Cell migration matrix metalloproteinase 9. Medical search. Frequent questions

Breast cancer stem cells (BCSCs) play a critical role in chemoresistance, metastasis, and poor prognosis of breast cancer. (hindawi.com)
is known to exert anticancer effects, such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. (hindawi.com)
Matrix metalloproteinases (MMPs) play an important role in cancer progression by degrading extracellular matrix components, promoting tumor cell migration, and thus enhancing tumor metastasis. (suny.edu)
The goal of this work is to elucidate novel molecular mechanisms that can be therapeutically targeted to regulate cell migration in a tissue-specific fashion - to inhibit it in certain contexts (e.g., inflammatory diseases and cancer metastasis) and promote it in others (e.g., stem cell transplantation and cell-based therapies). (bu.edu)
As matrix metalloproteinase-9 (MMP-9) plays a critical role in tumor metastasis, we analyzed its expression with celastrol treatment. (spandidos-publications.com)
Chambers AF and Matrisian LM: Changing views of the role of matrix metalloproteinases in metastasis. (spandidos-publications.com)
Chung TW, Moon SK, Chang YC, et al: Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepato-carcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. (spandidos-publications.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
In this study, we sought to investigate the mechanism underlying the inhibitory effect of PPI on the invasion and metastasis of SR-HCC cells. (bvsalud.org)
Interference with and overproduction of GRP78 in vitro impacted the proliferation, migration, invasion, and metastasis of HCC cells. (bvsalud.org)
MMP-9 (matrix metalloproteinase 9) cleaves OPN-c, thereby releasing a 5 kDa fragment that binds to CD44 receptors and induces hepatocellular carcinoma invasion and metastasis . (shu.edu)
In all, our study suggests that the expression of RANTES by breast tumor cells results not only in monocyte migration to the tumor site but also in protumorigenic activities of RANTES and of proinflammatory cytokines that may facilitate metastasis formation and contribute to disease progression. (nih.gov)
Thus, it is urgent to explore novel targets that may provide potential resolutions for metastasis in colorectal carcinoma cells. (biomedcentral.com)
Subsequently, we established in vitro migration and in vivo orthotopic metastatic mouse models to study the role of Rab3D in tumor metastasis. (oncotarget.com)
Increased expressions of Rab3D led to tumor invasion in vitro and lung metastasis in vivo , whereas Rab3D knockdown suppressed the tumor cell motility. (oncotarget.com)
Exosomes, characterized by a size of 30-100 nm in diameter, have been viewed as secreted vesicles that enable extracellular communications [ 4 - 6 ] and have played an important role in tumor progression and metastasis by promoting the extracellular matrix degradation and also by remodeling to establish the metastasis niche [ 7 , 8 ]. (oncotarget.com)
Cell migration and invasion play important roles in neoplastic metastasis. (koreascholar.com)
Emerging evidence has demonstrated that WISP2 is critically involved in cell proliferation, migration, invasion and metastasis in cancers. (biomedcentral.com)
Metastases represent the end products of a multistep cell-biological process termed the invasion metastasis cascade, which involves dissemination of cancer cells to anatomically distant organ sites. (medscape.com)
Infiltrated cells might proceed towards overt metastasis with or without an intervening period of latency (dormancy). (medscape.com)

Matrix metalloproteinases (MMPs) are a large family of mostly secreted, extracellularly acting proteolytic enzymes. (nature.com)
Matrix metalloproteinases (MMPs) are extracellularly acting enzymes that have long been known to have deleterious roles in brain injury and disease. (nature.com)
An inhibitor of matrix metalloproteinases (MMPs) decreased the transmigration of microglia in our system, indicating that MMPs play a role in microglial chemotaxis. (portlandpress.com)
For example, matrix metalloproteinases (MMPs), such as MMP-2 and −9, have been shown to be typical NF-κB target proteins that are responsible for extracellular matrix breakdown and cell invasion ( 7 ). (spandidos-publications.com)
Matrix metalloproteinases (MMPs) are enzymes degrading ECM. (lu.se)
Direct invasion by carcinoma cells of the stromal compartment involves active proteolysis effected principally by matrix metalloproteinases (MMPs), while degrading the BM and other ECM that lie in the path of invading tumor cells, MMP-expressing cells also liberate growth factors that are sequestered there, thereby fostering cancer cell proliferation. (medscape.com)

