Epstein-BarrAntibodies2021InfectionTransplantation antigensProteinProteinsHepatitisCellsPeptideHumoralAntibodyImmuneInfectionsKineticsGenomeReceptorDiagnosisCytotoxicMacrophagesEpitopeSensitivityAssayTissuesSimultaneousBacteriaPresentationClinicalRapidAdaptiveEnvelopeReceptorsRespiratoryDetectionIsolationSuccessfullyDoseTest
Epstein-Barr3
- Using these data, we determined the heritability and genomic loci associated with antibody epitope selection, response breadth, and control of Epstein-Barr virus (EBV) viral load. (kcl.ac.uk)
- 107 EBV peptide reactivities were heritable and at least two Epstein-Barr nuclear antigen 2 (EBNA-2) reactivities were associated with variants in the MHC class II locus. (kcl.ac.uk)
- RC5: data virus de Epstein Barr y se identificó el genoma viral en el líquido cefalorraquídeo, a través de la prueba de reacción en cadena management. (bvsalud.org)
Antibodies8
- Detection of African swine fever viral antigens in paraffin-embedded tissues by use of immunohistologic methods and polyclonal antibodies. (google.es)
- Fourth generation tests for the diagnostics of HIV infection simultaneously detect the presence of HIV antibodies and p24 antigen. (nel.edu)
- Moreover, the antigenic diversity of these new recombinant enzymes when measured with different anti-virus antibodies has also been largely reduced, indicating a better presentation of the epitopes located in the viral peptide. (uab.cat)
- In addition, the licensed screening tests, which use HTLV-I antigens, vary in their sensitivity to detect antibodies to HTLV-II (4,5). (cdc.gov)
- Here we report a method for the characterization of protein internalization in real time through the sortase-mediated, site-specific labeling of single domain antibodies or viral proteins with a newly developed, cathepsin-sensitive quenched-fluorophore probe. (aacrjournals.org)
- Indirect immunofluorescence assays (IFAs) using two epitope-specific monoclonal antibodies (MAbs) were used to examine the expression kinetics of two potentially immunogenic and diagnostically relevant viral antigens, an envelope glycoprotein and a capsid-associated protein. (stir.ac.uk)
- SPIs are made out of a strong help on which antibodies or aptamers, well defined for viral antigens, have been immobilized. (alliedacademies.org)
- While seasonal outbreaks are associated with mutation of the haemagglutinin (HA) protein on the viral surface to escape neutralization by antibodies generated in previous exposures, pandemics result from the introduction of completely new viruses into populations, where there is little pre-existing immunity to that virus 2 . (nature.com)
20212
- In 2016, the SEAR Immunization Technical Advisory Group endorsed a regional hepatitis B control goal with a target of achieving hepatitis B surface antigen (HBsAg) seroprevalence of ≤1% among children aged ≥5 years by 2020, which is in line with the WHO Global Health Sector Strategy on Viral Hepatitis 2016-2021 ( 2 , 3 ). (cdc.gov)
- Head-to-head evaluation of Roche SARS-CoV-2 Rapid Antigen Test and real-time reverse transcription polymerase chain reaction (RT-PCR) as SARS-CoV-2 screening tools performed in asymptomatic adults from a semi-closed community in University of Navarra (Spain) from November 2020 to January 2021. (cun.es)
Infection8
- Herein, the authors focus on recent advances in the understanding of ncRNAs in EV infection from basic viral pathogenesis to clinical prospects, providing a reference basis and new ideas for disease prevention and research directions. (duke.edu)
- Daily percentage of positive SARS-CoV-2 tests (lines) and 95% CIs (shaded areas) of RT-PCR tests, home antigen tests, and viral culture among 225 participants with RT-PCR-confirmed SARS-CoV-2 infection. (jamanetwork.com)
- Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection. (jamanetwork.com)
- This reduction of T cell activity may result in a more potent induction of neutralizing antibody responses, clearing the viral infection. (usda.gov)
