CatalaseP450Horseradish peroxidaseHemeProteinEnzymesHydrogen peroxidePseudomonasProteinsAscorbateGenesEnzymeMitochondriaCompoundH2O2ActivityPorphyrinPeroxidesLiverPropertiesActivationEnzymesYeast cytochrome c peroxidaCatalasesHorseradishLigninHydrogen peroxide to waterOxidativeIntracellularElectronMitochondrialProteinProximalTyrosineCaspaseLigandSpeciesStructuralMembraneMutantsReactionPlantCellsClassDescription
Catalase10
- In addition to its peroxidase activity, it acts as a sensor and a signaling molecule to exogenous H2O2, which activates mitochondrial catalase activity. (wikipedia.org)
- Ascorbate peroxidase (APX), cytochrome c peroxidase (CcP), and the catalase - peroxidases (KatG) share very similar active site structures and are distinguished from other peroxidases by the presence of a distal tryptophan residue. (bris.ac.uk)
- M. Kathiresan, D.Martins and A.M. English ( 2014 ) Respiration triggers heme transfer from cytochrome c peroxidase to catalase in yeast mitochondria , Proc. (concordia.ca)
- In the human cell, antioxidant molecules include both nonenzymatic compounds such as glutathione (GSH), thioredoxin (Trx), ascorbate, α -tocopherol, β -carotene, and coenzyme Q10 and enzymes such as catalase, glutathione peroxidase, and superoxide dismutase (SOD) [ 8 ]. (hindawi.com)
- Ozone also causes an increase in the NADH reducing process and helps to oxidize cytochrome C. There is a stimulation of the production of the enzymes which act as free radical scavengers and cell wall protectors: glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase. (silvermedicine.org)
- A series of seven hemeproteins, cytochromes P-450LM2, P-450LM4, and P-420LM2, horseradish peroxidase, chloroperoxidase, catalase, and metmyoglobin, as well as hemin were tested for their ability to catalyze a set of five oxidative reactions. (lookformedical.com)
- All hemeproteins were able to carry out peroxidation, but three (horseradish peroxidase, chloroperoxidase, and catalase) were much better catalysts than the others. (lookformedical.com)
- Molecular docking analysis for different enzymes involved in aging (collagenase, elastase and hyaluronidase), antioxidant enzymes (superoxide dismutase, glutathione-s-transferase, glutathione peroxidase and catalase) and mitochondrial enzymes (nicotinamide adenine dinucleotide (NAD)+hydrogen (H) dehydrogenase, succinate dehydrogenase, cytochrome c oxidase and adenosine triphosphate synthase) was carried out for epigallocatechin gallate alone (1), withaferin A alone (2), epigallocatechin gallate and withaferin A in combination (3) and a reference molecule. (ijpsonline.com)
- Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) activities and uric acid (UA) levels were measured in plasma and saliva. (bvsalud.org)
- To counteract the oxidative stress mammalian cells have antioxidant system which includes enzymatic activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), and non enzymatic antioxidants including vitamins A, E, C, melatonin, uric acid (UA) and glutathione 6,10 . (bvsalud.org)
P4504
- NADPH-cytochrome P450 reductase (CPR) and cytochrome- b 5 ( b 5 ) together with NADH- b 5 reductase ( b 5 R) play important roles in cytochrome P450 3A-mediated drug metabolism via electron transfer. (aspetjournals.org)
- and Galanthus elwesii we have identified a para-para' C-C phenol coupling cytochrome P450, CYP96T1, capable of forming the products (10b R ,4a S )-noroxomaritidine and (10b S ,4a R )-noroxomaritidine from 4′- O -methylnorbelladine. (frontiersin.org)
- Liver microsomes from four rats of each sex per group were assayed for protein and cytochrome P450 content, and the activities of UDP-glucuronyltransferase and several monooxygenases were determined (Vermeir, 1996). (inchem.org)
- Phase I reactions are reduction-oxidation (redox) reactions carried out via the cytochrome P450 enzyme system. (hindawi.com)
Horseradish peroxidase2
- Plant peroxidase such as horseradish peroxidase and pineapple peroxidase B have low lysine, tryptophan, and tyrosine contents and high cysteine content. (wikipedia.org)
