Ests expressed sequence tags. Medical search. Frequent questions

These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
In 2008, two human transcriptomes, composed of millions of transcript-derived sequences covering 16,000 genes, were published, and by 2015 transcriptomes had been published for hundreds of individuals. (wikipedia.org)
use microarray experiments to conclude that the human genome project will uncover many genes not previously discovered by EST sequencing (Nat Genet 2000, 26:315-318). (the-scientist.com)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
Targeted integration by homologous recombination enables in-situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatum . (umontreal.ca)
Sequences carrying the polymorphisms that distinguished wild and cultivated olives were analyzed and annotated, allowing to identify 124 candidate genes that have a functional role in flower development, stress response, or involvement in important metabolic pathways. (unboundmedicine.com)
One-third of the 471 genes predicted to encode transcription factors are differentially expressed during sexual development of S. commune . (nature.com)
Of these unique sequences, over 35% had significant similarities to known genes by BLASTX searches, which allowed the identification of 14,776 unique genes in the catfish. (auburn.edu)
The 2200 unigenes from the EST collection and the 62 immune-function targeted genes, together with other genes randomly selected without sequencing from the cDNA libraries, have been amplified and used to construct a cDNA microarray representing 3700 dolphin genes in duplicate for a total of 7400. (vin.com)
and he Began to use it to Identify genes welke are Expressed in the human brain. (multi-mania.be)
The NIH Office of Technology Transfer and Venter decided to use synthesis ESTs in an attempt to patent the genes they 'those given based on hun studies or mRNA expression in the human brain. (multi-mania.be)
10-5) to sequences in GenBank's non-redundant databases, indicating that a large proportion of novel genes are expressed in Heliconius male accessory glands. (ku.edu)
In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. (ku.edu)
Some laboratories have utilized transcriptomics to explore the response of chickpea to abiotic and biotic stresses such as drought, salinity, heat, cold, Fusarium oxysporum and Ascochyta rabiei diﬀerentially expressed genes and also established crosstalk between biotic and abiotic stress responses. (intechopen.com)
However, when applying this concept to genomes of multicellular organisms such as mammals, one has to deal with a large number of false positive predictions since many transcription factor genes are only expressed in specific tissues or cell types. (lu.se)
We developed TS-REX, a database/software system that supports the analysis of tissue and cell type-specific transcription factor-gene networks based on expressed sequence tag abundance of transcription factor-encoding genes in UniGene EST libraries. (lu.se)

Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)
Simple sequence repeats (SSRs) are highly variable features of all genomes. (biomedcentral.com)
There were those who argued that the objective of the program (sequencing genomes) was work for computers, not for researchers. (fapesp.br)
BACKGROUND: Long terminal repeat (LTR)-retrotransposons (LTR-RTs) are ubiquitous and make up the majority of nearly all sequenced plant genomes, whereas their pivotal roles in genome evolution, gene expression regulation as well as their epigenetic regulation are still not well understood, especially in a large number of closely related species. (bvsalud.org)

Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
Sequence based assays will be developed following the creation of cDNA libraries from selected tissues of A. senegal. (europa.eu)
Single pass sequencing of such libraries will be used to create a catalogue of Expressed Sequence Tags (ESTs) that will be used to develop single nucleotide polymorphic (SNP) markers. (europa.eu)
Random Expressed Sequence Tag (EST) clones have been isolated and sequenced from dolphin Peripheral Blood Leukocyte (PBL) cDNA libraries. (vin.com)
From the two cDNA-libraries 24,000 ESTs were collected and a total of 2200 unigenes have been sequenced and annotated ( www.marinegenomics.org ). (vin.com)

4 We screened the coding sequence of ESPN (GenBank accession number AL136880) by sequencing PCR amplified ESPN exons from genomic DNA of affected individuals in the two families. (bmj.com)
With previously existing ESTs in GenBank, this project brings the total of ESTs to nearly 500,000 in the catfish. (auburn.edu)
All ESTs have been deposited in GenBank. (auburn.edu)
dbEST is a large database of ESTs at GenBank , NCBI. (aporc.org)

Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond a few plastidial markers. (unboundmedicine.com)
During the nucleotide flow each of the beads is sequenced in parallel, with the polymerase extending the sequencing strand only if the nucleotide is complimentary to the template strand. (outsourcing-pharma.com)
If you sequence more than one nucleotide at once you get a stronger signal and we see a very linear response between the signal strength and the number of nucleotides added. (outsourcing-pharma.com)
Transcriptome analysis has facilitated the development of molecular markers such as simple sequence repeats (SSRs), single-nucleotide polymorphisms (SNPs) and potential intron polymorphisms (PIPs) that are being used to expedite the chickpea breeding programmes. (intechopen.com)

