• Researchers are continuously more engaged in high-parameter flow cytometry assays, analyzing twenty or more markers at high throughput, for deeper insights into the immune network of the host organism. (biolegend.com)
  • For example, the authors have found that AM from young children are less suppressive for T-lymphocyte proliferation using in vitro functional assays 2 , but the developmental changes of relevant immune receptors could not be identified because of the limited numbers of cells recovered. (ersjournals.com)
  • At CHI's recent "High-Content Flow Cytometry" meeting, Dr. Robinson talked about the ability to automate flow cytometry-based high-content screens and data processing to perform multiplexed high-content analysis in real-time, running multiple assays and samples in parallel, and to miniaturize the technology to conserve resources and reduce costs. (genengnews.com)
  • Automation of the entire process-from sample preparation, to running complex assays, to data analysis-makes it possible to perform functional phenotypic profiling of cell populations efficiently. (genengnews.com)
  • He described the graphical interface the group developed-a logic map designed to analyze the data from functional live-cell assays that incorporate fluorescent markers. (genengnews.com)
  • Also, while a conventional flow cytometer helps in multi-parametric analysis, it lacks spatial resolution. (e-consystems.com)
  • Flow cytometry is a technology that uses single or multiple lasers to provide a multi-parametric analysis of single cells. (sigmaaldrich.com)
  • Conventional cytometers are effective for studying high-volume cell populations but lack spatial resolution. (e-consystems.com)
  • This makes conventional cytometers good for studying high-volume cell populations. (e-consystems.com)
  • It can lead to overlapping signals and difficulty in accurately identifying distinct cell populations - resulting in inaccurate data analysis. (e-consystems.com)
  • The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imaging flow cytometry. (jove.com)
  • Numerous fields of study use flow cytometry and often analyze multiple parameters within diverse populations of cells. (sigmaaldrich.com)
  • Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)
  • This capability is particularly important when studying heterogeneous cell populations and a variety of different phosphoproteins. (genengnews.com)
  • The researchers were able to correlate cell populations with pathway activity. (genengnews.com)
  • When tandem dyes are incorporated into a flow cytometry experiment, it is possible to generate multiple readouts from the same laser. (biolegend.com)
  • Conventional flow cytometer has the advantage of high throughput: detect single cell properties at rates from hundreds to 100,000 cells per second. (e-consystems.com)
  • In clinical diagnostics and cell biology, the imaging strategies (such as the speed of the laser scanning technologies used in laser confocal microscopy), the frame rate of the image sensors, the complexity of the image processing, the computing power and optical blur brought on by cells moving at fast linear speeds are what primarily limit the imaging throughput. (e-consystems.com)
  • IFC combines the single-cell identification and high throughput of traditional flow cytometry with the cell image collection capabilities of fluorescence microscopy. (e-consystems.com)
  • MIFC gives a high throughput image by capturing multiple changes simultaneously. (e-consystems.com)
  • An ultra-high throughput imaging cytometer that uses stroboscopic lighting and inertial focusing allows sheathless manipulation of cells with no motion blur. (e-consystems.com)
  • A general overview of cell cytometry, featuring information on the different types of cytometry, namely high throughput flow cytometry and image cytometry , along with details on their advantages and limitations. (onetable.world)
  • In addition, the chapter includes analysis of high throughput flow cytometer developers, along with information on their year of establishment, company size, location of headquarters and leading players (in terms of number of products offered). (onetable.world)
  • Elaborate profiles of prominent players (shortlisted based on a proprietary criterion) engaged in the development / commercialization of cell cytometers (high throughput flow cytometers and image cytometers). (onetable.world)
  • The initial resistance that the high-content/high-throughput community had to face has been overcome, and now most in the field acknowledge that flow cytometry is enormously high throughput and very high content. (genengnews.com)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • In the past, conventional cytometers have faced insurmountable challenges. (e-consystems.com)
  • Incorporating imaging capabilities into conventional flow cytometry is highly recommended since cellular morphology analysis is crucial for many biological studies and clinical diagnoses, including cancer screening. (e-consystems.com)
  • Cells are first immobilised on a conventional glass slide by centrifugation or adherence, and after immunostaining are scanned sequentially by two lasers (argon and helium-neon, emitting at 488 and 633 nm, respectively). (ersjournals.com)