- The complex course of chronic HBV infection is associated with the age/route of viral acquisition, host factors such as immune and endocrine factors, viral factors, and host-viral interactions. (mendeley.com)
- The rate of expression of these antigens was determined following a time-course of infection in two CyHV-3 susceptible cell lines. (stir.ac.uk)
- This activity most likely results from the strong induction of interkeukin-12 (IL-12) and gamma interferon (IFN-{gamma}), which are also essential factors in the establishment of protective immunity against viral infection. (usu.edu)
- We tested for HCV antibody and HCV core antigen as markers for exposure to HCV and active infection, respectively. (who.int)
Transplantation antigens4
- Analysis of transplantation antigens in friend viral leukemia. (jax.org)
- The Thy19.4 gene contains an open reading frame, and in several aspects resembles the genes that encode the transplantation antigens. (caltech.edu)
- However, unlike the classical transplantation antigens, the Thy19.4 gene maps distal to the H-2 region, in the Hmt region. (caltech.edu)
- Some of the expressed genes, like the T18ᶜ and Thy19.4 genes, appear to be able to encode class I molecules which may share structural characteristics with the classical transplantation antigens and may possibly serve as recognition structures in cell-cell interaction events. (caltech.edu)
Protein8
- The TAP1 and TAP2 proteins attach (bind) together to form a protein complex called transporter associated with antigen processing (TAP) complex. (medlineplus.gov)
- Detection of protein homolougs with HIV-1 antigens in bacteria of positive patients - phase II. (nel.edu)
- Hainova K, Adamcikova Z, Ciernikova S, Stevurkova V, Krčméry V, Zajac V. Detection of protein homolougs with HIV-1 antigens in bacteria of positive patients - phase II. (nel.edu)
- Feliu, JX, Carbonell, X & Villaverde, A 2000, ' Successful mimicry of a complex viral antigen by multiple peptide insertions in a carrier protein ', FEBS Letters , vol. 474, pp. 87-92. (uab.cat)
- First, the viral N protein was used as a model antigen to validate the assay. (unl.edu)
- Glycoprotein (GP) is the major protective antigen of EBOV, and can generate virus-like particles (VLPs) by co-expression with matrix protein (VP40). (frontiersin.org)
- In this study, we constructed a recombinant Alphavirus Semliki Forest virus (SFV) replicon vector DREP to express EBOV GP and matrix viral protein (VP40). (frontiersin.org)
- A protein antigen from an Eimeria protozoan has recently been reported to induce antitumor activity in mice. (usu.edu)
Proteins3
- Also sold under the brand names HAVpur and VIROHEP-A. This vaccine consists of virosomes, artificial particles composed of synthetic lipids and influenza proteins in addition to the hepatitis A antigen. (wikipedia.org)
- By increasing the number of viral peptides per enzyme monomer, the average IC50 of hybrid proteins in a competitive enzyme-linked immunosorbent assay) have decreased to values close to that presented by natural virions. (uab.cat)
- Their principal mode of action is that they trigger the synthesis of one or more cellular proteins that inhibit viral replication. (nursingtermpaper.com)
Hepatitis4
- The definition of (U)nits varies among manufacturers depending on how hepatitis A antigen is measured in their products. (wikipedia.org)
- Most chronic HBV infections, indicated by the presence of hepatitis B surface antigen (HBsAg) on serologic testing, are acquired in infancy through perinatal or early childhood transmission ( 2 ). (cdc.gov)
- Majority of chronic HBV infected children present with immune-tolerant status initially, experience the immune clearance phase with various degree of liver injury during or beyond puberty, and then enter the inactive phase after hepatitis B e antigen (HBeAg) seroconversion. (mendeley.com)
- Objective: The World Health Organization's guidelines on viral hepatitis testing and treatment recommend prioritizing high prevalence groups. (who.int)
Cells9
- We attempted viral culture at a CDC laboratory for all participants testing positive by RT-PCR or antigen test by using Vero-CCL81 cells, which were inoculated with clinical specimens, and observed daily for 7 days ( 8 ). (cdc.gov)