They also show that ECD cleavage is caused by matrix metalloproteinase 9 (MMP-9), whose inhibition leads to decreased NB cell migration. (dana-farber.org)
Kim JM, Noh EM, Kwon KB, et al: Curcumin suppresses the TPA-induced invasion through inhibition of PKCα-dependent MMP-expression in MCF-7 human breast cancer cells. (spandidos-publications.com)
Diallyl sulfide, diallyl disulfide, and diallyl trisulfide inhibit migration and invasion in human colon cancer colo 205 cells through the inhibition of matrix metalloproteinase-2, -7, and -9 expressions," Environ Toxicol. (doctorshealthpress.com)
Taken together, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by brazilin is mediated by the suppression of the NF-κB pathway in MCF-7 cells. (koreascholar.com)

Fibroblast-Derived Extracellular Vesicle-Packaged Long Noncoding RNA Upregulated in Diabetic Skin Enhances Keratinocyte MMP-9 Expression and Delays Diabetic Wound Healing. (nih.gov)
S1P or S1P analogs are likely to reach the binding pocket from within the cell membrane and not from the extracellular space, may be through an opening between helices I and VII. (wikipedia.org)
The proliferation of immune cells is due to S1P-mediated signals via the GTPase RAS and extracellular-signal regulated kinase (ERK). (wikipedia.org)
In a recent study published in Cell, first author, Hao Huang, PhD, and senior author, Rani George, MD, PhD , show that extracellular domain (ECD) cleavage of the ALK cell surface tyrosine kinase receptor mediates neuroblastoma cell migration through induction of an epithelial-to-mesenchymal transition (EMT) gene signature. (dana-farber.org)
OPN is encoded by the SPP1 gene , which is comprised of 7 exons and is located on the long arm of chromosome 4 near four other SIBLING (small integrin-binding N-linked glycoproteins) that are contained in bine and dentin - bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE). (shu.edu)
Data from the pharmacological studies using specific chemical inhibitors showed that activation of protein kinase C (PKC) and extracellular signal-regulated kinases (ERKs) was involved in TPA-induced migration/invasion, COX-2 protein expression, and MMP-9 activation. (tmu.edu.tw)
Remodelling of extracellular matrix (ECM) occurs in CRC. (lu.se)
In addition, it also regulated the extracellular secretion of Hsp90α to promote tumor cell migration and invasion. (oncotarget.com)
Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. (koreascholar.com)
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. (thermofisher.com)
The metastatic process involves sequential steps, including invasion through the surrounding extracellular matrix (ECM) and stromal cell layers, intravasation into the lumina of blood vessels, and survival in the circulation. (medscape.com)
One of the most basic features is cell invasion and movement through the extracellular matrix. (medscape.com)

Orthologous to human MMP9 (matrix metallopeptidase 9). (nih.gov)
Tumor necrosis factor-alpha, a major monocyte-derived cytokine, was found to promote the expression of MMP9 and MMP2 by MCF-7 and T47D breast adenocarcinoma cells, respectively, and to induce the de novo expression of an additional proteolytic enzyme by T47D cells, presumably MMP9. (nih.gov)
The possibility that RANTES may act directly on breast tumor cells was supported by detection of the expression of the CCR5 RANTES receptor in biopsy sections of breast cancer patients and by the ability of RANTES to promote the expression of MMP9 by MCF-7 cells. (nih.gov)
In breast cancer cells, 27-hydroxycholesterol has been found to increase matrix metalloproteinase 9 (MMP9) expression, activate signal transducer and activator of transcription 3 (STAT3), increase migration, invasion and epithelial-to-mesenchymal transition, as well as activate oncoprotein Myc by increasing its stability. (news-medical.net)
MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. (thermofisher.com)

The main functions of S1P-S1PR1 system are as follows: The phosphatidylinositol 3-kinase (PI3K) and the lipid dependent protein kinase B (PKB) signaling pathway increases the survival of lymphocytes and other immune cells by inhibiting apoptosis. (wikipedia.org)
It is reportedly involved in many physiological functions of cells, including apoptosis, proliferation, invasion and angiogenesis ( 5 ). (spandidos-publications.com)
Reference: Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via down regulation of matrix metalloproteinase-9. (pacherbs.com)
Knockdown of ENG inhibited cell viability, migration and invasion, and induced cell apoptosis in hEM15A cells. (viamedica.pl)
In the present study, we reported that celastrol potentiated tumor necrosis factor-α (TNF-α)-induced apoptosis, affected activation of caspase-8, caspase-3 and PARP cleavage, and inhibited the expression of anti-apoptotic proteins such as cellular inhibitor of apoptosis protein 1 and 2 (cIAP1 and cIAP2), cellular FLICE-inhibitory protein (FLIP), and B-cell lymphoma 2 (Bcl-2). (spandidos-publications.com)
Jin ML, Park SY, Kim YH, Park G and Lee SJ: Halofuginone induces the apoptosis of breast cancer cells and inhibits migration via downregulation of matrix metalloproteinase-9. (spandidos-publications.com)
In a retrospective study of 171 patients with NSCLC, OPN and thrombospondin-1 (a matrix protein that induces proliferating endothelial cells to secrete Fas ligand, which mediates apoptosis) levels were compared. (shu.edu)
Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. (biomedcentral.com)
SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. (biomedcentral.com)
In this paper, we verified the mRNA and protein expression of SDC-1 in human colorectal carcinoma tissues and focused on the biological cellular effects of SDC-1 on human colorectal carcinoma cell lines (SW620, SW480 and LOVO), by evaluating cell viability, apoptosis, migration and adhesion. (biomedcentral.com)
Cell proliferation was detected by MTT assay and apoptosis was measured by FACS in ESCC cells after WISP2 downregulation and overexpression. (biomedcentral.com)
WISP2 overexpression inhibited cell growth and induced cell apoptosis, suppressed cell migration and invasion in ESCC cells. (biomedcentral.com)
Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells. (asia.edu.tw)
Cancer is a complex disease in which many basic processes, such as cell division, apoptosis, and cell migration are dysregulated. (medscape.com)

