• Transfection of HepG2 cells with sense or antisense miR-370 or miR-122 upregulated and downregulated, respectively, the transcription factor sterol-regulatory element binding protein 1c (SREBP-1c) and the enzymes diacylglycerol acyltransferase-2 (DGAT2), fatty acid synthase (FAS), and acyl-CoA carboxylase 1 (ACC1) that regulate fatty acid and triglyceride biosynthesis. (nih.gov)
  • PF-06424439 is an oral, potent and selective imidazopyridine diacylglycerol acyltransferase 2 (DGAT2) inhibitor with an IC50 of 14 nM. (adooq.com)
  • Inhibition of triacylglycerol (TAG) synthesis via diacylglycerol acyltransferase 2 (DGAT2) antisense oligonucleotides improves liver steatosis (Yamaguchi et al. (webmedcentral.com)
  • Two DGAT isozymes are encoded by the genes DGAT1 and DGAT2. (wikipedia.org)
  • Mice with genetic disruption of the DGAT1 or DGAT2 genes have been made by the Farese laboratory at UCSF. (wikipedia.org)
  • To test this hypothesis, we selectively deleted both TG-synthesis enzymes, DGAT1 and DGAT2, in adipocytes (ADGAT DKO mice). (elifesciences.org)
  • We generated mice lacking both known TG synthesis enzymes, DGAT1 and DGAT2 1 , 2 , in adipocytes. (elifesciences.org)
  • To generate mice lacking TGs in adipose tissue (ADGAT DKO), we crossed adipose tissue-specific Dgat1 knockout mice (Cre-transgene expressed under control of the mouse adiponectin promoter 3 ) with Dgat2 flox mice 4 . (elifesciences.org)
  • In higher plants and microalgae, there are four major groups of DGATs: (1) the membrane bound form of DGAT1, (2) the membrane bound form of DGAT2 sharing low sequence similarity with DGAT1, (3) the soluble type of DGAT3, which is localized in the cytosol, and (4) the dual function of WS/DGAT, which possesses both wax ester and TAG biosynthesis activities [12-18]. (researchsquare.com)
  • DGAT activity resides mainly in two distinct membrane bound polypeptides, known as DGAT1 and DGAT2. (medchemexpress.cn)
  • ACC inhibitor alone or co-administered with a DGAT2 inhibitor in patients with non-alcoholic fatty liver disease: two parallel, placebo-controlled, randomized phase 2a trials. (nih.gov)
  • Our data suggest that miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha and, subsequently, the expression of other genes that affect lipid metabolism. (nih.gov)
  • Previous studies have identified four putative DGAT2-encoding genes in H. lacustris , and only HpDGAT2D allowed the recovery of TAG biosynthesis, but the engineering potential of HpDGAT2s in TAG biosynthesis remains ambiguous. (researchsquare.com)
  • Five putative DGAT2 genes ( HpDGAT2A , HpDGAT2B , HpDGAT2C , HpDGAT2D , and HpDGAT2E ) were identified in H. lacustris . (researchsquare.com)
  • There was no difference in citrate synthase activity, in the expression of lipogenic proteins (FAS and DGAT2) and FGF21. (usp.br)
  • DGAT2 inhibition is proposed as a mechanism to reduce hepatic triglyceride (TG) accumulation that underlies hepatic injury in non-alcoholic fatty liver disease. (guidetopharmacology.org)
  • LDHB and DGAT2) mainly participated in lipid storage and metabolism. (sruc.ac.uk)
  • In contrast, DGAT2−/− mice have reduced triglyceride levels but are lipopenic, suffer from skin barrier abnormalities (including the inability to retain moisture), and die shortly after birth. (wikipedia.org)
  • We examined the effect of extended-release niacin on liver fat content in Chinese patients with dyslipidemia and whether the common diacylglycerol acyltransferase-2 (DGAT2) polymorphisms influenced this effect. (nih.gov)
  • 3. Diacylglycerol acyltransferase-2 (DGAT2) and monoacylglycerol acyltransferase-2 (MGAT2) interact to promote triacylglycerol synthesis. (nih.gov)
  • 7. Concerted elevation of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin. (nih.gov)