- This study reports on the metabolic activation of BHT by two peroxidase enzymes: horseradish peroxidase and prostaglandin H synthase. (cdc.gov)
Heme10
- Cytochrome c peroxidase, or CCP, is a water-soluble heme-containing enzyme of the peroxidase family that takes reducing equivalents from cytochrome c and reduces hydrogen peroxide to water: CCP + H2O2 + 2 ferrocytochrome c + 2H+ → CCP + 2H2O + 2 ferricytochrome c CCP can be derived from aerobically grown yeast strains and can be isolated in both native and recombinant forms with high yield from Saccharomyces cerevisiae. (wikipedia.org)
- Collectively, the data show that all three members of the class I heme peroxidases can support radical formation on the distal tryptophan and that the reactivity of this radical can be controlled either by the protein structure or by the nature of the compound I intermediate. (bris.ac.uk)
- Mamoru Yamada Zymomonas mobilis ZmCytC as a peroxidase bearing three heme c -binding motifs was investigated with Δ ZmcytC constructed. (karger.com)
- M. Kathiresan and A.M. English ( 2018 ) LC-MS/MS proteoform profiling exposes cytochrome c peroxidase self-oxidation in mitochondria and functionally important hole hopping from its heme , J. Am. Chem. (concordia.ca)
- M. Kathiresan and A.M. English ( 2017 ) LC-MS/MS reveals that hole hopping in cytochrome c peroxidaseprotects its heme from oxidative modification by excess H 2 O 2 , Chem. (concordia.ca)
- M. Kathiresan and A.M. English ( 2016 ) Targeted proteomics identify metabolism-dependent interactors of yeast cytochrome c peroxidase: Implications in stress response and heme trafficking , Metallomics 8 , 434 -443. (concordia.ca)
- Peroxidase activity assay shows this protein contains heme. (nasa.gov)
- One of these genes is of particular interest, a di-heme cytochrome c peroxidase, occurs in an gene cluster with five other genes which encode for other proteins involved in electron transport. (nasa.gov)
- Iron is a part of heme, which is the active site of peroxidases that protect cells from oxidative injury by reducing peroxides to water. (medscape.com)
- Heme is also the active site of electron transport in cytochromes. (medscape.com)
Protein5
- The enzyme contains a 68-residue sequence at the N-terminus of its monomeric protein, which targets it to the inter-membrane space of the mitochondria where it can the complex with cytochrome c and where it carries out its sensor, signaling and catalytic roles. (wikipedia.org)
- The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. (nih.gov)
- In addition, the present study indicated that treatment with GP suppressed the I/R-induced increase in the pro-apoptotic protein levels of Bax and cytochrome c and the activity of caspase-3/8, as well as the I/R-induced decrease in the levels of anti-apoptotic protein Bcl‑2. (spandidos-publications.com)
- In particular, ultrafast XFEL spectroscopy is used to evaluate the methionine-S-Fe bond in cytochrome c, and its entatic control by the protein in determining function (electron transfer vs. apoptosis). (stanford.edu)
- Radiation also dramatically decreased the protein and mRNA expression of bcl-2 and increased that of bax, cytochrome c, and capase-3. (biomedcentral.com)
Enzymes3
- This phytopathogen has an abundance of secreted oxidases, peroxidases, and hydrolytic enzymes for degrading cell wall polysaccharides and lignocelluloses to penetrate into the host tissue. (biomedcentral.com)
- Peroxidase enzymes have recently been shown to catalyze the activation of a wide variety of xenobiotic compounds to reactive intermediates and these enzymes, particularly prostaglandin H synthase, have been suggested to play a role in the extrahepatic toxicity and carcinogenicity of several compounds. (cdc.gov)
- Since antioxidants are good electron donors we investigated whether BHT might be metabolically activated to BHT-quinone methide by peroxidase enzymes. (cdc.gov)
