• Adenosine-based nucleotides, including adenosine tri-, di-, and mono-phosphate, are controlled through their rapid degradation by diphosphohydrolases and ecto-nucleotidases (NDAs). (humanbioscience.org)
  • To quantify these factors, we utilized reaction-diffusion numerical simulations of 1) adenosine triphosphate (ATP) hydrolysis into adenosine monophosphate (AMP) and 2) AMP into adenosine (Ado) via two representative nucleotidases, CD39 and CD73. (humanbioscience.org)
  • Membrane-bound 5'-nucleotidases display specificity toward adenosine monophosphates and are involved predominantly in the salvage of preformed nucleotides and in signal transduction cascades involving purinergic receptors. (wikipedia.org)
  • Ecto-5'-nucleotidase (NT5E, also known as CD73) hydrolyzes extracellular adenosine 5'-monophosphate (AMP) to adenosine in nociceptive circuits. (biomedcentral.com)
  • Ecto-5'-nucleotidase (NT5E) is a glycosyl phosphatidylinositol (GPI)-anchored membrane protein that catalyzes the hydrolysis of extracellular AMP to adenosine [ 1 ]. (biomedcentral.com)
  • ATP is sequentially degraded by the ecto-nucleotidases and 5'-nucleotidase enzymes to the weak adenosine agonist inosine as an end-product. (preprints.org)
  • Conversation The ecto-nucleoside triphosphate diphosphohydrolases (EC 3.6.1.5) symbolize a significant and ubiquitous category of ecto-nucleotidases. (bioinf.org)
  • Altogether, our studies suggest that CD39 and CD73 nucleotidase activity within junctional spaces can exploit their confinement and favorable electrostatic interactions to finely control nucleotide signaling. (humanbioscience.org)
  • Soluble 5'-nucleotidases are all known to belong to the haloacid dehalogenase superfamily of enzymes, which are two domain proteins characterised by a modified Rossman fold as the core and variable cap or hood. (wikipedia.org)
  • A nucleotidase is a hydrolytic enzyme that catalyzes the hydrolysis of a nucleotide into a nucleoside and a phosphate. (wikipedia.org)
  • In earlier studies, a number of compounds continues to be tested concerning their strength for inhibiting ecto-nucleotidases, frequently in intact cells or on cells with undefined enzyme varieties. (bioinf.org)
  • cN-I is a cytosolic nucleotidase(cN) characterized by its affinity toward AMP as its substrate. (wikipedia.org)
  • A nucleotidase is a hydrolytic enzyme that catalyzes the hydrolysis of a nucleotide into a nucleoside and a phosphate. (wikipedia.org)