A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. (hawaii.edu)

Nuclear genome sequence and gene expression of an intracellular fungal endophyte stimulating the growth of cranberry plants. (umontreal.ca)
This thematic series of companion papers constitutes the initial exploration of the genome biology of the waterflea (Daphnia) building on the first complete crustacean genome sequence. (biomedcentral.com)
Application of such quality assessment measures, along with large resources of ESTs, should provide effective means for SNP identification in species where genome sequence resources are lacking. (auburn.edu)
2008. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita . (hutton.ac.uk)
We will be looking at working towards the $100,000 genome sequence landmark in 2007. (outsourcing-pharma.com)

The aim of this project was to generate clones and sequence data in the form of ESTs to provide a platform for the future molecular characterisation of the processes involved in protective dehydration. (nih.gov)
A total of 16,379 EST clones were generated and analysed using Blast and GO annotation. (nih.gov)
Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)

The first attempt at capturing a partial human transcriptome was published in 1991 and reported 609 mRNA sequences from the human brain. (wikipedia.org)
Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)

There were no hexameric repeats in EST of either species. (hawaii.edu)
To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius. (ku.edu)
Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)

This deep EST-SNP analysis provided important information on the genetic and genomic diversity of the olive complex, opening new opportunities to detect gene polymorphisms with potential functional and evolutionary roles, and to apply them in genomics-assisted breeding, highlighting the importance of olive germplasm conservation. (unboundmedicine.com)
The prediction of transcription factor binding sites in genomic sequences is in principle very useful to identify upstream regulatory factors. (lu.se)

Approximately one half of the ORFs are expressed in at least some of the ten tissues and cell types queried by the microarray. (the-scientist.com)
Over the last two decades, several laboratories have used a wide range of techniques encompassing expressed sequence tag (EST) analysis, serial analysis of gene expression (SAGE), microarray and next-generation sequencing (NGS) technologies for analysing the chickpea transcriptomes. (intechopen.com)

An initiative launched in 1997, exactly 10 years ago, it obtained its first international acknowledgement by means of the publication of an article on the cover of the journal Nature about the sequencing of the genetic code of the bacterium Xylella fastidiosa , which causes citrus variegated chlorosis, and attacks orange plantations (v. 406, n. 6.792, 13 July 2000). (fapesp.br)

The Sanger method of sequencing was predominant until the advent of high-throughput methods such as sequencing by synthesis (Solexa/Illumina). (wikipedia.org)
The technology is based on 'sequencing-by-synthesis' technology, which begins by breaking up the DNA sample into fragments that are between 300 and 500 bases in length. (outsourcing-pharma.com)

These fragments are then ligated by two short adaptors, which provide primers for both amplification and sequencing of the fragment as well as a biotin tag that immobilises it onto a streptavidin-coated bead. (outsourcing-pharma.com)
Its key features include a comprehensive microbial databases are increasingly comprehensive, it is unlikely sequence database for probe design and protocols for sam- that more than a fraction of the existing microbial ple preparation, amplification, labeling, hybridization, and sequence space has been explored. (cdc.gov)

In 1995, one of the earliest sequencing-based transcriptomic methods was developed, serial analysis of gene expression (SAGE), which worked by Sanger sequencing of concatenated random transcript fragments. (wikipedia.org)
However, these methods were largely overtaken by high throughput sequencing of entire transcripts, which provided additional information on transcript structure such as splice variants. (wikipedia.org)
The Pig Genome Oligo Set contains 10,665 70-mer probes representing (10,665) Sus Scrofa gene sequences with a hit to human, mouse, or pig gene transcript. (iastate.edu)

Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
In the nineteenth and twentieth centuries, the development of new microscopic and molecular techniques, including DNA sequencing, enabled scientists to confirm the hypothesis that chromosomes determine the sex of developing organisms. (asu.edu)

TS-REX covers ESTs from H. sapiens and M. musculus, and allows the characterization of both presence and specificity of transcription factors in user-specified tissues or cell types. (lu.se)

Consequently, the manner in which a transcriptome as a whole is expressed and regulated remained unknown until higher-throughput techniques were developed. (wikipedia.org)
The BioProject database is a searcheable collection of complete and incomplete (in-progress) large-scale molecular projects including genome sequencing and assembly, transcriptome, metagenomic, annotation, expression and mapping projects. (nih.gov)
This large scale EST sequencing project would allow the identification of majority of catfish transcriptome. (auburn.edu)
The transcriptome assemblies showed that 44,000-53,000 transcripts were expressed in the roots, leaves or flowers. (biomedcentral.com)

