Wendy Macklin's laboratory has recently developed a technique to better separate the demyelination and remyelination phases of the cuprizone model. (msdiscovery.org)
MPF was measured in vivo using the fast single-point method in three animal groups (control, cuprizone-induced demyelination, and remyelination after cuprizone withdrawal) and compared to quantitative immunohistochemistry for myelin basic protein (MBP), myelinating oligodendrocytes (CNP-positive cells), and oligodendrocyte precursor cells (OPC, NG2-positive cells) in the corpus callosum, caudate putamen, hippocampus, and cortex. (tsu.ru)
Changes in neurosteroidogenesis during demyelination and remyelination in cuprizone-treated mice. (bvsalud.org)
Myelin5
RESULTS: Cuprizone caused loss of myelin basic protein (MBP) immunofluorescence in the CC suggesting demyelination. (ox.ac.uk)
Treatment with a new TREM2 agonistic antibody promoted the clearance of myelin debris in the cuprizone model of CNS demyelination. (quanterix.com)
These studies revealed that Gpr37−/− mice exhibit dramatically increased loss of myelin in response to cuprizone, yet do not show any increased loss of oligodendrocyte precursor cells or mature oligodendrocytes. (smith.edu)
To further investigate whether myelin defects can cause plaque deposition, they induced acute demyelination in 5xFAD mice by feeding them Cuprizone. (biologists.com)
Quantitative analysis of lipid debris accumulation caused by cuprizone induced myelin degradation in different CNS areas. (kodalyvilaga.hu)
Remyelination in the cuprizone1
CONCLUSIONS: This study reveals a uniquely regulated inflammatory response in the SVZ and shows that Gal-3 participates in remyelination in the cuprizone model. (ox.ac.uk)
Mice10
METHODS: We studied the inflammatory response to cuprizone in the SVZ and CC in Gal-3 knockout mice using immunohistochemistry and with the in vitro neurosphere assay. (ox.ac.uk)
BrdU+ SVZ cell numbers also decreased in the SVZ after cuprizone, and this effect was significantly greater at 3 weeks in Gal-3 (-/-) mice compared to WT, suggesting Gal-3 normally limits SVZ cell emigration following cuprizone treatment. (ox.ac.uk)
When administered orally in mice, cuprizone, a copper chelator, causes rapid demyelination and gliosis, or rapid proliferation of glia subtypes. (msdiscovery.org)
In the acute cuprizone paradigm, male C57BL/6 mice at 6 to 9 weeks of age are fed a diet of chow mixed with 0.2% cuprizone over the course of 6 weeks. (msdiscovery.org)
Investigators inject cuprizone-fed mice with rapamycin, a drug that blocks mTOR and spontaneous remyelination, allowing better quantification of oligodendrocyte turnover. (msdiscovery.org)
Ten-week-old C57BL/6J, TLR4 − / − , and MyD88 − / − mice received 0.25% cuprizone for 6 weeks and were treated daily with 25 mg/kg laquinimod or vehicle. (neurology.org)
Defective remyelination can be recapitulated in vivo in mice receiving systemic administration of pharmacological HDAC inhibitors during cuprizone treatment and is consistent with in vitro results showing defective differentiation of oligodendrocyte progenitors after silencing specific HDAC isoforms. (nih.gov)
Male C57BL/6 mice fed 0.3% cuprizone for 12 weeks exhibited CC atrophy with chronic demyelination, astrogliosis, and microglial activation. (cam.ac.uk)
As loss of MAG has previously been shown to result in increased susceptibility to brain insults, we additionally assessed Gpr37-knockout (Gpr37−/−) vs. wild-type mice in the cuprizone model of demyelination. (smith.edu)
Although this copper chelator could possibly interfere with plaque formation, Cuprizone-treated mice showed a substantial increase of amyloid aggregates in regions of strong demyelination. (biologists.com)
Oligodendrocyte3
Cuprizone treatment is the most frequently used among the toxicant-induced MS models, which include lysolecithin and ethidium bromide, and is used to study mechanisms of oligodendrocyte turnover, astrogliosis, and microgliosis ( Blakemore and Franklin, 2008 ). (msdiscovery.org)
To test whether Toll-like receptor (TLR) signaling plays a key role for reduced nuclear factor B (NF-κB) activation after laquinimod treatment in the model of cuprizone-induced demyelination, oligodendrocyte apoptosis, inflammation, and axonal damage. (neurology.org)
