acid sequence changes were observed in the 2 isolates from Samples were inoculated into Vero and A549 cell cultures patients who died. (cdc.gov)
The effect of curcumin on replication of influenza A virus (IAV) was examined in human lung cancer cell line A549, as well as in a mouse model. (turmericlife.com)
Outbreak2
All Brunei and Malaysia isolates from 2006 clustered major outbreak of EV71. (cdc.gov)
Their study of EV71 began in 1998, when a large EV71 outbreak occurred in Taiwan. (cgu.edu.tw)
Antiviral3
Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. (cdc.gov)
However, the lack of an efficient cell culture system hampers the development of novel antiviral strategies for HBV/HDV infections. (cdc.gov)
Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)
Infections1
A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. (cdc.gov)
Viability1
Specifically, schisandrathera D gradually decreased the levels of ANO1 protein and significantly reduced the cell viability in ANO1-expressing cells when compared to those in ANO1-knockout cells. (bvsalud.org)
Significantly1
Their participation contributed significantly to the laboratory diagnosis of EV71. (cgu.edu.tw)
Viral3
Viable CVA10 viruses were harvested by transfecting the viral mRNA into human rhabdomyosarcoma (RD) cells. (virosin.org)
Co-transfection of the viral replicon RNA and capsid expresser in human embryonic kidney 293T (HEK293T) cells led to the production of single round infectious particles (SRIPs). (virosin.org)
They subsequently focused on viral-host interactions, in which molecular targets for drug discovery were identified, and series of compounds were developed that inhibit EV71 replication. (cgu.edu.tw)
Virus1
Virus infection is initiated by the interaction between S protein and host cell surface receptors. (newvita.com)
Host cell1
The C-terminal domain (CTD) of S1 specifically binds to host cell receptors angiotensin-converting enzyme 2 (ACE2) or CD147, which causes the conformational change of S2. (newvita.com)
Human1
Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
Development1
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)
Enterovirus7
Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia. (nih.gov)
Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia and now is being recognized as an important neurotropic virus. (flutrackers.com)
In this paper the evidence of a virucidal effect of BR against human herpes simplex virus type 1 (HSV-1) and the enterovirus EV71 was provided. (frontiersin.org)
Enterovirus 71 (EV71) outbreaks occur periodically TGGGAGATAGGGTGGCAGATGTGATCGAGAGC-3 in the Asia-Pacifi c region. (cdc.gov)
Isolated EV71 strains gene fragments were sequenced, and phylogenetic relations formed a distinct lineage with low diversity within subgeno- of the sequences were examined using selected enterovirus group B5, suggesting recent introduction and rapid spread reference strains obtained from GenBank (Table 2). (cdc.gov)
Enterovirus 71 (EV71), a member of the family Picorna- ( 12 ). (cdc.gov)
Enterovirus 71 (EV71), a member of the family Picornaviridae and the genus Enterovirus , is a common cause of hand, foot, and mouth disease in children. (cdc.gov)
Neurologic complications2
In 2006, Brunei reported its first major outbreak of EV71 infections, associated with fatalities from neurologic complications. (cdc.gov)
EV71 was isolated from 34 patients (including 2 who died of severe neurologic complications), and an additional 7 isolates were obtained from Malaysian patients seen at the University Malaya Medical Center during the outbreak period in Brunei ( Table 1 ). (cdc.gov)
A randomized and open-label phase I clinical study registered with ClinicalTrials.gov #NCT01268787 , aims to evaluate the safety, reactogenicity and immunogenicity of a formalin-inactivated EV71 vaccine candidate (EV71vac) at 5- and 10-µg doses. (nih.gov)
Effective medications and prophylactic vaccine against EV71 infection are urgently needed. (flutrackers.com)
Based on the success of inactivated poliovirus vaccine, a prototype chemically inactivated EV71 vaccine candidate has been developed and currently in human phase 1 clinical trial. (flutrackers.com)
In this report, we present the development of a serum-free cell-based EV71 vaccine. (flutrackers.com)
Although the combination of chromatography and sucrose-gradient ultracentrifugation produced extremely pure EV71 infectious virus particles, the overall yield of vaccine was 7?10% as determined by a VP2-based quantitative ELISA. (flutrackers.com)
These results provide valuable information supporting the current cell-based serum-free EV71 vaccine candidate going into human Phase I clinical trials. (flutrackers.com)
Acute1
The expression of CD14 CD16 mononuclear cells in the CHD group was increased accordingly and correlated with acute HCMV infection. (bvsalud.org)
Antibody2
In this study we report the cross-neutralizing antibody responses from each volunteer against different subgenotypes of EV71 and CVA16. (nih.gov)
Thus, HCMV antibody as well as peripheral blood CD14 CD16 mononuclear cells can be used to monitor the occurrence and development of CHD. (bvsalud.org)
Responses1
These findings suggest that up-regulation of the HO/BVR axis is a useful mechanism for improving cells' responses to stress, and substances known to increase HO activity in vitro are being explored as potential drugs for the treatment of free radical-induced diseases ( Mancuso and Barone, 2009 ). (frontiersin.org)
Clinical1
This study aimed to evaluate whether circulating T cell subtypes are correlated with clinical response and dermatological toxicities in patients with advanced gastric and esophageal cancer receiving PD-1 inhibitor-based combination therapy (n = 203). (bvsalud.org)
Lines3
Bilirubin-IX-alpha, at concentrations 1-10 μM, close to those found in blood and tissues, significantly reduced HSV-1 and EV71 replication in Hep-2 and Vero cell lines, respectively. (frontiersin.org)
Moreover, 10 μM BR increased the formation of nitric oxide and the phosphorylation of c-Jun N-terminal kinase in Vero and Hep-2 cell lines, respectively, thus implying a role of these two pathways in the mechanism of antiviral activity of the bile pigment. (frontiersin.org)
Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
Mechanisms2
In eukaryotes, two distinct mechanisms have evolved in cells to initiate translation. (allindianpatents.com)
Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. (cdc.gov)
Culture1
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)