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Peptide Initiation FactorsEukaryotic Initiation Factor-2Eukaryotic Initiation Factor-4EEukaryotic Initiation FactorsEukaryotic Initiation Factor-4GEukaryotic Initiation Factor-4FEukaryotic Initiation Factor-3Eukaryotic Initiation Factor-2BProtein BiosynthesisEukaryotic Initiation Factor-4APeptide Chain Initiation, TranslationaleIF-2 KinaseReticulocytesEukaryotic Initiation Factor-1RibosomesEukaryotic Initiation Factor-5PhosphorylationRNA CapsRNA, Transfer, MetEukaryotic CellsMolecular Sequence DataRNA Cap AnalogsRNA, MessengerTOR Serine-Threonine KinasesProtein KinasesProkaryotic Initiation Factor-2Poly(A)-Binding ProteinsRNA-Binding ProteinsRibosomal Protein S6 KinasesAmino Acid SequencePolyribosomesRabbitsPhosphoproteinsGuanosine TriphosphateProtein BindingBase SequenceCodon, InitiatorCarrier ProteinsRibosomal Protein S6RNA Cap-Binding ProteinsProteinsActivating Transcription Factor 4Saccharomyces cerevisiaeRibosome Subunits, Small, EukaryoticProkaryotic Initiation Factor-3Guanosine DiphosphateRibosomal Protein S6 Kinases, 70-kDaSirolimusHeLa CellsRNA, Transfer, Amino AcylProtein-Serine-Threonine KinasesBinding Sites5' Untranslated RegionsRNA, Double-StrandedCell-Free SystemKineticsCell LineMutationGuanine Nucleotide Exchange FactorsSignal TransductionSaccharomyces cerevisiae ProteinsMacromolecular SubstancesProtein Structure, TertiaryHeminSequence Homology, Amino AcidRNA, TransferProtein Synthesis InhibitorsEndoplasmic ReticulumNucleic Acid ConformationProtein SubunitsHemeRibosome Subunits, Large, EukaryoticPotyvirusRibosomal ProteinsTranscription Factor CHOPTranscription, GeneticAdaptor Proteins, Signal TransducingNuclear Cap-Binding Protein ComplexCloning, MolecularEdeineSequence AlignmentEscherichia coliRecombinant ProteinsLysineProkaryotic Initiation FactorsLeucineRNA, ViralTriticumMolecular WeightMethionineMultiprotein ComplexesGene Expression RegulationAmino AcidsPicornaviridaeEnzyme ActivationBlotting, WesternSerineGuanine NucleotidesPeptide Elongation Factor 2Models, Molecular
MRNAMRNAsHelicaseEIF4EMammalianPhosphorylationTranslationalArabidopsisProtein synthesisHomologous
MRNA3
  • eIF4A is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of mRNA translation. (aber.ac.uk)
  • Oryza sativa Japonica Group eukaryotic initiation factor 4A-1-like (LOC4341966), transcript variant X1, mRNA. (genscript.com)
  • Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. (affbiotech.com)
MRNAs6
  • RNA-immunoprecipitation sequencing (RIP-Seq) of the two eIF4A paralogs revealed that eIF4A1 preferentially binds to mRNAs containing terminal oligopyrimidine (TOP) motifs, whose translation is rapidly repressed upon mTOR inhibition. (biorxiv.org)
  • Ribosome profiling revealed that the deletion of EIF4A1 , but not EIF4A2 , rendered the translation of TOP mRNAs resistant to mTOR inactivation. (biorxiv.org)
  • Moreover, eIF4A1 enhances the affinity between TOP mRNAs and LARP1 and thus ensures stronger translation repression upon mTORC1 inhibition. (biorxiv.org)
  • The mechanisms involved in the stress-induced translation have been investigated for a small number of key transcription factors (for example, yeast general control nondepressible 4 (GCN4) 12 and mammalian activating transcription factor 4 (ATF4) 13 ), whose translation is normally inhibited by the uORFs in the 5' leader sequences of their mRNAs. (biorxiv.org)
  • Moreover, recent global ribosome-sequencing (Ribo-seq, sequencing of ribosome-protected RNA fragments) studies have shown that uORFs are a prevalent feature in eukaryotic mRNAs, not limited to these few well-studied examples 19 - 21 . (biorxiv.org)
  • eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. (affbiotech.com)
Helicase1
  • In vitro, wild type recombinant eIF4A1 and its phospho-null variant both support translation in cell free wheat germ extracts dependent upon eIF4A, but the phosphomimetic variant does not support translation and was also deficient in ATP hydrolysis and helicase activity. (aber.ac.uk)
EIF4E1
  • Eukaryotic translation initiation factor 4 gamma has been shown to interact with MKNK1, EIF4A1, EIF4E, MKNK2 and PABPC1. (wikipedia.org)
Mammalian2
  • Two mammalian eIF4A paralogs, eIF4A1 and eIF4A2, have been assumed to be redundant because of their high homology, and the difference in their functions has been poorly understood. (biorxiv.org)
  • Previously, we found that eukaryotic initiation factor (eIF) 4A interacts with cyclin dependent kinase A (CDKA), the plant ortholog of mammalian CDK1. (aber.ac.uk)
Phosphorylation1
  • Upon stress, phosphorylation of eukaryotic Initiation Factor 2α (eIF2α) decreases the available ternary complex, resulting in reduced translation initiation from the start codons of uORFs (uAUGs) and prolonged scanning of the preinitiation complex to translate the downstream main open reading frames (mORFs) to promote cell survival 12 - 15 . (biorxiv.org)
Translational1
  • Here, we show that eIF4A1, but not eIF4A2, enhances translational repression during the inhibition of mechanistic target of rapamycin complex 1 (mTORC1), an essential kinase complex controlling cell proliferation. (biorxiv.org)
Arabidopsis1
  • In vivo, a phospho-null (APGR) variant of the Arabidopsis eIF4A1 protein retains the ability to functionally complement a mutant (eif4a1) plant line lacking eIF4A1, whereas a phosphomimetic (EPGR) variant fails to complement. (aber.ac.uk)
Protein synthesis1
  • Our data show that the distinct protein interactions of these highly homologous translation factor paralogs shape protein synthesis during mTORC1 inhibition and provide a unique example of the repressive role of a universal translation activator. (biorxiv.org)
Homologous1