Cuprizone-induced oligodendrocyte loss and demyelination impairs recording performance of chronically implanted neural interfaces. (kozailab.com)
Corpus Callosum2
BACKGROUND: Cuprizone leads to demyelination of the corpus callosum (CC) and activates progenitor cells in the adjacent subventricular zone (SVZ), a stem cell niche which contributes to remyelination. (ox.ac.uk)
By the third week of cuprizone feeding, consistent demyelination can be observed in the corpus callosum, the largest white matter tract in the mouse brain. (msdiscovery.org)
C57BL1
Different rodent strains react in idiosyncratic ways to cuprizone, which is best characterized in the C57BL/6 strain. (msdiscovery.org)
Murine2
This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. (tsu.ru)
This study aimed to identify associations between neurogenesis in the neurogenic niches, oligodendrogenesis, and myelination in the murine cuprizone demyelination/remyelination model. (ismrm.org)
Chronic demyelination1
Prolonged cuprizone exposure induces chronic demyelination and impairs the capacity of the brain to repair. (msdiscovery.org)
Model6
The cuprizone mouse model captures several aspects of MS pathology, bypassing the autoimmune component in other preclinical models. (msdiscovery.org)
Gender is another rate-limiting factor to consider when using the cuprizone model. (msdiscovery.org)
Our results confirm that laquinimod prevents demyelination in the cuprizone mouse model for multiple sclerosis via downregulation of NF-κB activation. (neurology.org)
Effects of recombinant human erythropoietin in the cuprizone mouse model of de- and remyelination. (mpg.de)
The neurotoxicant-induced, cuprizone model of demyelination is ideally suited for the analysis of inflammatory events. (elsevierpure.com)
To investigate the therapeutic efficacy, pharmacokinetics, and toxicity profile of novel drug treatments using the Experimental Autoimmune Encephalomyelitis (EAE) mouse model, Cuprizone mouse model, and in-vitro models. (edu.au)
Induces1
Cuprizone demyelination induces a unique inflammatory response in the subventricular zone. (ox.ac.uk)
Oligodendrogenesis1
This system reliably replicated human analysis, outperformed traditional analytic approaches, and extracted injury and repair dynamics at multiple cortical depths, establishing that oligodendrogenesis after cuprizone-mediated demyelination is suppressed in deeper cortical layers. (johnshopkins.edu)
Proliferation2
Inflammation can regulate SVZ proliferation and indeed the number of phosphohistone H3+ (PHi3+) cells decreased in the SVZ but increased in the CC in both genotypes after cuprizone treatment. (ox.ac.uk)
2020 ) Role of Macrophage Colony-Stimulating Factor Receptor on the Proliferation and Survival of Microglia Following Systemic Nerve and Cuprizone-Induced Injuries. (neurotree.org)
Focal1
Unlike other toxicant-induced models, which are introduced in the brain by stereotaxic microinjections and result in focal demyelination, oral administration of cuprizone produces a global insult. (msdiscovery.org)
Withdrawal1
Demyelination on exposure to cuprizone is accompanied by predictable microglial activation and astrogliosis, and, after cuprizone withdrawal, this activation reproducibly diminishes during remyelination. (elsevierpure.com)
Exposure1
The animal's gender, age, and exposure time to cuprizone are important determinants of the reproducibility and sequence of de- and remyelination events. (msdiscovery.org)
Inflammation1
Cuprizone increased the density of CD45+/Iba1+ microglial cells and also increased Gal-3 expression in the CC. Surprisingly, the number of Gal-3+ and CD45+ cells decreased in the SVZ after cuprizone, suggesting inflammation was selectively reduced therein. (ox.ac.uk)
Mouse1
White matter abnormality is one of the early neuropathological events in AD, thus we will use cuprizone-induced demyelination to assess the protective effect of SPI1 in mouse brain. (brightfocus.org)
Cells1
Outcome of cuprizone on thymic epithelial cells. (itkinhibitor.com)
Sequence1
To investigate the impact of episodes of reversible demyelination on long-term locomotor performance and neuro-axonal integrity, cuprizone-treated animals were monitored using motor skill sequence (MOSS), to observe whether a functional recovery was attained and maintained in the long-term. (uni-goettingen.de)
