Differentiation growth applications cells cultured. Medical search. Frequent questions

Efficient in vitro expansion of MSCs is however necessary for their clinical application. (hindawi.com)
However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
Therefore, significant efforts have been made toward the development of more realistic three-dimensional (3D) in vitro cell culture models that would better mimic tissue physiology. (srce.hr)
Accelerated uptake of ESC based cell therapy on in vivo and in vitro principle is expected to fuel growth through to 2024. (grandviewresearch.com)
In vitro investigation of human umbilical vein endothelial cell (HUVEC) and adipose-derived stem cell (ADSC) responses in a biomimetic 3D bioprinted blood vessel model under complex physiological flows was performed. (aps.org)
This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
ReNcell NSC Maintenance Medium (SCM005): ReNcell Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. (thomassci.com)
Histological, RT-qPCR, western blot analyses and steroid hormone measurements were performed on in vitro cultured mouse prepubertal testicular tissues and age-matched in vivo controls. (elifesciences.org)
In vitro spermatogenesis could indeed be proposed to patients with testicular localization of residual tumor cells, for whom testicular tissue autografting is not indicated (about 30% of patients with acute leukemia). (elifesciences.org)
It has been previously shown that supplementing organotypic culture media with retinol improves the in vitro production of spermatids and spermatozoa in prepubertal mouse testicular tissues cultured at a gas-liquid interphase ( 5 , 7 ). (elifesciences.org)
A decline in intratesticular testosterone levels has been highlighted in vitro in 35- to 49-day cultures of 5 d pp mouse testes, i.e. after the end of the first wave of spermatogenesis ( 9 ). (elifesciences.org)
However, it is still unclear to what extent hiPSC may be capable of recapitulating in vitro the cellular and molecular features of the native retina, especially regarding photoreceptor differentiation and functional maturation. (nature.com)
These polymeric materials organise fibronectin (FN), an important protein of the extracellular matrix, and sequester rhBMP-2 in synergy with the integrin binding region to direct stem cell differentiation in vitro. (interface-online.org.uk)
The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
Cell culture protein surface coating is a process, which aims to improve the growth and cell adhesion of in vitro cell culture. (kbvresearch.com)
Protein surface coating of cell culture comprises coating the cell culture surface using extracellular matrix and protein components to enhance the proliferation and adherence of cells in vitro. (kbvresearch.com)
The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. (utwente.nl)
Different groups have established several protocols to induce the differentiation of MSCs into HLCs in vitro . (springer.com)
This protocol provides researchers with the tools to culture and assess purity and adipogenic differentiation capacity of human ASCs, which can then be utilized for required downstream in vitro applications. (bio-protocol.org)
The isolated human ASCs show high purity and demonstrate adipogenic differentiation capacity in vitro . (bio-protocol.org)
Here we provide a comprehensive protocol on how to isolate and culture individual cell types from whole endometrial tissues for use in in vitro experiments. (bio-protocol.org)
Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. (bio-protocol.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
The comparative results of different scaffolds with the parameters of aperture, porosity, water absorption and degradation rate show that the volume ratio of 7:3 is more suitable for cell culture in vitro and the inoculation rate of NSCs is markedly improved in LN-treated scaffolds. (ox.ac.uk)
Collagen I is used in both 2D and 3D cell culture to make the in vitro cellular environment more in vivo -like. (thermofisher.com)
A well-defined in vitro three-dimensional culture of human endometrium and its applicability to endometrial cancer invasion. (thermofisher.com)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Our approach is to first understand how hESC interact with extracellular matrix materials by analyzing candidate adhesive substrate proteins and integrin receptors that mediate attachment, survival, proliferation and differentiation. (ca.gov)
in this study the effect of alginate hydrogel architecture was investigated by comparing rat bone marrow cell proliferation and differentiation on calcium cross linked sodium alginate discs and 1mm internal diameter tubes. (ox.ac.uk)
Through the observation of laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM), NSCs within collagen-chitosan composite scaffolds present good growth, proliferation and differentiation. (ox.ac.uk)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
Mesenchymal stem cell medium was used in the control groups. (scielo.br)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. (bio-protocol.org)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

