Cells microscopy immunoelectron. Medical search. Frequent questions

Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
Caneva L, Soligo D, Cattoretti G, De Harven E, Deliliers GL (1995) Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. (atsbio.com)
These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
Immuno-electron microscopy, also referred to as EM immunolabelling and immuno-EM, is a technique that tags antibody molecules with electron-dense substances, usually, and most effectively, being small gold particles, which are seen during the analysis as easy to spot dark dots. (news-medical.net)
In the latest reports, electron microscopy, immuno-electron microscopy, and culturing from brain tissues were not performed. (alzforum.org)
Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. (cellimagelibrary.org)
TEM, transmission electron microscopy. (rupress.org)
Both immunofluorescence and transmission electron microscopy analyses of cells overexpressing Clk/STY indicate that IGC components are completely redistributed to a diffuse nuclear localization, leaving no residual structure. (rupress.org)
Transmission electron microscopy and energy-dispersive X-ray microanalysis confirmed the presence of Al specifically in intracytoplasmic electrondense nanodeposits adjacent to mitochondria of colon cancer cells. (bvsalud.org)
She is also head of the Cell Microscopy Core of the UMC Utrecht, which is widely recognized as one of the best expertise centers in the world for immuno-electron microscopy. (umcutrecht.nl)
2009-2014: WP4: Coordinator for Correlative Light Electron Microscopy. (umcutrecht.nl)
The rough cells had long, thin fibrillar structures outside the cell envelope when they were stained negatively for electron microscopy. (microbiologyresearch.org)
The techniques that we use range from single molecule mechanics, (immuno) electron microscopy, exon microarray analysis, in vitro motility assays, low angle X-ray diffraction, cell physiology (including calcium imaging), muscle mechanics, and isolated heart physiology. (bio5.org)
Correlative Light- and Electron Microscopy with chemical tags , Mario Perkovic a , Michael Kunz a , Ulrike Endesfelder b , Stefanie Bunse c , Christoph Wigge a , Thou Yu a , Victor-Valentin Hodirnau a , Margot P.Scheffer a . (leica-microsystems.com)
Using immuno-gold electron microscopy of plasma membrane sheets coupled with spatial point analysis of validated microdomain markers, morphologically featureless microdomains were visualized in HeLa cells at high resolution. (tamu.edu)
By electron microscopy, most DβH terminals did not make synapses, but when they did, they formed both asymmetric and symmetric synapses. (frontiersin.org)
By electron microscopy, βAR 248 was present in neuronal cell bodies, dendritic shafts and spines, and some axon terminals and astrocytes. (frontiersin.org)
2011). Using dual-immuno-electron microscopy we further showed that early tau alterations initiate in Aβ accumulating synaptic terminals in the brain (Takahashi et al. (lu.se)

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. (antikoerper-online.de)
In the present study, we investigated the localization of PRKRA in the mammalian testis by immunofluorescence and immunoelectron microscopic techniques. (hindawi.com)
Western blot analysis and immunofluorescence experiments showed that PPI reduced the expression of GRP78, E-cadherin, N-cadherin, Vimentin, and ABCG2 in SR-HCC cells. (bvsalud.org)
Direct immunofluorescence microscopy performed on epithelial biopsy specimen obtained from a patient with pemphigus vulgaris detects immunoglobulin G deposits at the epithelial cell surfaces. (medscape.com)

In confirmation of recent results obtained by light microscopy, somatostatin was found in all the typical D-cells containing large, weakly electron-dense secretory granules. (diabetesjournals.org)

In order to correlate the different cell types of the human endocrine pancreas to a specific secretion product, an immunoelectron microscopic localization of the hormones whose production had been attributed to pancreatic islets was conducted. (diabetesjournals.org)
Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
The objective of this study was to identify the PDE10A isoforms expressed in the testis and germ cells, and to determine the presence and localization of PDE10A in mature spermatozoa. (plos.org)