Weeks3
With cuprizone alone, her laboratory documents 30% to 40% myelination at 6 weeks, with remyelination complete by 3 to 5 weeks off the toxicant. (msdiscovery.org)
With cuprizone and rapamycin together, the group detects 0% myelination at 6 weeks and complete remyelination 7 weeks later. (msdiscovery.org)
Syngeneic iNSCs were transplanted into the CC after ending cuprizone and perfused for neuropathology 2 weeks later. (cam.ac.uk)
In the present study, we sought to determine the role of microglial Hv1 proton channels in a mouse model of cuprizone-induced demyelination, a model for MS. Following cuprizone exposure, wild-type mice presented obvious demyelination, decreased myelin basic protein expression, loss of mature oligodendrocytes, and impaired motor coordination in comparison to mice on a normal chow diet. (nih.gov)
These rescued phenotypes in Hv1(-/-) mice in cuprizone-induced demyelination is accompanied by reduced ROS production, ameliorated microglial activation, increased oligodendrocyte progenitor cell (NG2) proliferation, and increased number of mature oligodendrocytes. (nih.gov)
These results demonstrate that the Hv1 proton channel is required for cuprizone-induced microglial oxidative damage and subsequent demyelination. (nih.gov)
In this study, the contribution of myelin to both T(2)* and frequency contrasts is investigated using a mouse model of demyelination based on a cuprizone diet. (nih.gov)
Evidence from a cuprizone-induced model of demyelination, in vitro and in vivo T-cell assays and EAE adoptive transfer experiments indicated that the observed efficacy of this drug results directly from an enhancement of remyelination rather than immune suppression. (ca.gov)
The effect of ACT-1004-1239 was evaluated in the myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) and the cuprizone-induced demyelination mouse models. (listlabs.com)
Conversely, no GPR17 upregulation was found in a model characterized by a much lower degree of inflammation, i.e. cuprizone-induced demyelination. (unimi.it)
Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination. (msdiscovery.org)
Our findings revealed that galectin-4 is transiently localized to axons in demyelinated areas upon cuprizone-induced demyelination. (amsterdamumc.org)
To identify methods for a differentiation of demyelination, inflammation, and axonal damage we developed a novel mouse model combining cuprizone-induced demyelination and experimental autoimmune encephalomyelitis. (goettingen-research-online.de)
In DTI, axonal damage and cellular infiltration led to a reduction of the axial diffusivity, whereas primary demyelination after cuprizone treatment was reflected by changes in radial but not axial diffusivity. (goettingen-research-online.de)
Remyelination3
1. We use the cuprizone (CPZ) model of experimental de- and remyelination to examine (i) microRNA, (ii) transcriptome, (iii) and proteome/post-translational proteome of de- and remyelination in the brain. (sdu.dk)
9. CZ-7, a new derivative of Claulansine F, promotes remyelination induced by cuprizone by enhancing myelin debris clearance. (nih.gov)
15. Electroacupuncture Promotes Remyelination after Cuprizone Treatment by Enhancing Myelin Debris Clearance. (nih.gov)
Microglia2
We are currently investigating the mechanisms by which microglia contribute to the induction and resolution of brain damage using mouse models of multiple sclerosis (EAE, cuprizone model) and neurodegeneration , e.g. (uniklinik-freiburg.de)
Remarkably, microglia/macrophages in cuprizone-demyelinated areas also harbor galectin-4, as also observed in activated microglia/macrophages that are present in active MS lesions and in inflammatory infiltrates in chronic-relapsing experimental autoimmune encephalomyelitis. (amsterdamumc.org)
Brain1
1991. A comparison of spongiosis induced in the brain by hexachlorophene, cuprizone and triethyl tin in the Sprague-Dawley rat. (nih.gov)
Vivo1
Comparison of in-vivo and in-vitro data showed that, although tissue T(2)* values were reduced by formalin fixation, gray-white matter frequency contrast was relatively unaffected and fixation had a negligible effect on cuprizone-induced changes in T(2)* and frequency contrasts. (nih.gov)