Cd45r antigens cd45. Medical search. Frequent questions

Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA and CD45RB. (cytekbio.com)
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of CD3 Monoclonal Antibody, Super Bright 600 (right). (thermofisher.com)
CD45R, also known as B220, is an isoform of CD45. (biolegend.com)
CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL- Fas lpr and MRL- Fas gld mice. (biolegend.com)

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). (rndsystems.com)
PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. (ximbio.com)
PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. (ximbio.com)
CD45R and CD45RO are two of multiple PTPRC isoforms generated by alternative splicing. (ximbio.com)
CD45, also referred to as CD45R and PTPRC (Protein tyrosine phosphatase receptor type C), has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (nsjbio.com)
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)

Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15). (rndsystems.com)
CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). (rndsystems.com)
The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. (bdbiosciences.com)
The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. (bdbiosciences.com)
CD45 contains a Tn antigen in exon B. CD45 has 3 important exons (4,5,6), that are designated A,B,C. Isoforms of CD45 are labeled depending on the presence of these exons. (wikipedia.org)
However, active T cells express shorter isoforms of CD45 (CD45RO, CD45RA) that lack exon B. CLEC10A signalling induces IL-10 production in dendritic cells, in part through increasing intracellular Ca2+ concentration. (wikipedia.org)
With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
Multiple isoforms of CD45 are distributed throughout the immune system and arise due to alternative splicing of exons located in the N-terminus. (nsjbio.com)
This antibody will bind to all CD45 isoforms. (nsjbio.com)
Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. (abeomics.com)
The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. (abeomics.com)
CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. (adipogen.com)

The 16A monoclonal antibody specifically recognizes an exon B-dependent epitope of CD45 lycoprotein, which is found at high density on peripheral B cells, T cytotoxic/suppressor cells, a subset of T helper cells, and most thymocytes, and at low density on macrophages and dendritic cells. (bdbiosciences.com)
Bottomly K, Luqman M, Greenbaum L. A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines. (bdbiosciences.com)
The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of ≥ 220 kDa. (cytekbio.com)
IHC testing of FFPE human tonsil with anti-CD45 antibody (clone SPM570). (nsjbio.com)
This anti-CD45 antibody is available for research use only. (nsjbio.com)
Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (nsjbio.com)
Due to variation in protocol and secondary antibody used, the anti-CD45 antibody may need to be titered for optimal performance. (nsjbio.com)
Neoplastic cells from a T cell lymphoma were used as the immunogen for the anti-CD45 antibody. (nsjbio.com)
Anti-CD45 antibody (with azide) can be stored at 2-8oC. (nsjbio.com)
The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
en Ksp-Cadherin (MRQ-33) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. (roche.com)
In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. (roche.com)
Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. (roche.com)

CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). (rndsystems.com)
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. (bdbiosciences.com)
This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (origene.com)
It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. (origene.com)
Along with other members of the PTP family, it regulates a number of cellular processes including cell differentiation, growth and mitotic cycle, and is an essential regulator of B- and T-cell antigen receptor-mediated activation. (nsjbio.com)
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (neobiotechnologies.com)

CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). (cytekbio.com)

CLEC10A can for example bind CD45RB or CD45R, which is shorthand for CD45RABC. (wikipedia.org)

Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. (bdbiosciences.com)
Accurate Chemical supplies mouse host antibodies and antigens to Life Science research facilities worldwide. (accuratechemical.com)
Magnetically labeled antibodies to RAD001 , CD45R, and CD49b antigens, ordered from Miltenyi Biotec, had been used to isolate populations that were enriched for macrophages, B lymphocytes, and NK cells, respectively, whereas magnetically labeled anti CD4 and anti CD8a antibodies had been used to fractionate out the two subsets of T lymphocytes. (hifsignaling.com)
The purity of each fraction was established by flow cytometry following labeling of the positively selected subpopulation with FITC conjugated antibodies to the antigen utilized for assortment. (hifsignaling.com)

