestratingcomplex microbial signals to sustain protection against infection. (usda.gov)
Defining pathways that integratecommensal and pathogenic signals will provide a framework to test the therapeutic potential of manipulatingcommensal bacterial-derived metabolites to promote antibacterial immunity. (usda.gov)
For this purpose, the non-pathogenic production host P. putida KT2440 containing the rhlAB genes with the native P. aeruginosa 5′-UTR region was used. (springer.com)
Objective 4: Phenotypic and genetic characterization of extra-intestinal pathogenic Escherichia coli (ExPEC) isolated from poultry and produce. (usda.gov)

The most frequent target of resistance mutations was the plasmid-encoded SpCas9 that was inactivated by bacterial genome rearrangements involving translocation of mobile genetic elements such as insertion elements. (dtu.dk)
A common protocol for the simultaneous processing of multiple clinically relevant bacterial species for whole genome sequencing. (cdc.gov)
E. coli has numerous representatives of genes present in the oldest living ancestor genome as deduced by these methods. (cns.fr)
The design and construction of a DNA microarray for any given microbial genome are straightforward. (medscape.com)

The goal of this project is to explore the relative contributions of various evolutionary processes shaping the host-associated microbial community leading to phylosymbiosis and thus further better understanding of the ecology and evolution of host-microbe interactions. (jisiasr.org)
His interests are in the development and use of molecular methods for pathogen discovery, human microbial ecology, genomewide host and microbial responses to infection, and Bordetella pathogenesis. (medscape.com)

These thermosensors are part of a regulatory network, such as the production of heat shock proteins mediated by sigma factor 32 (RpoH) in Escherichia coli or the transcriptional repressor of heat-shock genes HrcA in Bacillus subtilis (Hecker et al. (springer.com)
To find the homologs, the tool analyses the similarity results between the genes of each organism and the set of 206 genes from 7 genomes (Escherichia coli K12, Bacillus subtilis 168, Candidatus Blochmania floridanus, Buchnera aphidicola APS, Buchnera aphidicola Bp, Buchnera aphidicola Sg and Mycoplasma genitalium G37). (cns.fr)

The Cardiff University professor, who reported on the enzyme called New Delhi metallo beta lactamase, says China and Pakistan are more serious about anti-microbial resistance genes than India. (thehindu.com)
We'd hoped that the government would then set in place a surveillance system for tracking anti-microbial resistance genes. (thehindu.com)
Are there only certain resistance genes to be worried about or is the problem more widespread? (thehindu.com)
It would be incredibly hard to find all the sources of resistance genes. (thehindu.com)
If resistance genes are all around, how worried should we be? (thehindu.com)
You and I are chock-full of resistance genes. (thehindu.com)
Since triclosan disrupts the microbial cell membrane, it is important to examine whether triclosan affects the acquisition of antibiotic resistance genes. (helpmyessay.com)

Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. (bartleby.com)
The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase. (microbiologyresearch.org)
The MPO gene encodes for a primary translational product, which is glycosylated to yield an enzymatically inactive precursor, apopro-MPO. (medscape.com)

IMPORTANCE The spread of antimicrobial resistance presents a grave threat to public health and animal health and is affecting our ability to respond to bacterial infections. (nih.gov)
In our recent publication in Nature Microbiology , we introduced a new approach called Reprogrammed Bacteriophage Particle Assisted Multi-species Functional Metagenomics (DEEPMINE) to expand the functional metagenomic toolbox for studying microbial communities and by doing so, deepen our understanding of how antimicrobial resistance emerges. (brc.hu)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

Here we use the chicken gut microbiota as an exemplar to model the effects of bacterial infection, antibiotic administration, and plasmid transfer. (nih.gov)
PROJECT SUMMARYEnteric bacterial infections remain one of the greatest public health challenges worldwide and deciphering themechanisms that protect against infection will enable development of new treatments. (usda.gov)
The goals of this proposal are to interrogate how specific commensalbacterial-derived metabolites that regulate the epigenetic-modifying enzyme histone deacetylase 3 (HDAC3)influence intestinal protection against infection and bacterial translocation. (usda.gov)
Employing Citrobacter rodentium, amurine model of human enteropathogenic Escherichia coli infection, our studies identified that HDAC3 protectsagainst enteric bacterial infection. (usda.gov)
Jag är medlem i forskarkonsortiet Umeå Centre for Microbial Research (UCMR) som utgör den vetenskapliga miljön där The Laboratory for Molecular Infection Medicine Sweden (MIMS) etablerades. (umu.se)
Likewise the ability of triclosan to inhibit bacteriophage infection, another common method of gene transfer will be analysed. (helpmyessay.com)
Similarly, by using host gene microarrays, one can explore host response at the level of gene expression and provide a molecular description of the events that follow infection. (medscape.com)

