• Long-term imprecision was less than 5% for neutrophils, eosinophils, basophils, lymphocytes and monocytes. (bmj.com)
  • Eosinophil levels decreased by 54.1% ( p = 0.006), whereas levels of neutrophils, monocytes, basophils, and NK cells were not significantly altered. (neurology.org)
  • Conclusion: Vitamin E-bonded dialyzers may ameliorate eosinophilia through a mechanism mediated by a decrease in IL-5 secretion by CD4-positive lymphocytes. (dadamo.com)
  • IgE reactivity and basophil activation studies were performed in clinically defined mugwort-allergic patients. (nih.gov)
  • Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. (nih.gov)
  • Diagnostic testing included serum levels of Ses i 1-specific IgE, skin prick test with high-protein extract, and basophil reactivity (% induced CD63 expression) for each patient. (tau.ac.il)
  • Western blotting, inhibition ELISA and basophil activation tests were used to examine IgE reactivity. (oit101.org)
  • PF670462 or dexamethasone also ameliorated allergic symptoms in a mouse model of allergic rhinitis and downregulated allergen-specific basophil reactivity in patients with allergic rhinitis. (elsevierpure.com)
  • Conclusion The study confirms the increasingly interesting role emerging for blood eosinophils and basophils in different CRSwNP endotypes. (unipd.it)
  • Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
  • BACKGROUND: IL-5 plays a central role in eosinophil and basophil differentiation, exerting its effects through the IL-5 receptor (IL-5Ralpha). (dadamo.com)
  • Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)
  • OBJECTIVE: Because all-trans retinoic acid (ATRA) is known to modulate some aspects of hemopoietic differentiation, we examined the effects of ATRA on eosinophil-basophil differentiation and IL-5Ralpha expression. (dadamo.com)
  • RESULTS: In semisolid methylcellulose cultures of normal human bone marrow, ATRA (10(-6) mol/L) selectively suppressed eosinophil-basophil colony-forming units but had no effect on granulocyte-macrophage colony-forming units. (dadamo.com)
  • Similarly, ATRA (10(-6) mol/L) inhibited eosinophil-basophil differentiation of cord blood CD34(+) cells in liquid culture, whereas neutrophil differentiation proceeded without impediment. (dadamo.com)
  • CONCLUSIONS: These findings indicate that retinoids can differentially regulate expression of IL-5Ralpha, but not GM-CSF receptor, and that these effects have functional consequences in vitro on eosinophil and basophil differentiation. (dadamo.com)
  • Blood eosinophil-to-basophil ratio in patients with sinonasal polyps: does it have a clinical role? (unipd.it)
  • Background In a preliminary study, eosinophil and basophil counts were recently calculated in chronic rhinosinusitis with nasal polyps (CRSwNP) using conventional histological and immunohistochemical methods: the tissue eosinophil-to-basophil ratio differed in the CRSwNP endotypes considered. (unipd.it)
  • Objective The aim of the present study was to compare the blood eosinophil-to-basophil ratio (bEBR) in a large series of CRSwNP with those in a control group of consecutive rhinological patients with no evidence of nasal/paranasal or systemic inflammatory disorders. (unipd.it)
  • The eosinophil and basophil counts were significantly and directly correlated in the CRSwNP cases (p=0.0000). (unipd.it)
  • Eosinophil primarily combat parasitic infections and play a role in allergic reactions, while basophils release chemicals, such as histamine, which are involved in inflammation and allergic responses. (alliedacademies.org)
  • At a bee venom concentration of 100 ng/mL, the mean proportion of basophils expressing CD63 was 56% in reactors and 13.2% in nonreactors (P=.0321). (jiaci.org)
  • The captured basophils were activated using an anti-FceRI antibody and the basophil identification marker CD203c and the activation marker CD63 were detected using fluorescence microscopy. (kth.se)
  • We detected a significant difference in CD63-expression between the negative control and allergen-activated basophils from allergic patients but no difference between the negative control and the non-relevant allergen (an allergen to which the patient had no IgE antibodies). (kth.se)
  • Basophils did not upregulate CD63 after passage through a microfluidic chip, but there was a slight but significant activation after crosslinking of CD203c, which is the surface marker used for basophil-specific capture in miBAT, giving one potential factor for the background activation. (kth.se)
  • IgE-dependent (anti-IgE) basophil degranulation after crosslinking of adhesion molecules, to mimic adhesion before transmigration into tissue, showed a significant decrease in CD63-expression compared to anti-IgE activation, which indicate a regulatory function. (kth.se)
  • Cytokine stimulation followed by IgE-independent (fMLP) basophil degranulation on the other hand showed a significantly increased CD63-expression compared to non-primed fMLP activation. (kth.se)