Developmentally, it is well established that expression of the ID members is strongest in stem/progenitor cells, while their levels decrease upon differentiation. (nature.com)
Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. (neuroprobe.com)
Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells. (neuroprobe.com)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)
We examined the role of tissue plasminogen activator- (tPA-) matrix metalloproteinase- (MMP-) 9 in mobilizing endothelial progenitor cells (EPCs) from bone marrow to circulation and critical limb ischemia (CLI) region. (cell-metabolism.com)
Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. (thermofisher.com)

Furthermore, ATP promoted the migration of microglia but not macrophages across the MBEC monolayer. (portlandpress.com)
Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. (bu.edu)
The CCL17-CCR4 axis between endometrial stromal cells and macrophages contributes to the high levels of IL-6 in ectopic milieu. (endometriosi.it)
The aim of this study was to investigate whether the cross talk between endometrial stromal cells (ESCs) and macrophages contributes to the elevated IL-6 production. (endometriosi.it)
Macrophages secrete matrix metalloproteinase 9 (MMP-9), an enzyme that weakens the fibrous cap of atherosclerotic plaques, predisposing them to plaque rupture and subsequent ischemic events. (neuroprobe.com)
Tumour cells are able to recruit macrophages and change their behaviour. (lu.se)
M2 macrophages derived from human monocyte cell line THP-1 secreted high levels of IL-8 and leukotriene D4 (LTD4), which induced colon cancer cell migration, and Th2 cytokines (IL-4, IL-10) to maintain a tumoral immunosuppressive environment. (lu.se)
CD47 is involved in crosstalk between colon cancer cells and macrophages by interacting with its ligand signal-regulatory protein α on macrophages. (lu.se)

