Clone hybrid cells. Medical search. Frequent questions

Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping. (nih.gov)
cloning and splicing genes is not its existence, but the results of this unregulated practice. (real-agenda.com)
When I set out to write this article my first challenge was how to present the information in a concise, yet shocking enough to wake up people who still believe that cloning humans for organ harvesting, splicing animal and human genes and making food out of human DNA or tissue is just science fiction. (real-agenda.com)
Cloned human embryos express the genes required for pluripotency, but animal-human hybrids do not, according to a study published today (Feb. 2nd) in the journal__ linkurl:Cloning and Stem Cells. (the-scientist.com)
B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
1. Identification and characterization of Arabidopsis rat mutants and molecular cloning of RAT genes (Gelvin, Citovsky, Hohn, and Ziemienowicz laboratories, with help from undergraduate students at Tuskegee University). (purdue.edu)
The assay is specific for the individual human globin genes and will detect the presence of a globin gene if the relevant chromosome is present in only 10% of the cells of a hybrid population. (eurekamag.com)
But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
Thus, vertebrate HB9/MNR2 genes are expressed specifically in somatic motoneurons and are essential for distinguishing motoneuron/interneuron cell types. (jneurosci.org)
Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2. (nih.gov)

http://www.liebertonline.com/clo __The findings pave the way for isolating human embryonic stem cells from therapeutic cloning -- a landmark that has never been achieved after linkurl:Woo-suk Hwang's discredited cloning experiments;http://www.the-scientist.com/news/display/22933/ -- but call into question the utility of interspecies embryos. (the-scientist.com)
These eggs simply do not reprogram," lead author linkurl:Robert Lanza,;http://www.robertlanza.com/ chief scientific officer of Advanced Cell Technology in Worcester, Mass., said of the human-animal hybrid embryos. (the-scientist.com)
The Howard government in Canberra has no stomach for another exhausting national debate on embryos and reproductive technology, despite the views of an expert review committee which last year unanimously recommended both therapeutic cloning and hybrid embryos. (bioedge.org)
Chinese scientists at various research institutions have reported successful experiments in human cloning, including the production of human-rabbit hybrid embryonic stem cells, according to the claims of Professor Lu Guangxiu at Xiangya Medical College, who told the Wall Street Journal in March of 2002 that researchers at the College had been successfully cloning embryos for two years. (publicintegrity.org)
Japan subsequently enacted legislation in late 2000 criminalizing the cloning of human embryos for reproductive purposes. (publicintegrity.org)
China enacted regulations early this year to allow the cloning of human embryos for research, and South Korea enacted similar legislation to allow research days ahead of the February announcement. (publicintegrity.org)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
The Catholic Church has always held that stem-cell research and therapies are morally acceptable, as long as they don't involve the creation and destruction of human embryos. (archstl.org)

If you cannot or do not want to get into the heavy research, I am about to give you a detailed report on the state of genetic engineering, human-animal cloning and gene splicing. (real-agenda.com)
Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids showed that the human gene for D2H resides on chromosome 2. (jci.org)
Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
We have successfully used a DNA.cDNA molecular hybridization assay to directly determine the presence or absence of human beta globin gene sequences in 20 human-mouse somatic cell hybrids, each of which contained a different subset of human chromosomes. (eurekamag.com)
All human chromosomes were present in one or more cell lines devoid of the human beta globin gene except for 6, 8, 9, 11, and 13. (eurekamag.com)
Among these latter chromosomes, only chromosome 11 was present in the six hybrid clones that contained the human beta globin gene. (eurekamag.com)
In fact, chromosome 11 was the only human chromosome that was present in all of the six hybrid clones found to be positive for the human beta globin gene. (eurekamag.com)
Pcp2 was first identified as a candidate gene to explain Purkinje cell degeneration in pcd mice (Nordquist, D. T., Kozak, C. A., and Orr, H. T. (1988) J. Neurosci. (embl-heidelberg.de)
Amazingly, the first human cancer gene was cloned only thirty years ago. (jcancer.org)
Using polymerase chain reaction amplification of human-specific products from human x rodent somatic cell hybrid DNAs, the gene has been assigned to human chromosome 6. (aspetjournals.org)
On the basis of recent vertebrate studies, the HB9/MNR2 gene family is a prime candidate for a general determinant of somatic motoneuron cell type. (jneurosci.org)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)
Important examples of such mutations include the RHD pseudogene and RHD-CE-D hybrid gene. (medscape.com)
As of first of January 2022, StemTherapy and MultiPark have decided to merge the former iPSC, CRISPR and vector platforms into the new Cell and Gene Therapy Core . (lu.se)
The Cell and Gene Therapy core is an open-access infrastructure and our services include AAV and LV vector production, cloning services, iPS reprogramming, iPS-edits and CRISPR experimental designs. (lu.se)