Furthermore, as these are biocompatible, the resulting suspension can be used for delivery of cell therapies in vivo. (wikipedia.org)
Materials and methods: Two-dimensional (2D) cell cultures, in comparison with original tissues, does not fully reproduce in vivo cell growth and differentiation. (srce.hr)
Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. (nature.com)
In addition to isolating single cells in order to determine if true stem cells are present in adult brain tissue, Gage also plans to transplant recovered cells into animals to test if the cells can survive and differentiate in vivo. (salk.edu)
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
In drug discovery and tissue engineering, interest in three-dimensional (3D) cell culture techniques have increased due to their obvious advantages in giving more physiologically relevant data and more predictive statistics for in vivo studies. (kbvresearch.com)

Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA. (jneurosci.org)
The new system also provides a valuable platform for fundamental studies of the culture microenvironment in stem cell biology. (genengnews.com)
Stem cells are extremely sensitive to such changes in pH, and this leads to poor reproducibility in manual culturing, representing a major obstacle to both furthering our understanding of stem cell biology and the commercialization of stem cell applications. (genengnews.com)
They will be joined by leaders in stem cell biology, cancer, epigenetics and computational systems analysis. (ca.gov)
Four independent Center-Initiated Projects (CIPs) have been proposed, encompassing studies ranging from basic stem cell biology and differentiation, to investigations of disease mechanism and development of novel technologies for analysis of genome function. (ca.gov)
The cell composition and arrangement of the epithelium make intestinal organoids useful for studying intestinal cell biology, regeneration, differentiation, as wells as diseases phenotypes including effects of specific mutations, microbiome, or inflammation process. (moleculardevices.com)
The cells derived from these tissues constitute a model system for studying the brain cell biology of these disorders, according to Gage. (salk.edu)
Complying with the stem cell biology information and the most recent technological advances, stem cell culture conditions are crucial and must be continuously optimized. (kbvresearch.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The most commonly known use of heparin in cell biology is its application in stem cell cultures. (ashpublications.org)
Cell Applications, Inc. cell biology products and custom services support basic life science in the world's top universities and research institutes, as well as drug discovery and development in leading pharma on projects like tissue engineering, spheroid generation and protocol development. (probioscience.org)
Scientists routinely cite Cell Applications, Inc. cells, media and cell biology products, including articles and patents for Nobel-awarded breakthroughs. (probioscience.org)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)

Stem cells derived through such techniques can be converted into liver, nerve, and heart tissues. (grandviewresearch.com)
Applicability for inducing differentiation into a wide range of tissues is attributive to the largest share of regenerative medicine. (grandviewresearch.com)
The organotypic culture procedure, which preserves testicular tissue architecture, microenvironment and cell interactions, has been used successfully to obtain spermatozoa from fresh or frozen/thawed mouse prepubertal testicular tissues ( 3 - 6 ). (elifesciences.org)
Following injury or disease, an organism undergoes a variety of (natural or induced) processes to direct cells, tissues and cellular processes towards healing and eventually regeneration. (interface-online.org.uk)
Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
Stem cells are the source of all bodily tissues and comprehending their features facilitates comprehension of the development and homeostasis of the healthy and diseased body. (kbvresearch.com)
A rising number of studies and research indicate that in contrast to the 2D culture system, 3D cell culture systems more effectively replicate the actual environment in which cells live in tissues. (kbvresearch.com)
Collagen I is the most common fibrillar collagen for cell culture and is found in skin, bone, tendons, and other connective tissues. (thermofisher.com)
Tissues and Cells. (lu.se)