  • The Laser Detected MRI uses laser-based detection to achieve high-resolution images without the need for high magnetic fields like those used in conventional MRI machines. (osti.gov)
  • Flow cytometry is a powerful technology that is commonly used in molecular and cellular biology research applications, including immunology, cancer biology, and disease research. (sigmaaldrich.com)
  • Common molecular biology applications include analysis of recombinantly expressed fluorescent proteins that have induced expression in various cell types to elucidate gene function or for in vivo cell tracking. (sigmaaldrich.com)
  • The Nordenfelt Lab is interested in the interplay between human cells and their environment and the cell biology that controls the outcome of the interactions. (lu.se)
  • b ) Example cell lineage traces from time-lapse microscopy. (elifesciences.org)
  • c ) Scatter plot of mean C-CDK level vs cell size from time-lapse microscopy data. (elifesciences.org)
  • Cells were followed through time-lapse microscopy with measurements taken each frame. (elifesciences.org)
  • Cells were then followed and monitored for their Cut3-tdTomato nuclear/cytoplasmic (N/C) ratio (C-CDK activity) and C-CDK-YFP level using fluorescence time-lapse microscopy (see Materials and methods). (elifesciences.org)
  • To date, where AM numbers are limited or if AM morphology is required, image analysis (IA) systems such as confocal microscopy have been used 8 . (ersjournals.com)
  • However, it has the differential of providing images of all cells analyzed, since it is equipped with fluorescence microscopy objective lenses. (butantan.gov.br)
  • By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. (coremarketplace.org)
  • This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). (jove.com)
  • These monocytes isolation protocols can contribute to the development of reliable methods for the purification of human dendritic cells for research and clinical applications. (jove.com)
  • In addition, this is the first study to characterize the specific population of monocyte derived dendritic cells by single cell imaging flow cytometry. (jove.com)
  • CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
  • BD FACSDiscover ™ S8 Cell Sorter with BD CellView ™ Image Technology and BD SpectralFX ™ Technology, the first spectral flow cytometer sorter with sort-capable image analysis, expands the power of cell analysis and sorting to new dimensions by combining spectral flow cytometry with real-time spatial and morphological insights-empowering scientists to address previously impossible-to-answer questions. (bdbiosciences.com)
  • Learn more from the BD FACSDiscover ™ S8 Cell Sorter brochure . (bdbiosciences.com)
  • The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. (coremarketplace.org)
  • As a result, it becomes the perfect method for carrying out both morphological characteristic analysis and phenotypic characterization of individual cells within a massive and diverse population. (e-consystems.com)
  • In addition to maintenance of membrane integrity, apoptotic cells can be discriminated from viable counterparts based on several morphological hallmarks, including cell contraction, nuclear condensation and fragmentation, and actomyosin contraction-dependent membrane blebbing and apoptotic body formation. (nature.com)
  • This means it is imperative to understand the expression level of the target, the capabilities of the cytometer, and the properties of fluorophores used for detection, especially when multiple fluorophores will be combined in a panel. (biolegend.com)
  • Discover how to select flow cytometry dyes with tips for flow cytometry dye selection including matching fluorescence profiles of fluorophores to the configuration of flow cytometers, the factors that can affect fluorophore performance in a multicolor panel, and helpful tools for simple dye selection. (sigmaaldrich.com)
  • Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data. (coremarketplace.org)
  • In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and image-based confluence measurements for automated dilution ratio calculation. (frontiersin.org)
  • During a typical flow cytometry experiment, samples are stained using fluorophore-labeled antibodies that recognize and bind specific cell surface or intracellular markers, or a variety of fluorescent chemical probes. (biolegend.com)
  • Whatever the aim of a flow cytometry experiment, and regardless of which flow cytometry platform is used, achieving accurate results hinges on experimental design. (biolegend.com)
  • Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease. (sigmaaldrich.com)
  • Flow cytometry antibodies are used to specifically bind to different cell markers, helping to characterize the cell type and assess any changes in protein expression as part of the experiment. (sigmaaldrich.com)
  • In addition, further detailed imaging of each cell can better differentiate between cells and debris to give quantitative visualization of cells with great spatiotemporal resolution. (e-consystems.com)
  • Laser scanning cytometry (LSC) generates quantitative information on immune receptor expression from cells cytocentrifuged onto a microscope slide. (ersjournals.com)