Hydrogen peroxide3
- The mechanism involves ferrous cytochrome c (Cc) providing electrons for the Cc-CcP system to reduce hydrogen peroxide to water. (wikipedia.org)
- Cytochrome c peroxidase can react with hydroperoxides other than hydrogen peroxide, but the reaction rate is much slower than with hydrogen peroxide. (wikipedia.org)
- Although the TMBZ solution is colorless, it turns bluish-green (λmax: 655 nm) in the presence of hydrogen peroxide and peroxidase. (dojindo.com)
Pseudomonas1
- In addition, MauG has sequence similarity to cytochrome c peroxidase from Pseudomonas sp. (caltech.edu)
Proteins1
- Western blotting and reverse transcriptase PCR were used to determine the expression levels of apoptosis-related proteins and genes, including bcl-2 , bax , cytochrome c , and capase-3 . (biomedcentral.com)
Ascorbate1
- CCP has high sequence identity to the closely related ascorbate peroxidase enzyme. (wikipedia.org)
Genes1
- Excessive oxidative stress alters the expression levels of apoptosis-related genes and triggers sperm apoptosis through bcl-2, bax, cytochrome c and caspase-3 signaling pathways. (biomedcentral.com)
Enzyme2
- The enzyme shows an unusual amino acid pattern compared to other peroxidase. (wikipedia.org)
- This reaction is presumably catalyzed by a cytochrome P-450 related enzyme. (cdc.gov)
Mitochondria1
- The negative charge transfer process is carried out by a complex formed between cytochrome c and cytochrome c peroxidase which occurs in the inter-membrane space of mitochondria. (wikipedia.org)
Compound1
- Functional differences between peroxidase compound I and the cytochrome P-450 reactive oxygen intermediate. (lookformedical.com)
H2O21
- therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. (nih.gov)
Activity4
- Herein, we demonstrate that cytochrome c (Cyt c ) showed peroxidase activity on IGA as a component of complexes III and IV. (biologists.com)
- The signals of IGA based on peroxidase activity were obtained using enhanced chemiluminescence solution. (biologists.com)
- An Improved Staining Procedure for the Detection of the Peroxidase Activity of Cytochrome p-450 on Sodium Dodecyl Sulfate Polyacrylamide Gels. (dojindo.com)
- 2,3,7,8-Tetrachlorodibenzo- p -dioxin as an antiestrogen: Effect on rat uterine peroxidase activity. (cdc.gov)
Porphyrin1
- This is different from most peroxidases, which have the second oxidising equivalent on the porphyrin instead. (wikipedia.org)
Peroxides1
- Since the decarboxylation of peroxyacids is diagnostic of peroxide homolysis, these results indicate a fundamentally different manner of processing of peroxides by cytochrome P-450 than by the peroxidases. (lookformedical.com)
Liver2
- Preparation of Partially Purified, Lipid-depleted Cytochrome P-450 and Reduced Nicotinamide Adenine Dinucleotide Phosphatecytochrome c Reductase from Rat Liver Microsomes. (dojindo.com)
- A multicompartment geometric model of the liver in relation to regional induction of cytochrome P450s. (cdc.gov)
Properties1
- How Does Replacement of the Axial Histidine Ligand in Cytochrome c Peroxidase by N δ -Methyl Histidine Affect Its Properties and Functions? (bvsalud.org)
Activation1
- Structural characterization of cardiolipin-driven activation of cytochrome C into a peroxidase and membrane perturbation. (cdc.gov)
Enzymes6
- In this work, we present high resolution crystal structures of both Compound I and Compound II intermediates in two different heme peroxidase enzymes, cytochrome c peroxidase and ascorbate peroxidase, allowing direct and accurate comparison of the bonding interactions in the different intermediates. (rcsb.org)
- The target of this study was to investigate a role of CYP and peroxidase enzymes in ellipticine oxidative activation in rats, a suitable model mimicking the fate of ellipticine in humans, in details. (nel.edu)
- The contribution of pulmonary and renal CYP- and peroxidase enzymes to ellipticine metabolic activation is investigated and compared with that found in the liver. (nel.edu)