Moreover, genomic results showed a link between pOXA-48_K8 fitness effects and bacterial phylogeny, helping to explain plasmid epidemiology. (nature.com)
Ten years of population-level genomic Escherichia coli and Klebsiella pneumoniae serotype surveillance informs vaccine development for invasive infections. (cdc.gov)
The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
This functionalty provides the list of Genomic regions containg tandem duplications of protein coding genes. (cns.fr)

In order to perform studies of such design and test if previously defined quantitative data about the functioning of microorganisms (i.e., protein-to-RNA ratio) is applicable to real world consortia, we must first sample microbial communities across transition events and employ quantification techniques that are absolute. (nature.com)
Transcriptional regulation of the genes in metabolic pathways is a highly successful strategy, which is virtually universal in microorganisms. (lu.se)
A number of studies have explored how regulation of generally, and at least to a first approximation, it is obvious that metabolic pathways affects the growth rate of microorganisms, gene regulation only is useful if the environmental conditions vary both in the steady state and in response to changes in the local with time. (lu.se)

For these studies, we have developed a new functional metagenomic method for studying horizontal gene transfer in microbial communities with the help of engineered bacteriophages. (brc.hu)

It is these prebiotic and anti-microbial actions of HMOs that make them highly desirable ingredients for infant formula. (milkgenomics.org)

We used an in vitro chemostat system to approximate the chicken cecal microbiota, simulate colonization by an MDR Salmonella pathogen, and examine the dynamics of transfer of its MDR plasmid harboring several genes, including the extended-spectrum beta-lactamase bla CTX-M1 We also evaluated the impact of cefotaxime administration on plasmid transfer and microbial diversity. (nih.gov)
While the impact of inactivated viral vaccines and live-attenuated bacterial vaccines on the host microbiota have been examined, the potential impact of using subunit vaccines consisting of antigens that are also encoded by commensal organisms has not been investigated. (biomedcentral.com)

A comprehensive and high-quality collection of Escherichia coli genomes and their genes. (cdc.gov)
The minimal set of genes obtained is a function of which and how many genomes are chosen for the analysis. (cns.fr)
Several independent high-throughput methods for differential gene expression (including SAGE and differential display) may enable function annotation of sequenced genomes [ 2 ] . (medscape.com)

These metabolites are believed to play a role in microbial defense. (joessciencescorner.net)
thus, the MPO-hydrogen peroxide-Cl system seems to have an important role in microbial killing. (medscape.com)

However, plasmid fitness effects in wild-type bacterial hosts remain largely unexplored. (nature.com)
These examples do not necessarily replicate plasmid fitness effects in natural bacterial hosts, which remain largely unexplored. (nature.com)
In this study, we provide a detailed characterisation of the distribution of plasmid fitness effects in wild-type bacterial hosts. (nature.com)
The digestive tract includes diverse habitats and hosts the human body's greatest bacterial density. (biomedcentral.com)
This describes the host-associated similarities in the microbial communities that can even define the phylogeny of their hosts. (jisiasr.org)

We show that transfer of a multidrug-resistant plasmid from the zoonotic pathogen Salmonella to commensal Escherichia coli occurs at a high rate, even in the absence of antibiotic administration. (nih.gov)
The bacterial pathogen most is an important public health issue. (who.int)
Host profiling might also identify gene expression signatures unique for each pathogen, thus providing a novel tool for diagnosis, prognosis, and clinical management of infectious disease. (medscape.com)
The complex interaction between a microbial pathogen and a host is the underlying basis of infectious disease. (medscape.com)

We have a programme on neonatal sepsis (bacterial disease in newborns) in Kolkata. (thehindu.com)

While the field of microbiology has adapted to the study of complex microbiomes via modern meta-omics techniques, we have not updated our basic knowledge regarding the quantitative levels of DNA, RNA and protein molecules within a microbial cell, which ultimately control cellular function. (nature.com)

This undermines the effectiveness of the available treatment options and thus contributes to the persistence of microbial infections. (who.int)
Autophagy is a well-conserved lysosomal degradation pathway that plays key roles in bacterial infections. (frontiersin.org)
Several recent studies point to an even broader range of activity for MAIT cells including: the control of bacterial, fungal and viral infections, a role in autoimmune disease and possible involvement in the immune processes involved in attacking the proliferation of tumor cells. (joessciencescorner.net)
In cases of primary endodontic infections, there is a higher bacterial load than in secondary infections 6-7 . (bvsalud.org)

