• Additionally, the use of simvastatin plus classII histone deacetylase (HDAC) inhibitor (MC1568) induced further overexpression of p27(KIP1) by inhibiting HDAC5 induction originated from downregulated EZH2 in CRC cells and synergistically led to considerable antiproliferative effects. (nih.gov)
  • Canonical Notch signaling activates the transcription of BMI1 proto‑oncogene polycomb ring finger, cyclin D1, CD44, cyclin dependent kinase inhibitor 1A, hes family bHLH transcription factor 1, hes related family bHLH transcription factor with YRPW motif 1, MYC, NOTCH3, RE1 silencing transcription factor and transcription factor 7 in a cellular context‑dependent manner, while non‑canonical Notch signaling activates NF‑κB and Rac family small GTPase 1. (spandidos-publications.com)
  • Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). (neobiotechnologies.com)
  • Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. (neobiotechnologies.com)
  • The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. (affbiotech.cn)
  • CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. (rockland.com)
  • CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. (rockland.com)
  • CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). (rockland.com)
  • CDK2 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research. (rockland.com)
  • Recombinant full-length human CDK2 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. (rockland.com)
  • CDK2 Protein is suitable for use in Western Blot. (rockland.com)
  • p27 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclin E-CDK2, which phosphorylates pRb, thereby ushering the cell from G1 into S phase through the Restriction point (Figure 2). (shu.edu)
  • The cyclin D1-Cdk4 complex phosphorylates the pRB protein leading to sequential phosphorylation by cyclin E-Cdk2 and release of free E2F. (shu.edu)
  • This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. (affbiotech.cn)
  • Because of the clinical similarity between MEN 1 and MEN 4, patients with this constellation of symptoms should undergo genetic testing utilizing a panel of genes that includes both MEN1 and CDKN1B . (merckmanuals.com)
  • Assessment of p27 (cyclin-dependent kinase inhibitor 1B) and aryl hydrocarbon receptor-interacting protein (AIP) genes in multiple endocrine neoplasia (MEN1) syndrome patients without any detectable MEN1 gene mutations. (cdc.gov)
  • Mutation analysis of MEN1, HRPT2, CASR, CDKN1B, and AIP genes in primary hyperparathyroidism patients with features of genetic predisposition. (cdc.gov)
  • Genetic studies have clarified that most microcephaly genes encode ubiquitous proteins involved in mitosis and in maintenance of genomic stability, but the effects of their inactivation are particularly strong in neural progenitors. (cancerindex.org)
  • For example, when p27 is held (sequestered) in the fluid that surrounds the nucleus (the cytoplasm) instead of being transported into the nucleus, the protein is unavailable to block cell cycle progression. (medlineplus.gov)
  • The proper development and homeostasis of tissues and organs at the cellular level are ensured by a finely and timely regulated progression of the cell division cycle, which requires the perfectly harmonized activity of numerous protein kinases/phosphatases and regulatory proteins. (mdpi.com)
  • Both proteins may play a role in UC progression and can have a value as prognostic and/or diagnostic markers of UC, as well as targeted therapies. (springeropen.com)
  • Survivin belongs to the family of inhibitors of apoptosis proteins (IAPs) that plays a pivotal role in mitotic progression, apoptosis inhibition, angiogenesis, progression of cancer, and invasion [ 5 ]. (springeropen.com)
  • The interactions of cyclins, cyclin-dependent kinases (CDKs), and their inhibitors regulate cell cycle progression. (springeropen.com)
  • Browse our antibodies, ELISA kits and proteins related to mitotic G1-G1/S phases. (antibodies-online.com)
  • Moreover, PD-1 and PD-L1 inhibitors are being tested in combination with other checkpoint inhibitors, targeted therapies, cancer vaccines, monoclonal antibodies, and other modalities. (shu.edu)
  • Small-molecule inhibitors, antagonistic monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), bispecific antibodies or biologics (bsAbs) and chimeric antigen receptor-modified T cells (CAR-Ts) targeting Notch signaling components have been developed as investigational anti-cancer drugs ( 10 - 12 ). (spandidos-publications.com)