In addition, nuclear factor (NF)‑κB and matrix metalloproteinase (MMP) expression was examined by western blotting, and MMP‑2/‑9 activities were determined by gelatin zymography. (spandidos-publications.com)
To perform this function, DC are capable of capturing antigens, processing them, and presenting them on the cell surface complexed to major histocompatibility (MHC) molecules, for example, human leukocyte antigen- (HLA-) DR. Along with that, context-dependent expression of costimulatory molecules, such as CD80 and CD86, and secretion of cytokines occurs. (hindawi.com)
Western blot analysis and real-time PCR showed that celastrol dose-dependently suppressed TNF-α-induced MMP-9 gene expression at both the mRNA and protein levels in MDA-MB-231 cells. (spandidos-publications.com)
Lee SO, Jeong YJ, Yu MH, et al: Wogonin suppresses TNF-alpha-induced MMP-9 expression by blocking the NF-kappaB activation via MAPK signaling pathways in human aortic smooth muscle cells. (spandidos-publications.com)
Yan C and Boyd DD: Regulation of matrix metalloproteinase gene expression. (spandidos-publications.com)
Kim Y, Kang H, Jang SW and Ko J: Celastrol inhibits breast cancer cell invasion via suppression of NF-κB-mediated matrix metalloproteinase-9 expression. (spandidos-publications.com)
Ling H, Zhang Y, Ng KY and Chew EH: Pachymic acid impairs breast cancer cell invasion by suppressing nuclear factor-κB-dependent matrix metalloproteinase-9 expression. (spandidos-publications.com)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
Moreover, Al-positive samples exhibited a significant decrease in the number of apoptotic cells, as well as the expression of the anti-apoptotic molecule BCL-2. (bvsalud.org)
Western blot analysis and immunofluorescence experiments showed that PPI reduced the expression of GRP78, E-cadherin, N-cadherin, Vimentin, and ABCG2 in SR-HCC cells. (bvsalud.org)
Further examination revealed that PPI hindered the expression of GRP78 protein, resulting in a suppressive effect on SR-HCC cell migration and invasion. (bvsalud.org)
In-Cell Western assay was used for protein expression of CCL17 and phosphorylation levels of ERK, JNK, and P38. (endometriosi.it)
Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. (neuroprobe.com)
Independent of HIFα, hypoxia induces OPN expression in breast cancer cells, which leads to NFkB activation, VEGF expression , and angiogenesis. (shu.edu)
OPN induces the expression of oxidoreductases, which protects cells from anoikis during anchorage independent growth. (shu.edu)
In the present study, analysis of the regulation of RANTES expression demonstrates that the expression of RANTES in breast tumor cells is elevated significantly and in a synergistic manner by IFN-gamma and tumor necrosis factor-alpha. (nih.gov)
Moreover, RANTES promotes the expression of matrix metalloproteinase (MMP) 9 by THP-1 monocytic cells and elevates vascularity in chick chorioallantoic membrane assays. (nih.gov)
A dose- and time-dependent increase in cyclooxygenase-2 (COX-2) gene expression with elevated prostaglandin E 2 (PGE 2 ) production was identified in TPA- but not in 4α-TPA (a respective inactive compound)-treated U87 cells TPA-induced migration/invasion was significantly blocked by adding the COX-2-specific inhibitor, NS398, through a reduction in PGE 2 production. (tmu.edu.tw)
Stimulation of intracellular peroxide production by TPA was detected by a DCHF-DA assay, and the addition of superoxide dismutase (SOD) or tempol significantly inhibited TPA-induced migration/invasion and COX-2 protein expression accompanied by a decrease in peroxide production. (tmu.edu.tw)
CD47 binding to α2β1 integrin could regulate cyclooxygenase-2 expression and cell migration on collagen I surface in intestinal epithelial cells. (lu.se)
IL-8 and LTD4 could induce CD47 expression in CRC cells. (lu.se)
An increased expression of LDL receptor has also been found in breast cancer cells enriched with cholesteryl esters, which could be due to higher activity of acyl-coenzyme A: cholesterol acyltransferase. (news-medical.net)
Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). (biomedcentral.com)
Here, we reported that the expression levels of Rab3D were dysregulated in cancer cells and highly correlated with tumor malignancies in the clinical samples. (oncotarget.com)
Brazilin, an active component of sappan wood (Caesalpinia sappan), decreases TPA-induced MMP-9 expression and invasion in MCF-7 cells. (koreascholar.com)
By day 14 in animals with CLI expression levels of proangiogenic factors (CXCR4 SDF-1concentration accompanied by upregulation of MMP-9 activity in BM [7]. (cell-metabolism.com)
One study further identified that WISP2 expression was enhanced by serum and correlated with serum-induced cell proliferation in breast cancer cells, demonstrating that WISP2 could enhance cell proliferation in breast cancer [ 8 ]. (biomedcentral.com)
Gallic acid induces DNA damage and inhibits DNA repair-associated protein expression in human oral cancer SCC-4 cells. (asia.edu.tw)
Our laboratory focusses on how WNT5A expression is regulated in these cancers and deciphering the WNT5A signaling pathways governing cancer cell migration and invasion. (lu.se)
Interleukin-6 drives melanoma cell motility through p38α-MAPK-dependent up-regulation of WNT5A expression. (lu.se)
There are, in addition, other cals that are able to provoke an immune response such that characteristics, such as the ability to increase matrix allergic sensitization of the respiratory tract will be metalloproteinase expression or to cause perturbation of acquired. (cdc.gov)

In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking. (wikipedia.org)
S1PR1 activation is heavily involved in immune cell regulation and development. (wikipedia.org)
Yu HY, Kim KS, Moon HI, Kim KM, Lee YC and Lee JH: JNP3, a new compound, suppresses PMA-induced tumor cell invasion via NF-κB down regulation in MCF-7 breast cancer cells. (spandidos-publications.com)
Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
Q2986 Kesanakurti,D., Chetty,C., Maddirela,D.R., Gujrati,M., Rao,J.S. Essential role of cooperative NF-kappaB and Stat3 recruitment to ICAM-1 intronic consensus elements in the regulation of radiation-induced invasion and migration in glioma. (alzet.com)
NF-κB is transcription factor important in the regulation of MMP-9, as the promoter of MMP-9 gene contains binding sites for NF-κB. (koreascholar.com)
Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)
However although these studies [5-8] consistently emphasized the relationship between MMP-9 activity and the mobilization of EPCs from BM to circulation the precise mechanisms involved in the regulation of the kinetics of EPC mobilization have not been thoroughly clarified in these studies [5-8]. (cell-metabolism.com)
To further understand whether MMP-9 plays a unique role in tPA-mediated regulation of the mobilization Olmesartan of BM-EPCs into circulation the MMP-9 deficient mice (MMP-9? (cell-metabolism.com)
Mechanistically, WISP2 exerts its tumor suppressive functions via regulation of ERK1/2, Slug, and E-cadherin in ESCC cells. (biomedcentral.com)
An up-regulation of several key genes known to be important in cell invasion, notably matrix metalloproteinase-2 (MMP-2), was observed in the exposed mesothelial cells as determined by real-time PCR. (cdc.gov)
It has been suggested that endogenous factors might contribute to the rapid progression of periodontal breakdown such as inappropriate regulation of enzymes, lipid mediators, collagen biosynthesis or T cell immunodeficiency. (bvsalud.org)
Hypoxia leads to the up-regulation of hypoxia-induced transcription factors (HIF)-a and HIF-2a, which are the master regulators of proangiogenic signals, mainly the vascular endothelial cell growth factors (VEGFs). (medscape.com)

