• The initiator element (Inr) is the most common sequence found at the transcription start site of eukaryotic genes. (wikipedia.org)
  • Though the Inr element is not fully understood it has been recognized as the most frequently occurring sequence at the start site of genes in multiple species. (wikipedia.org)
  • Drosophila possesses two FGF receptors that are encoded by the heartless and breathless genes. (sdbonline.org)
  • article{gross_drosophila_1996, title = {Drosophila immunity: a comparative analysis of the Rel proteins dorsal and Dif in the induction of the genes encoding diptericin and cecropin}, author = {I Gross and Philippe Georgel and Christine Kappler and Jean-Marc Reichhart and Jules A Hoffmann}, issn = {0305-1048}, year = {1996}, date = {1996-04-01}, journal = {Nucleic Acids Res. (cnrs.fr)
  • volume = {24}, number = {7}, pages = {1238--1245}, abstract = {In Drosophila, bacterial challenge induces the rapid transcription of several genes encoding potent antibacterial peptides. (cnrs.fr)
  • The upstream sequences of the diptericin and cecropin Al genes, which have been investigated in detail, contain two, respectively one sequence element homologous to the binding site of the mammalian nuclear factor kappaB. (cnrs.fr)
  • Both the amino acid sequences of the homeodomains derived from these genes and the order of the genes within each cluster have been conserved during evolution (1, 2). (nih.gov)
  • Some of the Drosophila homeobox genes in the Antp and Ubx clusters of genes (4) function as homeotic selector genes (5), which determine unique parts of the body. (nih.gov)
  • DNA sequence comparisons of genes encoding Toll-related proteins in Drosophila , reptiles, birds and in mammals have revealed that the genes are well conserved and have evolved independently from a common ancestor gene [ 8 ]. (biomedcentral.com)
  • ALIGN - DS6900.UUE Alignment of pseudoeta-globin genes and surrounding sequences from primates and goat. (bio.net)
  • NUCLEAR_REC_DBD_2} {BEGIN} ********************************************************************** * Nuclear hormone receptors DNA-binding domain signature and profile * ********************************************************************** Nuclear hormone receptors are ligand-activated transcription factors that regulate gene expression by interacting with specific DNA sequences upstream of their target genes. (expasy.org)
  • Beginning with his invention and development of EST sequencing to identify sequences of large numbers of expressed genes, Dr. Venter went on to determine the structural sequence of the hereditary materials of other mammalian, insect, bacterial, and viral genomes. (cornell.edu)
  • Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (lookformedical.com)
  • Transient over-expression of runt under the control of a Drosophila heat-shock promoter caused stripe-specific defects in the expression patterns of the pair-rule genes hairy and even-skipped but had a more uniform effect on the secondary pair-rule gene fushi tarazu . (biologists.com)
  • A DNA sequence initially defined as a consensus binding site for the Engrailed protein is also recognized by several other homeodomain proteins and mediates the transcriptional action of these regulators in transfected tissue culture cells. (nih.gov)
  • Functional studies have shown that these kappaB-related elements can be the target for the Drosophila Rel proteins dorsal and Dif. (cnrs.fr)
  • In addition, origin activity can be regulated from sequences such as "locus control regions" that are many kilobases distal to the OBR but affect the accessibility of initiation sites to replication proteins. (nih.gov)
  • First identified on the basis of sequence similarity with the Drosophila protein Toll, TLRs are members of an ancient superfamily of proteins, which includes related proteins in invertebrates and plants. (biomedcentral.com)
  • The deduced amino acid sequence of p270 reported here indicates that it is a member of an evolutionarily conserved family of proteins distinguished by the presence of a DNA binding motif termed ARID (AT-rich interactive domain). (cshl.edu)
  • The approximately 100-residue ARID sequence is present in a series of proteins strongly implicated in the regulation of cell growth, development, and tissue specific gene expression. (cshl.edu)
  • In contrast, p270 shows no sequence preference in its DNA binding activity, thereby demonstrating that AT-rich binding is not an intrinsic property of ARID domains and that ARID family proteins may be involved in a wider range of DNA interactions. (cshl.edu)
  • Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments. (lookformedical.com)