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. (biolegend.com)

BD Tritest™ CD3 fluorescein isothiocyanate (FITC)/CD4 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP) is a three-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T lymphocytes (CD3 + ) and helper/inducer (CD3 + CD4 + ) T-lymphocytes subsets in erythrocyte-lysed whole blood. (bdbiosciences.com)

The CD45 leukocyte infiltrate in Colon 38 tumors was proven by FACS assessment to comprise CD4 T cells, CD45R B lymphocytes, immature macrophages/monocytes, mature macrophages, and CD49b NK cells. (hifsignaling.com)
CD19 CD45R B lymphocytes, CD49b NK cells, and Elvitegravir CD8a and CD3 CD4 subsets all diminished in variety for the duration of the very first 3 days, then improved to pretreatment ranges right after 7 days, and then stabilized. (pimsignaling.com)

Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. (adipogen.com)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)

The quantity of CD45 leukocytes per gram of tumor elevated three fold in the course of the initial 24 hrs right after remedy when the tumors dimension was decreasing. (hifsignaling.com)
The variety of CD45 leukocytes per gram of tumor enhanced three fold for the duration of the first 24 hours after therapy when the tumors dimension was reducing. (pimsignaling.com)

In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). (rndsystems.com)
Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories: T2 protocol. (bdbiosciences.com)
Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)

CD45 (human):Fc (mouse) (rec. (adipogen.com)

Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). (rndsystems.com)

CD45R functions as a phosphor-tyrosine phosphatase. (abeomics.com)

LewisY blood antigen or simply BG8, is a blood group antigen that has been identified in studies as a potential marker for differentiating pulmonary adenocarcinoma (PACA) from epithelioid mesothelioma (EM). (neuromics.com)

CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. (biolegend.com)
In a second experiment, CD11b and CD8 and/or Compact disc 4 cells have been isolated from the a single pool of ten spleens, and CD49b and CD45R and/or CD4 were isolated from a second pool of ten spleens. (hifsignaling.com)
In a second experiment, CD11b and CD8 and/or Cd 4 cells had been isolated from the one particular pool of ten spleens, and CD49b and CD45R and/or CD4 had been isolated from a second pool of 10 spleens. (her2signaling.com)
Moreover, the shorter complementarity-determining region 3 (CDR3) amino acid (AA) length from IgMhiCD300a+ B cells together with the predicted antigen experience repertoire indicates that this B cell subset has a memory phenotype. (bvsalud.org)
We isolated fecal content and small intestinal leukocytes from murine, Roux-en-Y and end-to-end anastomoses, to identify microbiome changes and subsequent alterations in the regulatory and pro-inflammatory immune cells 3 days post-operative. (biomedcentral.com)
CD45 leukocytes then dropped from DPP-4 10 to a nadir of cells per gram tumor excess weight on day 3, ahead of growing and stabilizing at 10 cells following 7 days. (pimsignaling.com)

Detects mouse CD45. (rndsystems.com)

The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. (roche.com)

Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. (bdbiosciences.com)

CD45R is found on B lymphocyte and T lymphocyte subsets. (ximbio.com)

CD45 Protein, Human (HEK 293, His) is a type 1 transmembrane protein tyrosine phosphatase (PTPase) expressed by all the haematopoietic stem cells (HSCs) except that of erythrocytes and platelets. (medchemexpress.com)
CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. (exbio.cz)
CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. (exbio.cz)
Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "CD45R, also named MB1, is the isoform of CD45, the protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. (roche.com)
CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. (cytekbio.com)
CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. (stemcell.com)

CD45 is a transmembrane glycoprotein, expressed on almost all haematopoietic cells except for mature erythrocytes. (medchemexpress.com)
The antibody MEM-56 reacts with an extracellular epitope of CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). (exbio.cz)
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. (biolegend.com)

