• ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • The CD45RA antibody recognizes a 220-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). (bdbiosciences.com)
  • The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. (cytekbio.com)
  • The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. (cytekbio.com)
  • The antibody is reported to be cross-reactive for Chimpanzee CD45RO. (cytekbio.com)
  • The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • 300 cells/mm3, normal in vitro T-cell proliferative responses (to mitogens and antigens), and normal specific antibody responses to non-live viral vaccine antigens. (immunodeficiencysearch.com)
  • IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
  • This anti-CD45 antibody is available for research use only. (nsjbio.com)
  • This antibody will bind to all CD45 isoforms. (nsjbio.com)
  • Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (nsjbio.com)
  • Due to variation in protocol and secondary antibody used, the anti-CD45 antibody may need to be titered for optimal performance. (nsjbio.com)
  • Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
  • Anti-CD45 antibody (with azide) can be stored at 2-8oC. (nsjbio.com)
  • CAR45 constructs were created by cloning anti-CD45 single-chain variable fragments (scFvs) from a selected antibody clone, BC8, which recognizes CD45, into second-generation CAR lentiviral vectors. (acir.org)
  • Two gRNAs were identified that resulted in a single amino acid substitution at the CD45 epitope, which resulted in a complete lack of CD45 binding by the BC8 antibody. (acir.org)
  • All 4 patients had evidence of specific antibody responses against potential tumor antigens. (biomedcentral.com)
  • CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
  • CD45 contains a Tn antigen in exon B. CD45 has 3 important exons (4,5,6), that are designated A,B,C. Isoforms of CD45 are labeled depending on the presence of these exons. (wikipedia.org)
  • However, active T cells express shorter isoforms of CD45 (CD45RO, CD45RA) that lack exon B. CLEC10A signalling induces IL-10 production in dendritic cells, in part through increasing intracellular Ca2+ concentration. (wikipedia.org)
  • CD45R and CD45RO are two of multiple PTPRC isoforms generated by alternative splicing. (ximbio.com)
  • With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
  • Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
  • Conventional monoclonal antibodies(mAbs) raised against CD45 have reactivity against all isoforms, while restricted mAB only reacts to specific isotypes (CD45RA, CD45RB, CD45RO). (biomabinc.com)
  • Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
  • CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. (adipogen.com)
  • Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) Brilliant Violet 605™ (top) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (bottom). (biolegend.com)
  • The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens. (bdbiosciences.com)
  • Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB. (cytekbio.com)
  • The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). (abeomics.com)
  • Antibodies to common leukocyte antigen p220 influence human T cell proliferation by modifying IL 2 receptor expression. (bdbiosciences.com)
  • CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). (cytekbio.com)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (exbio.cz)
  • CD45, also known as leukocyte common antigen (LCA), protein tyrosine phosphatase, receptor type I (PTPR) is a type I transmembrane protein encoded by PTPR gene specifically expresses in hematopoietic cells. (biomabinc.com)
  • 1987). Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. (biomabinc.com)
  • gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells. (bdbiosciences.com)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (exbio.cz)
  • CD45RO plays an essential role in lymphocyte activation. (adipogen.com)
  • Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)
  • The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions. (bdbiosciences.com)
  • CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
  • CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)
  • PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. (ximbio.com)
  • PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. (ximbio.com)
  • The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. (biolegend.com)
  • CD45RO is present on 50% of T cells, most granulocytes and monocytes and most mature T cell tumours. (ximbio.com)
  • The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. (adipogen.com)
  • It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. (biolegend.com)
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (neobiotechnologies.com)
  • Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. (bdbiosciences.com)
  • CD45 isoform expression can change in response to cytokines. (biolegend.com)
  • Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
  • Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
  • CD45RO doesn't contain A, B or C and is a marker of activated T-cells that can be used to classify and diagnose and classify lymphomas. (nsjbio.com)
  • Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
  • Function of T helper cells: Antigen presenting cells ( APCs ) present antigen on their Class II MHC molecules ( MHC2 ). (wikidoc.org)