  • Based on their amino acid sequences and antibacterial activities, most of the peptides are divided into five groups including cecropin, insect-defensins, glycine-rich proteins, proline-rich proteins, and lysozymes. (hindawi.com)
  • Although SS consensus sequence and splicing regulatory proteins are known to influence SS usage, the mechanisms ensuring the effective suppression of cryptic SS are insufficiently explored. (uni-koeln.de)
  • In a second laboratory, stumps was discovered in a search for mutations affecting morphogenetic movements in the Drosophila embryo (Vincent, 1998). (sdbonline.org)
  • Drosophila protein knirps (kni), a zygotic gap protein required for abdominal segmentation of the Drosophila embryo. (expasy.org)
  • Finally, genetic experiments demonstrated that regulation of gap gene expression by runt is a normal component of the regulatory program that generates the segmented body pattern of the Drosophila embryo. (biologists.com)
  • CRISPR-Cas9-based genome editing has transformed the life sciences, enabling virtually unlimited genetic manipulation of genomes: The RNA-guided Cas9 endonuclease cuts DNA at a specific target sequence and the resulting double-strand breaks are mended by one of the intrinsic cellular repair pathways. (life-science-alliance.org)
  • We quantified TLs in whole flies and dissected brains and validated the feasibility of our approach in detecting new TE insertions in aging Drosophila genomes when small RNA and RNA interference (RNAi) pathways are compromised. (nih.gov)
  • PCR Closing gaps in the assembly PCR with primers targeting particular areas on the two JP environmental genomes have been employed to confirm the genome assembly, raise sequencing coverage and reconfirm the presence of notable genome characteristics. (tcr-signal.com)
  • Here we resequence the mitochondrial genomes of 169 Drosophila Genetic Reference Panel lines, identifying 231 variants that stratify along 12 mitochondrial haplotypes. (nature.com)
  • The conservation of different family members varied widely across the 12 sequenced Drosophilid genomes. (cnrs.fr)
  • A mouse homologue of the Drosophila tumor suppressor l(2)tid gene defines a novel Ras GTPase-activating protein (RasGAP)-binding protein. (embl.de)
  • Here we report the cloning and characterization of a novel GAP-binding protein, mTid-1, a DnaJ chaperone protein that represents the murine homolog of the Drosophila tumor suppressor l(2)tid gene. (embl.de)
  • In a study of 1800+ distinct human promoter sequences it was found that 49% contain the Inr element while 21.8% contain the TATA box. (wikipedia.org)
  • We suggest that this specific pattern of expression results from combined action at sequences within the hsp70 promoter fragment and the homeodomain binding sites. (nih.gov)
  • However, the chromatin-remodeling complexes generally do not have DNA sequence specificity, and it is therefore not known whether these complexes are targeted to specific sites such as the promoter region. (nih.gov)
  • Drosophila protein ultraspiracle (usp) (or chorion factor 1), which binds to the promoter region of s15 chorion gene. (expasy.org)
  • We find that inhibition of splicing primarily increases editing levels at hundreds of sites, suggesting that reduced splicing efficiency extends the exposure of intronic and exonic sequences to ADAR enzymes. (omicsdi.org)
  • Thus, the EJC protects the transcriptome of mammalian cells from inadvertent loss of exonic sequences and safeguards the expression of intact, full-length mRNAs. (uni-koeln.de)
  • Viral origins contain a specific core DNA sequence that is required for initiation to proceed efficiently, whereas the requirement for specific DNA sequences is greatly diminished in single cell eukaryotes and virtually absent in multicellular eukaryotes. (nih.gov)
  • Finally, metazoan ORCs do not require binding to specific DNA sequences in order to initiate assembly of a pre-RC. (nih.gov)
  • Early embryos undergoing rapid cell cleavage (e.g. frogs, flies, sea urchin, and fish) initiate DNA replication with no obvious requirement for any specific DNA sequences, and Drosophila ORC or human ORC can replace Xenopus ORC in frog egg extracts with the same result. (nih.gov)
  • We have initiated a genetic analysis of the Drosophila homolog of the Myc oncoprotein (dMyc),which is encoded by the dm locus. (silverchair.com)
  • COUP transcription factor (also known as ear-3), and its Drosophila homolog seven-up (svp). (expasy.org)
  • The Drosophila Runt protein is a member of a new family of transcriptional regulators that have important roles in processes extending from pattern formation in insect embryos to leukemogenesis in humans. (biologists.com)