  • 10. Murine diacylglycerol acyltransferase-2 (DGAT2) can catalyze triacylglycerol synthesis and promote lipid droplet formation independent of its localization to the endoplasmic reticulum. (nih.gov)
  • Here we show that two tung (Vernicia fordii) membrane-bound glycerol-3-phosphate acyltransferase (GPAT) enzymes, GPAT8 and a newly-identified putative GPAT with homology to mammalian GPAT3, GPAT9, localize in tobacco BY-2 cells to the same ER subdomain that contains tung type 2 diacylglycerol acyltransferase (DGAT2), but is distinct from the ER subdomains containing DGAT1 or ER export sites. (usda.gov)
  • 1998, 95:13018-13023] and DGAT2 (acyl coA-diacylglycerol acyl transferase 2) [Cases et al. (justia.com)
  • Mutation screen and association studies in the diacylglycerol O-acyltransferase homolog 2 gene (DGAT2), a positional candidate gene for early onset obesity on chromosome 11q13. (cdc.gov)
  • Moreover, the expression levels of diacylglycerol O-acyltransferase (DGAT)-1 and DGAT2, both of which encode enzymes catalyzing the synthesis of triglycerides, were not suppressed by RSG. (biomedcentral.com)
  • Specific inhibition of both diacylglycerol acyltransferase (DGAT) 1 and 2 decreased fatty acid (FA) uptake by myotubes, whereas only DGAT2 inhibition also decreased fatty acid oxidation. (bvsalud.org)
  • Loss of PKR also perturbs major lipid signalling pathways, resulting in augmented expression of fatty acid synthase (FASN) and diacylglycerol transferase 2 (DGAT2), both of which induce triglyceride synthesis and likely contribute to adipose tissue expansion. (monash.edu)
  • Two DGAT isozymes are encoded by the genes DGAT1 and DGAT2. (wikipedia.org)
  • 5. Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat. (nih.gov)
  • DGAT1 and DGAT2 do not share significant protein sequence homology. (justia.com)
  • The roles of DGAT1 and DGAT2 in lipid metabolism and insulin responsiveness of human skeletal muscle were studied using cryosections and myotubes prepared from muscle biopsies from control, athlete, and impaired glucose regulation (IGR) cohorts of men. (bvsalud.org)
  • The differential effects of DGAT1 and DGAT2 inhibition suggest that the specialized role of DGAT2 in esterifying nascent diacylglycerols and de novo synthesized FA is associated with synthesis of a pool of triacylglycerol, which upon hydrolysis results in effectors that promote mitochondrial fatty acid oxidation but decrease insulin signaling in skeletal muscle cells. (bvsalud.org)
  • The oil palm genome contains respectively three, two, two and two distinctly expressed functional copies of the DGAT1, DGAT2, DGAT3 and WS/DGAT genes. (afpm.org.my)
  • Comparison of the predicted DGAT protein sequences with other animal and plant DGATs was consistent with the E. guineensis DGAT1 being ER located with its active site facing the lumen while DGAT2, although also ER located, had a predicted cytosol-facing active site. (afpm.org.my)
  • The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. (biomedcentral.com)
  • An increase in the extent of substitution of SP with TMP inhibited the gene expression of hepatic fructolysis/gluconeogenesis ( KHK , ALDOB , DLD , and FBP1 ), lipogenesis ( FAS , SCD1 , CD36 , and DGAT2 ), and its transcriptional factors ( PPARγ and ChREBP ). (pnfs.or.kr)
  • In contrast, DGAT2−/− mice have reduced triglyceride levels but are lipopenic, suffer from skin barrier abnormalities (including the inability to retain moisture), and die shortly after birth. (wikipedia.org)
  • Winter A. et al "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals" Cytogenet Genome Res. (wellnessadvocate.com)
  • The well-characterized DGATs in higher plants include type-I (DGAT1) and type-II (DGAT2), the two structurally distinct groups of membrane-bound acyltransferases [ 14 ], and type-III (DGAT3), which is soluble and present in the cytosol [ 15 ]. (biomedcentral.com)