- The results demonstrate that whereas CYP enzymes of 1A and 3A subfamilies are the major enzymes activating ellipticine in rat livers, peroxidase COX plays a significant role in this process in lungs and kidneys. (nel.edu)
- In terms of enzymes, measurements, super-oxide dismutase activity (SOD), peroxidase activity, Glucoamylase/Beta-glucanasase activity, Glucose 2-oxdase activity, Cytochrome "P-450", Cytochrome P 450 reductase were taken in the absence of proteolytic enzymes and in the presence of proteolytic enzymes. (mycologyresearch.com)
- Along with animal peroxidases, these enzymes belong to a group of peroxidases containing a heme prosthetic group (ferriprotoporphyrin IX), which catalyzes a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. (unl.edu)
Yeast cytochrome c peroxida2
- The inability of imidazole to complement function in the axial histidine deletion mutant, H175G, of yeast cytochrome c peroxidase has been an intriguing but unresolved issue that impacts our understanding of the role of axial ligands in heme catalysis. (ox.ac.uk)
- 17. Influence of protein environment on magnetic circular dichroism spectral properties of ferric and ferrous ligand complexes of yeast cytochrome c peroxidase. (nih.gov)
Catalases1
- Comparative studies then provided insights into their physiology, especially those with ions as coenzymes - haemoglobins, cytochromes, peroxidases, catalases. (animalresearch.info)
Horseradish4
- Plant peroxidase such as horseradish peroxidase and pineapple peroxidase B have low lysine, tryptophan, and tyrosine contents and high cysteine content. (wikipedia.org)
- Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. (ibiomagazine.org)
- Class II includes ligninase and other extracellular fungal peroxidases, while class III is comprised of classic extracellular plant peroxidases, like horseradish peroxidase. (unl.edu)
- Horseradish peroxidase and related secretory plant peroxidases. (unl.edu)
Lignin1
- Class III peroxidases are found in the extracellular space or in the vacuole in plants where they have been implicated in hydrogen peroxide detoxification, auxin catabolism and lignin biosynthesis, and stress response. (unl.edu)
Hydrogen peroxide to water3
- Cytochrome c peroxidase, or CCP, is a water-soluble heme-containing enzyme of the peroxidase family that takes reducing equivalents from cytochrome c and reduces hydrogen peroxide to water: CCP + H2O2 + 2 ferrocytochrome c + 2H+ → CCP + 2H2O + 2 ferricytochrome c CCP can be derived from aerobically grown yeast strains and can be isolated in both native and recombinant forms with high yield from Saccharomyces cerevisiae. (wikipedia.org)
- The mechanism involves ferrous cytochrome c (Cc) providing electrons for the Cc-CcP system to reduce hydrogen peroxide to water. (wikipedia.org)
- it forms a complex with cytochrome c to catalyze the reduction of hydrogen peroxide to water. (unl.edu)
Oxidative2
- The results suggest the involvement of ZmCytC in the aerobic respiratory chain via the cytochrome bc 1 complex in addition to the previously proposed direct interaction with ubiquinol and its contribution to protection against oxidative stress. (karger.com)
- Iron is a part of heme, which is the active site of peroxidases that protect cells from oxidative injury by reducing peroxides to water. (medscape.com)
Intracellular1
- Ouabain triggered transient neuronal cell swelling followed by cell shrinkage, accompanied by intracellular Ca 2+ and Na + increase, K + decrease, cytochrome c release, caspase-3 activation, and DNA laddering. (jneurosci.org)
Electron2
- Thus, the highly conserved Tyr67 is a likely electron-donor (radical acceptor) in the oxygenase half-reaction of the cyt c/CL peroxidase complex. (cdc.gov)
- Heme is also the active site of electron transport in cytochromes. (medscape.com)
Mitochondrial1
- Formation of cytochrome c (cyt c)/cardiolipin (CL) peroxidase complex selective toward peroxidation of polyunsaturated CLs is a pre-requisite for mitochondrial membrane permeabilization. (cdc.gov)
