• Mutations in the CD40 ligand (CD40L) are responsible for human hyper immunoglobulin M (IgM) syndrome. (nki.nl)
  • The absence of the interaction between CD40L, expressed by T lymphocytes, and the CD40 receptor present on the surface of B cells is responsible for the inability of B cells to carry out the isotype switch from IgM to the other Ig classes. (nki.nl)
  • For the quantification of CD40 ligand (CD40L) in Culture Supernatant, Serum, Plasma (EDTA), Plasma (Heparin). (biosensis.com)
  • Scientific Background CD40L is the ligand for CD40, a member of the tumour necrosis factor (TNF) receptor superfamily. (biosensis.com)
  • Platelets express CD40L on activation, which induces proinflammatory changes in endothelial cells via endothelial CD40. (biu.ac.il)
  • The role of CD40L in the immune response involves binding to its receptor on B cells, CD40, to induce B-cell proliferation, generate memory B cells, block B-cell apoptosis, and mediate antibody class switching. (biu.ac.il)
  • To test this hypothesis, both an Ag‐specific mouse B cell clone CH12.LX, and freshly isolated resting splenic B cells were cultured with either soluble or membrane‐bound forms of the T cell ligand for CD40 (CD40L), in the presence or absence of additional signals provided by Ag or anti‐IgM, interleukin‐4, and class II‐specific monoclonal antibody (mAb). (uthscsa.edu)
  • Similar to CpG ODN, CD40 ligand (CD40L) alone induced IL-6, TNF-α, and IL-10. (aai.org)
  • In this study, we investigated the involvement of CD40 ligand (CD40L)-mediated interactions in these processes by analyzing the memory CTL response of CD40L-deficient mice following infection with lymphocytic choriomeningitis virus (LCMV). (ox.ac.uk)
  • We expressed both CD40L and OX40L on B-CLL cells by exploiting the phenomenon of molecular transfer from fibroblasts overexpressing these ligands. (houstonmethodist.org)
  • The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis. (biolegend.com)
  • Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis. (biolegend.com)
  • CD40 ligand (CD40L) is mainly expressed on activated platelets and CD4+T cells, and it can be cleaved from the cell surface, releasing a soluble CD40L (sCD40L). (researchgate.net)
  • RÉSUMÉ L'objectif de l'étude était d'évaler l'importance clinique du ligand de CD40 soluble (sCD40L) chez des patients atteints d'un carcinome hépatocellulaire (CHC) associé au virus de l'hépatite C (VHC). (who.int)
  • Les concentrations sériques de sCD40L circulant et d'interleukine 10 circulante ont été analysées à l'aide de la méthode immuno-enzymatique chez 30 patients positifs pour le VHC avec un CHC, chez 30 patients patients positifs pour le VHC avec une cirrhose du foie, et chez 30 volontaires d'âge correspondant en bonne santé avec des anticorps anti-VHC négatifs servant de groupe témoin. (who.int)
  • This study evaluated the effects of exogenous soluble CD40 ligand (sCD40L) on RNA-transfected DC preparations and their subsequent ability to generate antimelanoma cytolytic T cells. (duke.edu)
  • Although elevated serum levels of soluble CD40 ligand (sCD40L) were reported in patients with cancer , the importance of high sCD40L levels in clinical oncology remains unknown. (bvsalud.org)
  • Soluble CD40 ligand (sCD40L) is involved in the pathogenesis of risk factor-related vascular damage. (comu.edu.tr)
  • Soluble CD40 ligand (sCD40L) derived from platelets mediates atherothrombosis, leading to proinflammatory and proatherosclerotic responses. (regionh.dk)
  • We aimed to clarify whether soluble CD40 ligand (sCD40L) activated B cells may be loaded with HBcAg18-27 peptide and served as antigen-producing cells (APCs) to induce HBV-specific cytolytic T lymphocytes (CTLs). (biomedcentral.com)
  • Signals delivered by the CD40 ligand, CD154, have crucial roles in immune responses in mammals, being required for development of germinal centres, maturation of T-dependent antibody responses, and generation of B-cell memory. (lincoln.ac.uk)
  • CD154) is a transmembrane protein expressed on the surface of activated T cells, particularly on CD4 T cells, which stimulates CD40-dependent activation of antigen-presenting cells (APCs), resulting in enhancement of T cell and antibody responses. (biospace.com)
