Domain methyltransferases. Medical search. Frequent questions

SET domain protein lysine methyltransferases (PKMTs) regulate transcription and other cellular functions through site-specific methylation of histones and other substrates. (rcsb.org)
PWWP domains of the de novo DNA methyltransferases DNMT3A and DNMT3B read this epigenetic mark to guide DNA methylation. (nih.gov)
One of the modifications called methylation is conducted by a family of SET-domain containing proteins called NSD1, NSD2, and NSD3. (edu.sa)
Noma, K., Allis, C.D. & Grewal, S.I. Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries. (nature.com)
To explore the effect of DNA methyltransferase, demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and proto-oncogene in human gastric cancer. (wjgnet.com)
The code is an array of post-translational modifications (acetylation, phosphorylation, methylation, ubiquitination, and sumoylation) of NH 2 -terminal tails of core histone and to a lesser degree their globular domains. (aacrjournals.org)
The effects of DNA methylation and the histone code are due, at least in part, to modification-specific recruitment of factors, such as heterochromatin-associated proteins (HP1) and methyl-binding domain proteins, which establish and maintain higher order of chromatin structure. (aacrjournals.org)
Lysine methylation marks manifest their biological effect via so-called 'readers' (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. (europa.eu)
Importantly, the KMET-READ project will also develop a yeast-3-hybrid method for the identification of new reading domains, which will allow to discover binding partners for just recently characterized new protein methylation marks. (europa.eu)

The trimethylated side chain of Lys36 (H3K36me3) is inserted into an aromatic cage similar to the "Royal" superfamily domains known to bind methylated histones. (nih.gov)
Belongs to the class V-like SAM-binding methyltransferase superfamily. (abcam.com)

Various studies have found that post-SET domain and basic post-SET extension of NSD proteins are crucial for nucleosome interactions and for conducting enzymatic reactions. (edu.sa)
Similar methyltransferase proteins are found in other highly infectious bacteria, including the pathogen Mycobacterium tuberculosis that causes Tuberculosis, a disease that results in more than 1 million deaths annually. (sciencedaily.com)
Oxr1p belongs to the group of TLDc domain containing proteins that are highly conserved in higher eukaryotes. (upstate.edu)
These proteins contain two related domains known as the chromo- and the chromoshadow-domain. (cipsm.de)
We recently showed that a member of the mitochondrial transcription termination factor (MTERF) family of proteins, MTERF4, recruits NSUN4, a 5-methylcytosine RNA methyltransferase, to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. (rcsb.org)
Alpha-N-methyltransferase methylates the N terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. (embl.de)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)
It is also found with other divergent Sm domains and in proteins such as Dcp3p and FLJ21128, where it is found N terminal to the YjeF-N domain, a novel Rossmann fold domain PMID 15257761. (embl-heidelberg.de)
There are 3917 FDF domains in 3915 proteins in SMART's nrdb database. (embl-heidelberg.de)
Taxonomic distribution of proteins containing FDF domain. (embl-heidelberg.de)
The complete taxonomic breakdown of all proteins with FDF domain is also avaliable . (embl-heidelberg.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing FDF domain in the selected taxonomic class. (embl-heidelberg.de)
Sm and Sm-like proteins of the Lsm (like Sm) domain family are generallyinvolved in essential RNA-processing tasks. (embl-heidelberg.de)
While recent research hasfocused on the function and structure of small family members, little isknown about Lsm domain proteins carrying additional domains. (embl-heidelberg.de)
Using anintegrative bioinformatics approach, we discovered five novel groups ofLsm domain proteins (Lsm12-16) with long C-terminal tails and investigatedtheir functions. (embl-heidelberg.de)
Lsm16 proteins have an additional C-terminal YjeF_N domain of asyet unknown function. (embl-heidelberg.de)
Novel conserved domains in proteins with predicted roles in eukaryotic cell-cycleregulation, decapping and RNA stability. (embl-heidelberg.de)
Reading domains include Plant homeodomains (PHD) and Chromodomains (CD) found in many chromatin proteins. (europa.eu)
Since protein domains are representatives of functional regions within proteins, mutations on them may disturb the protein functionality. (biomedcentral.com)
Subsequently, cancer type-specific domains facilitated the identification of specific proteins for each cancer type. (biomedcentral.com)
Purification of individual SARS-CoV-2 proteins from human cells have identified a potential interaction between a catalytic-inactive version of Nsp5 with human tRNA methyltransferase 1 (TRMT1) ( Gordon et al , 2020b ). (elifesciences.org)