  • Sequencing the human genome. (trashworldnews.com)
  • J. Craig Venter, noted geneticist and A.D. White Professor-at-Large, is the founder of Celera Genomics, whose team of researchers was one of two which first completed sequencing of the human genome. (cornell.edu)
  • Mutations have been described in the Drosophila heartless gene that eliminate development of the single somatic muscle and the subset of pericardial cells that express Even-skipped (Eve) in the dorsal region of the embryonic mesoderm. (sdbonline.org)
  • Drosophila protein embryonic gonad (egon). (expasy.org)
  • To address this question, we quantified TE Landscapes (TLs) via whole genome sequencing of young and aged Drosophila strains of wild-type and mutant backgrounds. (nih.gov)
  • All other numbering was relative to the full-length open reading frame of A/goose/Guangdong/1/1996 protein sequences. (cdc.gov)
  • In this genome-editing system, the Cas9 enzyme forms a complex with a guide RNA (gRNA), which provides targeting specificity through direct interaction with homologous 18-22 nt target sequences in the genome. (vectorbuilder.cn)
  • There is one file for each PDB protein containing all protein sequences deemed homologous to this protein, in the form of multiple sequence alignments. (bio.net)
  • Sp1 is then able to regulate the activation and initiation of transcription The Inr element sequence is located -6 bp upstream of the transcription start site and continues to around +45 bp downstream. (wikipedia.org)
  • In contrast, NHEJ ligates the two broken ends of the DNA without a donor template, often resulting in random insertions or deletions (indels) that can disrupt coding sequences at the target site (for review see reference 14 ). (life-science-alliance.org)
  • PCR assays were used to identify somatic R2 insertions in isolated adult tissues and larval imaginal discs of Drosophila simulans . (biomedcentral.com)
  • The somatic insertions had 5' junction sequences characteristic of germline insertions suggesting they represented authentic retrotransposition events. (biomedcentral.com)
  • Sequences known to belong to this class detected by the profile: ALL. (expasy.org)
  • ESEV, H5N1 PDZ-binding motif amino acid consensus sequence. (cdc.gov)
  • GalNAc-Ts do not recognize a consensus sequence or motif, and each isoenzyme shows unique specificity towards acceptor sites on a substrate. (nih.gov)
  • Cas9 screens the genome and cleaves within sequences complementary to the gRNA, provided they are immediately followed by the protospacer adjacent motif (PAM) NGG. (vectorbuilder.cn)
  • We derive a consensus hinge motif, for Drosophilid inhibitory serpins, which differs somewhat from that of the vertebrate consensus. (cnrs.fr)
  • This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. (nih.gov)
  • PubMed=2644044 [ 5] Segraves W.A. "Something old, some things new: the steroid receptor superfamily in Drosophila. (expasy.org)
  • Origins in multicellular organisms generally lack ARS activity, although they do exhibit "replicator" activity (the ability to impart origin activity when translocated to other chromosomal sites), and they can be inactivated by sequence alterations. (nih.gov)
  • These include computational strategies such as Sorting Intolerant From Tolerant (SIFT) [ 84 ] and PolyPhen, [ 85 , 206 ] which use conservation of sequencing among different organisms or related sequences. (medscape.com)
  • These scattered errors can be tolerated in some applications but become extremely problematic when "deep sequencing" genetically heterogeneous mixtures, such as tumors or mixed microbial populations. (justia.com)
  • Origins in the fission yeast Schizosaccharomyces pombe lack a genetically required consensus sequence, although they do exhibit "autonomously replicating sequence" (ARS) activity. (nih.gov)
  • Genome editing is achieved by precisely targeting the nuclease activity of a modified bacterial protein (Cas9) via a user-defined guide RNA to a specific DNA sequence ( 1 ). (life-science-alliance.org)
  • The bacteriophage φC31 encodes an integrase that mediates efficient, sequence-specific recombination between phage attachment sites (called attP) and bacterial attachment sites (called attB). (vectorbuilder.cn)
  • The GYPSY13-I_AG consensus was reconstructed after multiple alignment of 11 copies. (girinst.org)
  • 3.4, Apr 91) Alu sequence database and alignment ALU Software: Software for MS-DOS computers DOS_SOFTWARE Software for Apple Macintosh MAC_SOFTWARE Software for UNIX UNIX_SOFTWARE Software for VAX/VMS VAX_SOFTWARE Other software, GenBank Clearinghouse, etc. (bio.net)