  • The ligand of CD40 is CD154 (CD40 ligand). (biolegend.com)
  • CD154 has been kown a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. (biolegend.com)
  • CD154 is an accessory molecule expressed predominantly on activated CD4 + lymphocytes that bind CD40. (biolegend.com)
  • Most cases are X-linked and caused by mutations in a gene on the X chromosome that encodes a protein (CD154, or CD40 ligand) on the surfaces of activated helper T cells. (msdmanuals.com)
  • Bishop, GA, Warren, WD & Berton, MT 1995, ' Signaling via major histocompatibility complex class II molecules and antigen receptors enhances the B cell response to gp39/CD40 ligand ', European Journal of Immunology , vol. 25, no. 5, pp. 1230-1238. (uthscsa.edu)
  • The present study was designed to test the hypothesis that signals delivered via the B cell antigen (Ag) receptor (membrane immunoglobulin, mIg) and major histocompatibility complex (MHC) class II molecules enhance B cell responsiveness to CD40‐mediated signals, providing specificity to the Ag‐nonspecific, MHC‐unrestricted CD40 signal. (uthscsa.edu)
  • To investigate association of apoptosis in the pathogenesis of EBV-associated HLH, the serum EBV loads, and serum concentrations of soluble tumor necrosis factor receptor 1 (sTNF-R1), soluble Fas ligand, and cytochrome c were examined in 15 patien. (researchgate.net)
  • Activated T cells induce proliferation and differentiation of resting B cells in vitro through their CD40 molecules and lymphokine receptors. (uthscsa.edu)
  • However, despite constitutive B cell expression of CD40 and lymphokine receptors, widespread nonspecific polyclonal B cell activation by activated T cells is seldom observed in vivo. (uthscsa.edu)
  • The ability of class II MHC‐mediated signals to enhance Ag‐specific B cell responsiveness to CD40‐mediated signaling may selectively promote the activation of B cell clones capable of cognate interactions with helper T cells. (uthscsa.edu)
  • Childhood B cell precursor acute lymphoblastic leukemia (BCP-ALL) cells, collected from bone marrow (BM) at diagnosis, were cultured, after thawing, on allogeneic human bone marrow stroma (HBMS) for 48 h in the presence of a soluble trimeric CD40 ligand (stCD40L) molecule. (unimib.it)
  • FLT-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. (topsan.org)
  • Alone, flt-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. (topsan.org)
  • flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes. (topsan.org)
  • The OX40-OX40 ligand pathway is involved in the subsequent expansion of memory antigen-specific T cells. (houstonmethodist.org)
  • CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. (biolegend.com)
  • 1 Alone, or in combination with TLR ligands, clone HIB14 stimulates B cells to produce IL-10 and differentiates it into regulatory B10 (IL-10 producing B cells). (biolegend.com)
  • Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. (bdbiosciences.com)
  • CD40 ligand trimer enhances the response of CD8 + T cells to Mycobacterium tuberculosis. (southernbiotech.com)
  • In the presence of cytokines, normal CD40 ligand interacts with B cells and thus signals them to switch from producing IgM to producing IgA, IgG, or IgE. (msdmanuals.com)
  • In X-linked hyper-IgM syndrome, T cells lack functional CD40 ligand and cannot signal B cells to switch. (msdmanuals.com)
  • Lymphoid tissue is very small because deficient CD 40 ligand signaling does not activate B cells. (msdmanuals.com)
  • CD40 expression on platelets was significantly higher in patients who had an ischemic stroke compared with patients with asymptomatic carotid stenoses. (biu.ac.il)
  • Deletions in the ligand for CD40 in X-linked immunoglobulin deficiency with normal or elevated IgM (HIGMX-1). (nih.gov)
  • CD40 ligand-mediated interactions are involved in the generation of memory CD8(+) cytotoxic T lymphocytes (CTL) but are not required for the maintenance of CTL memory following virus infection. (ox.ac.uk)
  • Human peripheral blood lymphocytes stained with CD40 (clone HB14) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram). (biolegend.com)
  • lymphocytes that bind CD40. (biolegend.com)
