• surface plasmon r
  • The binding ability of bevacizumab to murine VEGF-A was analyzed by Western blot, ELISA, and surface plasmon resonance. (arvojournals.org)
  • Western blot analysis, ELISA, and the surface plasmon resonance assay showed that bevacizumab binds murine VEGF-A. (arvojournals.org)
  • Combining in silico CDR-grafting, structure-based back mutation, and, supported by our patented FASEBA (Fast Screening for Expression, Biophysical-properties and Affinity) screening, Biacore Surface Plasmon Resonance platform and functional assay platform, the antibody humanization service is offered at GenScript to generate humanized antibody maintaining the affinity and specificity of parental antibody and optimized activity , productivity and thermostability . (genscript.com)
  • binding assays such as using ELISA , Biacore Surface Plasmon Resonance and a suite of biological assays available to support the characterization and selection. (genscript.com)
  • nucleic acids
  • Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. (wikipedia.org)
  • A hybridization assay comprises any form of quantifiable hybridization,[clarification needed] i.e. the quantitative annealing[clarification needed] of two complementary strands of nucleic acids, known as nucleic acid hybridization. (wikipedia.org)
  • vitro
  • Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! (mybiosource.com)
  • Wi, Inc. (pronounced as an initialism, "double-u i.") is an American medical device development corporation incorporated and headquartered in Englewood, CO. Wi specializes in in-vitro diagnostic microfluidic disposable cartridges and instruments, biological assays on and off disposable cartridges, and electromechanical devices. (wikipedia.org)
  • specificity
  • The advent of somatic cell fusion techniques ( 2 ) permitted the production of mAbs with high sensitivity and specificity to digoxin (Dig) 4 ( 3 ) for use in the study of Ab structure-function relationships, development of clinical assays, and emergency treatment for life-threatening digitalis overdoses ( 4 , 5 ). (jimmunol.org)
  • The specificity of the hybridization-ligation assay for ligation at the 3'-end is particularly relevant because the predominant nucleases in blood are 3' to 5' exonucleases. (wikipedia.org)
  • The dual ligation hybridization assay (DLA) extends the specificity of the hybridization-ligation assay to a specific method for the parent compound. (wikipedia.org)
  • Despite hybridization-ligation assay's robustness, sensitivity and added specificity for the 3'-end of the oligonculeotide analyte, the hybridization-ligation assay is not specific for the 5' end of the analyte. (wikipedia.org)
  • detects
  • The assay detects a molecule, often in low concentrations, that is a marker of disease or risk in a sample taken from a patient. (wikipedia.org)
  • Designed by Luminex partner, GenArraytion, Inc. , the xMAP ® MultiFLEX™ Zika RNA Assay detects Zika viral RNA in serum, plasma, or urine (collected alongside a patient-matched serum or plasma specimen). (corporate-ir.net)
  • As for the hybridization-ligation assay, the DLA is specific for the parent compound because the efficiency of ligation over a bulge loop is low, and thus the DLA detects the full-length analyte with both intact 5' and 3'-ends. (wikipedia.org)
  • Utilize
  • Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
  • concentration
  • Generally, in the case of nucleic acid hybridization, monovalent salt concentration and temperature are controlled for hybridization and wash stringency, contrary to a traditional ELISA, where the salt concentration will usually be fixed for the binding and wash steps (i.e. (wikipedia.org)
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
  • clinical
  • Subsequent human "biomarker" studies suggest that inter-individual clinical (and biological) heterogeneity in humans often underlies treatment resistance in pain and/or depression. (uth.edu)
  • The xMAP ® MultiFLEX™ Zika RNA Assay is available for purchase by laboratories that are certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) to perform high complexity tests. (corporate-ir.net)
  • proteins
  • This guide outlines steps to perform lauryl maltoside extractions of functional enzymes and proteins for use with our ELISA and dipstick activity assays. (abcam.com)
  • Many of our assays are reactive with rodent proteins and were developed with rodent tissues (mouse and rat). (abcam.com)
  • Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites, and biological molecules in unnatural locations or concentrations) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological systems. (wikipedia.org)
  • diagnostic