The 16A monoclonal antibody specifically recognizes an exon B-dependent epitope of CD45 lycoprotein, which is found at high density on peripheral B cells, T cytotoxic/suppressor cells, a subset of T helper cells, and most thymocytes, and at low density on macrophages and dendritic cells. (bdbiosciences.com)
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. (thermofisher.com)
A splicing isoform of CD45 antigen that includes exon B. (nih.gov)

The following product was used in this experiment: CD45R (B220) Monoclonal Antibody (RA3-6B2), NovaFluor™ Blue 510, eBioscience™ from Thermo Fisher Scientific, catalog # M011T02B01, RRID AB_2910797. (thermofisher.com)
BALB/c mouse splenocytes were unstained (left) or stained with 1 µg of CD45R Monoclonal Antibody, NovaFluor Blue 510 (right). (thermofisher.com)
19. Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. (nih.gov)
ProductData": { "ID": "RTD000729", "ProductType": "Others", "BrandName": "CD45R (MB1) Mouse Monoclonal Antibody", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "CD45R (MB1) Mouse Monoclonal Antibody (this antibody) may be used as the primary\nantibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue\nsections. (roche.com)

CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. (thermofisher.com)
Analysis by electron microscopy and use in epitope mapping of anti-CD45R monoclonal antibodies. (nih.gov)

Rat anti Mouse CD45R antibody, clone RA3-6B2 ( MCA1258 ) used for the enumeration of B cells in the intestinal mucosa by immunofluorescence. (bio-rad-antibodies.com)
C57BL/6 splenocytes were stained with anti-mouse CD3 PE and anti-mouse/human CD45R/B220 (clone RA3-6B2) Spark Violet™ 423 (left) or rat IgG2a, ĸ Spark Violet™ 423 isotype control (right). (biolegend.com)

3. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. (nih.gov)
CD45R immunohistochemical reactivity is reportedly seen in most B-lymphocytes such as follicle center cells, mantle cells, some medullary thymocytes, post-thymic naïve T-lymphocytes, and B-cell lymphomas. (roche.com)

13. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. (nih.gov)
Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)

CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
Studies of monoclonal antibodies identifying two novel bovine lymphocyte antigen differentiation clusters: workshop clusters (WC) 6 and 7. (ox.ac.uk)
During lymphocyte maturation, immune cells will express complex variations of CD antigens on its cell surface, some of which are lost, while others are acquired at various stages either by interactions with antigen presenting cells (APC) or with other cells of the immune system. (stratech.co.uk)

expression of CD45 is lost during differentiation of these cell lineages. (stemcell.com)
In flow cytometric immunophenotyping, monoclonal antibodies targeting CD antigens are extensively used for the identification and characterization of leukocyte populations, subpopulations and differentiation stages. (stratech.co.uk)

Because CD antigens are expressed at varying degrees and cell types, multiparameter flow cytometric analysis utilizing two or more combinations of fluorescently labeled CD antibodies enables clear resolution of particular cell type or subtype. (stratech.co.uk)
Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). (goodtimebio.com)

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (cusabio.com)

6. Isoform-specific associations of CD45 with accessory molecules in human T lymphocytes. (nih.gov)

A low molecular weight isoform of CD45 antigen. (nih.gov)

The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes. (exbio.cz)
CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL- Fas lpr and MRL- Fas gld mice. (biolegend.com)

With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. (cytekbio.com)
CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. (biolegend.com)

Conventional CD45, CD45R, 180 kDa (UCHL1) and 220/205/190 kDa. (nih.gov)

Differential quantitative expression of T-200 common leukocyte antigen during normal hemopoiesis. (nih.gov)
Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be\nassociated with a particular antigen. (roche.com)

If the surface molecule is well characterized (recognized by two or more mAbs), then they are assigned an arbitrary value, such as CD4, CD8 or CD45. (stratech.co.uk)

Demonstration of lymphoid antigens in decalcified bone marrow trephines. (roche.com)