Phosphoinositide 3-kinase (PI3K) and the GTPase RAC are responsible of the lymphocytes migration and their interactions with other cells or with connective-tissue surfaces. (wikipedia.org)
Park SK, Hwang YS, Park KK, Park HJ, Seo JY and Chung WY: Kalopanaxsaponin A inhibits PMA-induced invasion by reducing matrix metalloproteinase-9 via PI3K/Akt- and PKCdelta-mediated signaling in MCF-7 human breast cancer cells. (spandidos-publications.com)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)

S1PR1 is involved in the motility of cancer cells upon stimulation by S1P. (wikipedia.org)
In addition, decreased production of the inflammatory mediators, TNF- α and MMP-9, upon Toll-like receptor (TLR) stimulation was found at the end of the exercise program. (hindawi.com)
In addition, celastrol significantly reduced the invasion of MDA-MB-231 human breast cancer cells after TNF-α stimulation. (spandidos-publications.com)
In the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulation, an increase in the migration/invasion of U87 glioblastoma cells was detected by a wound healing assay, transwell analysis, and spheroid formation assay by inducing matrix metalloproteinase-9 (MMP-9) enzyme activity via a gelatin zymographic analysis. (tmu.edu.tw)
The enhanced levels of MMP-7 and -9 mRNA expressions and increased cell invasion into matrigel were observed in colon cancer cells upon stimulation with TAM-conditioned medium. (lu.se)

Woessner JF Jr: Matrix metalloproteinases and their inhibitors in connective tissue remodeling. (spandidos-publications.com)

To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
Our in vitro studies included scratch wound-healing migration assays and transwell assays to examine PPI's effect on HCC cell migration and invasion. (bvsalud.org)
The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. (endometriosi.it)
at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference. (endometriosi.it)
The RealStar Filovirus Display screen reverse transcription-polymerase chain response (RT-PCR) equipment and the derived RealStar Zaire Ebolavirus RT-PCR equipment had been validated utilizing in vitro transcripts, supernatant of contaminated cell cultures, and medical specimens from sufferers with EVD. (sebov-detection.com)
Ranunculaceae) was found to possess significant in vitro anti-proliferative effects on MCF-7 breast cancer cells. (bvsalud.org)
Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)

In vascular endothelial cells the binding of S1P to S1PR1 induces migration, proliferation, cell survival and morphogenesis into capillary-like structures. (wikipedia.org)
Chronic exposure to carbon nanotube s induces invasion of human mesothelial cells through matrix metalloproteinase-2. (cdc.gov)

Like the other members of the GPCR family, S1PR1 senses its ligand from outside the cell and activates intracellular signal pathways that at last lead to cellular responses. (wikipedia.org)
In the current study, the anti‑invasive activities and associated signaling pathways of celastrol were determined in ovarian cancer cells. (spandidos-publications.com)
In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. (biomedcentral.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In the tumor microenvironment, cell migration and invasion are regulated by many factors such as intracellular signaling pathways, growth factors or onco-proteins [ 2 ]. (oncotarget.com)

In addition to EPC the role of MMP-9 in regulating migration of neuronal and mesenchymal stem cells has also been indicated through cultured cell models and gene deficient mouse model [13 14 A recent study also indicated that this levels and activity of MMP-9 were altered in patients with CLI [15]. (cell-metabolism.com)

Cell proliferation was tested by MTT assay. (spandidos-publications.com)
Enzyme-linked immunosorbent assay (ELISA) was for IL-6 levels in peritoneal fluid and cell culture supernatant. (endometriosi.it)
A phytochemical investigation using assay-guided fractionation of the ethanolic extract of C. dahurica resulted in the isolation of one new phenolic amide glycoside 3, two new lignan glycosides 4 and 7, one new 9,19-cycloartane triterpenoid glycoside 6, and thirteen known constituents 1, 2, 5, and 8â »17. (bvsalud.org)
Cell migration and invasion were analyzed by wound healing assay and transwell migration assay, respectively. (biomedcentral.com)

