In flow cytometry, the average fluorescence intensity for rabbit BMSCs incubated with FITC-DPI was significantly higher than that of FITC-LSP ( P = 2.733 × 10 −8 ). (biomedcentral.com)
Assays3
The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. (researchsquare.com)
The high interest in improved diagnostic, prognostic and therapy control procedures in medicine as well as cellular aspects in immunology, cancer research, molecular biology, biotechnology and microbiology is concurrent with the development of cytometric assays for a variety of biochemically specific cell functions and markers, of instrumentation for flow and image cytometry and of software for cytometric multiparameter data processing. (purdue.edu)
These tools would overcome challenges with widely used methods that are destructive (e.g., histology, flow cytometry) or lack cellular resolution (e.g., plate-based assays, whole animal bioluminescence imaging). (bvsalud.org)
Bone marrow4
In the present study, we sought to investigate whether bone marrow evaluation provides more accurate diagnosis in patients with HVA. (biomedcentral.com)
We evaluated characteristics of the bone marrow mast cells by pathology, flow cytometry and detection of D816V mutation by using current WHO-criteria, which led to changes in the final diagnosis compared to the assessments done by classical allergy work-up and measurements of sBT. (biomedcentral.com)
Bone marrow samples from C57BL/B6 mice and one healthy donor were analyzed using traditional two-marker (CD71 and glycophorin A) flow cytometry analysis. (biomedcentral.com)
With the help of AQP0 staining, we could define up to five stages of erythropoiesis in both mouse and human bone marrow using flow cytometry. (biomedcentral.com)
Detection5
The detection of cell populations in flow cytometry data has long been dependent on "manual gating" by visual inspection. (biomedcentral.com)
Modern flow cytometers allow the simultaneous detection of nearly 20 protein markers per cell with a throughput of thousands of cells per second. (biomedcentral.com)
This innovative diagnostic technique has the potential to transform cancer diagnosis by enabling the development of non-invasive or minimally invasive methods for detection, prognosis, and monitoring of cancer patients. (ethz.ch)
Bismuth iodide (BiI 3 ), lead iodide (PbI 2 ) and mercuric iodide (HgI 2 ) have theoretical gamma-ray detection efficiencies approximately 2-3 times higher than CdZnTe, the current compound semiconductor material proposed for use in several homeland/national security applications, over the range of 200-3000 keV. (spie.org)
Genomics research provides a new feasible solution for the detection, diagnosis, target selection and prognostication of BC. (biomedcentral.com)
Cellular5
Flow cytometry is a widely used technology for identifying and quantifying cellular properties and cell populations by measuring expression levels of surface and intracellular proteins at the single-cell level. (biomedcentral.com)
Aim: This perspective aims to (1) justify the need for label-free microscopy to track heterogeneous cellular functions over time and space within unperturbed systems and (2) recommend improvements regarding instrumentation, image analysis, and image interpretation to address these needs. (bvsalud.org)
Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)
Peripheral blood cellular immunological changes were assessed by flow cytometry and transcript levels of BAFF , interferon (IFN)-induced and plasma cell-expressed genes were quantified by NanoString. (biomedcentral.com)
Intravital imaging of pathophysiological cellular dynamics in natural in vivo microenvironment in various preclinical animal model can provide an unprecedented insight in the dynamic processes of human diseases beyond conventional histological observation of ex vivo sample or in vitro culture sample. (accela.eu)
The Maria Sklodowska-Curie Memorial Institute Tumor Pathology Department, Gliwice ranks high as one of the most modern and best equipped pathology departments in Europe thanks to the steady implementation of current knowledge and techniques in the examination process. (gov.pl)
roliferating activity of tumor cells and DNA ploidy using flow or static cytometry. (gov.pl)
To obtain detailed systematic tumor immune profile, we performed an in-depth immunoassay on 12 human urothelial carcinoma tissue samples and 14 paratumor tissue samples using mass cytometry. (biomedcentral.com)
The IMSR works closely with the Flow Cytometry Core, the Integrated Genomic Shared Resources, and the Tumor Tissue and Serum Repository at the Georgia Cancer Center. (augusta.edu)
We treated osteosarcoma tumor-burdened mice by using needling on different acupoints and acupoints combination, thereafter determined the effects of acupuncture on tumor growth by using imaging technology in vitro. (biomedcentral.com)