The short cDNA sequence Fragments when making this method are called expressed sequence tags , or ESTs. (multi-mania.be)

The use of GO annotation for analysis has facilitated the identification of a wide variety of ESTs associated with a number of different biochemical pathways involved in the dehydration and recovery process in O. arcticus. (nih.gov)
A variant of SAGE using high-throughput sequencing techniques, called digital gene expression analysis, was also briefly used. (wikipedia.org)
In: Expressed sequence tags - generation and analysis, Methods in Molecular Biology Vol. 533 (J. Parkinson, ed. (umontreal.ca)
The EST resource should also be valuable for identification of microsatellites, comparative genome analysis. (auburn.edu)
The parallel analysis of ESTs from the two closely related ictalurid catfishes should also provide powerful means for the evaluation of ancient and recent gene duplications, and for the development of high-density microarrays in catfish. (auburn.edu)
2007. Expressed sequence tag (EST) analysis of the pine wood nematode Bursaphelenchus xylophilus and B. mucronatus . (hutton.ac.uk)
Conclusion: This collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. (ku.edu)
In other types of sequence analysis, E has a similar meaning. (aporc.org)
In this study, we thoroughly examined the repetitive components of male and female H. scandens using next-generation sequencing data followed by bioinformatics analysis and florescence in situ hybridization (FISH). (bvsalud.org)
This study improves our understanding of the repetitive sequence organization of H. scandens genome and provides a basis for further analysis of their chromosome evolution process. (bvsalud.org)
Analysis of nasopharyngeal aspirates, blood, urine, and tis- tags rather than fluorescent dyes serve as reporters. (cdc.gov)

This significantly enhances the number of Collembolan ESTs in the public databases, providing useful comparative data within this phylum. (nih.gov)
Of these 9498 ORFs, 31% are completely novel and 29% are similar, but not identical, to sequences in public databases. (the-scientist.com)
Entrez is NCBI's primary text search and retrieval system that integrates the PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, structure, gene, genome, genetic variation and gene expression. (nih.gov)
EST databases and Web tools for EST projects. (umontreal.ca)
Maintain the EMBL database, one of the major public sequence databases. (aporc.org)

A guessed alignment of the sequences is first used to generate an expected scoring matrix representing the distribution of sequence characters in each column of the alignment, this pattern is matched to each sequence, and the scoring matrix values are then updated to maximize the alignment of the matrix to the sequences. (aporc.org)

Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)

When expressed in Xenopus laevis oocytes, hOAT3 mediated the transport of estrone sulfate ( K m = 3.1 μM), p -aminohippurate ( K m = 87.2 μM), methotrexate ( K m = 10.9 μM), and cimetidine ( K m = 57.4 μM) in a sodium-independent manner. (aspetjournals.org)
Xenopus laevis oocytes expressing hOAT1 supported probenecid-sensitive uptake of [ 3 H] p -aminohippurate ( K m = 4 μM), which was trans -stimulated in oocytes preloaded with glutarate. (aspetjournals.org)

With a good resource of ESTs, a large number of molecular markers can be identified, and issues related to alternative splicing and differential poly adenylation can be addressed at the genome-wide scale. (auburn.edu)

Researchers from several institutions and different disciplines, ranging from biology to medicine and including bioinformatics, which was virtually unknown at the time, worked together in a large virtual network that brought together as many as 60 laboratories to face a shared objective: the genetic sequencing of several organisms. (fapesp.br)

In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (nih.gov)
The deduced amino acid sequence of hOAT3 showed 36 to 51% identity to those of other members of the OAT family. (aspetjournals.org)

Expressed sequence tags (ESTs) have given researchers a quick if dirty look at the coding potential of the human genome. (the-scientist.com)
One of the things that is really important for high throughput DNA sequencing is for people to see that researchers can look at the whole genome of bacteria and viruses. (outsourcing-pharma.com)
The article, which described the first complete sequencing of DNA of a plant pathogen, was the work of a São Paulo network of 192 researchers supported by FAPESP. (fapesp.br)
Fernando identified the scientific opportunity, sequencing a genome, while Perez identified the ways and means - a virtual institute or network of researchers. (fapesp.br)

