Clostridium thermocellum ATCC 27405 is a candidate for ethanol production from lignocellulosic biomass using consolidated bioprocessing. (biomedcentral.com)
Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems. (pacb.com)
One example is Clostridium thermocellum ATCC 27405T, where genetic tools are not available to help validate decades of hypotheses. (pacb.com)
Plasmid methylation was experimentally validated and successfully electroporated into C. thermocellum ATCC 27405. (pacb.com)
Cellulolyticum3
C. thermocellum, C. cellulolyticum, and C. cellulovorans) have greatly complicated efforts to probe cellulosome structure and function. (wikipedia.org)
In the assembly of the Clostridium cellulolyticum cellulosome, the multiple cohesin modules of the scaffolding protein CipC serve as receptors for cellulolytic enzymes which bear a dockerin module. (rcsb.org)
The nature of the overall surface and of the dimer interface of Cc-cohesin differ notably from those of the Ct-cohesin modules, being much less polar, and this may explain the species specificity observed in the cohesin/dockerin interaction of C. cellulolyticum and C. thermocellum. (rcsb.org)
Bacteria6
Description and significance== Like most species from the Clostridium genus, ''C. thermocellum'' is a bacteria that has a rod-like shape for its cell body. (kenyon.edu)
Cell structure and metabolism== The general interior cellular structure of ''C. thermocellum'' is similar to most rod-shaped bacteria. (kenyon.edu)
Aside from the general internal framework of the cell, ''C. thermocellum'' is a gram-positive bacteria. (kenyon.edu)
The enzyme belongs to the rare group of AroH-type chorismate mutases which are mainly found in gram-positive bacteria of the Bacillus / Clostridia group and have recently also been described for gram-negative organisms. (springer.com)
The researchers explored enzymes from the prodigiously plant-digesting fungus Trichoderma reesei and the cellulose-eating bacteria Clostridium thermocellum . (nsf.gov)
For instance, although anaerobic bacteria, such as Clostridium thermocellum and Acetivibrio cellulalyticus, integrate diverse cellulases, aerobic organisms, such as Trichoderma species and several actinomycetes, generate and exude free lignocellulolytic enzymes (Cells). (pulsus.com)
Cellulosome2
The cellulosome, a complex system of cellulolytic enzymes, is located at the exterior of the ''C. thermocellum'' cell. (kenyon.edu)
24 h) to permit cellulosome production by C. thermocellum . (aimspress.com)
Organism1
C. thermocellum'' is an anaerobic and thermophilic organism that produces spores. (kenyon.edu)
Thermophilic bacterium2
They simply sought a 'cellulose-binding factor' or 'CBF' on the cell surface of the anaerobic thermophilic bacterium, C. thermocellum, which they inferred would account for the observation that the bacterium attaches strongly to the insoluble cellulose substrate prior to its degradation. (wikipedia.org)
Clostridium thermocellum is a thermophilic bacterium recognized for its natural ability to effectively deconstruct cellulosic biomass. (nrel.gov)
Protein3
The celH gene from Clostridium thermocellum encodes a protein containing 900 residues and three components, including Cel5E, Lic26a, and carbohydrate-binding domains. (irost.org)
Because of its ability to break down cellulose, it is highly likely that ''C. thermocellum'' can be isolated from many plant organisms. (kenyon.edu)
Because ''C. thermocellum'' is known as a degrader of cellulose, its DNA contains specific nucleotide sequences that make up the genes that encode for the system of enzymes that are necessary for cellulose degradation. (kenyon.edu)
The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module that adheres cellulose to the cellulosomal complex. (wikipedia.org)
Much of our understanding of its catalytic components, architecture, and mechanisms of attachment to the bacterial cell and to cellulose, has been derived from the study of Clostridium thermocellum. (wikipedia.org)
Cellulolytic3
The cellulolytic phase was initiated by culturing C. thermocellum (63 °C, 24 h). (aimspress.com)
One salient example of this is metabolic flux analysis in industrially relevant cellulolytic microbes, such as Clostridium thermocellum , that metabolize complex substrates (i.e., lignocellulosic biomass). (energy.gov)
