• The crystal structure of the mucin monoclonal antibody SM3 complexed with a 13 amino acid MUC1 mucin peptide was determined. (wisconsin.edu)
  • Membrane bound mucins MUC1 and MUC3 appear to be associated with the development of gastric carcinoma. (bmj.com)
  • Aberrant (upregulated) expression of the MUC1 mucin has been seen in breast carcinomas and other neoplasms, such as colon and pancreatic cancers. (bmj.com)
  • 1, 2 Most studies of MUC1 expression in breast carcinomas have shown that increased membrane MUC1 mucin expression on the apical cell surface is associated with a better prognosis (reviewed in Rahn and colleagues 2 ), whereas circumferential staining in tumour cell cytoplasm is associated with a worse prognosis. (bmj.com)
  • This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
  • Glycosylated Mucin-1 (MUC1) peptide fragment containing amino acid sequence Pro126-Gly149 (24 AA in length) from the tandem repeat (TR) region of the MUC1 protein. (sussex-research.com)
  • The results demonstrated the presence of segmental duplications of CysD domains, insertions/deletions (indels) of tandem repeats, and single nucleotide variants," they note. (pacb.com)
  • From the abstract: 'Genome sequencing (GS) is one of the most comprehensive assays that interrogate single nucleotide variants, copy number variants, mitochondrial variants, repeat expansions, and structural variants in one assay. (cdc.gov)
  • The salivary MUC7 gene provides an opportunity for studying such variation, as it harbors copy number variable subexonic repeat sequences that encode for densely O-glycosylated domains (PTS-repeats) with microbe-binding properties. (nature.com)
  • The lectin showed affinity only for the glycoproteins fetuin, asialofetuin, mucin type III, and bovine submaxillary mucin type I. The complete amino acid sequences of two isoforms of ALL, named ALL-a and ALL-b, were determined by a combination of Edman degradation and overlapped peptides sequenced by tandem mass spectrometry. (bvsalud.org)
  • Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. (inrae.fr)
  • In a paper entitled " Genome Reference and Sequence Variation in the Large Repetitive Central Exon of Human MUC5AC ," published in the American Journal of Respiratory Cell and Molecular Biology, corresponding authors Wanda O'Neal and Judith Voynow along with their collaborators describe the use of Single Molecule, Real-Time (SMRT®) Sequencing to characterize a complex mucin exon. (pacb.com)
  • MUC5AC, located on the P arm of chromosome 11, encodes one of the two major secreted mucins found in our airways and contributes to gastrointestinal homeostasis through its activity in the gut. (pacb.com)
  • The characterization of MUC5AC long exon structure from the reduced representation library supported the structure of the cysD domain and the variable number tandem repeat (VNTR) regions that were observed in the PCR amplicons, validating the fidelity of the assay. (pacb.com)
  • Characterization of human mucin MUC17. (nih.gov)
  • The canonical variable number of tandem repeats polymorphism of the central tandem repeat domain of the MUC genes is not significantly detected in the MUC17 gene. (nih.gov)
  • namely, MUC3, MUC4, MUC12, MUC13, and the recently described mucin MUC17, which share a C-terminal structural domain that consists of two or three epithelial growth factor (EGF)-like regions, a transmembrane segment, and a short cytoplasmic tail. (bmj.com)
  • Primary structure was determined by tandem mass spectrometry. (bvsalud.org)
  • A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). (biomedcentral.com)
  • With increasing interest on mucins as diagnostic and therapeutic targets in cancers and other diseases, it is becoming imperative to characterize novel mucins and investigate their biological significance. (nih.gov)
  • This study investigated the correlations between aberrant expression of mucins in gastric carcinoma and patient clinicopathological features. (bmj.com)
  • Mucins are highly glycosylated proteins that serve a variety of roles in organisms from lubrication to cell-signaling to defense 7 . (nature.com)
  • ELL showed hemagglutinating activity in Ca2+ presence, which was inhibited by glycoprotein mucin and thyroglobulin. (bvsalud.org)
  • Mucin 1 lies on the tubular cell apical surface and has a role in signal transduction pathways. (medscape.com)
  • Without the accurate sequence of the exon, mucins have not been well represented in genome-wide, exome, or gene expression studies that require prior knowledge of a genetic region. (pacb.com)
  • Here, we focus on the salivary mucin-7 gene ( MUC7 ) to study the evolution and functional impact of subexonic repeats. (nature.com)
  • These repeats can be particularly well studied in this gene because the repeats are relatively short and few in number compared to other mucins. (nature.com)
  • Moreover, they demonstrated that phosphorylated NKCC2 levels rose in tandem with UMOD gene dosage. (medscape.com)
  • Epithelial mucins can be classified into two distinct families: secretory (gel forming) and membrane bound. (bmj.com)
  • In general, mucins have the unique function of protecting and lubricating epithelial surfaces, but in recent years they have also been implicated in additional diverse roles, such as growth, fetal development, epithelial renewal and differentiation, epithelial integrity, carcinogenesis, and metastasis. (bmj.com)
  • On a broader scale, our study highlights variable subexonic repeats as a primary source for modular evolutionary innovation that lead to rapid functional adaptation. (nature.com)
  • However, there is no study to our knowledge that specifically investigated the evolution of copy number variable subexonic repeats from their initial emergence as functional units to the adaptive constraints and mutational properties that contributed to their extant variation in primates. (nature.com)
  • First, MUC7 carries subexonic repeats that are 69 bp (23 amino acids) long and are copy number variable in humans 5 . (nature.com)