  • In conclusion, we have developed a novel microfluidic-based technique (miBAT) able to detect basophil activation (CD63-expression) using allergens in allergic patients. (kth.se)
  • Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). (kth.se)
  • Results PF670462 or corticosterone (or dexamethasone) suppressed IgE-mediated allergic reactions in mouse bone marrow-derived mast cells or basophils and passive cutaneous anaphylactic reactions in mice in association with increased PER2 levels in mast cells or basophils. (elsevierpure.com)
  • The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics. (lu.se)
  • In paper I, we developed a novel microfluidic immunoaffinity-based basophil activation test (miBAT) assay to investigate whether it was possible to capture and activate basophils from whole blood in a microfluidic chip. (kth.se)
  • In the discovery dataset, multivariable MR analysis revealed that sum basophil neutrophil counts had an independent causal effect on the occurrence of HCC, with the sum basophil neutrophil counts as follows: (OR = 0.437, P = 0.003, CI 0.252-0.757). (biomedcentral.com)
  • Similarly, in the validation dataset, total basophil neutrophil counts were also been identified as an independent risk factor for HCC. (biomedcentral.com)
  • The sum basophil neutrophil counts were as follows: (OR = 0.574, P = 0.021, CI 0.358-0.920). (biomedcentral.com)
  • In the European population, genetically predicted lower total basophil neutrophil counts might be an independent risk factor for HCC. (biomedcentral.com)
  • In conclusion, AAV patients exhibit a skewing of different neutrophil and monocyte subpopulations that are associated with disease subtypes, disease activity, rituximab treatment, and propensity to relapse. (lu.se)
  • Blood sample analyses included IgE against dog dander, dog allergen molecules, other airborne and food allergens, basophil activation and white blood cell counts. (ersjournals.com)
  • In the absolute counts of differentials, the AUC was the highest in basophils (0.982), followed by immature granulocytes (IGs) (0.975), which had cut-off values of 0.43 × 10 9 /L and 0.46 × 10 9 /L, respectively. (elsevierpure.com)
  • In the validation, the absolute basophil counts were elevated in 280 samples from 96 cases, including 22 CML cases. (elsevierpure.com)
  • However, at a concentration of 1000 ng/mL, no significant differences in basophil activation were observed between reactors and nonreactors. (jiaci.org)
  • The main difference between mast cell and basophil is that a typical mast cell contains around 1000 small granules whereas a basophil contains around 80 large granules . (pediaa.com)
  • Furthermore, the number of granules in a mast cell cytoplasm is around 1000 while the number of granules in a basophil is around 80. (pediaa.com)
  • The other statistically significant differences between control and treated animals (mean corpuscular haemoglobin concentration in males dosed with 100 mg/kg bw/day, erythrocytes in females of the same group and basophils in females dosed with 1000 mg/kg bw/day) were of minimal severity and/or not dose-related, therefore considered of no toxicological significance. (europa.eu)
  • Methods and Results: The role of tyrosine kinases in human FceRI signaling was explored by using human basophils isolated by Percoll gradient centrifugation followed by negative and/or positive selection with antibody-coated magnetic beads. (uncg.edu)
  • The presence of Lyn, Syk, and Zap-70 in basophils was confirmed by Western blotting in lysates of highly purified basophils and independently by confocal fluorescence microscopy in cells labeled simultaneously with kinase-specific antibodies and with the basophil-specific antibody 2D7. (uncg.edu)
  • Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. (kth.se)
  • When re-exposure occurs, inflammatory mediators like histamine are released from basophils and mast cells. (fsahq.org)
  • Many of the cells that are activated by HRF/TCTP participate in the allergic response, leading to the conclusion that the extracellular functions of HRF/TCTP could exacerbate the allergic, inflammatory response. (benthamopen.com)
  • Conclusions: IL-17A promotes the release of IL-1β and IL-18 by activating the NLRP3 inflammasome and aggravates the severity of the disease in CRSwNP. (bvsalud.org)
  • Mast cell and basophil are two types of white blood cells which contain granulocytes . (pediaa.com)
  • We tested sera for their ability to release histamine from human basophils and to activate rat basophil leukemia cells that were transfected with the alpha subunit of the IgE receptor. (qxmd.com)
  • HRF/TCTP has been shown to activate multiple human cells including basophils, eosinophils, T cells and B cells. (benthamopen.com)