- A Molecular Basis for the Interplay between T Cells, Viral Mutants, and Human Leukocyte Antigen Micropolymorphism. (expasy.org)
- Acute to subacute tubulointerstitial nephritis and necrotizing pancreatitis were the most frequent histologic lesions, with immunolabeling of viral antigen in renal tubular epithelial cells and pancreatic acinar epithelium. (usgs.gov)
- Peritoneal exudate cells recovered from mice injected intraperitoneally with ad-vIL-10 inhibited DTH reactions in recipient mice, but only when the donor mice had been sensitized to the antigen used to incite the DTH response. (elsevierpure.com)
- Interleukin-10 (IL-10) suppresses cellular immune response by modulating the function of T cells and antigen-presenting cells. (usda.gov)
- Proinflammatory islet antigen reactive CD4 T cells are linked with response to alefacept in type 1 diabetes. (immunetolerance.org)
- Here we develop a BAFF ligand-based chimeric antigen receptor (CAR) and generate BAFF CAR-T cells using a non-viral gene delivery method. (lonza.com)
- Second, the adjuvant is able to increase the adaptability of the recalled cells to give improved specificity to the new vaccine antigen. (nature.com)
- We identified an EBV serosignature that predicted viral load in peripheral blood mononuclear cells and was associated with variants in the MHC class I locus. (kcl.ac.uk)
Peptide3
- The c-Myc peptide sequence was chosen to model a viral epitope. (smith.edu)
- The antigenic properties of a viral peptide from the surface of foot-and-mouth disease virus particles have been successfully mimicked by multiple insertion in solvent-exposed regions of Escherichia coli β-galactosidase. (uab.cat)
- Although bivalent antibody binding could have been favoured by multiple presentation, conformational modifications of the viral peptide, due to the presence of other insertions or a cooperative antibody binding cannot be excluded. (uab.cat)
Humoral2
- These findings help to explain the utility of certain single domain antibody-antigen conjugates for inducing humoral immune responses. (aacrjournals.org)
- Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)
Antibody3
- Notably, vaccination with DREP-GP and DREP-VP40, which produces both GP and VP40 antigens, induced a significantly higher level of anti-GP IgG2a antibody and increased IFN-γ secreting CD8 + T-cell responses relative to vaccination with DREP-GP or DREP-VP40 vector alone. (frontiersin.org)
- Explain why a secondary antibody response to an antigen may prevent a bacterial or viral disease when the primary adaptive immune response to that antigen did not protect the person from the disease. (nursingtermpaper.com)
- We used VirScan, a high-throughput epitope scanning technology, to analyze pan-viral antibody reactivity profiles of twins and SNP-genotyped individuals. (kcl.ac.uk)
Immune6
- Researchers are unsure why people with BLS I do not also get viral infections, but they suspect that other immune processes are able to recognize and fight viruses. (medlineplus.gov)
- However, it is unclear what function these molecules play, or why such a large gene family is maintained if only three viral antigen-presenting restriction elements are required by the murine immune system. (caltech.edu)
- The genotype and phenotype of human cytokines, innate immunity, and human leukocyte antigens are also associated with the onset of immune clearance of HBV and severity of inflammation. (mendeley.com)
- Immune escape HBV mutant strains, emerged during the immune clearance phase under host immune surveillance, may cause different impacts on viral biosynthesis, host immune responses, and clinical course. (mendeley.com)
- AS03 and MF59 enhance innate immune responses by increasing antigen uptake and presentation in the local tissue. (nature.com)
- Human immune responses to viral infections are highly variable, but the genetic factors that contribute to this variability are not well characterized. (kcl.ac.uk)
Infections4