Two commonly discussed types of human cloning are therapeutic cloning and reproductive cloning. (wikipedia.org)
Therapeutic cloning would involve cloning cells from a human for use in medicine and transplants. (wikipedia.org)
Two common methods of therapeutic cloning that are being researched are somatic-cell nuclear transfer and (more recently) pluripotent stem cell induction. (wikipedia.org)
these are the "holy grail" that would be useful for therapeutic or reproductive cloning. (wikipedia.org)
At last weekend's council of Australian governments, the Federal Government stoutly resisted pressure to lift a ban on therapeutic cloning, along with the largest state, New South Wales. (bioedge.org)
However, the premiers of Victoria and Queensland have become fervent believers in therapeutic cloning as a magic bullet for voters' health, scientists' jobs and government finances. (bioedge.org)
In the week before the meeting, two of Australia's most distinguished scientists gave strong backing to therapeutic cloning. (bioedge.org)
A growing number of U.S. legislators seem prepared to support research on therapeutic cloning. (publicintegrity.org)
But adult stem cells also raise some interesting ethical dilemmas alongside their great therapeutic promise. (eppc.org)
Antibodies directed at epitopes unique to or overexpressed on the cancer cells could be of therapeutic utility. (ias.ac.in)
John Bryant discusses his own views on the ethical dangers of using embryonic cells for therapeutic uses. (testoffaith.com)

What do you think about animal-human hybrids? (testoffaith.com)
An explanation of the technology of using animal-human hybrids to make embryonic stem cells. (testoffaith.com)

With the cloning of a sheep known as Dolly in 1996 by somatic cell nuclear transfer (SCNT), the idea of human cloning became a hot debate topic. (wikipedia.org)
It became a hot topic in 1996 when Dolly the sheep was cloned via a process called somatic cell nuclear transfer. (archstl.org)

Cells that secrete antibodies can be made immortal by fusing them with tumor cells and cloning the hybrids. (scientificamerican.com)

The Western blot is a technique used to identify candidate hybrids, clones, subclones and monoclonal antibodies of interest that recognize and bind to one or more specific molecular weight proteins of interest. (mayo.edu)
Monoclonal antibodies derived from ten hybrid cell clones, generated against porcine zona pellucida gave strong immunofluorescence with zona but the pattern varied from patchy, thin rim to heavy precipitation type of rim. (ias.ac.in)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)

I would also add that it is important we do not lose sight of the fact that while in theory embryonic stem cell research holds promise for some hope in treating maladies, nothing has been proven. (christianliferesources.com)
A decade later, cloning came to the forefront in Missouri with the narrow passage of Amendment 2, a ballot initiative in 2006 that constitutionally protects embryonic stem-cell research and human cloning. (archstl.org)

In 2004 and 2005, Hwang Woo-suk, a professor at Seoul National University, published two separate articles in the journal Science claiming to have successfully harvested pluripotent, embryonic stem cells from a cloned human blastocyst using SCNT techniques. (wikipedia.org)
In addition, the possibility of reprogramming adult stem cells back to a "pluripotent" (or embryonic-like) state raises the biological prospect of going back too far. (eppc.org)
Embryonic stem cells are pluripotent - they have the ability to become virtually any type of cell within the body. (cbc.ca)