SET domain protein methyltransferases catalyze the transfer of methyl groups from the cofactor S -adenosylmethionine (AdoMet) to specific lysine residues of protein substrates, such as the N-terminal tails of histones H3 and H4 and the large subunit of the Rubisco holoenzyme complex. (nature.com)
The crystal structures of pea Rubisco large subunit methyltransferase (LSMT) in ternary complexes with either lysine or ε- N -methyllysine (MeLys) and the product S -adenosylhomocysteine (AdoHcy) were determined to resolutions of 2.65 and 2.55 Å, respectively. (nature.com)
Klein, R.R. & Houtz, R.L. Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N -methyltransferase. (nature.com)

2010 ). PRDM9 includes several functional domains, such as a DNA-binding zinc finger array, a histone lysine methyltransferase PR/SET (PRDI-BF1 and RIZ1 homology) domain, and a Krüppel-associated box (KRAB)-related domain. (springer.com)

KMET-READ plans to investigate the biological role of these reading domains in essential histone lysine methyltransferases - PHDs in MLL2 and MLL3 and CDs in SUV39H1 and SUV39H2. (europa.eu)

Predicted to enable tRNA (guanine) methyltransferase activity. (nih.gov)
tRNA (cytidine(34)-2'-O)-methyltransferase. (cathdb.info)
Here, we show that Nsp5 binds and cleaves human tRNA methyltransferase 1 (TRMT1), a host enzyme required for a prevalent post-transcriptional modification in tRNAs. (elifesciences.org)
These results will be of interest to virologists interested in studying the alterations in tRNA modifications, host methyltransferases, and viral infections. (elifesciences.org)

Putative domains in ORF1. (cdc.gov)
Recently, we identified a putative N7-MTase domain in the ORF1a region (N7-MT-1a) of certain members of the large genome Tobaniviridae family. (hal.science)

Based on this structure, we propose a model of the DNMT3A PWWP domain-H3K36me3 complex and build a model of DNMT3A (PWWP-ADD-CD) in a nucleosomal context. (nih.gov)
Tatton-Brown-Rahman syndrome (TBRS) is an overgrowth disorder caused by germline heterozygous mutations in the DNA methyltransferase DNMT3A. (haematologica.org)

The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)

While 2′-O-MTases can be identified for most other families based on conservation of both sequence motifs and genetic loci, identification of the N7-guanine methyltransferase has proved more challenging. (hal.science)
MTERF4 is composed of structurally repeated MTERF-motifs that form a nucleic acid binding domain. (rcsb.org)
All of them are evolutionarily conserved in eukaryoteswith an N-terminal Lsm domain to bind nucleic acids followed by as yetuncharacterized C-terminal domains and sequence motifs. (embl-heidelberg.de)
Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. (lu.se)

To elucidate the mechanism underlying this switch, we have characterized a Phe/Tyr switch mutant of the histone H4 Lys-20 (H4K20) methyltransferase SET8, which alters its specificity from a monomethyltransferase to a dimethyltransferase. (rcsb.org)
SET8 (also known as PR-Set7 and SETD8) is a histone methyltransferase that monomethylates histonfe H4-K20. (rupress.org)

Here we report the first crystal structure of the DNMT3B PWWP domain-H3K36me3 complex. (nih.gov)

This is a PFAM domain. (embl.de)
This study has provided researchers with a publicly available data repository for studying both CATH and Pfam domain regions on protein-coding genes. (biomedcentral.com)
Beside analyzing Pfam protein domains as sequence-based domains, CATH protein domains have also been studied as structure-based domains, which were not included in relevant studies to this date. (biomedcentral.com)

NMR results showed that the post-SET domain extension is required for DNA to bind to NSD2 and NSD3 methyltransferases. (edu.sa)
NSD3 is a histone H3 methyltransferase that plays an important role in chromatin biology. (a-star.edu.sg)
A construct containing the methyltransferase domain encompassing residues Q1049-K1299 of human NSD3 was obtained and biochemical activity was demonstrated using histone as a substrate. (a-star.edu.sg)

The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)
The proposal will apply an interdisciplinary approach in an international environment to maximize its chances of success: the biological role of reading domains will be evaluated with molecular biology (histone and chromatin pulldowns, ChIP-seq, confocal microscopy), biochemistry (Peptide arrays, mass spectrometry, methyltransferase activity assays) and biophysics techniques (fluorescence anisotropy, circular dichroism spectroscopy) as well as crystallography (solving the structure of reading domains). (europa.eu)

The methyltransferase SETD2 regulates cryptic transcription, alternative splicing, and the DNA damage response. (frontiersin.org)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)

Phase 1 study of JNJ-64619178, a protein arginine methyltransferase 5 inhibitor, in patients with lower-risk myelodysplastic syndromes. (cdc.gov)

The methyltransferase protein also was found to have an ortholog, or similar counterpart, in human DNA. (sciencedaily.com)

Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 is a protein that in humans is encoded by the PCMTD1 gene. (wikipedia.org)