  • The role of basophil activation test and component-resolved diagnostics in the workup of egg allergy in children at low risk for severe allergic reactions: A real-life study. (bvsalud.org)
  • Our study aimed to evaluate the utility of the basophil activation test (BAT) and component-resolved diagnostic in the diagnostic workup of children with egg allergy . (bvsalud.org)
  • A cluster of these children with enhanced CST1 expression showed lower forced expiratory volume in 1 s, increased bronchial hyperreactivity, pronounced eosinophilia and higher basophil allergen threshold sensitivity compared with other children sensitised to dog dander. (ersjournals.com)
  • Univariate analyses (UVA) and multivariable analyses (MVA) identified worse clinical outcomes (CO) associated with elevated basophils and basophil-to-lymphocyte ratio (BLR). (emory.edu)
  • Based on MVA, elevated basophils (defined as ≥0.1, optimal cut) were associated with a hazard ratio (HR) of 3.51 (95% CI 1.65-7.43, P 0.001) for OS and HR of 1.88 (95% CI 1.05-3.38, P 0.034) for PFS. (emory.edu)
  • In contrast, basophil refers to a type of immune cell that has granules (small particles) with enzymes that are released during allergic reactions and asthma. (pediaa.com)
  • Objective We determined whether pharmacologically resetting the molecular clock in mast cells or basophils to times when FcϵRI signaling was reduced (ie, when core circadian protein period 2 [PER2] is upregulated) resulted in suppression of IgE-mediated allergic reactions. (elsevierpure.com)
  • Conclusion Pharmacologically resetting the molecular clock in mast cells or basophils to times when FcϵRI signaling is reduced can inhibit IgE-mediated allergic reactions. (elsevierpure.com)
  • Similarly, 1-acyl-2-acetyl-GPC (0.1 to 10 μmol/L) caused a concentration-dependent inhibition of PAF-activated histamine secretion (inhibitory concentration of 50% ≅ 0.2 μmol/L). Conclusions: Our data suggest that 1-acyl-2-acetyl-GPC may represent, under certain circumstances, a modulator of human basophil mediator release via mechanisms shared with PAF. (arizona.edu)
  • Opinion, findings and conclusions expressed by the original authors of items included in the update, or persons quoted therein, are strictly their own and are in no way meant to represent the opinion or views of CDC or DHHS. (cdc.gov)
  • In paper II, we further investigated whether it was possible to detect a dose-dependent allergen activation for basophils captured in a microfluidic chip. (kth.se)
  • Purified parvalbumin reacted with IgE of more than 95% of individuals allergic to fish, induced dose-dependent basophil histamine release and contained, on average, 83% of the IgE epitopes present in other fish species. (unipv.it)
  • These new data are in agreement with our original conclusions, but we believe fully address the reviewer concerns and significantly strengthen the manuscript. (authorea.com)
  • Immature mast cells leave the bone marrow into the circulation and then matures when entering a tissue while mature basophils leave the bone marrow. (pediaa.com)
  • Egg white-specific IgE, skin testing and basophil activation decreased similarly after BE and egg OIT. (egginfo.co.uk)
  • Food additives can exacerbate ADHD symptoms and cause non-immunoglobulin E-dependent histamine release from circulating basophils. (nih.gov)
  • Basophils release histamine, which causes blood vessels to dilate and increases blood flow to the site of infection or injury, facilitating the arrival of other immune cells [ 4 ]. (alliedacademies.org)
  • Parvalbumin was purified to homogeneity, analysed by mass spectrometry and circular dichroism (CD) spectroscopy, and its allergenic activity was analysed by IgE binding and basophil histamine release tests. (unipv.it)
  • Methods: To study the biologic activity of 1-acyl-2-acetyl-GPC, we compared its effects and those of PAF on histamine and leukotriene C 4 (LTC 4 ) release from human mixed leukocytes that contained basophils. (arizona.edu)
  • We investigated usefulness of the basophil activation test (BAT) in predicting the outcome of sting challenge in bee venom allergic patients after VIT. (jiaci.org)
  • To demonstrate a specific IgE mechanism of basophil activation, BAT with wortmannin pre-treatment was performed in four LAR patients. (biomedcentral.com)
  • Conclusion: Ses i 1 IgE and BAT used together can decrease the need for OFC in most SFA patients. (tau.ac.il)
  • Although splenectomy has led to brief responses in patients with mast cell leukemia, no firm conclusions as to the efficacy of this treatment are possible. (wikipedia.org)
  • Conclusions: Our immunological approach enabled us to fully characterize the sensitization pattern of 6 sesame seed-allergic patients. (wur.nl)
  • Conclusions: Although we found a trend favoring full dose cabozantinib, this is likely due to worse baseline disease characteristics among patients starting on a reduced dose. (emory.edu)