The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
Protein kinase C regulates human pluripotent stem cell self-renewal. (rndsystems.com)
Disruption of this p130Cas complex by small interfering RNA silencing inhibits p44/42 mitogen-activated protein kinase phosphorylation and microglial migration, illustrating the importance of this pathway in microglial activation and recruitment. (neuroprobe.com)
Simvastatin dose dependently inhibited THP-1 cell migration mediated by monocyte chemoattractant protein 1, with a 50% inhibitory concentration of about 50 nM. (neuroprobe.com)
We detected protein levels of Rab3D in nine cancer cell lines and twelve types of clinical cancer specimens. (oncotarget.com)

LTP-associated MMP proteolysis is probably then terminated by an increase in the activity of endogenous inhibitors called tissue inhibitors of metalloproteinases. (nature.com)
Burk J, Burggraf D, Vosko M, Dichgans M, Hamann G. Hypothermia strengths inhibitors of matrix degrading proteases following experimental focal cerebral ischemia in mice. (mpg.de)

With regard to cancer treatment, celastrol has been shown to exert considerable cell-killing effects and to shrink xenografted tumors in tissue and animal models of various malignancies including cancers of the prostate ( 16 - 18 ), breast ( 19 ), liver ( 20 , 21 ) and lung ( 22 , 23 ). (spandidos-publications.com)
These chemicals do prevent cancer cell growth directly but they also can block estrogen receptors in breast tissue reducing the risk of excessive cellular growth propagated by estrogen. (doctorshealthpress.com)
Glial cell line-derived neurotrophic factor family receptor alpha (GFRα) members have been widely connected to the mechanisms contributing to cell growth, differentiation, cell migration and tissue maturation. (medsci.org)
Based on whether it cooperates with the second receptor RET, GFRα has also been widely linked to the mechanisms that contribute to cell growth, differentiation and migration and tissue maturation. (medsci.org)

To evaluate whether statins influence the proinflammatory responses of monocytic cells, we studied their effects on the chemotactic migration and MMP-9 secretion of human monocytic cell line THP-1. (neuroprobe.com)

FoxO4 Regulates Tumor Necrosis Factor Alpha-Directed Smooth Muscle Cell Migration by Activating Matrix Metalloproteinase 9 Gene Transcription. (knu.ac.kr)
Whole genome microarray analysis further indicated the importance of MMP-2 in the invasion gene signaling network of the exposed cells. (cdc.gov)
Knockdown of MMP-2 in CNT and asbestos-exposed cells by shRNA-mediated gene silencing effectively inhibited the aggressive phenotypes. (cdc.gov)

Cell migration was detected by wound healing and Transwell assays, while cell invasion was detected by a Matrigel‑coated Transwell method. (spandidos-publications.com)

Abundant proteins, including membrane trafficking molecules, cytoskeleton molecules, chaperones, signal transduction proteins and cytoplasmic enzymes have been included in secreted exosomes [ 9 ]. (oncotarget.com)

The MYC proto-oncogene ( MYC ) is one of the most frequently overexpressed genes in breast cancer that drives cancer stem cell-like traits, resulting in aggressive disease progression and poor prognosis. (nature.com)
Tumor initiation and progression often involve the dysregulation of developmentally important genes in cancer cells, endowing them with stem cell-like features such as enhanced self-renewal, invasiveness, and an aberrant differentiation state [ 1 ]. (nature.com)
Recent evidence also suggests involvement of innate immunity, including dendritic cells (DC), in the initiation and maintenance as well as progression of MS [ 11 ]. (hindawi.com)
EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
We investigated the presence of aluminium (Al) in human colon cancer samples and its potential association with biological processes involved in cancer progression, such as epithelial to mesenchymal transition (EMT) and cell death. (bvsalud.org)
Breast cancer progression may be affected by various cellular components expressed by the tumor cells and/or by microenvironmental factors. (nih.gov)
We have identified recently the CC chemokine regulated on activation, normal T cell expressed and secreted (RANTES), a major monocyte chemoattractant expressed by breast tumor cells, as a potential contributor to breast cancer progression. (nih.gov)
Identification of the mechanisms by which RANTES may contribute to breast cancer progression included the analysis of the potential ability of RANTES to act in paracrine and indirect mechanisms, as well as directly on the tumor cells, to promote disease progression. (nih.gov)
Our results suggest that breast tumor cell-derived RANTES may promote breast cancer progression by its partial contribution to monocyte migration into breast tumor sites. (nih.gov)
In estrogen receptor-positive and triple-negative breast cancer cells, oncosterone increases cellular proliferation and promotes cancer progression. (news-medical.net)

