Dopamine and camp regulated phosphoprotein 32. Medical search. Frequent questions

Genetic inactivation either of dopamine D 2 receptors or of adenosine A 2A receptors reduces the phosphorylation of DARPP-32 at Thr34 and the motor depression produced by CP55,940. (jneurosci.org)
Our data indicate that a considerable proportion of the psychomotor effect of cannabinoids can be accounted for by a signaling cascade in striatal projection neurons involving PKA-dependent phosphorylation of DARPP-32, achieved via modulation of dopamine D 2 and adenosine A 2A transmission. (jneurosci.org)
A considerable proportion of the psychomotor effects of cannabinoids can be accounted for by a signaling cascade in striatal projection neurons involving PKA-dependent phosphorylation of DARPP-32, achieved via modulation of dopamine D2 and adenosine A2A transmission. (wikipedia.org)
DARPP-32, a dopamine- and adenosine 3',5'-monophosphate (cAMP)-regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)-facilitated sexual receptivity in female rats and mice. (cdc.gov)
Ontogeny of the dopamine and cyclic adenosine-3':5'-monophosphate-regulated phosphoprotein (DARPP-32) in the pre- and postnatal mouse central nervous system. (cornell.edu)
Dopamine- and adenosine-3',5'-monophosphate (cAMP)-regulated phosphoprotein of 32 kDa (DARPP-32) in the adrenal gland: immunohistochemical localization. (cornell.edu)
Dopamine- and adenosine-3':5'-monophosphate (cAMP)-regulated phosphoprotein of Mr 32,000 (DARPP-32) in the retina of cat, monkey and human. (cornell.edu)
Identification of a dopamine- and 3'5'-cyclic adenosine monophosphate-regulated phosphoprotein of 32 kD (DARPP-32) in parathyroid hormone-producing cells of the human parathyroid gland. (cornell.edu)

DARPP-32 (dopamine- and cAMP-regulated phosphorprotein, Mr = 32,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is an inhibitor of protein phosphatase-1 and is enriched in dopaminoceptive neurons possessing the D1 dopamine receptor. (cornell.edu)
Phosphorylated Mr 32,000 dopamine- and cAMP-regulated phosphoprotein inhibits Na+,K(+)-ATPase activity in renal tubule cells. (cornell.edu)
Synthetic peptide analogs of DARPP-32 (Mr 32,000 dopamine- and cAMP-regulated phosphoprotein), an inhibitor of protein phosphatase-1. (cornell.edu)
1995. DARPP-32 (dopamine and cAMP-regulated phosphoprotein, M(r) 32,000) is a membrane protein in the bovine parathyroid. . (cornell.edu)
Interleukin (IL)-1ß, IL-6, IL-10, tumour necrosis factor-α, dopamine-cAMP-regulated phosphoprotein of 32,000 kDa (DARPP-32) and neuronal calcium sensor (NCS-1) levels were evaluated in the frontal cortex, hippocampus and striatum. (bvsalud.org)

Here, we report that the motor depressant effect produced by the cannabinoid receptor agonist (-)-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]trans-4-(3-hydroxypropyl)cyclohexanol (CP55,940) is attenuated by genetic inactivation of the dopamine- and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32), which is abundantly expressed in the medium spiny neurons of the striatum. (jneurosci.org)
In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. (wikipedia.org)
A key component of these neurons is the protein DARPP-32, which receives and processes various types of dopamine and glutamate inputs and translates them into specific biochemical, cellular, physiological, and behavioral responses. (nih.gov)
1999. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons. . (cornell.edu)

Protein phosphatase 1 regulatory subunit 1B (PPP1R1B), also known as dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP-32), is a protein that in humans is encoded by the PPP1R1B gene. (wikipedia.org)
Also, neuronal calcium sensor-1 was suggested to modulate the expression of DARPP-32. (wikipedia.org)

Activation of CB1 receptors, either by an agonist or by inhibition of reuptake of endogenous cannabinoids, stimulates phosphorylation at Thr34, thereby converting DARPP-32 into an inhibitor of protein phosphatase-1. (jneurosci.org)

