Protein DenaturationAmino Acid SequenceProtein ConformationProteinsModels, MolecularProtein Structure, SecondaryAmino AcidsSequence Homology, Amino AcidMolecular Sequence DataProtein Structure, TertiarySequence Analysis, ProteinProtein FoldingSequence AlignmentDatabases, ProteinNucleic Acid DenaturationStructural Homology, ProteinBase SequenceCloning, MolecularSoftwareBinding SitesAlgorithmsAmino Acid SubstitutionComputational BiologyCircular DichroismThermodynamicsEscherichia coliProtein BindingModels, ChemicalKineticsStructure-Activity RelationshipGuanidineMutationHot TemperatureComputer SimulationAmino Acid MotifsCrystallography, X-RayTemperatureRecombinant ProteinsMutagenesis, Site-DirectedInternetDNAPeptide FragmentsSequence Homology, Nucleic AcidBacterial ProteinsPeptidesEnzyme StabilityConserved SequenceNuclear Magnetic Resonance, BiomolecularEvolution, MolecularUreaSubstrate SpecificityNucleic Acid ConformationHydrogen BondingMolecular WeightCalorimetry, Differential ScanningElectrophoresis, Polyacrylamide GelDNA, ComplementaryCattlePhylogenyHydrogen-Ion ConcentrationSpectrometry, FluorescenceAmino Acids, EssentialUser-Computer InterfaceMagnetic Resonance SpectroscopyMembrane ProteinsHydrophobic and Hydrophilic InteractionsAmino Acid Transport SystemsTrypsinSequence Analysis, DNASolventsMolecular StructureCysteineProtein Structure, QuaternaryRestriction MappingCell LineRecombinant Fusion ProteinsSpecies SpecificityAmino Acids, AromaticGuanidinesPlasmidsMuramidaseCarrier ProteinsDisulfidesMacromolecular SubstancesProtein StabilityLigandsComputer GraphicsRNA, MessengerGenes, BacterialChromatography, High Pressure LiquidTryptophanDNA PrimersSequence AnalysisSaccharomyces cerevisiaeMolecular Dynamics SimulationProtein EngineeringGenesCatalysisChromatography, GelEscherichia coli Proteins