The team reports the successful use of deuterated water to investigate the reversibility of glycolytic reactions on three bacterial species of industrial interest: the model bacterium Escherichia coli, the cellulolytic and ethanologenic bacterium C. thermocellum, and the ethanologenic bacterium Zymomonas mobilis , each harboring distinct versions of glycolysis. (energy.gov)
Metabolic1
Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass. (uams.edu)
Escherichia1
Multiple active systems were identified, and corresponding DNA methyltransferases were expressed from the Escherichia coli chromosome to mimic the C. thermocellum methylome. (pacb.com)
Phytofermentans1
The mutated Cphy3925 lost NAD(H)-dependent activities, but, unlike the mutated AdhE in C. thermocellum , the G609D mutation did not result in NADPH-dependent ADH activity (Fig. 3B to E). Instead, our results support the notion that the ET strain arrested AdhE-mediated interconversion of acetyl-CoA, acetaldehyde, and ethanol, which helps explain why the C. phytofermentans ET strain had lower ethanol yield. (researchgate.net)
Extracellular1
Schwarz WH , Zverlov VV , Bahl H . Extracellular glycosyl hydrolases from clostridia. (microbiologyresearch.org)
Plasmid1
In addition to the circular chromosome, ''C. thermocellum'' also has a plasmid which is an extrachromosomal genetic element that is not essential in affecting its lifestyle. (kenyon.edu)
Ethanol production1
This work seeks to inform the deployment of cellulosic ethanol production by furthering our understanding of C.thermocellum mediated deconstruction. (dartmouth.edu)
Deconstruction1
Alternatively, Clostridium thermocellum is a thermophilic anerobe capable of both deconstruction and conversion of lignocellulose without thermochemical pretreatment. (dartmouth.edu)
Chromosome1
The circularity of the chromosome is advantageous for ''C. thermocellum'' because exonucleases cannot digest the ends of its DNA. (kenyon.edu)
Genetic1
This combined approach enabled genetic modification of the C. thermocellum-type strain and acts as a blueprint for transformation of other non-model microorganisms. (pacb.com)
Strain1
A previous study reported an ethanol-tolerant C. thermocellum strain with AdhE mutations (P704L and H735R) that shifted the cofactor specificity from NADH to NADPH, which was proposed to confer ethanol resistance by altering the internal redox balance (9). (researchgate.net)
Transcription1
In regards to genomic organization, ''C. thermocellum'' like all other prokaryotic organisms lack a true nucleus for DNA storage and transcription. (kenyon.edu)
Carbon2
Al-Shorgani NK, Kalil MS, Yusoff W (2011) The effect of different carbon sources on butanol production using Clostridium saccharoperbutylacetonicum N1-4. (aimspress.com)
Carbon from biowaste and Clostridium thermocellum: making second-generation biofuels a reality. (kth.se)
Temperature2
These results indicate that C. thermocellum can survive and produce cellulases with C. saccharoperbutylacetonicum in a continuous-flow, high-solids reactor temperature with temperature cycling. (aimspress.com)
Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacity. (mpg.de)
Production1
Thang V, Kanda K, KobayanshI G (2010) Production of Acetone-Butanol-Ethanol (ABE) in direct fermentation of cassava by Clostridium saccharoperbutylacetonicum N1-4. (aimspress.com)
Study2
The study was conducted to evaluate fermentation by Clostridium thermocellum and C. saccharoperbutylacetonicum in a continuous-flow, high-solids reactor. (aimspress.com)
In addition to Pyrococcus furiosus ( Pfu ), two new organisms, Shewanella oneidensis and Clostridium thermocellum , were added in the study. (secsg.org)
Found1
The same can be done with another thermophilic microbe Bomble works with, Clostridium thermocellum, which can be found anywhere from soil and compost to herbivores and hot springs. (canadianbiomassmagazine.ca)
Present1
Rod-shaped cell body of ''C. thermocellum'']] ==Genome structure== The number of nucleotides present in the genome of ''C. thermocellum'' has been discovered and reported to be at 3,843,301 base pairs which makes up 3307 genes [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genome&cmd=Retrieve&dopt=Overview&list_uids=20502]. (kenyon.edu)
Module1
Despite a rather low sequence identity of 32 %, this module has a fold close to those of the two Clostridium thermocellum cohesin (Ct-cohesin) modules whose 3D structures have been determined previously. (rcsb.org)