In this work, parallel infections with ZIKV of African and Asian lineages, DENV, and YFV-17D were compared for kinetics and growth efficiency, activation of mTOR pathways, and cytokine secretion profile using cytotrophoblast-derived HTR8 cells and monocytic U937 cells differentiated to M2 macrophages. (bvsalud.org)
By comparing parallel infections of Zika virus strains belonging to the African and Asian lineages, dengue virus, and the yellow fever vaccine virus strain YFV-17D in placenta-derived cytotrophoblast cells and differentiated macrophages, evidence was found that Zika virus infections, especially by the African strains, were more efficient in cytotrophoblast cells than dengue virus or yellow fever vaccine virus strain infections. (bvsalud.org)

Using double-labelling immunoelectron microscopic techniques, co-clustering between GABA B1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. (springer.com)

It is highly likely that the peculiar morphology of the hair cell/calyx synapse has consequences for synaptic transmission. (jneurosci.org)
Immunoelectron microscopy revealed excitatory synaptic connections between regenerating host axons and graft-derived neurons at C1 as well as between graft axons and DCN neurons in the brainstem. (jneurosci.org)
To understand better the cellular mechanisms of NE's contributions to fear learning, we examined the anatomical organization of NE terminals and βARs in the LA. In this study, we employed immunoelectron microscopy to determine whether terminals immunoreactive for dopamine beta-hydroxylase (DβH), the synthetic enzyme for NE, form synaptic junctions in the LA and if so, examine these synapses and identify the post-synaptic targets on NE terminals. (frontiersin.org)
Here we studied the synaptic inputs from the host brain to grafted cortical neurons derived from human induced pluripotent stem cells after transplantation into stroke-injured rat cerebral cortex. (lu.se)
Using the rabies virus-based trans-synaptic tracing method and immunoelectron microscopy, we demonstrate that the grafted neurons receive direct synaptic inputs from neurons in different host brain areas located in a pattern similar to that of neurons projecting to the corresponding endogenous cortical neurons in the intact brain. (lu.se)

A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
In the RRBP1 -knockdown Calu-6 cells, a human renin-producing cell line, transmission electron and confocal microscopy revealed that renin was primarily retained in the endoplasmic reticulum and was unable to efficiently target the Golgi apparatus for secretion. (biomedcentral.com)
Macula densa senses electrolytes concentration in the distal convoluted tubules and modulate renin secretion from JG cells in the kidney. (biomedcentral.com)

In situ hybridization and immunohistochemical studies have shown that Purkinje cells (PCs), the output neurons of the cerebellar cortex, are the neuron type with the highest levels of GABA B receptors (Bowery et al. (springer.com)
An immunohistochemical study revealed renin accumulation in the juxtaglomerular cells of Rrbp1 -KO mice. (biomedcentral.com)
Immunohistochemical and immunoelectron study of major histocompatibility complex class-II antigen in canine cutaneous histiocytoma: its relation to tumor regression. (illumina.com)

Confocal microscopy revealed that both excitatory and inhibitory neurons express βAR248. (frontiersin.org)

Virus was detected in serum by counter-current immunoelectrophoresis (CIE) and immunoelectron microscopy (IEM). (bmj.com)

A rabbit-anti-mouse Alexa-488 bridging antibody was used to locate infected cells by on-grid correlative light and EM. (biomedcentral.com)

With immunoelectron microscopy in pemphigus erythematosus, IgG and C3 deposits are localized to the epidermal cell membranes and the upper dermis. (medscape.com)
Whereas the pemphigus subset of diseases is mediated by autoantibodies that target the extracellular skin components that link one epidermal cell to another, the pemphigoid subset is mediated by autoantibodies that target the extracellular skin components that link the skin basement membrane components either to the lowermost layer of epidermal cells or to the dermal components. (medscape.com)

Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
MTT assays demonstrated that memantine was able to upregulate neuronal cell survival, and acridine orange staining and flow cytometry demonstrated that memantine (5 µM) was able to inhibit neuronal autophagy and apoptosis. (spandidos-publications.com)

In the vestibular periphery a unique postsynaptic terminal, the calyx, completely covers the basolateral walls of type I hair cells and receives input from multiple ribbon synapses. (jneurosci.org)
Type I hair cells contain ∼7-22 individual ribbon synapses per hair cell ( Lysakowski and Goldberg, 2008 ). (jneurosci.org)
2005). A major effort of our group has been to determine the cell biological mechanism(s) by which Aβ peptides initiate dysfunction of synapses in AD. (lu.se)