Cloning and chromosomal localization of a human kidney cDNA involved in cystine, dibasic, and neutral amino acid transport. (jci.org)
We have recently cloned, sequenced, and characterized a rat kidney cDNA (D2) that stimulates cystine as well as dibasic and neutral amino acid transport. (jci.org)
In order to evaluate the role of this protein in human inherited diseases such as cystinuria, we have isolated a human D2 clone (D2H) by low stringency screening of a human kidney cDNA library using the radiolabeled D2 insert as a probe. (jci.org)
To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (nih.gov)
We have isolated cDNA clones for ovine α -subunit and LHβ. (ias.ac.in)
A cDNA encoding the human gamma-aminobutyric acid (GABA)A receptor beta 2 subunit has been cloned and sequenced. (aspetjournals.org)

a nucleus was taken from a man's leg cell and inserted into a cow's egg from which the nucleus had been removed, and the hybrid cell was cultured and developed into an embryo. (wikipedia.org)
In this case, each embryo was created by taking a nucleus from a skin cell (donated by Wood and a colleague) and inserting it into a human egg from which the nucleus had been removed. (wikipedia.org)
I have been asked to comment on the latest news that scientists are now able to harvest embryonic stem cells without killing the embryo. (christianliferesources.com)
Specifically, two new procedures are being reported in an effort to avoid the ethical offense of killing the embryo when extracting stem cells. (christianliferesources.com)
It involves extracting a cell from an embryo and then stimulating that cell to produce stem cells. (christianliferesources.com)
Several western scientists have been conducting their research in Asian countries in the past few years, including Cibelli, formerly of Advanced Cell Technology, an early U.S. pioneer of embryo research, as well as Alan Colman, now located in Singapore, one of the scientists who helped create the first mammalian clone, the sheep Dolly. (publicintegrity.org)
Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)
After differentiation, fully developed cereal endosperm makes up to 75% of the grain weight and covers four major cell types: aleurone, starchy endosperm, transfer cells, and the cells of the embryo surrounding region 1 . (nature.com)
That's why Father Pacholczyk, director of education at the National Catholic Bioethics Center in Philadelphia, said that the efforts to help people understand the immorality of embryo reserch, including human cloning, must focus on humanizing the issue and appreciating our own embryonic origins, not just on the desired results of embryonic or other types of stem-cell research. (archstl.org)

My question regarding genetic engineering deregulation was then: What would happen if scientists who are provided with unlimited money and resources have no legal liability to realize their experiments cloning humans and literally engineering new species? (real-agenda.com)
Yeast one-hybrid (Y1H) experiments suggested that LsARF3 could physically interact with the LsCO promoter, which was further confirmed by a dual luciferase assay in tobacco leaves. (frontiersin.org)
Many of these people testify to experiments done on their genitals, including the removal of sperm, some testify that they have had "alien creatures" taken from their womb by these "Aliens", and/or to being shown human/alien hybrid children. (exposingsatanism.org)
After years of experiments …cloning hit the big time in February 1997. (exposingsatanism.org)
The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)