Plasmid persistence in bacterial populations is strongly influenced by the fitness effects associated with plasmid carriage. (nature.com)
To fully understand plasmid persistence in natural bacterial populations, it will be necessary to address these limitations. (nature.com)
Our analysis of this data reveals an absolute protein-to-RNA ratio of 10 2 -10 4 for bacterial populations and 10 3 -10 5 for an archaeon, which is more comparable to Eukaryotic representatives' humans and yeast. (nature.com)
In this context, the exploration of temporal interplay between populations with different lifestyles (comprising metabolism, motility, sporulation, etc.) becomes of primary importance to interpret the changes in fundamental quantities in a microbial community, such as the protein-to-RNA ratio that ultimately impacts the overarching community phenotype(s). (nature.com)
Indeed, the protein-to-RNA ratio per sample of the bacterial populations matched previous calculations for the existing example from axenically cultured E. coli 2 . (nature.com)

Autophagy can further be regulated by several transcriptional factors such as NFkappaB and TFEB to promote expression of different autophagy genes and thus prolong autophagy activation ( 6 , 7 ). (frontiersin.org)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)
INTRODUCTION broken, which in realistic situations can severly constrain the Transcriptional regulation of effector genes is a highly successful regulatory options. (lu.se)

Although the phylosymbiosis has been described across several animal and plant systems, we lack in the understanding of the various evolutionary mechanisms shaping the host specific microbial community structure. (jisiasr.org)

The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
The high-affinity interaction of LF with pore-forming outer-membrane proteins (OMPs) of Gram-negative enterics, including Escherichia coli , is critical for the antimicrobial outcome of LF (Gado et al. (ift.org)

In comparison to the 40 organisms detectable by PCR, NGS analyzes all microbial DNA within a sample (eg, urine, semen, or vaginal or rectal specimen) and compares it to a database of species. (urologytimes.com)
Methods of DNA transfer between organisms include transformation by naked DNA, viral transduction, and bacterial conjugation. (helpmyessay.com)

2014. Transcriptome analysis of peripheral blood mononuclear cells in human subjects following a 36 h fast provides evidence of effects on genes regulating inflammation, apoptosis and energy metabolism . (hutton.ac.uk)
These are referred to as mucosal-associated invariant T (MAIT) cells and that these MAIT cells recognize and react to metabolites that are that are a byproduct of microbial metabolism. (joessciencescorner.net)
A very natural place to study gene detecting and metabolizing lactose, it is known that the overall regulation is in the metabolism of the cell, and then specifically in effect of expressing the lac genes in vain is a drop in the growth rate the regulation of genes that code for enzymes and transporter of as much as 5% [1,6]. (lu.se)

Endotoxin triggers humoral enzymatic mechanisms involving the complement, clotting, fibrinolytic, and kinin pathways and causes much of the morbidity in gram-negative bacterial sepsis. (msdmanuals.com)

Bacterial community profiles obtained by culture-independent methods showed that Salmonella inoculation resulted in no significant changes to bacterial community alpha diversity and beta diversity, whereas administration of cefotaxime caused significant alterations to both measures of diversity, which largely recovered. (nih.gov)
Subinhibitory concentrations of tetracycline affect virulence gene expression in a multi-resistant Salmonella enterica subsp. (mcmaster.ca)

Correlation between growth rates, EIIA Crr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12. (microbiologyresearch.org)
Intracellular events, such as blocking of microbial attachment factors such as fimbriae (hairlike structures) and other adhesins (putative receptors), have been observed (Naidu and Bidlack, 1998). (ift.org)

Incorporating our fitness results into a simple population dynamics model revealed a new set of conditions for plasmid stability in bacterial communities, with plasmid persistence increasing with bacterial diversity and becoming less dependent on conjugation. (nature.com)
This unique dataset permits novel studies of the human digestive tract within a large number of subjects, allows for comparisons of microbial communities between habitats, and enables the definition of distinct metabolic niches within and among individuals. (biomedcentral.com)
Here we aimed at exploring the abundance of chromosomal quinolone resistance mutations in Escherichia communities residing in the gut of Swedish travellers, and to determine potential changes after visiting India. (biomedcentral.com)
The advent of a new sequencing era like high-throughput DNA sequencing and metagenomic studies have explored the highly diverse and dynamic nature of the microbial communities and their crucial role in human health and in disease. (jisiasr.org)
Additionally, the development of MAIT cells depends upon exposure in early life to well-defined microbial communities. (joessciencescorner.net)