  • 7 The goal of these processes is detection and removal of dead cells and associated debris to limit potential further damage. (nature.com)
  • Numerous types of labels can be used to visualize the antibodies for detection in the flow cytometer depending on the experimental design including nucleic acid dyes, cell viability dyes, polymer dyes, quantum dots, small organic molecules, and fluorescent proteins. (sigmaaldrich.com)
  • Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
  • Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)
  • In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
  • In children, the description of developmental changes in immune receptor expression on alveolar macrophages (AM) has been limited by the small number of cells recovered by bronchoalveolar lavage (BAL). (ersjournals.com)
  • It is concluded that laser scanning cytometry is suited to the study of immune receptor expression from small numbers of paediatric alveolar macrophages, when CD68 is used for cell identification. (ersjournals.com)
  • Immune cell responses induced by DAMPs may include cytokine and chemokine release to activate inflammatory cells such as neutrophils and macrophages. (nature.com)
  • Zhang G, Li Q, Tao W, Qin P, Chen J, Yang H, Chen J, Liu H, Dai Q, Zhen X. Sigma-1 receptor-regulated efferocytosis by infiltrating circulating macrophages/microglial cells protects against neuronal impairments and promotes functional recovery in cerebral ischemic stroke. (thno.org)
  • Accumulating evidence indicates that immune cells, especially brain-resident microglia and infiltrated circulating macrophages, are intimately involved in the pathological development of ischemic stroke [ 2 , 3 ]. (thno.org)
  • A large amount of damage-associated molecular patterns (DAMPs) released by all types of dead cells trigger inflammatory responses by activating microglial cells or macrophages [ 5 ]. (thno.org)
  • Find answers to frequently asked questions (FAQs) about ColorWheel ® flow cytometry antibodies and dyes, straight from the experts. (sigmaaldrich.com)
  • Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows. (sigmaaldrich.com)
  • View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining. (sigmaaldrich.com)
  • With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel ® flow cytometry antibodies and dyes. (sigmaaldrich.com)
  • A new proprietary flow cytometry technology allows end users to flexibly mix and match independently selected antibodies and dyes to assemble them in any combination, without the use of conjugation kits or secondary antibodies. (sigmaaldrich.com)
  • These dyes or biomarkers can be used in conjunction with visible and near-infrared light to highlight specific cell and tissue types, such as metastatic cancer cells circulating in the bloodstream. (nationalacademies.org)
  • Flow cytometry, which first began to develop in the 1950s, is a technology for characterizing the phenotype and functionality of individual cells within a heterogeneous population. (biolegend.com)
  • A fluorescent image of a cell moving faster than the motion-blur velocity can be recreated using this method. (e-consystems.com)
  • Alternatively, the cells themselves may be engineered to tie the expression of a protein of interest to a reporter such as Green Fluorescent Protein ( GFP ). (biolegend.com)
  • Critically, as flow cytometry has advanced, the number of fluorescent readouts that can be measured simultaneously has increased, along with the sample processing speed. (biolegend.com)
  • Using FC, fluorescent antibodies have attached to immune receptors on cells which are then identified as AM by their characteristic autofluorescence, light scatter properties ( e.g. forward scatter) or a combination of both 6 . (ersjournals.com)
  • The software recognises clusters of fluorescent pixels (pixelated clusters), these clusters being the primary "triggering" parameter for each cell. (ersjournals.com)
  • To date, the most common method of capturing cells is for the software to identify nuclei stained with a fluorescent dye 11 - 13 . (ersjournals.com)
  • Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)
  • A large number of the Prestige Antibodies have been used in subcellular localization studies by immunofluorescence (IF) staining of three cell lines: A-431, U-2 OS, and U-251MG. (sigmaaldrich.com)
  • Performing flow cytometry analysis involves using various flow cytometry antibodies and labels. (sigmaaldrich.com)
  • The main advantage of these protocols is that they facilitate the isolation of human monocytes and their differentiation into viable and functional monocyte derived cells. (jove.com)
  • Stem Cell Research: Includes the process of cell differentiation. (labroots.com)
  • The equipment allows the quantification of fluorescence intensity in suspended cells, a characteristic common to all flow cytometers. (butantan.gov.br)
  • Get insights into flow cytometers and how the right cameras can greatly improve their performance. (e-consystems.com)
  • In this blog, you'll get in-depth insights into how image-based flow cytometers work and why they're highly popular in modern applications. (e-consystems.com)