Transient binding of the imager strands achieves the ON/OFF switching of fluorescence signals necessary for SML microscopy. (nature.com)

Transplantation of neural progenitor cells (NPC) is a promising therapeutic strategy for replacing neurons lost after spinal cord injury, but significant challenges remain regarding neuronal integration and functional connectivity. (jneurosci.org)
By light microscopy, βARs were present in both neurons and astrocytes. (frontiersin.org)
Clusters of neurons in the somatic peripheral nervous system which contain the cell bodies of sensory nerve axons. (lookformedical.com)
Transplanted neurons derived from stem cells have been proposed to improve function in animal models of human disease by various mechanisms such as neuronal replacement. (lu.se)
The present study demonstrates, for the first time, that the host brain regulates the activity of grafted neurons, providing strong evidence that transplanted human induced pluripotent stem cell-derived cortical neurons can become incorporated into injured cortical circuitry. (lu.se)

A third, more recent research focus has been on endosymbiotic bacteria that manipulate their eukaryotic hosts by secreting enzymes, including ubiquitin-specific proteases, into the host cell cytoplasm. (yale.edu)
This group also found that while both receptor subtypes were seen in the membranes and cytoplasm of cell bodies, the β 2 receptor subtype, but not the β 1 , was localized to the nucleus. (frontiersin.org)

To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. (rupress.org)
However, both the accumulation of splicing factors at sites of pre-mRNA synthesis as well as pre-mRNA splicing are dramatically reduced, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei. (rupress.org)
Further experiments revealed that memantine was able to upregulate the expression of signaling molecules phosphorylated (p)-phosphoinositide 3‑kinase, p‑Akt and p‑mammalian target of rapamycin (mTOR), and also inhibited the phosphorylation of the B‑cell lymphoma 2/Beclin‑1 complex via mitogen‑activated protein kinase 8. (spandidos-publications.com)

Reverse transcription‑quantitative polymerase chain reaction and western blot analyses demonstrated that the AD cell model was successfully established. (spandidos-publications.com)
In addition, quantitative immunoelectron microscopy of AQP2 labeling in the IMCD principal cells showed that there was a reduction in AQP2 in the apical plasma membrane, as well as in the basolateral plasma membrane and intracellular vesicles. (artscolumbia.org)

In most human cells, mitochondria contain 10 3 -10 4 copies of a circular genome of 16,569 base-pairs that encodes two ribosomal RNAs, 22 tRNAs, and 13 polypeptides that form parts of the respiratory-chain located in the inner mitochondrial membrane [ 9 , 10 ]. (biomedcentral.com)
Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
The reduction in AQP2 (and AQP3) expression may be caused by a lithium-induced impairment in the production of cAMP in collecting duct principal cells (38, 39), indicating that inhibition of cAMP production may in part be responsible for the reduction in AQP2 expression as well as the inhibition of targeting to the plasma membrane in response to lithium treatment. (artscolumbia.org)
Inside the cell, activated integrins anchor the actin cytoskeleton to the plasma membrane (Burridge et al. (ppbhg.org)

Mitochondria are the centre of energy production in the cell. (biomedcentral.com)

More generally, we study the reversible enzymatic coupling of proteins to other proteins within cells. (yale.edu)
One such protein that we study, SUMO, is attached to many proteins and is crucial for cell-cycle progression. (yale.edu)
First, we wish to understand, at a mechanistic and molecular level, how specific proteins are rapidly degraded within eukaryotic cells while most proteins are spared. (yale.edu)
The Ubl called SUMO is attached to many proteins in vivo and is crucial for cell-cycle progression. (yale.edu)
Western blot allows researchers to determine and quantify the proteins existent within a cell, identifying specific proteins out of the mixture of proteins that are present in cell samples. (news-medical.net)
Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid. (cellimagelibrary.org)
In addition, we determined that several nuage proteins localize to spermatogenic cell-specific structures, including mitochondria-associated granules (MAG), granulated bodies (GB), reticulated bodies (RB), and ribosome aggregates (RA) [ 22 - 26 ]. (hindawi.com)
The peroxisomal ATP-binding cassette (ABC) proteins, adrenoleukodystrophy protein (ALDP, ABCD1) and ALD-related protein (ALDRP, ABCD2), were expressed in Spodoptera frugiperda 21 (Sf21) insect cells using a baculovirus-mediated expression system. (nih.gov)
We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
Control of actin filament lengths and dynamics is important for cell motility and architecture and is regulated in part by capping proteins that block elongation and depolymerization at both the fast-growing (barbed) and slow-growing (pointed) ends of the filaments. (bio5.org)
At the core of the focal adhesion is a cluster of activated integrin heterodimers, which are transmembrane signaling proteins that attach cells to their ligands, i.e., extracellular matrix (ECM)1 (Hynes, 1992). (ppbhg.org)

Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)

d Segmentation of the cell surface ( grey ) and IPMC membranes ( black ). (biomedcentral.com)

We investigated the presence of aluminium (Al) in human colon cancer samples and its potential association with biological processes involved in cancer progression, such as epithelial to mesenchymal transition (EMT) and cell death. (bvsalud.org)

In this study, we sought to determine the effect of DHA on cell surface microdomain organization in situ. (tamu.edu)

Our lab has as its general focus one of the fundamental regulatory systems of eukaryotic cells - the ubiquitin system. (yale.edu)
RISC assembles into processing bodies (P-bodies) which are specific cytoplasmic foci in somatic eukaryotic cells [ 7 - 9 ]. (hindawi.com)

Schwann cells wrap myelin around peripheral nerve axons for fast neural transmission 1 . (biorxiv.org)

In Pmp22 tg CMT1A mice, we uncovered that the differentiation defect of Schwann cells is independent from PI3K/Akt/mTOR activity, rendering the pathway insufficient as a therapy target on its own. (biorxiv.org)

We study whether PRKRA localizes to nuage of spermatogenic cells of rat and mouse. (hindawi.com)
We used compound heterozygote mice to study the long-term fate of oligodendrocytes expressing either the jimpy or rumpshaker allele against a background of cells expressing a Plp -null allele. (rupress.org)
In this study, we sought to investigate the mechanism underlying the inhibitory effect of PPI on the invasion and metastasis of SR-HCC cells. (bvsalud.org)
The present study aimed to establish an AD cell model overexpressing the 695‑amino‑acid Swedish mutant of Aβ precursor protein (APP695swe) in order to observe the effects of memantine on the cell viability, autophagy and apoptosis of SH‑SY5Y cells in the AD model, and to investigate the associated underlying mechanisms. (spandidos-publications.com)
In conclusion, the results of the present study demonstrated that in the AD cell model, autophagy was able to promote apoptosis. (spandidos-publications.com)
Ultrastructural study of leprosy granuloma and blood vessels using immunoelectron-microscopy and molecular methods. (icmr.nic.in)

Whole-cell patch-clamp recordings from calyx endings were performed in an in vitro whole-tissue preparation of the rat vestibular crista, the sensory organ of the semicircular canals that sense head rotation. (jneurosci.org)
Interference with and overproduction of GRP78 in vitro impacted the proliferation, migration, invasion, and metastasis of HCC cells. (bvsalud.org)

The final immunostaining method is the Western blot method, a widely used technique that has firmly ingrained itself in the fields of cell and molecular biology. (news-medical.net)
Since 2001 Judith Klumperman is professor of Cell Biology and since 2008 chair of the Department of Cell Biology at the UMC Utrecht. (umcutrecht.nl)
Associate edito r: Molecular Biology of the Cell. (umcutrecht.nl)
Current Opinion in Cell Biology. (umcutrecht.nl)

As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)

Human induced pluripotent stem cell-derived neural stem/progenitor cell (hiPSC-NS/PC)-based cell transplantation has emerged as a groundbreaking method for replacing damaged neural cells and stimulating functional recovery, but its efficacy is strongly influenced by the state of the injured spinal microenvironment. (biomedcentral.com)
Following cell transplantation into a favorable environment, the transplanted NS/PCs survived well, facilitating remyelination and neuronal regeneration in host tissues. (biomedcentral.com)