ReNcell NSC Freezing Medium (SCM007): ReNcell NSC Freezing Medium is qualified for use with ReNcell immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell NSC Maintenance Medium (CHEMICON Catalog No. SCM005). (thomassci.com)
This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. (utwente.nl)
Quiescent keratocytes cultured in serum-free media were exposed to various concentrations of MMC and then treated with transforming growth factor-β (TGFβ). (molvis.org)
We demonstrated that the UROtsa cells could be successfully placed into a serum-free growth medium consisting of a 1:1 mixture of Dulbeco's modified Eagle's medium and Ham's F-12 supplemented with selenium (5 ng/mL), insulin (5 µg/mL), transferrin (5 µg/mL), hydrocortisone (36 ng/mL), triiodothyronine (4 pg/mL), and epidermal growth factor (10 ng/mL). (archive.org)
Under serum-free growth conditions, confluent UROtsa cells were shown by light microscopy to produce raised, three-dimensional structures. (archive.org)
The findings suggest that the UROtsa cells grown with a serum-free medium could be a valuable adjunct for studying environmental insult to the human urothelium in general and for the stress response in particular. (archive.org)

The differentiated cells are further used for therapeutic applications in regenerative medicine. (grandviewresearch.com)
The applications of SCs in regenerative medicine are limited because of slow growth rate and difficulties in harvesting. (scirp.org)
The regenerative medicine bioprocessing program within the UCL department of biochemical engineering focuses on two areas: regenerative medicine translation and bioprocess engineering of stem cells. (genengnews.com)
This research program will lead the way toward development of the safe use of stem cells in regenerative medicine. (ca.gov)
Recently, MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment. (techscience.com)
Conferring cell fitness by multi-target drug combinations may become a common approach in cryobiology, drug development, and regenerative medicine. (labroots.com)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)

Since heparan sulfate-proteoglycan is a major component of the extracellular matrix of skeletal muscle, factors that are bound to this proteoglycan, such as basic fibroblast growth factor (bFGF), are in a strategic position for neuronal signaling. (jneurosci.org)
Controlled differentiation that can be achieved in tissue committed cells through the co-culturing with basic fibroblast growth factor is expected to induce growth. (grandviewresearch.com)
These factors include FGF-2 (fibroblast growth factor 2) and cystatin C, which were added to the culture medium that bathed the cells. (salk.edu)
2. Primary cell culture: preparation of fibroblast culture from mouse skin. (pmf.hr)
Tanaka et al 5 showed that supplementation of heparin and fibroblast growth factor in stem cell cultures is crucial to maintaining proliferation and blocking terminal differentiation. (ashpublications.org)
F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
Macrophage-conditioned media was collected and applied to cultures of human pulmonary fibroblast line WI38-VA13 to induce myofibroblast transformation. (cdc.gov)
Induction of IL-1beta is dependent on the activation of the NLRP3 (NOD-like receptor family, pyrin domain containing 3) inflammasome and ROS (reactive oxygen species) production in macrophages, as inhibition of NLRP3 by MCC950 and reduction of ROS production by N-acetylcysteine blocked NLRP3 activation, IL-1beta induction, and fibroblast activation and differentiation. (cdc.gov)

In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

Cells may not differentiate properly without feedback from their environment, and attachment levels would be low. (wikipedia.org)
ReNcell CX Immortalized Cells (SCC007): ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. (thomassci.com)
I find it remarkable that we all have pockets of cells in our brains that can grow and differentiate throughout our lives and even after death," said Fred Gage , a professor at The Salk Institute and senior author of the study, which appears in the current Nature. (salk.edu)
Importantly, the recovered cells had the ability to differentiate into different types of brain cells. (salk.edu)
Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. (utwente.nl)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)

Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
Cultured cells are used for the manufacturing of biologics such as vaccines, therapeutic proteins and antibodies, and cell therapy products. (sigmaaldrich.com)
Although bacterial, yeast, and insect cell expressions systems are capable of overexpressing recombinant proteins, mammalian cells are most commonly used for biologics production due to their ability to propagate human viruses, express monoclonal antibodies, and incorporate post-translational modifications such as glycosylation that are critical to the production of effective biologics. (sigmaaldrich.com)
Several mammalian cell lines, including CHO, lymphoma (NS0, SP2/0) and human embryonic kidney (HEK) cells, have been developed and used to produce therapeutic proteins and antibodies at high levels. (sigmaaldrich.com)
First, we characterized the cell surface receptors for matrix proteins on stem cells and identified specific proteins that will support adhesion and growth of the cells. (ca.gov)
Second, using an interdisciplinary approach, we developed a novel method to screen peptides from these proteins for their ability to support adhesion and proliferation of the stem cells. (ca.gov)
Based on our characterization of cell surface receptors for matrix proteins on stem cells completed in the first year, we went on to test specific, purified proteins that will support adhesion and growth of the cells. (ca.gov)
Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
As part of the extracellular matrix, proteoglycans play important roles in cell signaling pathways by acting as molecular sponges, attracting molecules due to their charge and affinity to proteins. (ashpublications.org)
The basal expression patterns of the metallothionein (MT) and heat shock proteins 27, 60, and 70 were determined in these cells, and expression was in agreement with that known to occur for in situ urothelium. (archive.org)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)