Although the possibility of cloning humans had been the subject of speculation for much of the 20th century, scientists and policymakers began to take the prospect seriously in 1969. (wikipedia.org)
Many nations outlawed it, while a few scientists promised to make a clone within the next few years. (wikipedia.org)
In 2011, scientists at the New York Stem Cell Foundation announced that they had succeeded in generating embryonic stem cell lines, but their process involved leaving the oocyte's nucleus in place, resulting in triploid cells, which would not be useful for cloning. (wikipedia.org)
In 2013, a group of scientists led by Shoukhrat Mitalipov published the first report of embryonic stem cells created using SCNT. (wikipedia.org)
Should scientists seek to clone our ancient hominid cousins? (nationalgeographic.com)
Since the 1996 birth of Dolly the sheep, the world's first cloned mammal, scientists have greatly expanded and improved on cloning techniques. (nationalgeographic.com)
Starting with an intact cell (fresh or frozen) of the animal they'd like to clone, scientists first remove the nucleus, where DNA resides, and insert it into a hollowed-out egg cell of the same or a related species. (nationalgeographic.com)
In Japan, scientists at Kyoto University announced in January that they had successfully produced embryonic stem cells domestically for the first time. (publicintegrity.org)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)
But they are also less equipped to produce every cell type of the body and less able to reproduce themselves indefinitely, which makes them less appealing to scientists interested in basic research. (eppc.org)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
My point is, that we have no idea how long scientists/occultists around the world have been creating hybrids/chimeras and injecting them into society. (exposingsatanism.org)
That month, scientists reported the first successful attempt to reproduce a large, adult mammal through cloning. (exposingsatanism.org)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
The Cellomics Arrayscan VTI instrument is an imaging tool that will help our scientists make quicker and more precise analyses, capturing, for instance, changes in cell size, shape and intensity. (lu.se)

With that publication, "genome engineering of mammalian cells just took a big step forward," he says. (nationalgeographic.com)
Mammalian Purkinje-cell protein-2 (Pcp2). (embl-heidelberg.de)

Reproductive cloning would involve making an entire cloned human, instead of just specific cells or tissues. (wikipedia.org)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)
However, they are hampered by the EU Tissues and Cells Directive, which was introduced to stop the international black market in human organs, and which bans financial incentives for donors. (hgalert.org)
Tissues and Cells. (lu.se)

In nature, hybrids and chimeras are rare due to incompatibilities and developmental divergence during evolution. (frontiersin.org)
How evolutionary distance affects the formation of hybrids and chimeras is an open question that may vary from situation to situation. (frontiersin.org)

MoBiTec is dedicated to developing, producing and marketing unique tools for molecular and cell biology. (hum-molgen.org)
MoBiTec remains committed to providing an extraordinary wide range of high-quality, innovative products for state-of-the-art molecular and cell biology backed by an unsurpassed support team. (hum-molgen.org)
We are prepared to manage, distribute and service additional products in the field of molecular and cell biology and related fields. (hum-molgen.org)

In ancient history, humans used the term "chimera" to describe mythical creatures and hybrids. (frontiersin.org)
Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)
Biologist and bioethicist John Bryant explains the status of the technology of 'cloning' living organisms, and humans. (testoffaith.com)

Four embryonic stem cell lines from human fetal somatic cells were derived from those blastocysts. (wikipedia.org)

Nobel Prize-winning geneticist Joshua Lederberg advocated cloning and genetic engineering in an article in The American Naturalist in 1966 and again, the following year, in The Washington Post. (wikipedia.org)
Using new tricks of genetic engineering, researchers could make adjustments to the DNA in the human cell so it matches the code of the Neanderthal. (nationalgeographic.com)
The flow of genetic information in cells is which of the following? (proprofs.com)
The flow of genetic information in cells is that DNA is first transcribed into RNA through a process called transcription. (proprofs.com)

In this experiment, the researchers developed a protocol for using SCNT in human cells, which differs slightly from the one used in other organisms. (wikipedia.org)
Animals sexually derived from the fusion of gametes from two different organisms, such as mules, are considered "hybrids. (frontiersin.org)
On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)

The United States currently has no comprehensive law, and legislation that would have banned both research and reproductive cloning has failed to reach a vote in the Senate after approval in the House of Representatives in July 2001. (publicintegrity.org)

The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)

16 Selection for the active X chromosome yielded 12 clones bearing only the normal X and five clones retaining both X chromosomes. (bmj.com)
Back selection for the inactive X on one diploid clone yielded 12 hybrids containing the deleted inactive X chromosome, three of which were selected for analysis. (bmj.com)
Chromosome painting, performed on metaphase chromosomes preparations with an X chromosome specific probe (P5222-DG.5, Oncor), 17 confirmed that these hybrids contained only one X chromosome. (bmj.com)
A long range deletion map was constructed using available X chromosome YAC contigs and the DNA of the proband of family 1, hybrid 1C3 derived from the mother of family 2, and patients 3 and 4. (bmj.com)