S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. (wikipedia.org)
In conclusion, we offer evidence that microglia migration across the brain endothelial cell monolayer is increased in the presence of ATP in a manner that involves MMP secretion. (portlandpress.com)
Further, hypoxia and estrogen are interchangeable as both similarly modulate epithelial-endothelial cell interaction. (biomedcentral.com)

In the brain, they have well-described roles in slowly emerging, but long-lasting pathophysiological processes of cell loss and synaptic dysfunction associated with acute injury, ischaemia, neurodegeneration and demyelination. (nature.com)
Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization. (bu.edu)
Decock J, Thirkettle S, Wagstaff L and Edwards DR: Matrix metalloproteinases: protective roles in cancer. (spandidos-publications.com)

These cells include dermal resident dendritic cells (DC), epidermal Langerhans cells, neutrophils and monocytes, which are released from the blood when the dermal capillary is disrupted and a reputed blood lake is formed [ 3 - 6 ]. (biomedcentral.com)
These data supported the role of geranylgeranylation in the migration and MMP-9 secretion of monocytes. (neuroprobe.com)

Transmission electron microscopy and energy-dispersive X-ray microanalysis confirmed the presence of Al specifically in intracytoplasmic electrondense nanodeposits adjacent to mitochondria of colon cancer cells. (bvsalud.org)
CD47, as a key regulator of crosstalk between colon cancer cells and TAMs, could thus be a therapeutic target for CRC. (lu.se)

2.2 Animals Irradiation and Bone Marrow Cell (BMC) Transplantation MMP-9 deficient mice in C57BL/6J background were purchased from The Jackson Laboratory (= 6 for each subgroup). (cell-metabolism.com)
Although tumor-associated angiogenesis has traditionally been defined as the sprouting of new vessels from preexisting vessels, it is becoming clear that the blood vessels that support tumor growth can also originate from cells recruited from the bone marrow or can even differentiate from tumor stem cells (vascular mimicry). (medscape.com)

Once formed, the complex translocates to the nucleus where it acts as a transcription factor by binding to the estrogen response elements (EREs) at the promoters of estrogen responsive genes[ 9 , 10 ]. (biomedcentral.com)

Global transcriptome and chromatin occupancy analyses of ZNF148 revealed a central role in inhibiting cancer cell de-differentiation and migration. (nature.com)
The MYC proto-oncogene is a transcription factor essential in stem/progenitor cell maintenance and differentiation [ 11 ]. (nature.com)
DC are professional antigen-presenting cells with the unique capacity to polarize the differentiation of T cells, thereby regulating the balance between inflammation and tolerance [ 12 ]. (hindawi.com)
The glial cell line-derived neurotrophic factors (GDNFs), a family of neurotrophic factors, were initially thought to be able to regulate the growth, survival, and differentiation of neural-derived cell types. (medsci.org)
This cholesterol metabolite can act as a tumor suppressor by promoting cellular differentiation and cancer cell death. (news-medical.net)
Fascinatingly some previous studies have identified that MMP-9 acts by cleaving the membrane-bound Olmesartan c-Kit ligand (c-Kit-L) to the soluble form c-Kit-L which then interacts with the EPCs c-Kit receptor to initiate the signal (i.e. the downstream signaling of MMP-9) that is crucial for BM-EPC differentiation and mobilization to systemic circulation [9-12]. (cell-metabolism.com)

Additionally, the natural flavonoids quercetin (QE), baicalein (BE), and myricetin (ME) effectively blocked TPA-induced migration/invasion while simultaneously inhibiting COX-2/PGE 2 production, MMP-9 enzyme activity, and peroxide production in U87 cells. (tmu.edu.tw)

It also inhibited bacterial lipopolysaccharide-stimulated secretion of MMP-9. (neuroprobe.com)

The ethanolic (EtOH) extract and several isolated compounds exert the inflammatory effect of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cells. (bvsalud.org)
By using a tPA knockout mouse model we have further identified the essential role of endogenous tPA in augmenting circulating EPCs angiogenesis and blood flow through regulating MMP-9 activity in the ischemic limb in a murine model [8]. (cell-metabolism.com)

We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)

ZNF148 suppressed cell proliferation and migration and was transcriptionally repressed by MYC in breast cancer. (nature.com)
Depletion of ZNF148 by short hairpin RNA (shRNA) and CRISPR/Cas9 increased triple-negative breast cancer (TNBC) cell proliferation and migration. (nature.com)
LDL has also been found to stimulate the proliferation and migration of breast cancer cells. (news-medical.net)