Interestingly, a protein-coding splice variant of SETD2 lacks the extended N-terminus but contains the catalytic SET domain as per the ENSEMBL database. (frontiersin.org)

Demethylmenaquinone Methyl Transferase Is a Membrane Domain-Associated Protein Essential for Menaquinone Homeostasis in Mycobacterium smegmatis . (bvsalud.org)

Methyltransferase is a potential virulence factor in this pathogen, which causes Tularemia, an infection common in wild rodents, especially rabbits, that can be transmitted to humans though bites, touch, eating or drinking contaminated food or water, or even breathing in the bacteria. (sciencedaily.com)

Belongs to the C5-methyltransferase family. (lu.se)

Regulation of chromatin structure by site-specific histone H3 methyltransferases. (nature.com)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)

Therefore, studying mutations at domain level may point researchers to more accurate assessment of the functional impact of the mutations. (biomedcentral.com)
This article presents a comprehensive study to map mutations from 29 cancer types to both sequence- and structure-based domains. (biomedcentral.com)
Statistical analysis was performed to identify candidate domains in which mutations occur with high statistical significance. (biomedcentral.com)
To this aim, some efforts have been made to study cancer mutations at the protein domain level. (biomedcentral.com)
12 ] mapped non-synonymous somatic mutations of Breast Invasive Carcinoma and Colon Adenocarcinoma Tumor samples to their corresponding protein domains, in order to extract domains with significant mutation frequency. (biomedcentral.com)

It is found at the C terminus of Scd6p-like SM domains. (embl-heidelberg.de)
The identification of an additionalmethyltransferase domain at the C-terminus of one of the Lsm12 proteinsalso led to the recognition of three new groups of methyltransferases,presumably dependent on S-adenosyl-l-methionine. (embl-heidelberg.de)

RdRp, RNA-dependent RNA polymerase domain. (cdc.gov)
This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent trimethylation of histone H3 at lysine 4. (springer.com)

Human TRMT112-Methyltransferase Network Consists of Seven Partners Interacting with a Common Co-Factor. (nih.gov)
Using genomic sequencing, the spelling out of billions of genetic instructions stored in DNA, the study identified several methyltransferase protein families that are very similar in otherwise very distantly related human bacterial pathogens. (sciencedaily.com)
The study also identified distinct methyltransferase subtypes in human pathogens such as Coxiella, Legionella, and Pseudomonas. (sciencedaily.com)
PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)

Although the overall sequence of the orthologs is highly similar, the study identifies a protein domain carrying distinct amino acid variations present in the different organisms. (sciencedaily.com)
No obvious hydrophobic signal sequence, membrane-spanning domains, or potential N-glycosylation sites were found in this sequence. (lu.se)

Predicted to enable protein-L-isoaspartate (D-aspartate) O-methyltransferase activity. (nih.gov)
Here, we demonstrate that this domain indeed harbors N7-specific methyltransferase activity. (hal.science)
However, mitoribosome biogenesis does not depend on the methyltransferase activity of MRM2. (nature.com)

In its absence, mtLSU particles (visualized by cryo-EM at the resolution of 2.6 Å) present disordered RNA domains, partial occupancy of bL36m and bound MALSU1:L0R8F8:mtACP anti-association module, allowing five mtLSU biogenesis intermediates with different intersubunit interface configurations to be placed along the assembly pathway. (nature.com)

Researchers found methyltransferase in the pathogen Francisella tularensis subspecies tularensis, the most virulent form of Francisella. (sciencedaily.com)

Identification and characterization of SET domain family genes in bread wheat (Triticum aestivum L. (nature.com)

We present the crystal structure ofthe DDX6 C-terminal RecA-like domain bound to a highly conserved FDF sequencemotif in the decapping activator EDC3. (embl-heidelberg.de)

NSUN4 lacks an N- or C-terminal extension that is commonly used for RNA recognition by related RNA methyltransferases. (rcsb.org)

In this thesis, I attempt to define the role of post-SET domain basic extension on DNA binding, using nuclear magnetic resonance and isothermal titration calorimetry. (edu.sa)
In particular, RNA methyltransferases play a central role in mRNA stability and immune escape. (hal.science)

Anatomy16

Organisms19

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes83

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science5

Health Care1

Methylation9

Superfamily2

Proteins21

Subunit3

Histone lysine methyltransferase1

Lysine methyltransferases1

TRNA4

Putative2

DNMT3A2

Binds1

Motifs4

SET82

PWWP1

Pfam3

NSD33

Peptide2

Transcription2

Inhibitor1

Ortholog1

Gene1

Catalytic1

Mycobacterium1

Humans1

Belongs1

Chromatin structure2

Mutations5

Terminus2

Dependent2

Human4

Sequence2

Activity3

Biogenesis1

Researchers1

Characterization1

Highly conserved1

Recognition1

Role2