The ESTs are an enormous resource for SNP identification. (auburn.edu)
2009. Identification and functional characterisation of effectors in expressed sequence tags from various life cycle stages of the potato cyst nematode Globodera pallida . (hutton.ac.uk)
The system is capable of expressed sequence tag (EST) sequencing, the identification of small RNAs and transcription binding sites as well as whole genome sequencing and metagenomics. (outsourcing-pharma.com)

A TIGR sequence annotation tool has been developed at the Iowa State University. (iastate.edu)

Characterisation and mapping of expressed sequence tags or EST's to serve as anchored sequences tagged sites (STS). (le.ac.uk)

The importance of S. commune as a model system is also exemplified by the fact that its recombinant DNA constructs will express in other mushroom-forming fungi 5 . (nature.com)

There are two key contemporary techniques in the field: microarrays, which quantify a set of predetermined sequences, and RNA-Seq, which uses high-throughput sequencing to record all transcripts. (wikipedia.org)
In the 1980s, low-throughput sequencing using the Sanger method was used to sequence random transcripts, producing expressed sequence tags (ESTs). (wikipedia.org)

2018) using expressed sequence tag-short tandem repeats (EST-STRs) markers. (matis.is)

Concerted evolution refers to the pattern in which copies of multigene families show high intraspecific sequence homogeneity but high interspecific sequence diversity. (biomedcentral.com)

Anticipated orthologous ORFs in the regions above were defined as BLAT (Kent 2002) or SSearch (Pearson and Lipman 1988) sequence matches. (ubc.ca)

Over 300,000 putative SNPs have been identified, of which over 48,000 are high quality SNPs as defined by contig size of at least four sequences and the minor allele presence of at least twice in the contig. (auburn.edu)
The H. scandens genome has a high overall repetitive sequence composition, 68.30% in the female and 66.78% in the male genome, with abundant long terminal repeat (LTR) retrotransposons (RTs), including more Ty3/Gypsy than Ty1/Copia elements, particularly two Ty3/Gypsy lineages, Tekay and Retand. (bvsalud.org)

Transposable elements (TEs) and satellite DNAs, two major categories of repetitive sequences, are expected to accumulate in non-recombining genome regions, including sex-linked regions, and contribute to sex chromosome evolution. (bvsalud.org)

Expressed Sequence Tag (EST) sequencing is one of the most efficient means for gene discovery and gene expression profiling. (auburn.edu)

Results: We successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. (ku.edu)

They scan 350 Mb of finished and draft human sequence using three different gene-finding algorithms. (the-scientist.com)
Venter believed therein shotgun sequencing was the fastest and must effective way to get useful human genome data. (multi-mania.be)
[26] The goal of the company was to sequence the entire human genome and release it into tje public domain for non-commercial use in much less time and for much less cost dan the public human genome project. (multi-mania.be)
Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Beginning in the 1980s, many studies of human populations used the Y-chromosome gene sequences to trace paternal lineages. (asu.edu)

An algorithm for locating similar sequence patterns in a set of sequences. (aporc.org)

DNA polymersase is an enzyme used for all kinds of DNA techniques and sequencing. (timetoast.com)

This paper describes for the first time EST data from the arctic springtail (O. arcticus). (nih.gov)

A total of 600,000 sequencing attempts were made, generating a total of 438,321 quality ESTs. (auburn.edu)

Conserved Domains is a database of protein domains represented by sequence alignments and profiles for protein domains conserved in molecular evolution. (nih.gov)
In a database similarity search, the probability that an alignment score as good as the one found between a query sequence and a database sequence would be found in as many comparisons between random sequences as was done to find the matching sequence. (aporc.org)

The goal consequently put pressure on the public genome program and spurred verschillende groups to redouble hun policymaking to produce the full sequence. (multi-mania.be)

Anatomy10

Organisms23

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes50

Disciplines and Occupations3

Technology, Industry, Agriculture6

Information Science15

Health Care2

Genes18

Genomes4

Libraries5

GenBank4

Nucleotide4

Simple sequenc1

Genome sequence5

Clones4

MRNA2

Species5

Genomic2

Microarray2

20001

Synthesis2

Amplification2

Transcript3

Chromosomes2

Characterization1

Transcriptome4

CDNA sequence1

Analysis11

Databases5

Generate1

CDNAs1

Oocytes2

Molecular markers1

Genetic1

Amino2

Researchers4

Identification3

Annotation1

Characterisation1

Fungi1

Transcripts2

Markers1

Multigene1

20021

High2

Repetitive1

Gene discovery1

Results1

Human5

Patterns1

Techniques1

Data1

Total1

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Public1