The endocannabinoid system has been shown to exert both negative and positive control on striatal dopamine D 2 receptor transmission. (jneurosci.org)
The function of DARPP32 seems to be regulated by receptor stimulation. (wikipedia.org)
Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. (wikipedia.org)
These increases were not inhibited by a D1 subclass dopamine receptor antagonist. (cdc.gov)
DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice. (cdc.gov)

Purified bovine DARPP-32 was phosphorylated in vitro by casein kinase II to a stoichiometry greater than 2 mol of phosphate/mol of protein whereas two structurally and functionally related proteins, protein phosphatase inhibitor-1 and G-substrate, were poor substrates for this enzyme. (cornell.edu)
Phosphorylation by casein kinase II did not affect the potency of DARPP-32 as an inhibitor of protein phosphatase-1, which depended only on phosphorylation of Thr34 by cAMP-dependent protein kinase. (cornell.edu)
this is disputed by more recent research that claims cAMP signaling induces dephosphorylation of DARPP32) phosphorylated DARPP32 is a potent protein phosphatase-1 (PPP1CA) inhibitor. (wikipedia.org)
Distribution of protein phosphatase inhibitor-1 in brain and peripheral tissues of various species: comparison with DARPP-32. (cornell.edu)

Acute exposures to 100 or 200 mg/kg ATR given intraperitoneally to evaluate potential mechanisms reduced both basal and potassium-evoked striatal dopamine release. (nih.gov)

This gene is also known as DARPP-32, highlighting its role as a dopamine- and cyclic AMP-regulated phosphoprotein. (wikipedia.org)
Dopamine and cyclic-AMP activated Mr32kDa phosphoprotein (DARPP-32) is a central signaling proteins in neurotransmission. (pp1a.com)

One well studied target for the actions of dopamine is DARPP32. (wikipedia.org)
SCH 58261 selectively inhibited the ischemia-induced phosphorylation of NR1, Na +,K +-ATPase, and cAMP-regulated phosphoprotein 32 KDa (DARPP32) at PKA-sensitive sites at 3 hours of recovery and improved Na +,K +-ATPase activity. (johnshopkins.edu)

In the striatum, the major component of the basal ganglia, CB1 receptors regulate neurotransmission in various manners. (jneurosci.org)
Secondly, a comprehensive perturbation analysis identified the specific role of each component on the system's signal transduction ability.Our study investigated the effects of various patterns of neurotransmission on signal integration and interpretation by DARPP-32 and showed that the DARPP-32 system has the capability of discerning surprisingly many neurotransmission scenarios. (nih.gov)

Point mutation of Thr34, the protein kinase A (PKA) phosphorylation site of DARPP-32, produces a similar reduction in the effect of the CB1 agonist. (jneurosci.org)
However, phosphorylation of DARPP-32 by casein kinase II facilitated phosphorylation of Thr34 by cAMP-dependent protein kinase with a 2.2-fold increase in the Vmax and a 1.4-fold increase in the apparent Km. (cornell.edu)
P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. (cdc.gov)
It regulates the activities of PROTEIN PHOSPHATASE-1 and PROTEIN KINASE A , and it is a key mediator of the biochemical, electrophysiological, transcriptional, and behavioral effects of DOPAMINE . (bvsalud.org)
Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K(+)-ATPase and effects of site-directed mutagenesis. (cornell.edu)

In slices of rat caudate-putamen prelabeled with [32P]phosphate, DARPP-32 was phosphorylated on seryl residues under basal conditions. (cornell.edu)

This, in turn, prevents dephosphorylation of downstream target proteins, thereby amplifying the effects produced by activation of the cAMP/PKA cascade ( Greengard, 2001 ). (jneurosci.org)

Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. (cdc.gov)

Its endogenous activators include: low pH (acidic conditions), the endocannabinoid anandamide , N-oleyl-dopamine, and N-arachidonoyl-dopamine . (wikipedia.org)

Phosphorylation of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein, by casein kinase II. (cornell.edu)
Sequencing of chymotryptic and thermolytic phosphopeptides from bovine DARPP-32 phosphorylated by casein kinase II suggested that the main phosphorylated residues were Ser45 and Ser102. (cornell.edu)
Casein kinase II phosphorylated DARPP-32 with an apparent Km value of 3.4 microM and a kcat value of 0.32 s-1. (cornell.edu)
In vitro, DARPP-32 phosphorylated by casein kinase II was dephosphorylated by protein phosphatases-1 and -2A. (cornell.edu)
Phosphorylation of DARPP-32 by casein kinase II in intact cells may therefore modulate its phosphorylation in response to increased levels of cAMP. (cornell.edu)
Characterization in mammalian brain of a DARPP-32 serine kinase identical to casein kinase II. (cornell.edu)