We have therefore sequenced additional S. enterica O antigen gene clusters to provide information for the development of DNA-based serotyping methods. (microbiologyresearch.org)
Historic definitions of microbial indicators, such as coliforms, have been tied to the methods used to measure them. (nationalacademies.org)
The course presents methods to express genes, which have products that are known, unknown or not well characterized, and to analyze the gene products. (lu.se)

The HMP in particular includes, for each sample, both 16S rRNA gene surveys and shotgun metagenomic sequences, from a subset of the subjects recruited at two geographically distant locations in the United States. (biomedcentral.com)

Depending on the bacterial species, this gene may be referred to as sigE. (wikipedia.org)
In some bacterial species, such as Clostridium botulinum, this sigma factor may be necessary for sporulation. (wikipedia.org)
The plant species examined in this study had varying degrees of antibacterial activity against bacterial planktonic and biofilm forms with some having good activity against both forms. (biomedcentral.com)
Even more astonishing, a recent study of their database found that Escherichia coli was the predominant species in 29.6% of samples, calling for further analysis of the belief that E. coli is the most common cause of UTI. (urologytimes.com)

DNA microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. (medscape.com)

Here we report the temporal measurements of absolute RNA and protein levels per gene within a mixed bacterial-archaeal consortium. (nature.com)
For example, when Escherichia coli is grown axenically in steady state, we can expect that each RNA molecule corresponds to 10 2 -10 4 of the matching protein (absolute protein-to-RNA ratio, hereafter referred protein-to-RNA ratio) and the variation in the level of cellular RNA explains ~29% of the variation in the amount of detectable protein 2 . (nature.com)

Live bacterial therapeutics (LBTs) could reverse diseases by engrafting in the gut and providing persistent beneficial functions in the host. (nih.gov)

Our focus on the alterations in the efficiencies of gene transfer mechanisms upon exposure to triclosan may elucidate novel physiological effects. (helpmyessay.com)
Mechanisms of bacterial biocide and antibiotic resistance . (helpmyessay.com)
By understanding the molecular details of this interaction, we can identify virulence-associated microbial genes and host-defense strategies and characterize the cues to which they respond and mechanisms by which they are regulated. (medscape.com)

This model also permits the quantification of mouse survival, bacterial clearance, and host immune responses, and stimulates mucosal immune responses, especially secretory IgA (sIgA) responses that are important to blocking bacterial adherence to mucosal surfaces. (biomedcentral.com)

During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. (bvsalud.org)

Subsequently, we did an extensive functional assessment of the candidate genes to explore their causality in IBD pathogenesis. (bmj.com)

Interaction of Escherichia coli DbpA with 23S rRNA in different functional states of the enzyme. (colorado.edu)

An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)

Objective 1: Characterization of environmental and food-related stress responses of Shiga-toxin producing Escherichia coli (STEC). (usda.gov)

The ability of the plant extracts to inhibit or destroy pre-formed bacterial biofilms was also determined. (biomedcentral.com)
Considered generally recognized as safe (GRAS) by the Food and Drug Administration and recently approved by the U.S. Dept. of Agriculture for use on fresh beef, ALF can be sprayed onto carcasses to help prevent bacterial contamination during processing or can be applied to a subprimal or finished beef surface prior to final packaging to inhibit bacterial growth and extend shelf life. (ift.org)

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. (microbiologyresearch.org)

Anatomy1

Organisms4

Diseases1

Chemicals and Drugs4

Phenomena and Processes9

Information Science4

Health Care1

Strains10

Isolates16

Pathogens8

Bacteria19

Microbiome8

Plasmids4

Pathogenic4

Genome4

Ecology2

Bacillus2

Resistance genes7

Encodes3

Antimicrobial resistance3

Infection7

Genomic4

Microorganisms3

Horizontal gene tra1

Anti-microbial1

Microbiota2

Genomes3

Role in microbial2

Hosts5

Pathogen4

Neonatal sepsis1

Microbiology1

Infections4

Populations5

Transcriptional3

Specific microbial1

Proteins2

Organisms2

Metabolism3

Sepsis1

Salmonella2

Intracellular2

Communities5

Methods3

Sequences1

Species4

Sequence1

Protein2

Therapeutics1

Mechanisms3

Mucosal surfaces1

Enterococcus1

Candidate genes1

Functional1

Biofilm1

Characterization1

Inhibit2

Mutants1