  • At least eight mutations in the CDKN1B gene have been found to cause a relatively rare form of multiple endocrine neoplasia called type 4. (medlineplus.gov)
  • Most of the CDKN1B gene mutations that cause multiple endocrine neoplasia type 4 change single protein building blocks (amino acids) in the p27 protein. (medlineplus.gov)
  • Apart from multiple endocrine neoplasia, the CDKN1B gene is rarely mutated in cancerous tumors. (medlineplus.gov)
  • Germline CDKN1B/p27Kip1 mutation in multiple endocrine neoplasia. (cdc.gov)
  • Moreover, we discussed the clinical correlation between MDSCs and the diagnosis of malignant hematological disease, as well as the drugs targeting MDSCs, and focused on summarizing the therapeutic strategies in combination with other immunotherapies, such as various immune checkpoint inhibitors (ICIs), that are under active investigation. (bvsalud.org)
  • This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)
  • It is highly specific and shows no cross-reaction with other related mitotic inhibitors. (neobiotechnologies.com)
  • Among them, cyclin-dependent kinases (CDK)s with their modulatory partners, cyclins, represent the major players acting with switch-like behavior to turn on cell growth, through the control of chromatin replication and condensation, gene transcription, assembly of the mitotic spindle, and proper cytodieresis. (mdpi.com)
  • In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. (neobiotechnologies.com)
  • Small‑molecule γ‑secretase inhibitors (AL101, MRK‑560, nirogacestat and others) and antibody‑based biologics targeting Notch ligands or receptors [ABT‑165, AMG 119, rovalpituzumab tesirine (Rova‑T) and others] have been developed as investigational drugs. (spandidos-publications.com)
  • MEN 4 is caused by an inactivating mutation of the CDKN1B gene, which codes for the cyclin dependent kinase inhibitor 1B protein, also known as p27 or p27KIP1. (merckmanuals.com)
  • and includes blood testing for hormone excess and genetic testing to identify the causative mutation in the CDKN1B gene. (merckmanuals.com)
  • Somatic mutation and germline sequence abnormalities in CDKN1B, encoding p27Kip1, in sporadic parathyroid adenomas. (cdc.gov)
  • Mutations in the CDKN1B gene have also been found to cause tumors in single endocrine glands, such as the pituitary gland or the parathyroid glands. (medlineplus.gov)
  • Somatic CDKN1B gene mutations have been identified in sporadic (nonfamilial) pituitary adenomas and in sporadic parathyroid adenomas, which are noncancerous tumors of the parathyroid glands. (medlineplus.gov)
  • The expression of both proteins was variable among different solid tumors including UC. (springeropen.com)
  • Precision Revisited: Targeting Microcephaly Kinases in Brain Tumors. (cancerindex.org)
  • Regulation can occur through modification of the p27 protein's structure, its interaction with other proteins, or its localization within the cell. (medlineplus.gov)
  • Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. (neobiotechnologies.com)
  • These events resulted in upregulation of the Cdk4/6 inhibitor p15 INK4B and repression of the Cdk-activating phosphatase Cdc25A. (shu.edu)
  • P15 INK4 is a cyclin dependent kinase inhibitor that blocks the activity of Cyclind-CDK4,6, inhibiting it from hypophosphorylating Rb, thereby, rendering the cell cycle unresponsive to external proliferation signals. (shu.edu)
  • Some mutations impair the protein's ability to interact with regulatory proteins, while others lead to the production of an unstable version of p27 that is quickly broken down. (medlineplus.gov)
  • The basic unit of the p53 protein consists of three major functional domains such as an N-terminal transactivation domain (TAD), a core DNA-binding domain (DBD)-the main target for mutations, and a C-terminal regulatory domain (CTD) ( Figure 1 A) [ 2 ] . (encyclopedia.pub)
  • Survivin is an apoptosis inhibitor that is associated with tumor proliferation and invasion. (springeropen.com)
  • P27 is a cyclin-dependent kinase inhibitor that negatively regulates cell proliferation. (springeropen.com)
  • The prolyl oligopeptidase inhibitor SUAM-14746 attenuates the proliferation of human breast cancer cell lines in vitro. (cancerindex.org)
  • The effects of POP inhibition and knockdown on the proliferation of cultured human estrogen receptor-positive (ER+) MCF7 and T47D, and ER-negative (ER-) MDA-MB-231 breast cancer cell lines and the MCF12A non-tumorigenic epithelial cell line were tested by analyzing their influence on cell proliferation (WST-1 assay), cell viability (trypan blue exclusion assay), and cell cycle arrest (cell cycle analysis, cell cycle regulator proteins expression). (cancerindex.org)