  • Class II MHC proteins are generally only found on the surface of professional antigen-presenting cells (APCs). (wikidoc.org)
  • Some APCs also bind native (or unprocessed) antigens to their surface, such as follicular dendritic cells , but unprocessed antigens do not interact with T cells and are not involved in their activation. (wikidoc.org)
  • During an immune response, professional antigen-presenting cells (APCs) endocytose (absorb) foreign material (typically bacteria or viruses ), which undergoes processing , then travel from the infection site to the lymph nodes . (wikidoc.org)
  • Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)
  • CD45 has also been reported to bind galectin-1 and CD22. (biolegend.com)
  • The antigens that bind to MHC proteins are always short peptides , 8-10 amino acids long for MHC Class I, and up to 25 or so for MHC Class II. (wikidoc.org)
  • CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. (biolegend.com)
  • When NALM6 cells with CD45 alanine mutants were cocultured with CD45KO CAR primary human T cells, four mutation constructs reduced or removed BC8-derived CAR T cell activation. (acir.org)
  • Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells. (wikidoc.org)
  • Following T cell development , matured, naïve (meaning they have never been exposed to the antigen to which they can respond) T cells leave the thymus and begin to spread throughout the body, including the lymph nodes . (wikidoc.org)
  • Professional antigen presenting cells are primarily dendritic cells , macrophages and B cells , although dendritic cells are the only cell group that expresses MHC Class II constitutively (at all times). (wikidoc.org)
  • Once at the lymph nodes, the APC begins to present antigen peptides that are bound to Class II MHC, allowing CD4 + T cells that express specific TcR's against the peptide/MHC complex to activate. (wikidoc.org)
  • Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
  • CAR T cell therapy for hematological cancers can be highly effective, but is currently limited to antigens that are only expressed by tumor cells or by lineages that are non-essential. (acir.org)
  • Targeting antigens expressed by all tumor cells is often associated with on-target/off-tumor toxicities to normal hematopoietic cells. (acir.org)
  • aimed to target CD45, as it is expressed by most hematological cancer cells. (acir.org)
  • However, CD45 is expressed on all hematopoietic stem cells and their progeny, including the CAR T cells themselves, and is required for their function. (acir.org)
  • Therefore, even though CD45 CAR T cells (CAR45) would target leukemias and lymphomas, they would be limited by fratricide, and would result in pancytopenia due to on-target/off-tumor toxicity. (acir.org)
  • Initially the researchers expressed myc-tagged CD45RO constructs that sequentially truncated the ectodomain from the N-terminus in CD45- NALM6 cells. (acir.org)
  • Alanine was substituted for each candidate amino acid in CD45, and mutant CD45 constructs were expressed in NALM6 cells. (acir.org)
  • Transduction of CAR45 into a mixed population of T cells with or without epitope editing of CD45, resulted in the elimination of non-edited cells and enrichment of edited cells. (acir.org)
  • The CAR45 cells were able to kill CD45 WT -expressing cells, while they could not kill CD45 BE (base-edited) cells. (acir.org)
  • However, this CAR-T construct would lead to on-target/off-tumor toxicities against healthy hematopoietic cells, which express CD45. (acir.org)
  • Finally, the researchers hypothesized that epitope editing could also protect cells from bispecific T cell engagers (BTE) targeting CD45. (acir.org)
  • Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. (adipogen.com)
  • Expression of neoantigens alone is insufficient without cross-presentation by antigen presenting cells in the appropriate cytokine milieu. (biomedcentral.com)
  • In addition to mutated proteins, NSCLC can over-express a number of non-mutated proteins that can induce both humoral and cytotoxic T cell responses [ 10 ], and also have the potential to serve as tumor rejection antigens [ 11 ]. (biomedcentral.com)
  • Superimposing the amino acids relevant for binding to the BC8 CAR onto the crystal structure of the CD45 extracellular domain revealed a conformational epitope. (acir.org)
  • These tumor-associated antigens (Neu5Acα2,6-Tn, and NeuGcα2,6-Tn) are also bound. (wikipedia.org)
  • Study therapy included two cycles of docetaxel 75 mg/m 2 on days 1 and 29 to treat the tumor, release hidden antigens and produce lymphopenia. (biomedcentral.com)
  • To overcome fratricide, the researchers aimed to identify a non-synonymous mutation at a CD45-relevant epitope using CRISPR base editing, so that CAR45 would not recognize it, while simultaneously maintaining the expression and function of CD45. (acir.org)
  • To introduce the mutation in the epitope on CD45 targeted by CAR45, CRISPR base editing was used to install base pair changes at the targeted loci. (acir.org)
  • None of the mutations affected CD45 expression. (acir.org)
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
  • The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. (thermofisher.com)
  • CD45RO is a 180 kD single chain membrane glycoprotein. (biolegend.com)
  • This includes the Tn antigen (GalNAc O-bound to serine or threonine) which is prominently expressed on carcinomas, where it can also be sialylated. (wikipedia.org)