To date it remains unclear how exactly the hair cell receptor potential is converted into an afferent firing pattern at this unusual synapse. (jneurosci.org)
We report the use of multiplexed super-resolution imaging (Exchange-PAINT) followed by mean-shift clustering and random forest analysis to measure the precise distributions of five receptor tyrosine kinases (RTKs) from the ErbB, IGF-1R and Met families in breast cancer cells. (nature.com)
Here we exploit the multiplexing capabilities of Exchange-PAINT, a multiplexed variant of DNA-PAINT 14 , to image simultaneously five RTKs (EGFR, ErbB2, ErbB3, IGF-1R and Met) at endogenous levels of expression in BT20 cancer cells and to examine how receptor distribution changes following ligand stimulation. (nature.com)
The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)
Se evaluó la expresión de los receptores hormonales (receptor de estrógeno (RE) y de progesterona (RP)), HER2, Ki67 y GRB7, mediante inmunohistoquímica (IHQ), y a partir de estos, se clasificaron los tumores en subtipos intrínsecos. (bvsalud.org)

Much of our work is conducted in baker's yeast, a model organism ideal for genetic and biochemical analysis, but we also use human tissue culture cells and Drosophila in certain studies. (yale.edu)
The method involves selectively identifying antigens in a sample of cells within a tissue section through the principle that certain antibodies will bind to specific antigens present in the tissue. (news-medical.net)
Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. (cellimagelibrary.org)
The acute-phase HGF-treated group exhibited vascularization, diverse anti-inflammatory effects, and activation of endogenous neural stem cells after SCI, which collectively contributed to tissue preservation. (biomedcentral.com)
Tissue engineering, combining different cell types, scaffolds, growth factors, and physical stimulation has become an alternative for repairing and regenerating articular cartilage. (bvsalud.org)
Dental Follicle Mesenchymal Stem Cells (DFMSCs) are attractive candidates for cartilage tissue engineering because of their ability to differentiate into chondrocytes, on the other hand, the polymers blend like Polycaprolactone (PCL) and Poly Lactic-co-Glycolic Acid (PLGA) have shown promise given their mechanical properties and biocompatibility. (bvsalud.org)
Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). (ucsd.edu)
1995). Focal adhesions were first identified in tissue-culture cells, as sites of contact between a cell and its underlying substrate (Abercrombie et al. (ppbhg.org)

Y.Y. Fan, L.H. Ly, R. Barhoumi, D.N. McMurray, R.S. Chapkin, Dietary docosahexaenoic acid suppresses T cell protein kinase Ctheta lipid raft recruitment and IL-2 recruitment, J. Immunol. (tamu.edu)

These results suggest that each of the four cell types that are easily identifiable by ultrastructural observations is responsible for the production of a specific secretory product. (diabetesjournals.org)

With antibodies that reacted with the gastrin cells in the human gastric mucosa, it was not possible to detect gastrin in any of the islet cell types. (diabetesjournals.org)
The human pancreatic polypeptide (HPP), a newly postulated hormone, was clearly associated with a fourth cell type, which is characterized by the presence of small secretory granules (100-150 nm. (diabetesjournals.org)
We analyzed the organization and function of mitochondrial DNA in a stable human cell line (ECV304, which is also known as T-24) containing mitochondria tagged with the yellow fluorescent protein. (biomedcentral.com)
We now examine the organization of mitochondrial genomes in a human cell line that contains mitochondria tagged with the yellow fluorescent protein (YFP). (biomedcentral.com)
We further investigated the role of the RRBP1 gene using a transgenic mouse model and a human cell model. (biomedcentral.com)

a A representative cell from Donor 429 located by on-grid immunolabelling. (biomedcentral.com)
e Low power overview of the cell profile as seen emerging from under the grid bar. (biomedcentral.com)

J Cell Biol (2002) 158 (4): 719-729. (rupress.org)
J Cell Biol (2002) 156 (3): 425-436. (rupress.org)

Journal of Muscle Research and Cell Motility. (wikipedia.org)

The slides were counterstained with hematoxylin and subsequently analyzed by light microscopy. (cdc.gov)