  • Conclusion : l'utilisation du coefficient 6000 dans l'estimation de la parasitémie, a entraîné une sous-estimation significative de la charge parasitaire et n'était pas adaptée à la réalité des patients consultés au CHU-MEL. (bvsalud.org)
  • Conclusions The CICS has proven to be a reliable and accurate tool in the differential WBC count on peripheral blood smears and provides small laboratories with a 24 h available real-time digital differential WBC count on peripheral blood smears and consultation for patients in remote locations. (bmj.com)
  • Methods: We analysed the incidence and severity of SAEs during VIT in patients undergoing VIT at the University Hospital Golnik in 2005-2016 and analysed clinical (age, sex, severity of the initial reaction) and immunological (specific IgE for wasp and bee venom, recombinant IgE, basal tryptase, basophil activation test) risk factors for SAEs. (5dok.info)
  • White blood cell (WBC) count and differential count showed similar results (r range = 0.8605-0.9821) for all, with exception of basophils which showed modest correlation (r = 0.4827 for patients and 0.5758 for normal donors). (elsevierpure.com)
  • The aims of this thesis were to improve the existing BAT using microfluidic techniques to enable fast and cheap point-ofcare (POC) diagnostics as well as to further study basophil adhesion and activation for a better understanding of basophil behavior and regulation both in vivo and in vitro in a microfluidic chip. (kth.se)
  • The immunological background of the sesame seed and tree nut IgE sensitization was characterized with Western blotting and a basophil activation test (BAT). (wur.nl)
  • In paper III, we studied basophil adhesion and activation to better understand both basophil function as well as the effect that basophil capture and stimulation in a microfluidic chip has on the cell. (kth.se)
  • Background: In human blood basophils, cross-linking the high-affinity IgEreceptor FceRI with multivalent antigen activates a signaling pathway leading to Ca2+ mobilization, actin polymerization, shape changes, secretion, and cytokine production. (uncg.edu)
  • Conclusion: We showed that peripheral immune signatures reflected dynamics of clinical outcome during OFC with peanut. (sdu.dk)
  • Conclusion: We conclude that elevated baseline basophils and BLR are associated with worse clinical outcomes in mHSPC. (emory.edu)
  • En conclusion, l'utilisation d'huile de margousier, substance plutôt inoffensive pour l'environnement, est une alternative au larvicide d'anophèle. (who.int)
  • Prick tests and basophil activation tests were performed to several pharmacological and cosmetic products, but only those containing PEGs and their derivatives were positive. (biomedcentral.com)
  • Conclusions: Our data demonstrate the association of anti-IgE-stimulated, protein-tyrosine phosphorylation by a cascade of tyrosine kinases, including Zap-70 as well as Lyn and Syk, with the initiation of FceRI-mediated signaling in human basophils. (uncg.edu)
  • CONCLUSION: Ingested peanut protein is absorbed systemically and retains its immunoreactive capacity in human serum. (lu.se)
  • CONCLUSIONS: Purified carp parvalbumin represents an important cross reactive food allergen. (unipv.it)
  • The aim of the study was to evaluate the presence of specific D. pteronyssinus (DP) activation of basophils by basophil activation test (BAT) in subjects with confirmed LAR. (biomedcentral.com)
  • NMFI and PXF-based quantification showed significant correlations with basophils in the aorta, with the most prominent association for the abdominal segment (r=0.85, P=0.03). (acrabstracts.org)
  • Basophil is a type of white blood cell found in the circulation. (pediaa.com)
  • Conclusions Overexpression of CST1 is associated with more severe allergic airway disease in children sensitised to dog dander. (ersjournals.com)
  • Also, the location where they occur is a major difference between mast cell and basophil. (pediaa.com)
  • Furthermore, the nucleus of the mast cell is round while the nucleus of the basophil is lobed. (pediaa.com)
  • Therefore, the main difference between mast cell and basophil is the type of granules in the cytoplasm and their location. (pediaa.com)
  • FceRI cross-linking of more than 95% pure basophil preparations activates the protein-tyrosine kinases Lyn and Syk, previously linked to FceRI-coupled rodent mast cell activation, as well as Zap-70, previously implicated in T-cell receptor signaling, and causes the tyrosine phosphorylation of multiple proteins. (uncg.edu)
  • showed t hat both forms of train ing had a signi ficant effect on muscu lar streng th and en durance. (researchgate.net)
  • Three other independent Mendelian randomization studies have supported this conclusion. (cdc.gov)
  • It contains descriptions and evaluations of toxicological studies and epidemiological investigations and provides conclusions, where possible, on the relevance of toxicity and toxicokinetic data to public health. (cdc.gov)