  • Second, the subexonic repeats of MUC7 code for densely O-glycosylated proline-, threonine-, and serine-rich (PTS) tandem repeat domains. (nature.com)
  • A recent study has shown that, despite the high intrinsic mutability of subexonic repeats, the vast majority of thousands of subexonic repeats remain strongly conserved among mammals 4 . (nature.com)
  • Several studies have reported conflicting and inconclusive results concerning the clinical relevance of mucin expression in gastric carcinoma. (bmj.com)
  • MUC7 provides an opportunity to assess the broader implications of subexonic repeat variation within the context of the functional evolution of the mucin functional group and primate salivary adaptation. (nature.com)
  • MUC7 comprises a number of interesting properties that could make it a model for studying subexonic repeats ( Fig. 1A ). (nature.com)
  • MUC5AC rich mucins contained more protein than MUC2 rich mucins. (hindawi.com)
  • Mucins are highly glycosylated protein molecules of linear and flexible amino acid polymers composed of subunits joined by disulphide bonds [ 11 - 13 ]. (hindawi.com)
  • This gene encodes a member of the gel-forming mucin protein family. (nih.gov)
  • Last two domains (Mubs5s6) of the six mucus-binding domains arranged in tandem at the C-terminus of the Lp_1643 protein of Lactobacillus plantarum was expressed in E . coli . (nature.com)
  • Finally, we explore short-tandem repeats (STRs) throughout the genome to characterise allelic diversity at 50 known disease loci, uncover hundreds of novel repeat expansion sites within protein-coding genes, and identify unique patterns of diversity and constraint among STR sequences. (researchgate.net)
  • 16. The contribution of tandem repeat number to the O-glycosylation of mucins. (nih.gov)
  • Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells. (bvsalud.org)
  • Each subunit contains highly glycosylated, proteinase-resistant regions (variable number tandem repeat, VNTR) rich in serine and/or threonine amino acids alternating with sparsely or nonglycosylated (naked), proteinase-sensitive regions [ 14 - 16 ]. (hindawi.com)
  • Uniquely, the stomach and colon contain a dual-layer of mucus that is composed of polymeric sheets of these highly glycosylated mucins ( Figure 1 ). (frontiersin.org)
  • Mucin family members are glycoproteins that have tandem repeats which are extensively O-glycosylated. (nih.gov)
  • The intestinal mucus layer is principally comprised of a subset of high molecular weight glycoproteins called mucins, which play a crucial role in physical protection as well as in regulating the concentration and passage of water, ions, and other immune mediators such as antimicrobial peptides (AMPs) and immunoglobulin-A (IgA) within the gut ( 2 , 3 , 7 ). (frontiersin.org)
  • These mucins can be broadly classified into either secreted gel-forming mucins, those that provide the structural backbone for the mucus barrier, or transmembrane mucins, those that form the glycocalyx layer covering the underlying epithelial cells. (frontiersin.org)
  • Mucins represent the major constituent responsible for mucus viscoelastic properties [ 10 ] which enables the mucus layer to achieve its protective function for the underlying mucosa. (hindawi.com)
  • We studied the sinus mucin fragmentation as this will help in the understanding of the physical and biological properties of sinus mucins in CS and could help to develop therapeutic modalities to alter these properties and facilitate mucus drainage and relieve relevant CS symptoms such as rhinorrhea, chronic cough, and globus pharyngeus. (hindawi.com)
  • Mubs5s6 showed binding with the rat intestinal mucus, pig gastric mucins and human intestinal tissues. (nature.com)
  • Native/polymeric mucins were isolated by 2 rounds of caesium chloride density gradient centrifugation [ 19 ] followed by extensive dialysis and freeze-drying. (hindawi.com)
  • The current review aims to highlight the structural and functional features as well as the production and immunological regulation of mucins and the impact these key elements have within the context of barrier function and host defense in intestinal inflammation. (frontiersin.org)
  • Characterization of human mucin MUC17. (nih.gov)
  • WS at 25-fold lower concentration than CS increased mucin expression more efficiently in mice and in human AECs in a p53 pathway-dependent manner. (nih.gov)
  • Sinus mucin fragmentation produced mucin subunits and glycopeptide units of smaller molecular sizes which are likely to have lower viscoelastic properties. (hindawi.com)
  • The potency of WS compared with CS in inducing mucin expression may explain the increased risk for chronic bronchitis in participants exposed to WS. (nih.gov)
  • Amino acid analysis was characteristic of mucins with high serine, threonine, and proline contents and reduction and proteolysis increased relative proportions of these amino acids. (hindawi.com)
  • This could be contributing to biological properties and functions of sinus mucins. (hindawi.com)
  • Next, we quantified endogenous proteoglycan 4 (PRG4), a mucin-like glycoprotein, in tear wash and saliva samples via a bead-based immune assay. (uwaterloo.ca)
  • Evidence from both clinical and animal studies have indicated that several GI conditions, including inflammatory bowel disease (IBD), colorectal cancer, and numerous enteric infections are accompanied by considerable changes in mucin quality and quantity. (frontiersin.org)
  • Mucin 1 lies on the tubular cell apical surface and has a role in signal transduction pathways. (medscape.com)
  • CONCLUSIONS: Sight restoration options are limited in patients with end-stage SJS with a keratinized ocular surface, aqueous and mucin deficiency, corneal opacification, and limbal stem cell deficiency. (ocularservices.com)
  • Extensive characterization including infrared and X-ray spectroscopy, thermogravimetric analysis, size, charge, surface area, and density captured the alteration in physicochemical properties as the material went through sequential purification. (cdc.gov)