Protein2
- The enzyme contains a 68-residue sequence at the N-terminus of its monomeric protein, which targets it to the inter-membrane space of the mitochondria where it can the complex with cytochrome c and where it carries out its sensor, signaling and catalytic roles. (wikipedia.org)
- Subsequently, results from an in vitro epilepsy model demonstrated that overexpression of miR‑128 promoted nerve cell apoptosis, increased the protein expression of tumor protein p53, BCL2 associated X (Bax) and Cytochrome c, and enhanced caspase‑3/9 activity, whereas it suppressed the protein expression of sirtuin 1 (SIRT1). (spandidos-publications.com)
Proximal3
- 2. Elucidating the role of the proximal cysteine hydrogen-bonding network in ferric cytochrome P450cam and corresponding mutants using magnetic circular dichroism spectroscopy. (nih.gov)
- 9. Model Complexes Elucidate the Role of the Proximal Hydrogen-Bonding Network in Cytochrome P450s. (nih.gov)
- 11. Roles of the proximal hydrogen bonding network in cytochrome P450cam-catalyzed oxygenation. (nih.gov)
Tyrosine2
- Topography of tyrosine residues and their involvement in peroxidation of polyunsaturated cardiolipin in cytochrome c/cardiolipin peroxidase complexes. (cdc.gov)
- Tyrosine residues - via the generation of tyrosyl radicals (Tyr) - are likely reactive intermediates of the peroxidase cycle leading to CL peroxidation. (cdc.gov)
Caspase2
- In conclusion, the present findings indicated that anti‑miR‑128 may exert neuroprotective effects in epilepsy, through the SIRT1/p53/Bax/Cytochrome c/caspase signaling pathway. (spandidos-publications.com)
- Potassium channel blockers or high K + medium, but not Ca 2+ channel blockade, prevented cytochrome c release, caspase activation, and DNA damage. (jneurosci.org)
Ligand1
- Replacement of the axial histidine ligand with imidazole in cytochrome c peroxidase. (ox.ac.uk)
Species2
- However, while this form is fully reactive with peroxide, the reaction with cytochrome c remains inefficient, perhaps implicating the altered Trp-191 radical species. (ox.ac.uk)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Cytochrome C, Somatic (CYCS) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (ibiomagazine.org)
Structural1
- Peroxidase activity and structural transitions of cytochrome c bound to cardiolipin-containing membranes. (cdc.gov)
Membrane1
- The negative charge transfer process is carried out by a complex formed between cytochrome c and cytochrome c peroxidase which occurs in the inter-membrane space of mitochondria. (wikipedia.org)
Mutants1
- We used mutants of horse heart cyt c in which each of the four Tyr residues was substituted for Phe and assessed their contribution to the peroxidase catalysis. (cdc.gov)
Reaction1
- Cytochrome c peroxidase can react with hydroperoxides other than hydrogen peroxide, but the reaction rate is much slower than with hydrogen peroxide. (wikipedia.org)
Plant3
- Heme-dependent peroxidases similar to plant peroxidases. (unl.edu)
- The plant peroxidase-like superfamily is found in all three kingdoms of life and carries out a variety of biosynthetic and degradative functions. (unl.edu)
- Secretory peroxidases belong to class III of the plant heme-dependent peroxidase superfamily. (unl.edu)
Cells1
- Freshly isolated hPT cells had relatively high activities of γ-glutamyltransferase, γ-glutamylcysteine synthetase, glutathione S -transferase (GST), glutathione disulfide reductase, and GSH peroxidase. (aspetjournals.org)
Class1
- Class III peroxidases contain four conserved disulphide bridges and two conserved calcium binding sites. (unl.edu)
Description2
- Description: A sandwich quantitative ELISA assay kit for detection of Rat Cytochrome C, Somatic (CYCS) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (ibiomagazine.org)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Cytochrome C, Somatic (CYCS) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (ibiomagazine.org)