• The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88. (lsbio.com)
  • In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) TLR6 is observed, 5% cross-reactivity with recombinant human (rh) TLR1 and rmTLR2 is observed, and less than 2% cross-reactivity with rhTLR3 and rhTLR4 is observed. (bio-techne.com)
  • Monoclonal antibody 6C2 reacts with mouse Toll-like receptor 2 (TLR2, CD282). (hycultbiotech.com)
  • The present invention relates to methods for isolating the sequences of an antibody that reacts with a disease related antigen, e.g., an autoantigen, without knowing the identity of the antigen (sequence or structural epitope) a priori. (justia.com)
  • The antibody MEM-143 reacts with a protein determinant of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). (lsbio.com)
  • Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. (thermofisher.com)
  • Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)
  • After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. (lsbio.com)
  • Measurements have also been made on antibody conjugates. (2spi.com)
  • Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TOMM20. (abbkine.com)
  • OR2T4 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (antibodies-online.com)
  • We focus on developing monoclonal and polyclonal antibodies that are unique in binding a new epitope or in recognizing a new target. (abbiotec.com)
  • The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. (enzolifesciences.com)
  • The functional maturation of dormant pro-proteins is catalyzed by the proprotein convertase subtilisin/kexin (PCSK) enzymes (PCSK1-2, FURIN, PCSK5-7, membrane-bound transcription factor site 1, PCSK9) [ 1 ]. (oncotarget.com)
  • The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. (bio-techne.com)
  • Special Offer: Buy 2 proteins and get the third protein free. (origene.com)
  • IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (AENPEYLGLDVPV) corresponding to amino acids 1243-1255 of human c-erbB-2/HER-2/neu oncoprotein. (australbiologicals.com)
  • TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. (prosci-inc.com)
  • TOMM20L Belongs to the Tom20 family.Tom20 is the master receptor for protein import into mitochondria. (abbkine.com)
  • CD45RB is a splice isoform of CD45 (also called protein tyrosine phosphatase, receptor type, C), a glycoprotein receptor that is required for T-cell activation. (lsbio.com)
  • This polyclonal antibody can be used in ELISA (1: 100 dilution), immunocytochemistry (1: 100 dilution) and it recognizes c-erbB-2 protein (185 kDa) in Western Blot of HeLa cell lysate (1: 500 dilution). (australbiologicals.com)
  • This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. (origene.com)
  • The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. (origene.com)
  • In this study, we investigated the adjuvant activities of combinations of Toll-like receptor 9 (TLR9) and stimulator of interferon genes (STING) agonists in a SARS-CoV-2 receptor binding domain protein vaccine. (ijbs.com)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of IL-1 R6 extracellular domain (also called IL-1 Rrp2). (reliatech.de)
  • IL-1 Rrp2, also known as IL-1 R6, is a transmembrane protein belonging to the IL-1 receptor family. (reliatech.de)
  • Furthermore, gene co-expression analysis and further experiments showed that GBP2b regulates M1 polarization through activation of Toll-like receptor 4 and nuclear factor-κBp65 protein. (researchsquare.com)
  • Active immunization by vaccines against SARS-CoV-2 spike protein provides good protection from a severe course of COVID-19. (hilarispublisher.com)
  • G protein-coupled receptor 78 (GPR78) is an orphan GPCR that shares sequence homology with GPR26, and is widely expressed in selective regions of the brain. (kxcdn.com)
  • Development of antagonistic mAbs that specifically target the immune checkpoint receptor, programmed cell death protein-1 (PD-1), is of great interest for cancer immunotherapy. (bvsalud.org)
  • Caffeine, one of the most consumed central nervous system (CNS) stimulants, is an antagonist of A 1 and A 2A adenosine receptors. (frontiersin.org)
  • Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172). (qedbio.com)
  • In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. (hycultbiotech.com)
  • CD155, known as poliovirus receptor (PVR) or nectin-like 5, is a 70 kD type I transmembrane glycoprotein. (biolegend.com)
  • CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). (rndsystems.com)
  • The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). (rndsystems.com)
  • Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. (hycultbiotech.com)
  • These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). (invivogen.com)
  • TRPM7 (Transient receptor potential cation channel, subfamily M, member 7) is a divalent cation channel permeable to calcium and magnesium. (neuromics.com)
  • NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. (thermofisher.com)
  • After removing any unbound substances, a biotin-conjugated antibody specific for TOMM20 is added to the wells. (abbkine.com)
  • Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 0.5mg/ml. (angioproteomie.com)
  • There are at least twelve mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). (bio-techne.com)
  • If sensitivity is poor, it may be worth examining antibodies from other host species, as each will have different characteristics. (2spi.com)
  • TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. (bio-techne.com)
  • Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. (invivogen.com)
  • Full length recombinant human MD-2. (enzolifesciences.com)
  • Western blot analysis of MD-2 in human (A), mouse (B) and rat (C) spleen lysate using PAb to MD-2 (Prod. (enzolifesciences.com)
  • Immunohistochemical staining of normal human brain using PAb to MD-2 (Prod. (enzolifesciences.com)
  • TLR3 Antibody is a high quality research product used as Highly pure polyclonal antibody specific to human TRL3. (moleculardepot.com)
  • Highly pure polyclonal antibody specific to human TRL3. (moleculardepot.com)
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19811-1-AP (TLR4 Antibody) at dilution of 1:200 (under 40x lens). (ptglab.com)
  • TNF Alpha antibody LS-B2123 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. (lsbio.com)
  • Anti-TNF Alpha antibody IHC of human lung. (lsbio.com)
  • The overexpression of the erbB-2 proto-oncogene is associated with an aggressive human adenocarcinomas, mainly breast and ovarian tumours. (australbiologicals.com)
  • Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. (invivogen.com)
  • Anti-GPR78 antibody IHC of human placenta, villi. (kxcdn.com)
  • Anti-GPR78 antibody IHC of human Skin, Melanoma. (kxcdn.com)
  • We show that sasanlimab binds selectively and with similar high potency to human and cynomolgus monkey PD-1 receptor and blocks its interaction with PD-L1 and PD-L2, with no detectable Fc-dependent effector function. (bvsalud.org)
  • Similar to the human gut microbiome, >99% of the cataloged genes are bacterial. (bvsalud.org)
  • What sets this assay apart is its utilization of a distinctive neo-epitope capture antibody that selectively targets a specific C3d region of the alpha chain, which is not available in other C3 variants. (hycultbiotech.com)
  • The adaptive immune response produces a diversity of different antibodies (on the order of 10 11 unique sequences in a single individual), each of which is capable of binding to a different target epitope. (justia.com)
  • The isolation of antibodies that bind to a specific target, let alone a specific epitope on a known target, is of high interest for the development of new products in therapeutics, diagnostics, and more generally, for research purposes. (justia.com)
  • A much more challenging problem in antibody discovery is presented when it is known, or suspected, that an antibody response is present and is biologically relevant, but the identity of the target (and consequently, target epitope) is not known, and could be a single target selected from hundreds to tens of thousands of different targets. (justia.com)
  • As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. (biolegend.com)
  • FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. (thermofisher.com)
  • This Rat Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM20 in samples. (abbkine.com)
  • It is a ligand binding subunit that associates with IL-1 RAcP to form a functional receptor complex for IL-1F6, IL-1F8, and IL-1F9. (reliatech.de)
  • Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. (avivasysbio.com)
  • What buffer format is "Tnfrsf9 Antibody (OASA00035)" provided in? (avivasysbio.com)
  • This Mouse TLR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-102 amino acids from the N-terminal region of mouse TLR5. (prosci-inc.com)
  • IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. (angioproteomie.com)
  • TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of gram-negative bacteria, several whole gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. (hycultbiotech.com)
  • The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. (hycultbiotech.com)
  • It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). (rndsystems.com)
  • This antibody can be used for the neutralization of hTLR6, it blocks cellular activation induced by agonists that are recognized by TLR6 and TLR2, such as FSL-1 . (invivogen.com)
  • TLR6 co-operates with TLR2 to recognize diacylated lipoproteins (LPs) found in mycoplasma but is not required in the recognition of triacylated LPs found in bacteria [1, 2]. (invivogen.com)