Since endometrial stromal cells are easy to isolate, and expand rapidly from patients without leading to major ethical and technical problems. (scirp.org)
Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)
Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. (bio-protocol.org)
To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were co-cultured with allogeneic MSC or CM for one week followed by one week of culture in the absence of MSC. (bio-protocol.org)
To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage colony-stimulating factor (M-CSF). (bio-protocol.org)
To effectively support the growth and differentiation of primitive lymphohematopoietic progenitors, complex signals from stromal cells are important. (bio-protocol.org)
Several stromal cell lines are known to support both lymphoid and myeloid cells simultaneously in mouse. (bio-protocol.org)
In this protocol, we introduce two stromal co-culture systems for murine lymphohematopoietic progenitors and their application for limiting dilution assays. (bio-protocol.org)
Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. (bio-protocol.org)

To test this idea, we applied bFGF to cultured Xenopus spinal cord neurons and monitored the change in intracellular Ca2+ level with fura-2 ratio imaging. (jneurosci.org)
In one-third of the neurons, local application of bFGF elicited a 30-140% increase in intracellular Ca2+ level. (jneurosci.org)
Morphological Differentiation of Neurons on Microtopographic Substrates Fabricated by Rolled‐Up Nano. (researchgate.net)
This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. (jove.com)
It is particularly well suited for assaying synapses, which in human neurons require lengthy culture periods. (jove.com)
Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)
In addition to human iPSCs, it can be used to replate culture of primary neurons or to resuspend them for fact sorting or single cell sequencing. (jove.com)

Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
For regeneration applications the materials can be coated on bioabsorbable materials already commonly used in medical devices. (interface-online.org.uk)
Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)

Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS). (sigmaaldrich.com)
Traditional methods for manual cultivation of stem cells often lead to high variability and low consistency, making development of robust experimental protocols for stem cell research extremely difficult. (genengnews.com)
Methods for automated liquid handling were developed for cell seeding in Matrigel droplets and for media addition and exchanges. (moleculardevices.com)
Methods of quantitation and characterisations of the cells. (pmf.hr)
Methods of cell growth synchronisation. (pmf.hr)
5. methods of cell quantitation: cell counting, determination of the cell viability by differential staining, flow cytometry. (pmf.hr)
38th Astronomy's Phil Plait sent the people quickly on the possible trustworthy Book H-Transforms: Theory And Applications (Analytical Methods And Special Functions) 2004 . (souroujon.com)
This paper reports two general methods for extraction of cell-electrode electrical model parameters in cell culture (CC) assays. (csic.es)
Both of the proposed methods - minimization of system equations and data predictive model - search, during the experiment, the optimum values of the electrical model parameters employed for the electrode-cell model. (csic.es)
For the sake of experimental validation, we applied the proposed methods to practical CCs in cell growth assays with a cell line of AA8 Chinese hamster ovarian fibroblasts and the Oscillation Based Test technique for bioimpedance measurements. (csic.es)
These methods can be easily extrapolated to any general cell lines and/or other bioimpedance test methodologies. (csic.es)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
The taxonomic structure and stability of the bacterial communities occurring in these traditional sourdoughs were assessed using both culture-dependent and culture-independent methods. (who.int)

The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
their evolution from distinct cell lineages. (lu.se)