  • As a leader in multiplex diagnostic testing for infectious diseases, we are proud to help enable healthcare providers to rapidly detect Zika virus with this assay today, and we intend to expand our coverage to include a broader array of mosquito-borne disease agents in the future to aid in treatment decisions for potentially afflicted patients. (corporate-ir.net)
  • Abcam
  • Today I would like to introduce a brand new technology from Abcam called the SimpleStep ELISA. (abcam.com)
  • analyte
  • The company is transforming global healthcare and life-science research through the development, manufacturing and marketing of proprietary instruments and assays utilizing xMAP ® open-architecture multi-analyte platform, MultiCode ® real-time polymerase chain reaction (PCR), and multiplex PCR-based technologies, that deliver cost-effective rapid results to clinicians and researchers. (corporate-ir.net)
  • Like other hybridization assays, it relies on complementarity, where the capture probe competes between the analyte and the tracer-a labelled oligonucleotide analog to the analyte. (wikipedia.org)
  • microtiter
  • The SimpleStep ELISA begins with removing the number of microtiter strips needed to setup your assay. (abcam.com)
  • Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. (wikipedia.org)
  • Sample reactions can be assayed in 6-1536 well format microtiter plates. (wikipedia.org)
  • reactions
  • Using multiple lasers or LEDs and high-speed digital-signal processors, the analyzer reads multiplex assay results by reporting the reactions occurring on each individual microsphere. (corporate-ir.net)
  • accurately
  • Modern drugs are more potent, which has required more sensitive bioanalytical assays to accurately and reliably determine these drugs at lower concentrations. (wikipedia.org)
  • targets
  • The multi-target assay uses the Luminex® 100/200™ or MAGPIX® instrument, or other authorized instruments, to simultaneously test for six genetic targets of the Zika virus. (corporate-ir.net)
  • beads
  • Systems using xMAP Technology perform discrete assays on the surface of color coded beads known as microspheres, which are then read in a compact analyzer. (corporate-ir.net)
  • Hybridization assays can be in solution or on a solid support such as 96-well plates or labelled beads. (wikipedia.org)
  • capabilities
  • Luminex's xMAP ® Technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. (corporate-ir.net)
  • specific
  • 2008). CPsV-EG was detected on the basis of biological indexing by graft inoculation which gave oak leaf pattern (OLP) on Dweet tangor and serological assay by DAS-ELISA using Mab specific CPsV. (wikipedia.org)
  • The 1930s also saw the rise of pharmacokinetics, and as such the desire for more specific assays. (wikipedia.org)
  • Instrument companies have designed plate readers which can detect specific biological, chemical or physical events in samples stored in these plates. (wikipedia.org)
  • samples
  • It is cheap, fast, and reliable, but it has limitations when used for biological samples. (sigmaaldrich.com)
  • Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. (wikipedia.org)
  • limitations
  • The limitations with the hybridization-ligation assay also apply to the dual ligation assay, with the 5'-end in addition to the 3'-end requiring to have a free hydroxyl (or a phosphate group). (wikipedia.org)
  • strips
  • ELISA plates may now be assembled from twelve separate strips of eight wells, making it easier to only partially use a plate. (wikipedia.org)
  • bound
  • Some newer types are called "mix-and-measure" assays because they do not require separation of bound ligands. (wikipedia.org)
  • format
  • If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. (mybiosource.com)
  • sensitive
  • It is used mainly in relation to hematological malignancies but the developments of more sensitive assays have stimulated investigations for its use in relation to solid tumors. (wikipedia.org)
  • development
  • Through the development of RIA technology, researchers have been able to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. (wikipedia.org)
  • Test
  • Wi has worked on many point-of-care tests, including designing and prototyping a disposable card, hardware, and mixing on-card assay, and readying the Point-of-Care DNA-MRSA test for full PC control and lab use. (wikipedia.org)
  • For a CD4 Point-of-Care test, Wi designed and pilot-built the card, did design assay and integration, and worked with the imaging software and hardware interface. (wikipedia.org)
  • Home pregnancy test involves ELISA to detect the increase of human chorionic gonadotropin (hCG) during pregnancy. (wikipedia.org)