Assay2
The expression levels of PD-1 and sPD-L1 were measured using flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. (bvsalud.org)
Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. (bvsalud.org)
Differential3
A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. (elifesciences.org)
We have a wide range of antibodies which we continuously enlarge so as to guarantee our patients the most precise differential diagnosis. (gov.pl)
Based on the differential expression levels of cell-surface molecules during erythropoiesis, flow cytometry analysis is a useful tool for analyzing the erythropoiesis process [ 8 ]. (biomedcentral.com)
Early diagnosis4
Your overall outlook depends on early diagnosis. (healthline.com)
Consequently, a better understanding of the mechanism underlying ESCC formation and progression is required for improving early diagnosis and therapy. (researchsquare.com)
There is a critical need for better understanding of IA pathogenesis in order to provide improved early diagnosis and pharmacological treatment alternatives to patients who are at high risk for surgical interventions. (biomedcentral.com)
Its lethality is a consequence of the lack of symptoms or biomarkers enabling the early diagnosis of disease, and the propensity of malignant cells to seed the peritoneal cavity. (biomedcentral.com)
Clinical1
Diagnosis of systemic autoimmune rheumatic diseases (SARD) relies on the presence of hallmark anti-nuclear antibodies (ANA), many of which can be detected years before clinical manifestations. (biomedcentral.com)
Feasible1
In addition to light microscopy, chromosome analysis, and polymerase chain reaction, flow cytometry is a feasible and fast method for quantitatively analyzing hematological diseases. (biomedcentral.com)
Instrumentation2
Our instrumentation affords the ability to perform almost every published flow cytometry protocol. (augusta.edu)
Conclusion: Improvements in instrumentation including strategies that increase resolution and imaging speed, standardization and centralization of image analysis tools, and robust data validation and interpretation will expand the applications of label-free microscopy to study heterogeneous and dynamic biological systems. (bvsalud.org)
Analyze3
This helps them analyze your symptoms, which provides a baseline for your diagnosis. (healthline.com)
However, such research is currently limited by a lack of a systematic mathematical framework to adequately model and analyze time-series flow cytometry data. (biomedcentral.com)
Disease6
Although each test helps to narrow down the final diagnosis they become increasingly expensive and time-consuming to perform, and rapid action is often important when treating a disease. (elifesciences.org)
Ongoing work to elucidate the mechanisms behind IA pathogenesis promises to provide new targets for the diagnosis and treatment of this disease. (biomedcentral.com)
Current understanding characterizes IA as a chronic inflammatory disease. (biomedcentral.com)
The faculty and staff of the Georgia Esoteric and Molecular (GEM) Core provide diagnostic services for the diagnosis and comprehensive evaluation of disease primarily through the molecular analysis of tissues and cells. (augusta.edu)
Purdue E-mail box) ( 3.2.3 ), by advancing scientific software development ( 3.2.7 ) for the standardized establishment of individual patient disease diagnosis and especially disease prognosis from multiparameter cytometric and other data and by promoting international quality assurance ( 3.2.2 ) programs and educational offers (STEP program, telelearning ( 3.2.6 ) in this area. (purdue.edu)
However, this did not improve the outcome in patients harboring macrometastases at diagnosis or those with relapsed disease [ 1 , 10 ]. (ijbs.com)
Therapies2
Current targeted therapies for BC mainly include targeted endocrine therapy for hormone receptor-positive BC and epidermal growth factor receptor (HER)-2-positive BC. (biomedcentral.com)
As research provides evidence and expands our understanding of carcinogenic mechanisms, studies on intestinal flora present many more opportunities to develop therapies for cancer diagnosis and management. (biomedcentral.com)
Widely1
Modern flow cytometry technology has enabled the simultaneous analysis of multiple cell markers at the single-cell level, and it is widely used in a broad field of research. (biomedcentral.com)
Markers1
However, because sufficient specific cell markers are scarce, dyserythropoietic diseases are challenging to identify through flow cytometry. (biomedcentral.com)
Analysis4
Time-series flow cytometry data are indispensable for investigating the dynamics of cell populations that could not be elucidated by static time-point analysis. (biomedcentral.com)