Conclusions: Although biological significance of obtained data from 2D and 3D cell cultures is still poorly understood, discrepancy of compunds activity illustrated importance of implementation 3D cell culture assays in early part of drug discovery process. (srce.hr)
Finally, deep insight information on cell size, number, and time-division can be extracted from the comparison with real cell culture assays in the future. (csic.es)
The presented approaches can be applied to CC assays based on electrical cell-substrate impedance spectroscopy (ECIS) technique for real-time supervision, providing the cell number per square centimeter, i.e., the cell density, as main result. (csic.es)
Using broth microdilution assays and cultures of M. ulcerans-infected macrophages, we found that atorvastatin, simvastatin and fluvastatin had antimicrobial activity against M. ulcerans. (bvsalud.org)

The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
We proposed to develop novel, well-defined, synthetic extracellular matrices that support survival and proliferation of human embryonic stem cells. (ca.gov)
We propose to develop novel, well-defined, synthetic extracellular matrices for growth and differentiation of hESC. (ca.gov)
Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. (bio-protocol.org)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis. (thermofisher.com)

In addition, we examined whether these substrates will support differentiation of retinal pigmented epithelial cells, an important, clinically relevant cell type that is currently in clinical trials for eye disease. (ca.gov)
Under these conditions, the intestinal cells self-arrange to form a single epithelial layer. (moleculardevices.com)
Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)
After 24-30 hours, mitosis begins to restore epithelial cell population. (medscape.com)
A sufficient supply of progenitor stem cells to facilitate epithelial cell proliferation is important for the cornea. (medscape.com)
A deficiency of limbal stem cells, from either disease (eg, aniridia) or trauma (eg, chemical burn), can preclude adequate epithelial wound healing. (medscape.com)
These processes are facilitated by a phenotypic change among quiescent keratocytes to become active myofibroblasts, a task mediated by transforming growth factor-beta (of presumptive epithelial origin). (medscape.com)
Metalloproteinases are secreted as proenzymes by neutrophils infiltrating the wound, injured epithelial cells, and keratocytes. (medscape.com)

These cells maintain a constant equilibrium between differentiation and self-renewal. (kbvresearch.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)

However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)

An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)

We demonstrate that PLL provides favourable microenvironment for MSC culture by reversing the replicative senescence. (hindawi.com)
Not only are these materials easily obtained, but the natural materials provide attachment sites for cells and a similar microenvironment that provides the cell signaling pathways necessary for their proper differentiation. (wikipedia.org)
Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
Protein-coated cell culture tools provide a three-dimensional microenvironment for optimal adhesion and proliferation of cells. (kbvresearch.com)

These processes generally involve growth factors (GFs), substances that control cell function through the activation of specific signalling pathways and that are capable of stimulating cellular growth, proliferation, and cellular differentiation. (interface-online.org.uk)

ReNcell CX Human Neural Progenitor Cell Line & Culture Media Kit ReNcell CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells. (thomassci.com)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

However, technical challenges persist in the standardization of culture growth conditions and differentiation protocols. (the-scientist.com)
Rapid advancements in this vertical include protocols for directed differentiation, defined culture systems, demonstration of applications in drug screening, the establishment of various disease models, and evaluation of therapeutic potential in treating incurable diseases. (grandviewresearch.com)
Variations in environmental conditions during bioprocessing have the potential to significantly impact the outcome of stem cell cultures, making it difficult to reproduce experimental protocols and interpret the results obtained. (genengnews.com)

For example, after 1 hr exposures to 0.5 μ/ml, surviving fractions determined by colony formation assay were approximately 0.3 for cells from spheroids treated intact and 0.001 for single exponential phase cells. (researchgate.net)
MMC also induced phosphorylation of the nuclear histone marker of DNA damage, γH2AX (a member of the H2A histone family), without induction of cell cycle entry or immediate DNA repair measured by Comet assay. (molvis.org)

The principal aim of this thesis was to investigate the ability of surface topography in inducing bone cell differentiation for biomedical purposes. (gla.ac.uk)
Calcium cross-linked sodium alginate hydrogels have several advantageous features making them potentially suitable as tissue engineering scaffolds and this material has been previously used in many biomedical applications. (ox.ac.uk)

Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
Hit compounds from such screens are not necessarily effective on hypoxic and slowly proliferating cells in 3-D tumour tissue. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)
Growth of tumour and normal cells and senescence. (pmf.hr)