  • Activation of DC surface CD40 influences DC cytokine production, particularly that of interleukin (IL)-12, which favors a Th1 (cytotoxic) helper T cell response. (duke.edu)
  • CD40 ligand is an accessory signal for T-cell activation and can overcome T-cell anergy. (houstonmethodist.org)
  • Scholars@Duke publication: CD40 ligand is essential for generation of specific cytotoxic T cell responses in RNA-pulsed dendritic cell immunotherapy. (duke.edu)
  • CD40 is a 48-kDa phosphorylated transmembrane glycoprotein with important role in adaptive immunity and inflammation. (biu.ac.il)
  • Under these culture conditions we noticed upregulation or de novo expression of costimulatory molecules CD40, CD80 (B7-1) and CD86 (B7-2) in 22/22, 15/23 and 21/23 cases, respectively. (unimib.it)
  • Role of Fas ligand expression in promoting escape from immune rejection in a spontaneous tumor model. (southernbiotech.com)
  • Additionally, CD40 is important for T cell-B cell interactions. (biolegend.com)
  • The multiple roles for sCD40 ligand implicate it as a mediator of adverse events among patients with cardiovascular disease. (biu.ac.il)
  • CD40 is a 48 kD type I glycoprotein also known as BP50. (biolegend.com)
  • Reduced Relapse-Free Survival in Colorectal Cancer Patients with Elevated Soluble CD40 Ligand Levels Improved by Vitamin D Supplementation. (bvsalud.org)
  • In autosomal recessive hyper-IgM syndrome with CD40 mutation, manifestations are similar to those of the X-linked form. (msdmanuals.com)
  • It's really novel, a new soluble CD40 ligand. (newshealth.biz)
  • The role of soluble CD40 ligand (sCD40L) as a potential initiator of inflammation after stenting has not been described. (biu.ac.il)
  • On this basis engagement of CD40 on DC to induce anti-tumour immune responses is a prolific area of research and both recombinant soluble CD40 ligand and CD40 agonist antibodies have entered clinical trials. (scienceexhibitions.org)
  • The relationship of platelet number to soluble CD40 Ligand, Interleukin-6 and C-reactive protein blood levels was studied. (scirp.org)
  • Expression of CD40 Ligand on T Cells and Soluble CD40 Ligand in Children With Kawasaki Disease: A Single-Center Preliminary Study From North India. (nih.gov)
  • Collaborating with a team at Biogen Inc., we determined the crystal structure of CD40-ligand 2 , developed a humanized version of our antibody (hu5c8, ruplizumab, or Antova®) and tested it in human trials for preventing organ transplant rejection and autoimmunity. (tonixpharma.com)
  • Long-term human B cell lines dependent on interleukin-4 and antibody to CD40. (wikipedia.org)
  • 17. TLR9 Ligand (CpG Oligodeoxynucleotide) Induces CLL B-Cells to Differentiate into CD20(+) Antibody-Secreting Cells. (nih.gov)
  • Anti-human CD3 monoclonal antibody OKT3, combined with 5C3 antibody against CD40, was used as stimulant. (hindawi.com)
  • Furthermore, the ability of mCD40L to also directly induce cell death in CD40-expressing carcinomas, subsequently releasing tumour-specific antigens into the tumour microenvironment highlights the potential for mCD40L as a multi-faceted anti-cancer immunotherapeutic. (whiterose.ac.uk)
  • Tonix's President and Chief Executive Officer, Seth Lederman, M.D. said, "Nearly 30 years ago my laboratory at Columbia University generated the first anti-CD40-ligand mAb (5c8), discovered and characterized human CD40-ligand and elucidated the molecular basis of T cell helper function 1 . (tonixpharma.com)
  • Our studies and those of others generated a substantial body of evidence in humans and animals that indicates anti-CD40-ligand mAbs have the potential to be an important therapeutic option for preventing or treating transplant organ rejection and for treating autoimmune disorders. (tonixpharma.com)
  • Second generation anti-CD40-ligand mAbs had dramatically reduced binding to FcγRII, but had other issues, including decreased efficacy 3-5 . (tonixpharma.com)
  • TNX-1500 is a third generation anti-CD40-ligand mAb that has been designed by protein engineering to decrease FcγRII binding and the potential for thrombosis, while retaining efficacy. (tonixpharma.com)
