Cytochrome c peroxidase, or CCP, is a water-soluble heme-containing enzyme of the peroxidase family that takes reducing equivalents from cytochrome c and reduces hydrogen peroxide to water: CCP + H2O2 + 2 ferrocytochrome c + 2H+ → CCP + 2H2O + 2 ferricytochrome c CCP can be derived from aerobically grown yeast strains and can be isolated in both native and recombinant forms with high yield from Saccharomyces cerevisiae. (wikipedia.org)
Germination of selected vegetable seeds including corn (Zea mays L.), squash (Cucurbita pepo L.), and tomato (Solanum lycopersicum L.) was studied in water with different concentrations of hydrogen peroxide (H2O2) solution ranging from 0, 0. (researchgate.net)
The use of hydrogen peroxide (H2O2) is recently recommended for use in drip irrigation particularly for cleaning of drip emitters. (researchgate.net)
In addition to its peroxidase activity, it acts as a sensor and a signaling molecule to exogenous H2O2, which activates mitochondrial catalase activity. (wikipedia.org)
Exogenous2
Exogenous copper and iron stimulate the ascorbate-induced [Ca2+]cyt. (deepdyve.com)
Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. (deepdyve.com)
Molecules2
In water solutions, this substance is present as a monovalent anion, ascorbate- (Asc-), which easily donates electrons to oxidized molecules and scavenges reactive oxygen species (ROS). (deepdyve.com)
2016) also suggested that H2O2 molecules play dual roles in plant physiological and developmental processes. (researchgate.net)
Antioxidant3
Overall, these are very important physiological roles of AA relating this molecule exclusively to antioxidant functions. (deepdyve.com)
Known dietary antioxidants are vitamins A , C , and E , but the term antioxidant has also been applied to numerous other dietary compounds that only have antioxidant properties in vitro , with little evidence for antioxidant properties in vivo . (wikipedia.org)
This review focuses on the effects of oxidative stress and the role of a particular antioxidant system-the Keap1-Nrf2-ARE pathway-on ocular diseases, specifically age-related macular degeneration, cataracts, diabetic retinopathy, and glaucoma. (hindawi.com)
However, both low-temperature magnetic susceptibility measurements and Mössbauer spectroscopy show that the iron in Compound I of CCP is a +4 ferryl iron, with the second oxidising equivalent existing as a long-lived free-radical on the side-chain of the tryptophan residue (Trp-191). (wikipedia.org)
EPR spectroscopy measurements demonstrate that salinity (NaCl) triggers the accumulation of root apoplastic ascorbyl radicals in an A9C-dependent manner, confirming that l-ascorbate leaks through anion channels under depolarization. (deepdyve.com)
High2
CCP has high sequence identity to the closely related ascorbate peroxidase enzyme. (wikipedia.org)
Based on a novel Ion Powered Pump® (IPP®) delivery system that provides a pharmacokinetic (PK) profile that more closely aligns with the body's own natural sleep pattern, REMfresh has demonstrated once again promising results and high levels of satisfaction in a real-world population of patients who have had chronic difficulties sleeping, providing up to seven hours of sleep support," said Dr. Brodner. (pharmaceuticalintelligence.com)
Water2
showed that the effect of H2O2 on corn seed germination was significantly greater than for seed treated with aerated water but no H2O2. (researchgate.net)
Relatively less known are the effects of H2O2 in irrigation water on seed germination, seedling growth and establishment. (researchgate.net)
Hours1
Compound I of CCP is fairly long-lived, decaying to CCP-compound II with a half-life at room temperature of 40 minutes to a couple hours. (wikipedia.org)
Studies2
The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
PRO studies of this kind, which more closely address real-world patient experience, are increasingly being recognized by regulatory authorities and academia in evaluating new therapies. (pharmaceuticalintelligence.com)
Shows2
The enzyme shows an unusual amino acid pattern compared to other peroxidase. (wikipedia.org)
Intriguingly, the replacement of gluconate with ascorbate in the patch-clamp pipette reveals a large ascorbate efflux current, which shows sensitivity to the anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. (deepdyve.com)
Growth1
tested 24-h seed pre-treatments with H2O2 with 50 mM being optimal with improved seed germination, seedling vigour and growth in corn, squash (Cucurbita pepo L.) and tomato (Solanum lycopersicum L.). Increased germination and seedling growth of peas (Pisum sativum L. 'Alaska') was shown by Barba-Espin et al. (researchgate.net)
Free1
Antioxidants are compounds that inhibit oxidation (usually occurring as autoxidation ), a chemical reaction that can produce free radicals . (wikipedia.org)
Iron1
This mechanism may underlie ascorbate release, signalling phenomena, apoplastic redox reactions, iron acquisition, and control the ionic and electrical equilibrium (together with K+ efflux via GORK channels). (deepdyve.com)