For instance, Science University and Oregon Health are engaged in developing blastocyst staged embryos of around 150 cells in order to provide hESC source. (grandviewresearch.com)
Moreover, the government of Asian countries is supportive of hESC research thus boosting growth in this region. (grandviewresearch.com)
This surface proved to be efficient at supporting growth of undifferentiated hESC as well as differentiation of useful cell types. (ca.gov)
There is a critical need for new technologies to facilitate growth and differentiation of human embryonic stem cells (hESC) using clinically acceptable, animal-free reagents. (ca.gov)
The lead investigators for the center-initiated projects are expert in genomics, hESC and iPSC derivation and differentiation, and bioinformatics. (ca.gov)

The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
In particular, most currently used culture conditions are not acceptable for standardized production of clinical grade cell products. (ca.gov)
Both academic and commercial collaborations are essential in this research, which addresses the complete stem cell process from donor or patient biopsy through to clinical implantation into the patient. (genengnews.com)
This study reports useful information on the limits of the organotypic culture of neonatal mouse testes, which has been regarded as an experimental strategy that can be extended to humans in the clinical setting for the conservation and subsequent re-use of testicular tissue. (elifesciences.org)
rhBMP-2 is a powerful growth factor that is essential in tissue morphogenesis and is utilised to promote bone growth in trauma, spine and maxillofacial clinical applications. (interface-online.org.uk)
There are concerns about the use of growth factors in clinical applications because of their short half-lives, high costs and low effectiveness. (utwente.nl)
These results provide a scientific experimental basis not only for further NSCs clinical transplantation applications, but also for the drug screening and mechanism research on the treatment of diseases of the nervous system. (ox.ac.uk)
Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term growth and functional differentiation. (labroots.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. (thermofisher.com)

Here we have succeeded in inducing human iPSC to recapitulate the main steps of retinal development and to form fully laminated 3D retinal tissue by exploiting the intrinsic cues of the system to guide differentiation ( Supplementary Fig. 1 ). (nature.com)
CEPT improved routine cell passaging, cryopreservation/thawing of pluripotent and differentiated cells, embryoid body and organoid formation, single-cell cloning, genome editing, and new iPSC line generation. (labroots.com)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)

The goal is to characterize the dynamic flow conditions which drive endothelial cell mechanotransduction, cellular growth, and morphological maturation, as well as the differentiation of ADSCs into endothelial-like phenotypes. (aps.org)
The study included assessments of cell growth rate, their morphological features, and their liver detoxification functions. (usda.gov)
The study included assessments of growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible P450 activity, ammonia clearance, and urea production. (usda.gov)

Induced pluripotent stem cells (iPSCs) hold great potential for the modelling of these diseases or as potential therapeutic agents. (nature.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
Pluripotent stem cells, both induced pluripotent stem cells and embryonic stem cells, represent an attractive, physiologically relevant cellular system for disease modeling, drug discovery, and cell-based therapy. (the-scientist.com)
Cellular co-culture was subjected to wall shear stresses from physiological flow forcing for up to 8 hours each day and then followed by steady flow. (aps.org)
Post-facto analysis was performed on dissected vessels using confocal microscopy techniques, live/dead staining, and polymerase chain reaction (PCR) testing to probe cellular growth and proliferation indicators. (aps.org)
These cells are used to create model systems for research, the investigation of cellular structure and function, stem cell research, drug development, and genetic engineering. (kbvresearch.com)
The data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
No differentiation and self-organization into multi-cellular bile ductules, as observed in the parental PICM-19 cell line, occurred within the PICM-19H cell monolayer even after six months of maintenance culture. (usda.gov)
These data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
This delay is required for preparatory cellular changes prior to rapid cell movement. (medscape.com)

The typical seed train cell expansion process involves thawing cryopreserved cells followed by successive expansions into larger culture vessels including flasks, spinners, and stirred bioreactors. (sigmaaldrich.com)
Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)

then is a prospective Download Cell Growth, Differentiation, And Senescence: A Practical Approach to make - their difficult search. (souroujon.com)

In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells. (sigmaaldrich.com)