  • BCRC-3 functions as a tumor inhibitor to suppress BC cell proliferation through miR-182-5p/p27 axis, which would be a novel target for BC therapy. (biomedcentral.com)
  • These holoenzymes exist in distinct configurations or stable steady states, defined by various phosphatases and kinases that render the on/off switch rapid. (mdpi.com)
  • Furthermore, CDKN1B function requires both protein processing and RNA metabolism. (bvsalud.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • It acts as a tumor suppressor and when the protein is lost, cells can undergo unscheduled replication, which may eventually lead to cancer. (merckmanuals.com)
  • However, changes in regulation that reduce the amount or function of the p27 protein in the nucleus are found in many types of cancer. (medlineplus.gov)
  • In many experiments, CDKN1B level is associated with cancer risk, Nevertheless, no pan-cancer analysis has been conducted on CDKN1B in human cancers. (bvsalud.org)
  • METHODS: With the help of bioinformatics, a pan-cancer analysis was conducted on the expression levels of CDKN1B in cancer tissues and adjacent tissues from the TCGA, CPTAC and GEO databases. (bvsalud.org)
  • The CDKN1B gene encodes the p27Kip1 protein, which can block the production cyclin-dependent kinase (CDK), which is obviously related to the function and survival of cancer cells and alters the prognosis of cancer patients. (bvsalud.org)
  • Additionally, the elevated expression of the CDKN1B gene and protein was validated in several cancer tissues from the patients. (bvsalud.org)
  • CONCLUSIONS: These results showed that the levels of CDKN1B were considerably different in a number of cancer tissues, offering a potential future target for cancer therapy. (bvsalud.org)
  • The protein is involved in cellular growth and development and has an important role in regulating the cell cycle. (merckmanuals.com)
  • As an alternative approach to such conventional inhibitors, the first-in-class small molecule sEH degraders were recently developed by our group that promote the degradation of sEH within cells with superior cellular efficacy compared to sEH inhibitors. (einsteinmed.edu)
  • A ) Simplified representation of the secondary structure showing domain organization of the human p53 protein (Uniprot #P04637). (encyclopedia.pub)
  • Although first-generation soluble epoxide hydrolase (sEH) inhibitor entered clinical trials more than 10 years ago for the treatment of insulin resistance and diabetes, there are currently still no drugs on market targeting this promising therapeutic target for diabetes. (einsteinmed.edu)
  • The p53 protein, to exert its function, binds in a sequence-specific manner to the DNA-binding sites by forming a tetramer, via four self-assembling p53 molecules, which are stabilized by protein-protein and base-stacking interactions [ 3 ] . (encyclopedia.pub)
  • Objective: The aim of this study was to evaluate the prognostic value of Butyrophilin-like protein 8 (BTNL8) expression in high-risk HPV (hrHPV) infection treated with photodynamic therapy. (bvsalud.org)
  • The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. (wikipedia.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • This protein is found in cells and tissues throughout the body. (medlineplus.gov)
  • The type IIcytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratinchains coexpressed during differentiation of simple and stratified epithelial tissues. (thebiotechdictionary.com)
  • studies suggest that certain endocrine cells may be particularly dependent on the p27 protein to control cell division. (medlineplus.gov)
  • Cells have a protein on their surface called PD-1 (in orange above). (shu.edu)
  • EVs are a natural carrier system that can transfer nucleic acids, proteins, and lipids between donor and recipient cells in an autocrine, paracrine, and endocrine manner. (imrpress.com)
  • Receptor-regulated SMADs (R-SMADs), SMAD1, 2, 3, 5, and 8, are the only SMADs directly phosphorylated and activated by the kinase domain of type I receptors. (shu.edu)
  • This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. (cancerindex.org)
  • The CDKN1B expression levels in tumor patients was further validated using immunohistochemistry (IHC) and quantitative real-time PCR. (bvsalud.org)