Deoxyribonuclease IV (phage-T4-induced) (EC 3.1.21.2, endodeoxyribonuclease IV (phage T4-induced), E. coli endonuclease IV, endodeoxyribonuclease, redoxyendonuclease, deoxriboendonuclease, Escherichia coli endonuclease II, endonuclease II, DNA-adenine-transferase) is catalyzes the degradation nucleotides in DsDNA by attacking the 5'-terminal end. (wikipedia.org)
In addition, the endonuclease IV consists of several activities such as AP endonuclease, 3'-diesterase, 3'->5' exonuclease, and 3'phosphatase. (wikipedia.org)
The endonuclease IV falls under a structurally resembling members with apyrimidininc endonuclease I (APE1). (wikipedia.org)
DNase IV prefers to attack native DNA acting as an endonuclease with metal ions either Mg++ or Mn++. (wikipedia.org)
Enzymes recognizing helical parameters such as groove width and flexibility(Dnase-I) or helical distortions caused by abasic sites (exonuclease III,HAP1), and enzymes specific for such structures as flap DNA (5′nucleases of eukaryotes and phage and eubacterial DNA polymerases) and four-way junctions (T4 endonuclease VII, RuvC) have been reported (reviewed by Suck, 1997 ). (silverchair.com)
Mammalian6
Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coliDNA polymerase I". The Journal of Biological Chemistry. (wikipedia.org)
Little is known concerning lambda phage-mediated gene transfer and expression in mammalian hosts. (biomedcentral.com)
For this purpose, we constructed recombinant λ-phage nanobioparticles containing a mammalian expression cassette encoding enhanced green fluorescent protein (EGFP) and E7 gene of human papillomavirus type 16 (λ-HPV-16 E7) using Lambda ZAP- CMV XR vector. (biomedcentral.com)
The results demonstrated that the recombinant λ-phages, due to their capabilities in transducing mammalian cells, can also be considered in design and construction of novel and safe phage-based nanomedicines. (biomedcentral.com)
The dimensions of the lambda phage particles are broadly similar to those of many mammalian viruses and recent structural evidence points to a shared ancestry between tailed bacteriophages and mammalian DNA viruses [ 25 ]. (biomedcentral.com)
Since little is known concerning phage-mediated gene transfer in mammalian cells [ 26 ], we therefore performed experiments to examine phage-mediated gene transfer in vitro . (biomedcentral.com)
Enzymes2
The activities of DNase III and DNase IV depend on two Mg++ as cofactors and these enzymes are localized in cell nuclei. (wikipedia.org)
DNase IV acts on double stranded DNA in repair by breaking phosphodiester bonds, but the number of cleavages by this enzyme is smaller than the extent of polymerization of DNA. (wikipedia.org)
Cytoplasm2
70% of the total DNase IV activitiy was found in the cytoplasm while 30% was found in cell nuclei. (wikipedia.org)
After macropinocytosis and internalization, phage must gain access to the cytoplasm, uncoat and deliver its DNA payload to the nuclei. (biomedcentral.com)
Protein1
The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)
Group1
Mice vaccinated with λ-HPV-16 E7 are able to generate potent therapeutic antitumor effects against challenge with E7- expressing tumor cell line, TC-1 compared to group treated with the wild phage. (biomedcentral.com)
Specific1
DNase IV does not recognize specific sequences on DNA for non-staggered cleavage. (wikipedia.org)
Major2
In crude cell extracts from lymphoid organs, DNase III and DNase IV show major activities because DNase I activity is inhibited. (wikipedia.org)
This graph shows the total number of publications written about "Deoxyribonuclease I" by people in this website by year, and whether "Deoxyribonuclease I" was a major or minor topic of these publications. (umassmed.edu)
Activity1
Deoxyribonuclease IV is a type of deoxyribonuclease that has both an exonucleolytic and an endonucleolytic activity. (wikipedia.org)
Multiple1
DNase IV works by attacking multiple polynucleotide chains at the same time. (wikipedia.org)
Cell2
Four cell lines (COS-7, CHO, TC-1 and HEK-239) were transduced with the nanobioparticles. (biomedcentral.com)
For this purpose, recombinant lambda phages were characterized to compare their performance for gene delivery and expression in four cell lines from different sources. (biomedcentral.com)
Human1
In human body, DNase IV was required for cleavage of a reaction intermediate generated by template strand displacement during gap-filling. (wikipedia.org)
Escherichia4
In this study, we have explored the biological effects of NO(.)-induced DNA damage on Escherichia coli. (nih.gov)
