• Protein body type one (PB-I) was isolated and purified from developing rice grain by a combination of sucrose density gradient centrifugation and treatment with pepsin. (elsevierpure.com)
  • The prerequisite was the generation of specific antisera directed against cyanelle SecY that allowed immunodetection of the protein on SDS gels from both membrane types separated by sucrose density gradient floatation centrifugation. (biomedcentral.com)
  • The density of the P9-specific membrane fraction was lower (approximately 1.13 g/cm 3 in sucrose) than the densities of the bacterial cytoplasmic and outer membrane fractions. (biomedcentral.com)
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of surface biotinylated proteins affinity-purified on lactoferrin-Sepharose showed biotinylated bands at Mr values of 22, 49, 55, 72, and 155 kDa. (bvsalud.org)
  • 2 ml peripheral blood were collected from the children with HSP and healthy controls and put into anticoagulant sodium heparin tube, PBMC was isolated by density gradient centrifugation. (biomedres.info)
  • Centrifugation: Types of rotors and centrifuges. (uc3m.es)
  • Some in vitro experiments have previously suggested that high-density lipoprotein (HDL) co-incubated with LDL prevents Cu 2+ -induced oxidation of LDL, while some other studies have observed an opposite effect. (biomedcentral.com)
  • DNA quality control is carried out by inner standard: making PCR reaction mixture, running the reaction, and separation of products by agarose gel electrophoresis. (elte.hu)
  • Determination of the composition of wheat strorage proteins by PCR markers: making the PCR reaction mixture for inner standard, running the reaction, and separation of products by agarose gel electrophoresis. (elte.hu)
  • By subjecting DNA origami samples to high centrifugal force in a density gradient media of glycerol, well-folded nanostructures and by-products are separated successfully, which are confirmed by agarose gel electrophoresis and atomic force microscopy (AFM). (chinaxiv.org)
  • Purified receptor preparations had specific activities of 0.5-3.5mumol a bungarotoxin binding sites per gram protein and sedi-mented as a single 125I-alpha bungarotoxin-binding species in sucrose density gradient centrifugation with S20,w =9.5. (bath.ac.uk)
  • In addition, sucrose density gradients, ultrafiltration ( 6 ), high performance liquid chromatography-based protocols ( 7 ) and immunoaffinity-capture methods ( 8 ), singly or combined with the application of UC, can provide high enrichment and purity of exosomes ( 9 ). (spandidos-publications.com)
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of surface biotinylated proteins affinity-purified on lactoferrin-Sepharose showed biotinylated bands at Mr values of 22, 49, 55, 72, and 155 kDa. (bvsalud.org)
  • Samples, divided into one MUC2 rich and one MUC5AC rich group, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and their amino acid contents were analysed. (hindawi.com)
  • Polyacrylamide gel electrophoresis under non-denaturing conditions of receptor-toxin complex showed a single protein component of similar mobility to that of acetylcholine receptor from Torpedo marmorata, whilst polyacrylamide gel electrophoresis of the purified receptor under denaturing conditions showed two major protein bands with molecular weights 42000 and 66000 respectively with the occasional appearance of minor components at 56000 and 85000. (bath.ac.uk)
  • Protein purity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry. (vittori-lab.com)
  • The principle of SV is very simple - the application of a high gravitational field to an initially uniform and well-mixed dilute solution of the protein sample, and the real-time imaging and mathematical analysis of the evolving concentration gradients arising from the centrifugal force. (nih.gov)
  • 6. One-step separation of endocytic organelles, Golgi/trans-Golgi network and plasma membrane by density gradient electrophoresis. (nih.gov)
  • 8. Separation of the plant Golgi apparatus and endoplasmic reticulum by free-flow electrophoresis. (nih.gov)
  • Gel electrophoresis -- The separation of the molecules of various ionic compounds dissolved in solution based on the compounds' different rates of movement through an electric field applied to the solution. (nih.gov)
  • We employ an effective and convenient purification approach to purify planar rectangle DNA origami structures through rate-zonal centrifugation. (chinaxiv.org)
  • Cesium-chloride gradient centrifugation -- A laboratory technique used to separate or purify nucleic acids in a solution. (nih.gov)
  • 15. Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free flow device (ZE-FFE). (nih.gov)
  • Knockdown of syntabulin expression with targeted small interfering RNA or interference with the syntabulin-kinesin-1 heavy chain interaction reduces mitochondrial density within axonal processes by impairing anterograde movement of mitochondria. (rupress.org)
  • 16. Free-flow electrophoresis: preparative applications to cell-free analyses of exocytotic membrane traffic. (nih.gov)