An important challenge in the analysis of flow cytometry data is the classification of individual cells into canonical cell types, that is, subset populations such as T and B cells. (biomedcentral.com)
Flow cytometric analysis demonstrated the high purity of generated microsamples. (researchgate.net)
Significance: Advancements in label-free microscopy could provide real-time, non-invasive imaging with unique sources of contrast and automated standardized analysis to characterize heterogeneous and dynamic biological processes. (bvsalud.org)
Biology1
For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology, however, requires that (1) appropriate process controls are collected along with experimental samples, (2) these process controls are used for unit calibration and quality control, and (3) data are analyzed using appropriate statistics. (bvsalud.org)
Microscopy1
Based on the strengths (e.g., multiple sources of molecular contrast, non-invasive monitoring) and weaknesses (e.g., imaging depth, image interpretation) of several label-free microscopy modalities, improvements for future imaging systems are recommended. (bvsalud.org)
Cell5
We propose a simple and efficient statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), to perform clustering and cell population tracking for time-series flow cytometry data. (biomedcentral.com)
Our results indicate that CYBERTRACK offers better understandings of time-dependent cell population dynamics to cytometry users by systematically analyzing time-series flow cytometry data. (biomedcentral.com)
Time-series flow cytometry data offer information on longitudinal cell population dynamics that could not be elucidated by conventional static time-point data. (biomedcentral.com)
To address this problem, we propose a new statistical framework, named CYBERTRACK (CYtometry-Based Estimation and Reasoning for TRACKing cell populations), for the automatic clustering and tracking of a mixture proportion of cell populations in time-series flow cytometry data. (biomedcentral.com)
CYBERTRACK assumes that flow cytometry data are generated from a multivariate Gaussian mixture distribution with its mixture proportion at the current time dependent on that at a previous timepoint. (biomedcentral.com)
Patients1
The current study was performed to examine the feasibility, safety and immunogenicity of adding P10s-PADRE to standard-of-care chemotherapy in HR+/HER2− early-stage breast cancer patients. (oncotarget.com)
Dynamics1
The CYBERTRACK performance is validated using two real flow cytometry datasets, which demonstrate that the population dynamics detected by CYBERTRACK are consistent with our prior knowledge of lymphocyte behavior. (biomedcentral.com)
Fast2
This method forces the blood cells in a small drop of blood to flow extremely rapidly through a narrow microfluidic channel while they are imaged by a fast camera. (elifesciences.org)
Cytometry is a fast developing multidisciplinary science. (purdue.edu)
Diagnostic2
Diagnosis is a key step in the treatment process, and improving diagnostic technology has shown outsized positive effects in personal health and population health (a lesson underscored by the COVID-19 pandemic). (ethz.ch)
Resource-poor countries and regions require effective, low-cost diagnostic devices for accurate identification and diagnosis of health conditions. (mdpi.com)
Cells2
But there might be several reasons why the number of white blood cells has increased, so this information alone is often not enough for a specific diagnosis. (elifesciences.org)
Flow cytometry showed that much more cells were arrested in G2/M phasein Stathmin downregulated group, compared with the non-transfection group and NC group. (biomedcentral.com)
Diseases1
However, since high levels can occur due to other diseases, you'll need other tests to help with the diagnosis. (healthline.com)
Functional1
What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. (elifesciences.org)
Technique1
now show that this is possible using a technique called real-time deformability cytometry. (elifesciences.org)
Tests3
They can provide a diagnosis using tests like blood panels, biopsies, and more. (healthline.com)
A doctor will carry out several tests to confirm a non-Hodgkin's lymphoma diagnosis. (healthline.com)
A lymphoma diagnosis often includes imaging tests. (healthline.com)
Blood2
Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. (researchgate.net)
The Flow Cytometry Core Facility is a shared resource providing access to flow cytometers, support equipment, and associated software and services. (augusta.edu)
Evaluation1
Cytologic material undergoes complex evaluation applying immunohistochemistry flow cytometry and digital image analyses. (gov.pl)