- Therefore, a comprehensive examination of antigen test performance characteristics in identifying infectious persons who have SARS-CoV-2 infections requires comparison with multiple data points, including RT-PCR test positivity and the ability to isolate the virus (a marker for infectiousness) ( 5 - 7 ). (cdc.gov)
- Interferons are an important part of the host defense against viral infections. (nursingtermpaper.com)
- Moreover, because of their low speed, moderately significant expense, and need for prepared faculty, these methodologies actually can't distinguish about 33% of respiratory viral infections, viral gastroenteritis, and viral encephalitis [ 3 ]. (alliedacademies.org)
- These advances make a change in perspective in the determination of viral contaminations by exploiting scaling down, robotization, reasonableness, and cost-viability to foster high-throughput screening stages that completely distinguish the tremendous variety of mammalian infections known to taint human [ 4 ]. (alliedacademies.org)
Kinetics1
- 2015, 'Simultaneous quantification of viral antigen expression kinetics using data-independent (DIA) mass spectrometry', Molecular and Cellular Proteomics, vol. 14, no. 5, pp. 1361-1372. (edu.au)
Genome2
- In addition to the genes that encode the three classical class I antigens, the BALB/c genome contains 32 genes that structurally resemble the classical class I genes, and therefore possibly encode class I molecules. (caltech.edu)
- Upon the host cell acknowledgment, the viral envelope wires with the host cell layer permitting the viral genome to taint the host [ 2 ]. (alliedacademies.org)
Receptor1
- CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation. (biolegend.com)
Diagnosis2
- The Infectious Diseases Society of America guidelines on the diagnosis of COVID-19: antigen testing. (jamanetwork.com)
- The control brain tomography showed persistent ventriculomegaly and cerebral edema, which led to RG, Caltagirome R, Blanco the diagnosis of encephalitis of viral etiology complicated by epilepsy secondary to a demyelinating structural lesion of Pimiento EC, et al. (bvsalud.org)
Cytotoxic1
- In the BALB/c mouse, there are three classical class I molecules, H-2Kᵈ, Dᵈ, and Lᵈ, which are expressed throughout the body and present viral antigens to cytotoxic T lymphocytes (CTLs). (caltech.edu)
Macrophages1
- Collectively, these data suggest a mechanism for the contralateral effect in which genetically modified macrophages and DCs present antigen in the context of high, local concentrations of vIL-10, thereby generating unresponsive T lymphocytes. (elsevierpure.com)
Epitope2
- The proposed immunotherapy will consist of two components: a tumor-targeting agent and a viral epitope for which a patient has active immunity. (smith.edu)
- This thesis aims to characterize conjugation methodologies between a tumor-targeting agent and a model viral epitope to assess feasibility of developing this novel therapeutic strategy. (smith.edu)
Sensitivity3
- Sensitivity (dots) and 95% CIs (error bars) of home antigen tests compared with 3 reference standards: a positive reverse transcription-polymerase chain reaction (RT-PCR) test at any time during the investigation, a positive RT-PCR test collected the same day as the antigen test, and a positive viral culture collected the same day as the antigen test. (jamanetwork.com)
- For participants younger than 12 years, the 95% CI for antigen test sensitivity compared with a positive same-day viral culture was calculated without cluster adjustment as all tests were contributed by unique participants. (jamanetwork.com)
- Test sensitivity was related to viral load, with higher sensitivity in RT-PCR cycle threshold (Ct) values under 25 (93·75%, CI 95%: 71·96 - 98·93), that dropped to 29·41% (CI 95%: 10·31- 55·96) in RT-PCR Ct values above 25. (cun.es)
Assay1
- Lambert-Niclot S , Cuffel A , Pape L , Vauloup-Fellous C , Morand-Joubert L. Evaluation of a rapid diagnostic assay for detection of SARS-CoV-2 antigen in nasopharyngeal swabs. (ajtmh.org)
Tissues1