  • TLR6 collaboration with TLR2 is essential for the recognition and signal transduction of diacylated LPs, such as MALP- 2 and FSL-1 [2, 3], as well as heat-killed mycoplasma, indicating that TLR6 and TLR2 play a central role in recognizing mycoplasma [2]. (invivogen.com)
  • In general, the SPI-Mark Colloidal Gold products, like all other colloidal gold products (to our knowledge) are that deep "Cabernet Sauvignon" red or almost purple color except for the SPI-Mark 2 nm gold products. (2spi.com)
  • mouse liver tissue were subjected to SDS PAGE followed by western blot with 19811-1-AP (TLR4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Browse our catalog for rabbit, mouse and rat antibodies that are developed for ELISA, western blot, immunoprecipitation, IHC and flow cytometry applications. (abbiotec.com)
  • Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). (thermofisher.com)
  • Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. (thermofisher.com)
  • We established a catalog of the mouse gut metagenome comprising â ¼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. (bvsalud.org)
  • Macrophages are the main source of IL-10, and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-¿B dependent), tumor necrosis factor TNF-a (via TNF receptor p55, NF-¿B-dependent), catecholamines, and IL-1. (angioproteomie.com)
  • 6: Schau I, Michen S, Hagstotz A, Janke A, Schackert G, Appelhans D, Temme A. Targeted delivery of TLR3 agonist to tumor cells with single chain antibody fragment-conjugated nanoparticles induces type I-interferon response and apoptosis. (moleculardepot.com)
  • doi: 10.1038/icb.1997.68;Eur J Immunol 1995 25(2):317. (lsbio.com)
  • C-erbB-2 oncoprotein is a 185 kDa type I membrane tyrosine kinase receptor and belongs to the EGF receptor family. (australbiologicals.com)
  • The monoclonal antibody 6C2 can be used for flow cytometry and immuno precipitation. (hycultbiotech.com)
  • The antibody was purified by affinity chromatography. (angioproteomie.com)
  • The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. (biolegend.com)
  • In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15). (rndsystems.com)
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution. (bio-techne.com)
  • In diseases and conditions in which autoantibodies are generated (e.g., autoimmune disease, cancer), the adaptive immune system escapes "tolerance", the internal checks and balances that would normally prevent the generation of antibodies that bind to self-antigens, and results in chronic inflammation, fibrosis, progressive tissue damage or a combination of the foregoing. (justia.com)
  • For example, there is great interest in using colloidal gold tagged antibodies for identifying surface antigens on the surfaces of cultured cells. (2spi.com)
  • Cathepsin K antibodies are marketed for research into expression of this enzyme by various cells. (wikipedia.org)
  • The antibody works in immunofluorescence of fixed RAW cells. (hycultbiotech.com)
  • The method entails optionally subjecting a sample to conditions suitable for expansion of one or more of the lymphocytes to form an expanded sample comprising antibody secreting cells (ASCs) and distributing the sample into containers to provide a plurality of subsamples. (justia.com)
  • CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). (lsbio.com)
  • It has also been reported in blocking of IgE to Fc epsilon Receptor and degranulation of mast cells. (thermofisher.com)
  • The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. (thermofisher.com)
  • For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. (thermofisher.com)
  • Moreover, it easily enters cells and binds to toll-like receptor 4. (nature.com)
  • In dendritic cells, CpG-2722 and 2'3'-c-di-AM(PS)2 showed a cooperative effect on inducing expression of molecules critical for T cell activation. (ijbs.com)
  • Gene expression signatures within memory B cells suggest qualitative differences in the antibody responses in aged patients with severe disease. (medrxiv.org)
  • CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. (biolegend.com)
  • Their serious social impact is clear from the ongoing COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which began in late 2019. (ijbs.com)
  • Coronavirus disease-2019 (COVID-19), caused by the respiratory virus severe acute respiratory syndrome coronavirus (SARS-CoV-2), has resulted in a global pandemic with over 16 million cases and 300,000 deaths in the U.S. alone. (medrxiv.org)
  • When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. (thermofisher.com)
  • The exact mechanism by which GAG accumulation leads to disease features is unknown but may involve interference in cellular trafficking of molecules, alteration of the extracellular matrix, and interference with cell signaling and cell receptor functions. (medscape.com)