Deoxyribonuclease IV (phage-T4-induced) (EC 3.1.21.2, endodeoxyribonuclease IV (phage T4-induced), E. coli endonuclease IV, endodeoxyribonuclease, redoxyendonuclease, deoxriboendonuclease, Escherichia coli endonuclease II, endonuclease II, DNA-adenine-transferase) is catalyzes the degradation nucleotides in DsDNA by attacking the 5'-terminal end. (wikipedia.org)
Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coliDNA polymerase I". The Journal of Biological Chemistry. (wikipedia.org)
PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE INDUCED BY INFECTION WITH PHAGE 7T4+ * (Received for publication, August 25, 1967) Merunran Gouiian,{ Zottan J. Lucas,§ anp ArtHuR KornBere From the Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California 94304 SUMMARY DNA polymerase induced in Escherichia coli by phage T4 am N82 has been prepared in a highly purified state. (nih.gov)
Coli4
The evidence indicates that the phage polymerase, unlike the E. coli enzyme, is unable to initiate new strands or utilize a fully helical DNA as template. (nih.gov)
It infects E. coli and is the best known of the T-even phages. (lookformedical.com)
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. (lookformedical.com)
A series of 7 virulent phages which infect E. coli. (lookformedical.com)
Enzyme5
DNase IV acts on double stranded DNA in repair by breaking phosphodiester bonds, but the number of cleavages by this enzyme is smaller than the extent of polymerization of DNA. (wikipedia.org)
The purified phage enzyme contains an exonuclease activity which is physically inseparable from the polymerase activity but is suppressed or obscured by conditions favorable for replication. (nih.gov)
Recent work with conditional lethal mutants has justified the earlier pre- sumptions that this enzyme, unique to phage infection, is the product of a phage gene and is essential for synthesis of phage DNA (5, 6). (nih.gov)
In the present study, we have examined the properties which distinguish the phage-induced polymerase from the host enzyme: the requirement for a single stranded template and the limited extent of replication. (nih.gov)
A procedure is presented for preparation of highly purified phage T4 polymerase, followed by a description of the physical and enzymatic properties of the enzyme. (nih.gov)
Residues1
DNase IV is composed of 185 amino acid residues with magnesium ions acting as a cofactor. (wikipedia.org)
Infection3
The increase in rate of deoxyribonucleic acid synthesis as- sociated with infection by virulent coliphages (1) was shown, in the case of phages T2, T4, and T5, to be accompanied by the * Supported in part by grants from the National Institutes of Health, United States Public Health Service, and the National Science Foundation. (nih.gov)
BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. (lookformedical.com)
All cultures from nine seronegative adult and 12 foetal donors gave rise to cell lines following subculture 4 weeks post infection. (nih.gov)
Replication1
Such studies, we anticipate, will extend our understanding of DNA synthesis in general and phage DNA replication in particular. (nih.gov)
Virulent2
Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium. (lookformedical.com)
Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. (lookformedical.com)
Descriptor1
Deoxyribonuclease I" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). (lookformedical.com)
A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6. (lookformedical.com)
Excision repair1
The relative importance of base excision repair, nucleotide excision repair (NER), and recombinational repair in preventing NO(.)-induced toxicity was determined. (nih.gov)
Bacterial1
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. (lookformedical.com)
Double-strand2
One consequence of these double-strand ends is that NO(.) induces homologous recombination at a genetically engineered substrate. (nih.gov)
DNase III cleaves a single strand of DNA from 3' terminal end but DNase IV cleaves a double strand of DNA from 5' terminal end. (wikipedia.org)
Type1
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. (lookformedical.com)
Reaction2
In human body, DNase IV was required for cleavage of a reaction intermediate generated by template strand displacement during gap-filling. (wikipedia.org)
PMID- 214394 TI - Mechanism of aromatic hydroxylation : the kinetic mechanism & the involvement of superoxide anions in the reaction catalyzed by m-hydroxybenzoate-4-hydroxylase from Aspergillus niger (UBC-814). (nih.gov)
Ends1
DNase IV attacks dsDNA at 5' ends by liberating 5' mononucleotides but it does not attack any monomers in polydeoxyribonucleotides in a random fashion. (wikipedia.org)
Study1
In this study we demonstrate that, after intramuscular injection in chickens, it induces hepatocellular carcinomas of the liver and adenocarcinomas of the kidney. (nih.gov)