  • While 16% contained only one mismatch TFIID and subunits are very sensitive to the Inr sequence and nucleotide changes have been shown to drastically change the binding affinity. (wikipedia.org)
  • Summary of Directories of the EMBL File Server ------------------------------------------- Databases (* = new or updated): * EMBL Nucleotide Sequence Database NUC (Rel. (bio.net)
  • 17, Jun 91) REFLIST * Functional Analysis of Nucleotide Sequences bibliography REFLIST (FANS_REF Rel. (bio.net)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • It is also called nucleotide sequence. (lookformedical.com)
  • Here, we show that in Drosophila , the detected Cas9-mediated editing events frequently resulted in germline-transmitted exchange of chromosome arms-often without indels. (life-science-alliance.org)
  • Our Drosophila Cas9 expression pUASTB vector system has the capability to utilize either the Drosophila P-element transposon system (like pUAST) or the bacteriophage φC31 integration system (like pUASTattB) for Cas9 insertion into the genome. (vectorbuilder.cn)
  • Utilizing the CRISPR/Cas9 system in Drosophila allows the rapid generation of knockout lines by simply delivering either an all-in-one vector (a single vector expressing both Cas9 and gRNA) or separate vectors for driving Cas9 and gRNA expression, respectively. (vectorbuilder.cn)
  • Although, in theory, DNA subpopulations of any size should be detectable when deep sequencing a sufficient number of molecules, a practical limit of detection is imposed by errors introduced during sample preparation and sequencing. (justia.com)
  • Initially, the PCI domain was defined as the region of detectable sequence similarity between the components mentioned above. (biomedcentral.com)
  • Structural features of human members of the TLR protein family and the archetypal Drosophila Toll protein. (biomedcentral.com)
  • The ARID consensus and other structural features are common to both p270 and yeast SWI1, suggesting that p270 is a human counterpart of SWI1. (cshl.edu)
  • While exome sequencing may have limited resolution for certain changes such as CNVs or structural variation, and coverage may be variable based on enrichment methods used, it is still useful for both dominant Mendelian disorders and for de novo (sporadic) mutations for sporadic Mendelian diseases. (medscape.com)
  • Several of these ADARB1-mediated editing events change splice consensus sequences, therefore strongly influencing splicing of some mRNAs. (omicsdi.org)
  • ORDERFORM.TXT EMBL Data Library subscription form 1991/2 - ORDERINFO.TXT EMBL Data Library subscription information for 1991/2 - CDROM_INDEX.HQX Description of binary indices provided on current EMBL CD-ROMs for access to sequence database files. (bio.net)
  • 9106, Jun 91) REBASE * tRNA sequence and gene sequence database (1991) TRNA * TFD, Transcription Factor Database (Ver 3.0, Jun 91) TFD * The E.coli Database (Rel. (bio.net)
  • Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. (biomedcentral.com)
  • The advent of massively parallel DNA sequencing has ushered in a new era of genomic exploration by making simultaneous genotyping of hundreds of billions of base-pairs possible at small fraction of the time and cost of traditional Sanger methods [1]. (justia.com)
  • [ 87 , 88 ] Ultimately, confirmation of the candidate gene identified by exome sequencing as the disease gene requires not just confirmation via Sanger sequencing, but functional characterization as well. (medscape.com)
  • The sequence similarity between the intracellular domains of the TLRs and the mammalian interleukin-1 and interleukin-18 cytokine receptors reflects the use of a common intracellular signal-transduction cascade triggered by these receptor classes. (biomedcentral.com)
  • stumps has been cloned and although it appears to be a novel protein, it possesses several sequences characteristic of a signal transduction protein (Vincent, 1998). (sdbonline.org)
  • Studies have shown that promoters with a functional Inr are more likely to lack a TATA box or to possess a degenerate TATA sequence. (wikipedia.org)
  • This sequence encompasses where the RNA polymerase will begin transcribing. (wikipedia.org)
  • The zinc finger core binds in the major groove and recognizes the first three GAG bases of the consensus in a manner similar to that seen in other classical zinc finger-DNA complexes. (nih.gov)
  • Because the nuclease domain does not have sequence specificity of its own, the nuclease domain fused to the transcription factor cleaves DNA near where the transcription factor portion of the fusion protein binds. (nih.gov)