  • Expanding the palette of colors provides in-depth single-cell insights that can be isolated with sorting for further downstream studies. (bdbiosciences.com)
  • An analysis of various recent developments / trends related to global cell cytometry domain, offering insights on the funding activity in this domain, based on several relevant parameters, such as year of funding, type of funding, amount invested (USD Million), most active players (in terms of number of instances and amount raised), and most active investors (in terms of number of instances). (onetable.world)
  • Cell surface sensors on immune cells that ordinarily recognize pathogen-associated molecular patterns produced by infectious agents may also interact with DAMPs, leading to the activation of signaling pathways resulting in a nonpathogen-induced 'sterile' inflammatory response. (nature.com)
  • Neutrophils are the first immune cells that infiltrate the damaged tissue as early as 24 h after injury. (springer.com)
  • This could be immune cells trying to find their way to invading bacteria and viruses or cancer cells migrating out of a tumor. (lu.se)
  • These devices find it tough to distinguish particles or cells close in size as the spatial resolution is determined by the size of the laser beam and the optics used to detect scattered light. (e-consystems.com)
  • All existing data (satellite images, variability of winds,submarine measurements) say this a highly variable system", said Bindschadler. (nasa.gov)
  • The increasing use of automation and miniaturization is lowering the cost of flow cytometry-based high-content analysis in primary and secondary drug screening, making it possible to run larger screening campaigns. (genengnews.com)
  • 10 9 apoptotic events occurring per day in human adult tissues, it is surprisingly difficult to histologically detect apoptotic cells due to the rapid recognition and clearance of apoptotic cells. (nature.com)
  • Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. (thermofisher.com)
  • Cell size in pixels 2 is given in orange, and C-CDK-YFP fluorescence intensity is given in purple. (elifesciences.org)
  • The Cellomics Arrayscan VTI instrument is an imaging tool that will help our scientists make quicker and more precise analyses, capturing, for instance, changes in cell size, shape and intensity. (lu.se)
  • This method can also be applied as an alternative for the isolation of other rare cells at an adequate yield for downstream research applications. (jove.com)
  • The technology enables you to answer complex biological questions, such as how cells grow, function and interact, or to study exact locations of viruses or proteins within a cell, all at a highly accelerated pace. (bdbiosciences.com)
  • Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
  • Images convey valuable information about cell shape, size, morphology, and distribution of labeled biomolecules within cells. (e-consystems.com)
  • it cannot display the staining morphology without additional cell sorting, AM cannot be restained and must be processed either when viable or when freshly fixed. (ersjournals.com)
  • Separate the cells by centrifugation, followed by the transfer of the white blood cell interface layer into a new 50 milliliter conical tube. (jove.com)
  • Oocysts from 21 human stool specimens were separated from other stool components using discontinuous density gradient centrifugation and purified further by flow cytometry. (cdc.gov)
  • Using flow cytometry, researchers can measure cell size and granularity as well as investigate factors including viability, cell cycle, and protein expression. (biolegend.com)
  • Spectrum Image Cytometer is an entire customizable cell counting and cytometry system that provides flow-like data with predesigned templates to provide imaging, cell count, concentration, and viability data. (nexcelom.com)
  • A detailed assessment of the market landscape of image cytometers based on several relevant parameters, such as type of image cytometer, processing time, type of plate format, output format, sample volume, and application(s). (onetable.world)
  • An insightful analysis of the partnerships that have been inked within the global cell cytometry industry since 2017, based on several relevant parameters, such as year of partnership, type of partnership (acquisitions, asset acquisitions, commercialization agreements, commercialization and distribution agreements, distribution agreements, product development agreements storage and distribution agreements and others), most active players (in terms of number of deals inked) and regional distribution of partnership activity. (onetable.world)
  • Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)
  • Although spectral cytometry can seem intimidating or complex, our advanced spectral cytometry system offers you a guided user-interface including individually adjustable spectral detectors, auto-alerts if a detector is saturated and the flexibility to easily assign an autofluorescence-matched negative for each single color control. (bdbiosciences.com)
  • Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. (coremarketplace.org)