Another Nobel Laureate, James D. Watson, publicized the potential and the perils of cloning in his Atlantic Monthly essay, "Moving Toward the Clonal Man", in 1971. (wikipedia.org)
These moral perils are surely not a reason to oppose adult stem cell research, which deserves vigorous and expanded public support. (eppc.org)

In his 2012 book Regenesis , Harvard geneticist George Church proposes a different approach for cloning extinct animals whose genome has been sequenced. (nationalgeographic.com)
We know from cloning experience that there's a very high failure rate," says geneticist James Noonan of Yale University. (nationalgeographic.com)

The first hybrid human clone was created in November 1998, by Advanced Cell Technology. (wikipedia.org)
Cloning is as much an art as it is a science," said Robert Lanza of Advanced Cell Technology in Worcester, Massachusetts. (exposingsatanism.org)

In contrast, stem cells extracted from adults and umbilical cord blood have both proven very promising without placing lives at risk. (christianliferesources.com)
The Church also supports research and therapies using adult stem cells, which are cells that come from any person who has been born - including umbilical cord blood, bone marrow, skin and other organs. (archstl.org)

A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)

Although stem cell research efforts have been in place for several years, the top countries in Asia have only recently begun to regulate the science. (publicintegrity.org)
While an international framework to regulate cloning remains stalled in the United Nations, some Asian countries are offering more stable climates for researchers to pursue their work. (publicintegrity.org)
Ca 2+ channel β subunits regulate cell-surface expression and gating of voltage-dependent Ca 2+ channel α1 subunits. (jneurosci.org)

L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) are coupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. (embl-heidelberg.de)
It may function as a cell-type specific modulator for G protein-mediated cell signalling. (embl-heidelberg.de)
Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. (embl-heidelberg.de)
Pcp2 (Purkinje cell protein-2) was identified as a partner for Galphao in this system. (embl-heidelberg.de)

Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. (cdc.gov)

Over the past few years, the debate over stem cells and cloning has grown both more complex and more profound. (eppc.org)
But there has been intense debate over the use of stem cells. (cbc.ca)

The Antibody Hybridoma Core utilizes the fusion partner cell line FOX-NY, a nonimmunoglobulin-secreting myeloma cell line that is hypoxanthine-aminopterin-thymidine (HAT) sensitive. (mayo.edu)
The Antibody Hybridoma Core offers hybridoma, clone and subclone supernate screening by enzyme-linked immunosorbent assay (ELISA), Western blot assay and fluorescence-activated cell sorting (FACS). (mayo.edu)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)

Each clone is a long-term source of substantial quantities of a single highly specific antibody. (scientificamerican.com)
T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
Nine to 14 days after the fusion, hybrid supernatants are tested for the presence of the specific antibody of interest. (mayo.edu)
A monoclonal antibody (Moab) 2C4 has been generated, which binds with cells of two androgenindependent prostate cancers, DU145 and PC3, and does not bind to peripheral blood leukocytes (PBLs) of healthy donors. (ias.ac.in)
This antibody, along with the previously developed Moab 730, kills 100% of both DU145 and PC3 cells in the presence of complement and does not have a deleterious effect on PBLs of healthy males. (ias.ac.in)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)

This would have been the first major breakthrough in human cloning. (wikipedia.org)
In December 1999, the editors of Science, the journal devoted to scientific and medical matters, called stem cell research the 'Breakthrough of the Year. (cbc.ca)
CAR T cell therapy, the breakthrough method of supercharging patients' T cells in the lab to attack cancer when re-infused, has worked more effectively against cancers of the blood than solid tumors. (medicalxpress.com)

P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)