  • We will continue to apply our HERA-ligand technology to address other TNF superfamily receptors that play a critical role in the antitumor immune response and evaluate the synergistic potential of these development candidates in combination with traditional cancer therapies as well as other immuno-oncology therapeutics. (drugdiscoverynews.com)
  • CD40-ligand is a protein expressed on the surface of activated T lymphocytes that mediates T cell helper function. (tonixpharma.com)
  • CDC researchers have developed methods and adjuvants for enhancing a subject's immune response to respiratory syncytial virus (RSV) by inclusion of a CD40 binding protein. (nih.gov)
  • King Nazir Leon, age 2, has CD40 Ligand deficiency, a rare disease that affects his immune system and this disease is life-threatening. (saveoneperson.org)
  • Gray D, Dullforce P, Jainandunsing S. Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction. (bdbiosciences.com)
  • Since transgene appearance via replication-deficient adenovirus vectors in vivo is certainly low we've also built a conditionally replicating E1A-CR2 removed adenovirus expressing mutant Compact disc40L leading to significant amplification of ligand appearance and consequent improvement of its healing effect. (scienceexhibitions.org)
  • 1999) Enhanced levels of soluble and membrane bound CD40 ligand in patients with unstable angina: Possible reflection of T lymphocyte and platelet involvement in the pathogenesis of acute coronary syndromes. (scirp.org)
  • Treatment with the investigational CD40 ligand inhibitor frexalimab significantly reduced new MRI lesions i n relapsing multiple sclerosis (MS) by week 24, according to data presented at the joint meeting of the European Committee for Treatment and Research in Multiple Sclerosis (ECTRIMS) and the Americas Committee for Treatment and Research in Multiple Sclerosis (ACTRIMS). (newshealth.biz)
  • CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. (thermofisher.com)
  • The structure suggests that most of the hyper-IgM syndrome mutations affect the folding and stability of the molecule rather than the CD40-binding site directly. (nih.gov)
  • Despite the fact that the hyper-IgM syndrome mutations are dispersed in the primary sequence, a large fraction of them are clustered in space in the vicinity of a surface loop, close to the predicted CD40-binding site. (nih.gov)
  • In autosomal recessive hyper-IgM syndrome with CD40 mutation, manifestations are similar to those of the X-linked form. (merckmanuals.com)
  • Right here we show the fact that mutated cleavage-resistant type of Compact disc40L is a far more powerful inducer of apoptosis than wild-type ligand in Compact disc40-positive carcinoma cell lines. (scienceexhibitions.org)
  • TNX-1500 incorporates the antigen binding fragment (Fab) region of hu5c8, which has been extensively characterized including at the atomic level in complex with CD40-ligand 6 . (tonixpharma.com)
  • Thus CD40 activation can effect the key elements required for generation of antigen-specific cytotoxic T-cell responses. (scienceexhibitions.org)
  • CD40 is a key target because it has a unique role in initiating an antigen-specific immune response against tumors. (drugdiscoverynews.com)
  • Background CD40 and its own ligand (Compact disc40L) play a crucial function in co-ordinating immune system replies. (scienceexhibitions.org)
  • By stimulating different TNF signaling pathways, these HERA-ligands can increase the antitumor immune response. (drugdiscoverynews.com)
  • expressing a mutant Compact disc40L that's resistant to metalloproteinase cleavage in a way that ligand appearance is retained on the cell membrane. (scienceexhibitions.org)
  • Further based on our previous observation that Fas ligand mutated to resist cleavage from your cell membrane delivers a far more powerful apoptotic stimulus than wild-type FasL we've produced a mutant Compact disc40L that's resistant to cleavage by matrix metalloproteinases. (scienceexhibitions.org)
  • Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μl/well) can bind Human CD40, Fc Tag with a linear range of 0.156-5 μg/ml. (biovisi.com)
  • The newly published patent application includes claims related to proprietary anti-human CD40-ligand mAbs that were engineered to have modified effector function, including TNX-1500, which have reduced potential for Fc binding to FcγRII. (tonixpharma.com)