  • This method allows for multiplexed analysis of cell types and signaling pathways, and has applications in drug target identification and throughout the drug development workflow, including in the analysis of patient samples during clinical testing. (genengnews.com)
  • however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. (coremarketplace.org)
  • Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • To achieve a proper separation of the peripheral blood mononuclear cells, load the blood onto the density gradient solution quickly, but carefully and without mixing the layers. (jove.com)
  • In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
  • Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
  • ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. (coremarketplace.org)
  • Hence, the ability of traditional flow cytometers to discriminate between particles of similar size becomes challenging. (e-consystems.com)
  • Check out this flow cytometry troubleshooting guide with possible causes and solutions for most common problems encountered during flow cytometry experiments, including a loss or lack of signal, high background, decreasing acquisition rates, and more. (sigmaaldrich.com)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. (coremarketplace.org)
  • At both high (x ray) and low (radio frequency [RF]) energies the body is mostly transparent, thus allowing the non-invasive imaging of the internal structure of organs and bones. (nationalacademies.org)
  • CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
  • 60% of circulating tumor cells. (nature.com)
  • In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
  • Together, these studies identify T cell-mediated tumor regression as the mechanism through which a plant-derived essential oil diminishes established tumor burden. (biorxiv.org)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Stem cells hold tremendous potential in healthcare and research science, because they can become any type of cell. (labroots.com)
  • However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
  • However, data collection by IA is time consuming since all cells need to be examined by the operator and this may lead to significant subjective bias. (ersjournals.com)
  • For paediatric AM, the ideal technique should be automated, generate FC-type data, use very small numbers of cells and be able to process stored fixed cells. (ersjournals.com)
  • To obtain immune receptor data from the cell surface and cytoplasm after nuclear capture, a "contour" is generated at a fixed distance from the nuclear perimeter. (ersjournals.com)
  • Dr. Robinson also discussed how data-extraction issues have historically been a stumbling block in the use of flow cytometry in drug discovery. (genengnews.com)
  • BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. (coremarketplace.org)
  • Thus, development of strategies targeting particular subsets of inflammatory cells and providing well-timed resolution of inflammation may be crucial for proper healing and recovery. (springer.com)
  • The vectors are also used for in vivo modifications of cells, e.g. for reprogramming. (lu.se)
  • Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
  • Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
  • Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD14 PerCP-eFluor® 710 (purple histogram). (thermofisher.com)
  • These restrictions make it difficult to do analysis and large-scale single-cell imaging. (e-consystems.com)
  • We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
  • Need tips on how to improve your flow cytometry analysis? (sigmaaldrich.com)
  • In addition, Dr. Robinson presented an example of multicolor flow analysis to demonstrate the ability to perform cytomics studies for pathway analysis. (genengnews.com)
  • Apoptosis allows for efficient and immunologically silent removal of damaged or superfluous cells in multicellular organisms. (nature.com)
  • At an early stage, blocking nutrition and oxygen supply in response to brain ischemia activates various cell death processes, including apoptosis, autophagy and necrosis [ 4 ]. (thno.org)
  • Points represent cells binned by size, with points plotted at bin centre. (elifesciences.org)
  • To isolate monocytes by the adherence method, culture five times 10 to the seven cells of the resulting PBMC pellet per 10 milliliters of complete medium in a new T75 flask for two hours at 37 degrees celsius and five percent carbon dioxide in a humidified incubator. (jove.com)
  • for example, a particular drug might inhibit kinase activity in T cells but not in monocytes. (genengnews.com)
  • However, the advent of image-based flow cytometers has created the opportunity to build cutting-edge, highly productive platforms. (e-consystems.com)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • The amount of this intrinsic absorption can be used to determine the physiological health of an organ-for example, whether the tissue is getting sufficient oxygenated blood flow. (nationalacademies.org)
  • The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)
  • Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
  • At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • The main goal of research on embryonic stem cells is to learn how blank stem cells turn into differentiated stem cells. (labroots.com)