And recent experimental trials, while still very preliminary, suggest that adult stem cells may one day help us treat a host of terrible pathologies. (eppc.org)
Yet for now, the utility of adult stem cells also remains limited. (eppc.org)
Adult stem cells are easier to control than embryonic stem cells and thus less likely to form tumors. (eppc.org)
Stem cells come in three forms: embryonic stem cells, embryonic germ cells and adult stem cells. (cbc.ca)
Adult stem cells are more limited. (cbc.ca)

In his speech on "Biological Possibilities for the Human Species of the Next Ten Thousand Years" at the Ciba Foundation Symposium on Man and his Future in 1963, he said: It is extremely hopeful that some human cell lines can be grown on a medium of precisely known chemical composition. (wikipedia.org)
The first "immortal" human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. (who.int)

Medical Xpress) -- One of cancer's greatest and most insidious threats is metastasis the three-dimensional migratory invasion of cancer cells from primary tumors to a distant part of the body. (medicalxpress.com)

These toxins bind to specific receptors of the intestinal epithelial cells and cause secretion of water and electrolytes into the intestinal lumen. (cdc.gov)
The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)
By expressing recombinant human GABAA receptors containing different beta subunits (beta 1, beta 2 or beta 3) in both transfected cells and Xenopus oocytes, we have been able to determine the influence of the beta subunit on the pharmacology of the receptor. (aspetjournals.org)

Continuing production and specificity are obtained by fusing the agent to a tumour cell to produce a hybrid cell which retains the characteristics of both partners. (ato.gov.au)
The anti-tumour action of the two antibodies prevents the establishment of DU145 cell tumour in nude mice in vivo. (ias.ac.in)

q23.1 and 14q32.2 by radiation hybrid mapping. (nih.gov)

Even if a clone did survive, the ethical dilemmas of raising a Neanderthal would be complicated. (nationalgeographic.com)

The fusion process involves fusing spleen or lymph node cells with a myeloma fusion partner cell line. (mayo.edu)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
That process requires a living cell," evolutionary biologist Beth Shapiro of University of California, Santa Cruz told NPR when talk of resurrecting the mammoth gained new currency in 2015. (kunr.org)

It is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons. (embl-heidelberg.de)
Pcp2 is expressed in cerebellar Purkinje cells and retinal bipolar neurons, two locations where Galphao is also expressed. (embl-heidelberg.de)

Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

Flow cytometric analysis of Akt (pS473) expression in human Jurkat cells. (bdbiosciences.com)
The data demonstrate that the level of phosphorylation of Akt (pS473) decreases when phosphatidylinositol 3-kinase activity is inhibited by the treatment of Jurkat cells with Wortmannin. (bdbiosciences.com)

In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)

The privately-funded experiment, which took place at Seoul National University under the guidance of Korean Hwang Woo-suk and American Jose Cibelli, was only the latest in a group of announcements from research institutions in Asia in the last few years, and demonstrates that cloning research is becoming "globalized" like any other commodity. (publicintegrity.org)

CAR T cell immunotherapy for solid tumors? (medicalxpress.com)

Anatomy22

Organisms5

Diseases8

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes34

Information Science3

Genes10

Embryos15

Gene15

Therapeutic11

Animal-human hybrids2

Somatic cell nuc2

Tumor cells1

Monoclonal Antibodies4

Embryonic stem cel2

Pluripotent3

CDNA6

Embryo10

Experiments5

Scientists17

Mammalian2

Tissues4

Chimeras2

Molecular3

Humans3

Fetal1

Genetic4

Organisms3

Reproductive1

Epitopes1

Chromosome4

Perils2

Geneticist2

Advanced Cell Techn2

Umbilical cord2

Vitro1

Regulate3

Protein6

Characterize1

Debate2

Hybridoma3

Antibody5

Chimera1

Breakthrough3

Populations1

Adult stem5

Grown2

Primary tumors1

Receptors3

Tumour2

Radiation hybrid1

Ethical dilemmas1

Myeloma1

Evolutionary2

Neurons2

Induction1

Jurkat2

Stem cells from adult1

Years1

Tumors1