In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
In 27 patients with NSCLC (non-small cell lung cancer), OPN levels at the completion of radiation, as well as the change in plasma concentration during therapy, was predictive of overall survival. (shu.edu)

Since recent evidence supports a crucial role of dendritic cells (DC) in the pathogenesis of MS, we investigated the effect of a 12-week combined exercise program in MS patients on the number and function of DC. (hindawi.com)
Coincubation with Leishmania changes the migratory pattern of dendritic cells in vivo . (biomedcentral.com)

Therefore, we aim to explore the effects and the potential mechanism of WISP2 on proliferation and motility and invasion of ESCC cells. (biomedcentral.com)

Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)

In the present study we identify the focal adhesion-associated proteins p130Cas, Pyk2, and paxillin as novel members of the tyrosine kinase signaling pathway downstream of CD36 and show that assembly of this complex is essential for microglial migration. (neuroprobe.com)
Together, these data are the first to identify the signaling cascade that directly links CD36 to the actin cytoskeleton and, thus, implicates it in diverse processes such as cellular migration, adhesion, and phagocytosis. (neuroprobe.com)

These findings uncover a previously unknown tumor suppressor role for ZNF148, and a transcriptional regulatory circuitry encompassing MYC, ZNF148, and ID1/3 in driving cancer stem cell traits in aggressive breast cancer. (nature.com)
Consequently, in order to develop effective targeted therapy, there is a pressing need to identify all molecular regulators that define cancer stem cell traits in TNBC. (nature.com)
We previously reported a MYC-centered regulatory network in mouse embryonic stem (ES) cells that accounts for the similarity between the ES cells and cancer cells, and is associated with a worse prognosis in cancer, including cancers of the breast [ 20 ]. (nature.com)
Despite ZNF148 being an established node of the MYC-network in ES cells, and the significance of MYC in driving cancer stem cells traits, the role of ZNF148 in breast cancer remains elusive. (nature.com)
Stem cell senescence causes different complications. (magiran.com)
In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
This manuscript examines protective medicines and supplements which are capable of hindering stem cell senescence. (magiran.com)
Breast cancer stem cells (BCSCs) are one of the main factors driving chemoresistance, thereby contributing to poor prognosis and clinical outcomes [ 3 - 5 ]. (hindawi.com)
Dr. Elliott J. Hagedorn is a cell and developmental biologist with expertise in hematopoiesis, stem cell biology and vascular heterogeneity. (bu.edu)
Transcription factor induction of vascular blood stem cell niches in vivo. (bu.edu)
Stem Cell Reports. (bu.edu)
Helps maintain human pluripotent stem cells in undifferentiated state. (rndsystems.com)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
The GDNF family ligands (GFLs) function through a glycosyl-phosphatidylinositol-(GPI) anchored coreceptor, GDNF family receptor alpha (GFRα), and rearranged during transfection (RET), a well-known receptor tyrosine kinase involved in kidney development, spermatogonial stem cell maintenance, and the development and maintenance of the sympathetic, parasympathetic, and enteric nervous systems [ 1 , 2 ]. (medsci.org)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)

We have previously examined a well characterized this research non transfected neuronal cell culture model that expresses three known mERs mER, mER, and GPR30. (inhibitorkit.com)

Zhang S, Li Z, Wu X, Huang Q, Shen HM and Ong CN: Methyl-3-indolylacetate inhibits cancer cell invasion by targeting the MEK1/2-ERK1/2 signaling pathway. (spandidos-publications.com)
In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)

An increase in NADPH oxidase activity by TPA was examined, and TPA-induced migration/invasion was blocked by adding DPI, an NADPH oxidase inhibitor. (tmu.edu.tw)

Anatomy38

Organisms7

Diseases16

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Psychiatry and Psychology1

Phenomena and Processes43

Disciplines and Occupations1

Information Science3

Metastasis20

MMPs6

Inhibition4

Extracellular12

MMP95

Apoptosis14

Progenitor cells7

Macrophages8

Expression31

Regulation14

PI3K3

Stimulation5

Connective-tissue1

Vitro8

Induces2

Pathways5

Mesenchymal stem1

Assay4

Protein5

Inhibitors2

Tissue4

Monocytic cells1

Gene3

Transwell1

Enzymes1

Progression11

Endothelial cell3

Roles3

Monocytes2

Colon cancer cells2

Bone marrow2

Genes1

Differentiation6

Enzyme1

Secretion1

Murine2

20201

Proliferation and migration3

Survival2

Dendritic cells2

Motility1

Epithelial-mesenchyma1

Adhesion2

Stem15

Neuronal1

Pathway2

Inhibitor1