The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. (sigmaaldrich.com)
Possible applications of 3D cell culture in drug discovery will be discussed, and example of formation of spherical growth of three different human breast cancer cells (MDA-MB-231, SK-BR-3 and T-47D cells) in 3D format will be shown. (srce.hr)
The global Human Embryonic Stem Cells market size was valued at USD 494.7 million in 2015. (grandviewresearch.com)
Particularly, pathological and toxicology modeling can be performed by the provision of physiological models for different human cell types. (grandviewresearch.com)
Variation in the policies across the globe is expected to impact the global human embryonic stem cell market development. (grandviewresearch.com)
Finally, we identified a cyclic RGD peptide that supports growth of human embryonic stem cells. (ca.gov)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
The human endometrial is a dynamic tissue, which undergoes cycles of growth and regression with each menstrual cycle. (scirp.org)
ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. (thomassci.com)
The program directors have significant prior experience managing large-scale federally-funded genomics research programs, and have published many high impact papers on human stem cell genomics. (ca.gov)
Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
Moreover, it has recently been shown that mouse and human ES cells can develop into a three-dimentional (3D) optic cup in culture that remarkably resembles the embryonic vertebrate eye 20 , 21 . (nature.com)
La Jolla, CA - Salk Institute scientists have isolated cells from the brains of human cadavers that can grow, divide and form specialized classes of brain cells. (salk.edu)
The study was done in collaboration with Phillip Schwartz and Stuart Stein at Children's Hospital of Orange County, Calif. The study, titled "Progenitor cells grown from postmortem human brain," was supported by the National Institutes of Health, the National Institute of Aging, the Lookout Fund and the Christopher Reeves Paralysis Foundation. (salk.edu)
editors Culture of human stem cells. (scielo.br)
Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. (utwente.nl)
The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. (bio-protocol.org)
We recently reported the development of a new small molecule cocktail that can be used as a universal end-to-end solution for stress-free culture of human cells. (labroots.com)
Articles The UROtsa cell line was isolated from a primary culture of normal human urothelium through immortalization with a construct containing the SV40 large T antigen. (archive.org)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
Use of human feeders derived under xeno-free conditions can maintain the xeno-free nature of the culture system and allow for the use of low levels of bFGF. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

Collagen I initiates endothelial cell morphogenesis by inducing actin polymerization through suppression of cyclic AMP and protein kinase A." Journal of Biological Chemistry 278, no. 1 (January 2003): 327-334. (thermofisher.com)
The malformations have a normal endothelial cell growth cycle that affects the veins, the capillaries, or the lymphatics, and they do not involute. (medscape.com)

The Scientist brings together a panel of experts to discuss challenges and strategies for optimizing your pluripotent stem cell culture. (the-scientist.com)

Their findings indicate that postmortem tissue may be a potential source of multipotent stem cells, with a variety of uses and applications. (salk.edu)
Sonoyama W, Yamaza T, Gronthos S, Shi S. Multipotent stem cells in dental pulp. (scielo.br)

Cylindrical vessel scaffolds were 3D printed using polylactic acid (PLA) material and the vessel lumen were inoculated with a co-culture of HUVEC and ADSC in a custom-built bioreactor system using cell growth medium ((DMEM, Corning®) and (ECM, Cell Applications Inc)). The vessels were installed in a sterile experimental flow loop facility capable of providing physiological flow forcing conditions. (aps.org)
Neural stem cells (NSCs) obtained from the fetal mouse hippocampus were cultured in the collagen-chitosan composite scaffolds and the biocompatibility was studied. (ox.ac.uk)

Comparison of bone marrow cell growth on 2D and 3D alginate hydrogels. (ox.ac.uk)
It was found that bone marrow cell proliferation was diminished as the concentration of alginate in the 2D hydrogel substrates increased, yet proliferation was extensive on tubular alginate constructs with high alginate contents. (ox.ac.uk)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)

This integrated system allows for automated monitoring, maintenance, and characterization of growth and differentiation of organoids and stem cells, as well as testing the effects of various compounds. (moleculardevices.com)
Cell Applications, Inc. is built on strong foundations of high cell purity, quality control and characterization. (probioscience.org)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. (thomassci.com)
Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells. (thomassci.com)
Such a cell would be a neural stem cell by a strict definition. (salk.edu)

Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)
Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. (thomassci.com)
After each passage, PICM-19H cells grew to a nearly confluent monolayer of cells of hepatocyte morphology, i.e., cuboidal cells with centrally located nuclei joined by biliary canaliculi. (usda.gov)
It proliferates in serumcontaining growth medium as a cell monolayer with little evidence of uroepithelial differentiation. (archive.org)

Projects 1-3 will use multi-level genomics approaches to study stem cell derivation and differentiation in heart, tumors and the nervous system, with implications for understanding disease processes in cancer, diabetes, and cardiac and mental health. (ca.gov)
Breast metastatic tumors in lung can be substituted by lung-derived malignant cells transformed by alternative splicing H19 lncRNA. (medscape.com)

During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
The cells were also successfully tested for their ability to be stably transfected using expression vectors containing the MT-3 or MT-2A genes. (archive.org)

Bioreactors provide controlled microenvironments and nutrient delivery to regulate cell growth and differentiation, improving standardization and reproducibility. (sigmaaldrich.com)

The second application of the system is for 'brain training' in spinal cord injured patients for relief of chronic nerve pain. (interface-online.org.uk)

There is high potential for manual operations to impact the outcomes of stem cell cultures, where parameters such as temperature and pH cannot be controlled. (genengnews.com)

cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Because cell lines are unique and require different nutrients to thrive, culture biomass and protein production are largely dependent on the culture media used. (sigmaaldrich.com)
It provides a straightforward way to scale up culture systems for industrial production of cell or protein-based therapies, or for research purposes. (wikipedia.org)
Protein expression profiles indicated that ERK1/2 might play part in cell proliferation and cell differentiation. (gla.ac.uk)
The Global Cell Culture Protein Surface Coating Market size is expected to reach $1.9 billion by 2028, rising at a market growth of 15.5% CAGR during the forecast period. (kbvresearch.com)
However, the cell culture protein surface coating market was positively impacted due to the outbreak. (kbvresearch.com)
The cell culture protein surface coating method is a very efficient and advanced approach in the healthcare and life science sector. (kbvresearch.com)
Based on Coating Type, the Cell Culture Protein Surface Coating Market is bifurcated into Self-coating and Precoating. (kbvresearch.com)
In 2021, the precoating segment garnered a significant revenue share of the cell culture protein surface coating market. (kbvresearch.com)
MMC significantly blocked TGFβ-induced cell proliferation and myofibroblast differentiation in cultured quiescent keratocytes and altered the transcriptional regulation of macrophage chemotactic protein-1 (MCP-1) and alpha smooth muscle actin (αSMA). (molvis.org)
Estrogens are important regulators of growth and differentiation in (range, 0 -9 fmol/mg protein, median 0.7). (lu.se)
Vinculin, a plasma protein, links fibers to talin, which is a cell membrane protein. (medscape.com)

Anatomy64

Organisms8

Diseases8

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes58

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science6

Health Care2

Vitro32

Proliferation and differentiation3

Mesenchymal10

Vivo6

Biology14

Tissues9

Serum-free6

Regenerative medicine7

Fibroblast10

Osteogenic2

Differentiate11

Proteins12

Stromal14

Neurons7

Regeneration4

Methods18

Lineages2

Assays4

Extracellular6

Epithelial8

Self-renewal2

Terminal differentiation1

Induce differentiation1

Microenvironment4

Pathways1

Progenitor cell2

Protocols3

Assay2

Biomedical2

Tumour4

HESC5

Clinical9

Multilineage1

IPSC3

Induction1

Morphological3

Therapeutic2

Cellular10

Expansion2

Senescence1

Monoclonal antibody1

Human29

Endothelial cell2

Pluripotent stem cell cu1

Multipotent stem2

Scaffolds2

Bone marrow3

Characterization3

Fetal bovin1

Neural3

Monolayer6

Tumors2

Genes2

Standardization1

Spinal cord1

Stem cell cultures1

Morphology1

Protein11