We are trying to understand the functional consequences of SUMO-protein modification, particularly in the cell cycle and chromatin-mediate gene transcription, and to determine the basis of specificity for the SUMO-cleaving proteases. (yale.edu)
Spinal cord injury (SCI) is characterized by cell death and loss of connectivity with permanent functional deficits. (jneurosci.org)

HTR8 cells treated with mTOR inhibitors showed a 20-fold reduction in ZIKV yield, versus 5- and 3.5-fold reductions for DENV and YFV-17D, respectively. (bvsalud.org)
Robust activation of the mTOR signaling pathways and inhibition of the IFN and chemoattractant response appear to be related to the better growth capacity of the Zika viruses in the cytotrophoblast-derived cells. (bvsalud.org)

It increases natural killer cell activity and decreases markers of inflammation. (scitcentral.com)

Here, we analysed the distribution of GABA B receptors using highly sensitive SDS-digested freeze-fracture replica labelling in mouse cerebellar Purkinje cells. (springer.com)
Stimulation of postsynaptic GABA B receptors generally triggers inhibition of adenylate cyclase and activation of G protein-gated inwardly rectifying K + (GIRK/Kir3) channels, leading to cell hyperpolarisation (Kaupmann et al. (springer.com)
ErbB receptors comprise a family of four RTKs (EGFR/ErbB1, Her2/ErbB2, Her3/ErbB3 and Her4/ErbB4) with important roles in normal cell physiology and in cancer 1 . (nature.com)

In living cells, foci have an apparent diffusion constant of 1.1 × 10 -3 μm 2 /s, and mitochondria always split next to a focus to distribute all DNA to one daughter. (biomedcentral.com)
On the other hand, there are specific cytoplasmic foci similar to the P-bodies in spermatogenic cells, which are called nuage [ 15 - 18 ]. (hindawi.com)

Moreover, Al-positive samples exhibited a significant decrease in the number of apoptotic cells, as well as the expression of the anti-apoptotic molecule BCL-2. (bvsalud.org)
Further examination revealed that PPI hindered the expression of GRP78 protein, resulting in a suppressive effect on SR-HCC cell migration and invasion. (bvsalud.org)
As discussed below, a number of rat models with NDI have been evaluated, and common for all is a reduced expression of AQP2 in the principal cells of the collecting ducts. (artscolumbia.org)

These findings provide the first evidence for the ability of NPC to form a neuronal relay by extending active axons across the injured spinal cord to the intended target establishing a critical step for neural repair with stem cells. (jneurosci.org)

Polyphyllin I (PPI), a promising pharmaceutical candidate, has shown potential therapeutic advantages in the treatment of sorafenib-resistant hepatocellular carcinoma (SR-HCC cells). (bvsalud.org)

When the physical and chemical characteristics of cells or particles are sought to be determined, flow cytometry is often the most suitable method. (news-medical.net)

Such turnover occurs primarily through the ubiquitin-proteasome system and is central to a variety of cell regulatory mechanisms, many of medical relevance including many cancers. (yale.edu)

The scaffold showed to be a non-toxic effect when we evaluated it with Alamar blue, and the samples were analyzed using SEM and phalloidin staining to evaluate cell adhesion to the scaffold. (bvsalud.org)

In juxtaglomerular cells, deficiency of RRBP1 reduced renin intracellular trafficking from ER to Golgi apparatus. (biomedcentral.com)

Anatomy16

Organisms2

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes4

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science3

Electron microscopy18

Immunofluorescence4

Obtained by light microscopy1

Localization3

Macrophages2

Microscopic techniques1

Synaptic5

Secretion4

Immunohistochemical3

Confocal1

Immunoelectrophoresis1

Antibody1

Pemphigus2

Assays2

Synapses3

Fluorescence1

Neurons5

Cytoplasm2

Mammalian3

Quantitative2

Membrane4

Centre1

Proteins12

Testis1

Membranes1

Progression1

Situ1

Eukaryotic cells2

Transmission1

Differentiation1

Study6

Vitro2

Biology4

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Receptor8

Tissue8

Protein kinase1

Ultrastructural1

Human5

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Motility1

Light1

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MTOR2

Inflammation1

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Primarily1

Adhesion1

Intracellular1