ARPP-21, a cAMP-regulated phosphoprotein enriched in dopamine-innervated brain regions: tissue distribution and regulation of phosphorylation in rat brain. (cornell.edu)
These findings highlight potential unappreciated roles for AMPK in regulating tissue glycogen dynamics and expand AMPK's known roles in exercise and metabolism. (edu.au)

In contrast, point mutation of Thr75, a site on DARPP-32 specifically phosphorylated by cyclin-dependent kinase 5, does not affect the behavioral response to CP55,940. (jneurosci.org)

P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. (cdc.gov)

Description: Potentiator potency: Increase of cAMP by LY3154207 in the presence of an EC 20 concentration of dopamine in HEK293 cells transiently expressing human D 1 receptors. (guidetomalariapharmacology.org)
Description: Potentiator potency: Increase of cAMP by LY3154207 in the presence of an EC 20 concentration of dopamine in HEK293 cells transiently expressing mouse D 1 receptors. (guidetomalariapharmacology.org)

In the case of rat DARPP-32, the identification of these phosphorylation sites was confirmed by manual Edman degradation. (cornell.edu)

This alteration is suggested to be related to the pathology, since antipsychotics do not regulate the expression of DARPP-32. (wikipedia.org)
Brain-derived neurotrophic factor regulates the expression of DARPP-32. (wikipedia.org)
Moreover, mutant huntingtin expression interferes with projection neuron migration as normal huntingtin regulates Rab11-mediated N-cadherin trafficking [ 5 ]. (biomedcentral.com)
ERG may regulate these changes by altering expression of the histone deacetylase, HDAC1, as this is one of the most up-regulated genes in ERG-positive prostate cancer. (biomedcentral.com)

in which it acts as a modulator of the cAMP pathway. (jneurosci.org)

a group of subcortical structures that regulate motor behavior. (jneurosci.org)

3. Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating p68 /DEAD box protein 5. (nih.gov)

DARPP-32 levels are decreased in the dorsolateral prefrontal cortex and lymphocytes of both schizophrenia and bipolar disorder patients. (wikipedia.org)
Furthermore, in vivo studies revealed that J3 decreased T-HTT and misfolded protein levels in the striatum and increased the levels of the medium spiny neuron marker DARPP-32. (biomedcentral.com)

A phosphoprotein that was initially identified as a major target of DOPAMINE activated ADENYLYL CYCLASE in the CORPUS STRIATUM . (bvsalud.org)

DARPP-32 is critical for dopamine dependent striatal synaptic plasticity, possibly by serving as a dopamine-dependent gating mechanism for calcium/CaMKII signaling. (wikipedia.org)
Protein phosphatase 2A (PP2A) regulate critical cell signaling and the human tumor suppressor. (pp1a.com)

Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). (nih.gov)

Our results suggest that PP2A-PR72 plays an important role in regulating cardiac contractile function and Ca 2+ cycling, indicating that the upregulation of PR72 in heart failure is an attempt to compensate functionally. (frontiersin.org)

We also screened out potential mechanisms underlying this capability of the DARPP-32 system. (nih.gov)

The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. (nih.gov)

Anatomy32

Organisms15

Diseases7

Chemicals and Drugs145

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Psychiatry and Psychology7

Phenomena and Processes26

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Health Care1

Adenosine8

32,0005

Neurons4

Neuronal2

Converting DARPP-321

Receptor5

Inhibitor4

Striatal dopamine1

Cyclic2

DARPP322

Neurotransmission2

Protein kinase5

Caudate-putamen1

PROTEINS1

Gene1

Anandamide1

Casein6

Tissue2

Behavioral1

Hypothalamus1

Potency2

Identification1

Expression4

Pathway1

Structures1

Migration1

Levels2

Target1

Critical2

Activity1

Important1

Potential1

Formation1