This antigen is also expressed on the surface of cells of myeloid lineage. (m-entrepreneurship.com)
For example, CD3 is a T cell co-receptor exclusively expressed on the surface of all mature T cells, while CD4 and CD8 antigens are expressed on the surface of T helper cells and cytotoxic T cells, respectively. (stratech.co.uk)
The presence or absence of a specific antigen from the surface of the particular cell population is denoted with "+" or "-" respectively. (stratech.co.uk)

ESC-derived, cytokine-induced HSCs (c-kit + /CD45 + ) were isolated by flow cytometry and injected either intra bone marrow or intravenously into lethally irradiated MHC-mismatched recipient mice. (rupress.org)

The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains, one of which is enzymatically active, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. (stemcell.com)

18. Glycosylation of CD45: carbohydrate composition and its role in acquisition of CD45R0 and CD45RB T cell maturation-related antigen specificities during biosynthesis. (nih.gov)

11. Leucocyte common antigen expression on T cells in normal and inflamed human gut. (nih.gov)
Five-year disease-free survival for patients with a high transcriptional expression of CD45 (n = 107) was 62.4% and for patients with a low expression (n = 53) it was 36.2% (P = 0.003). (cusabio.com)
Patients with a high expression of CD45 had a better local recurrence-free survival and disease-specific survival. (cusabio.com)
Variant CD45R expression with autosomal dominant inheritance affects both helper/inducer (CD4+) and suppressor/cytotoxic (CD8+) T cell populations. (roche.com)
In addition, CD antigen expression can be influenced by certain disease conditions, and as such are often used as diagnostic or prognostic factors for the identification of leukemia's and lymphomas. (stratech.co.uk)

For example, a 'CD45+/CD20-' cell is one that expresses CD45, but not CD20. (stratech.co.uk)

Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "CD45R (MB1) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the CD45R protein in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. (roche.com)
Figure B. Alexa488® conjugated rat anti mouse CD3 ( MCA2690A488 ) and Alexa647® conjugated rat anti mouse CD45 ( MCA1031A647 ). (bio-rad-antibodies.com)
Rat anti Mouse CD45 antibody, clone YW62.3 ( MCA1031GA ) used for the detection of microglia in mouse brain by immunohistochemistry on cryostat sections. (bio-rad-antibodies.com)
Human IgG antibody Laboratories manufactures the rat anti-mouse cd45 igg2b antibody (pan-leukocyte marker reagents distributed by Genprice. (bionotatki.com)
The Rat Anti-Mouse Cd45 Igg2B Antibody (Pan-Leukocyte Marker reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bionotatki.com)

14. Different CD45 and CD45R epitopes involved in T cell proliferation and NK cytotoxicity. (nih.gov)

10. Evidence for an association of CD45 with 32,000-33,000 MW phosphoproteins on murine T and B lymphocytes. (nih.gov)

Anatomy34

Organisms11

Diseases5

Chemicals and Drugs150

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes34

Disciplines and Occupations1

Information Science3

Health Care1

B2204

Ptprc6

Isoforms12

Antibody13

Receptor6

Leukocyte common1

CD45RB1

Antibodies4

Clone1

FITC1

CD49b NK2

Hematopoietic1

Tonsil1

Tumors2

Differentiation3

Human1

Regulates1

Functions1

Marker1

Cells6

Mouse1

Activation1

Previously1

Found1

Protein Tyrosine Ph6

Glycoprotein3

Exon3

Monoclonal Antibody5

Epitope2

Clone RA3-6B22

Reactivity2

Isoform expression2

Lymphocyte3

DIFFERENTIATION2

Antibodies2

Tyrosine-protein phosphatase1

Molecules1

Molecular weight1

Expressed on a subset2

Signal transduction2

UCHL11

Differential2

Molecule1

Lymphoid1

Surface3

HSCs1

Intracellular1

Carbohydrate1

Expression5

Expresses1

Mouse5

Cell1

Murine1