  • EKLF binds to a specific DNA sequence, the CACCC box, in vitro, and it has therefore been assumed that the EKLF-CACCC box interaction controls EKLF recruitment in vivo. (nih.gov)
  • It is related in sequence and structure to ACTIN and binds ATP. (nih.gov)
  • HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
  • It binds as a dimer with each monomer recognizing a six base pair sequence of DNA. (expasy.org)
  • D. su zukii, unlike most other Drosophila species, is an agricultural pest that lays its eggs in soft-skinned, unspoiled fruits like cherries, grapes, and berries (Bolda et al. (researchgate.net)
  • Body segments are specified early in Drosophila development, thus the detection of the same somatic insertion in cells from multiple tissues suggested that the R2 retrotransposition events had occurred before the blastoderm stage of Drosophila development. (biomedcentral.com)
  • Other sequence(s) detected in Swiss-Prot: NONE. (expasy.org)
  • However, studies in Drosophila simulans indicate that in individuals where R2-inserted units are distributed throughout the rDNA locus, inserted rDNA units are transcribed [ 11 - 13 ]. (biomedcentral.com)
  • The critical Reactive Centre Loop (RCL) sequence, including the target proteinase cleavage site, is strongly conserved in inhibitory serpins, although there are 3 exceptional sets of orthologues in which the evolutionary constraints are looser. (cnrs.fr)
  • An analysis of 7670 transcription start sites showed that roughly 40% had an exact match to the BBCA+1BW Inr sequence. (wikipedia.org)
  • Because these technologies digitally tabulate the sequence of many individual DNA fragments, unlike conventional techniques which simply report the average genotype of an aggregate collection of molecules, they offer the unique ability to detect minor variants within heterogeneous mixtures [2]. (justia.com)
  • The amino acid sequence of mature Coprisin was found to be 79.1% and 67.4% identical to those of defensin-like peptides of Anomala cuprea and Allomyrina dichotoma , respectively. (hindawi.com)
  • The amino acid sequence of the homeodomain of Hox-1.11 is identical to that of Hox-2.8, and the N-terminal and C-terminal regions of Hox-1.11 are similar to those of human HOX2H, which is the equivalent of murine Hox-2.8. (nih.gov)
  • Although Inr element varies between promoters, the sequence is highly conserved between humans and yeast. (wikipedia.org)
  • Their consensus (average) sequences, deduced from analyses of many promoters (Figure 28. (forextrading-madeeasy.com)
  • Here we demonstrate that these parallels extend to the immune response of Drosophila. (cnrs.fr)
  • Collections of Drosophila and their relatives were performed using bait traps on the campus of Saint Joseph's University, in Philadelphia and Lower Merion, Pennsylvania, between July and December 2011 and continuing in March of 2012. (researchgate.net)
  • 9012, Dec 90) DROSOPHILA Listing of Molecular Biology Databases, LiMB (Rel. (bio.net)
  • Through molecular biology techniques, the pentapeptide sequence, molecular weight, and architecture of the ELP can be precisely controlled for subsequent an purified as a recombinant polypeptide, resulting in monodisperse, non-immunogenic (Urry et al. (justia.com)
  • Thus, origins in multicellular eukaryotes are more similar to those in S. pombe than to those in S. cerevisiae in terms of their size, sequence complexity, and their lack of a required consensus sequence. (nih.gov)
  • Updates to Data Collections ------------------------------------ tRNA - We have added a compilation of tRNA sequences from Mathias Sprinzl at the University of Bayreuth, Germany. (bio.net)
  • Allan Human Brain Atlas: BrainSpan (Atlas of the Developing Brain) - An atlas describing the transcriptional mechanism of human brain development, which includes RNA sequencing and exon microarray data profiling up to 16 cortical and subcortical structures across the full course of human brain development. (nih.gov)
  • The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. (lookformedical.com)
  • Amplifying the rec A gene allowed unambiguous determination of the sequence of a 336-bp fragment for all isolates. (cdc.gov)
  • Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. (justia.com)
  • To investigate the possibility of a clonal diffusion in lice, the rec A gene sequence of isolates was determined and compared to that of the collection and strains. (cdc.gov)
  • Hemagglutinin (HA) amino acid numbering was based on the mature HA protein sequence after removal of the signal peptide. (cdc.gov)