- The polypeptide component of telomerase (TERT) is an attractive candidate for a broadly expressed tumor rejection antigen because telomerase is silent in normal tissues but is reactivated in more than 85% of cancers. (nova.edu)
Simultaneous1
- Of 2 simultaneous swab specimens, we used 1 specimen for rapid antigen testing by the Abbott BinaxNOW SARS-CoV-2 Antigen Card Test, a point-of-care lateral flow test with results available within 15 minutes of specimen collection. (cdc.gov)
Bacteria1
Presentation1
- Additionally, we described the interference of FMDV with DC maturation and antigen presentation capacity. (usda.gov)
Clinical3
- We placed the other specimen in viral transport medium and transported it on ice to the Marshfield Clinical Research Institute laboratory (Marshfield, Wisconsin, USA) for RT-PCR testing. (cdc.gov)
- Further studies are needed to evaluate different options to improve screenings based on rapid antigen test, such as the use of clinical questionnaires to select higher risk-participants, the confirmation of negative results with RT-PCR or the use of repetitive sequential testing. (cun.es)
- Clinical conclusion of viral diseases depends on identifying viral atoms (oligonucleotides or glycoproteins) in natural media. (alliedacademies.org)
Rapid5
- Antigen platforms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic testing have rapid turnaround time, are easy to use, and are less expensive than real-time reverse transcription PCR (RT-PCR) diagnostic testing. (cdc.gov)
- Comparison of cycle threshold (Ct) values among symptomatic and asymptomatic study participants and across rapid antigen tests. (ajtmh.org)
- however, the median Ct values were significantly higher in individuals with false-negative rapid antigen tests. (ajtmh.org)
- Rapid antigen tests have not been sufficiently evaluated in asymptomatic patients to be used as massive population screening tools. (cun.es)
- This study suggests that rapid antigen tests are less effective in asymptomatic population, when compared with RT-PCR. (cun.es)
Adaptive1
- Eggensperger S, Tampe R. The transporter associated with antigen processing: a key player in adaptive immunity. (medlineplus.gov)
Envelope1
- The viral envelope is gotten from the host cell film, and it communicates viral glycoproteins to distinguish and tie to the receptors present on the host cell layer. (alliedacademies.org)
Receptors1
- In summary, we report a ligand-based BAFF CAR-T capable of binding three different receptors, minimizing the potential for antigen escape in the treatment of B cell cancers. (lonza.com)
Respiratory1
- The role of non-viral antigens in the cotton rat model of respiratory syncytial virus vaccine-enhanced disease. (southernbiotech.com)
Detection1
- An important method in diagnostics is the identification and detection of viral capside p24 antigen levels. (nel.edu)
Isolation3
- We assessed the relationship between antigen and reverse transcription PCR (RT-PCR) test positivity and successful virus isolation. (cdc.gov)
- In this study, we expand on a previous report ( 1 ) that examined performance of antigen testing relative to RT-PCR by reporting virus isolation data for persons who had positive results by antigen test or RT-PCR. (cdc.gov)
- ECDOs (61 birds) and ROPIs (9 birds) were the only species in which PPMV-1-associated disease was confirmed by viral isolation and presence of histologic lesions. (usgs.gov)
Successfully1
- They can successfully gauge viral antigens with high explicitness and improve on the example handling and translation of the outcomes. (alliedacademies.org)
Dose3
- Each dose contains 160 U of antigen adsorbed on aluminium hydroxide (0.3 mg Al). (wikipedia.org)
- Each adult dose contains 1440 ELISA units of viral antigen adsorbed on aluminium hydroxide (0.5 mg Al). (wikipedia.org)
- Adjuvants can enhance vaccine immunogenicity, allowing a reduction in the quantity of antigen per dose and a consequent increase in the number of doses that can be manufactured in a given time-period. (nature.com)
Test1
- We found that antigen test results were more predictive of virus recovery than RT-PCR results. (cdc.gov)