• Infection with the gram-negative bacterium Burkholderia pseudomallei can result in a life-threatening disease known as melioidosis. (health.mil)
  • Melioidosis is a potentially life threatening disease caused by the gram-negative bacterium Burkholderia pseudomallei . (health.mil)
  • There is no vaccine, and current treatments are hampered by the ability of the bacterium that causes the disease to resist even the strongest antibiotics.Hardy and lethal, that bacterium, Burkholderia pseudomallei, is classified by the Centers for Disease Control and Prevention as a potential bioweapon.UCLA-led research has identified two compounds that, based on tests on human cells and on mice, show potential for treating melioidosis. (medworm.com)
  • Research in the Brett laboratory is focused on elucidating the molecular mechanisms used by Burkholderia pseudomallei (melioidosis) and Burkholderia mallei (glanders) to evade clearance by host defenses. (unr.edu)
  • The main goal of our research is to identify correlates of antigen-induced immunity against B. pseudomallei and B. mallei and use this information to develop safe, affordable and effective melioidosis/glanders vaccines. (unr.edu)
  • Glanders is caused by infection with the bacterium Burkholderia mallei, and melioidosis is caused by Burkholderia pseudomallei. (centerforhealthsecurity.org)
  • The T2Biothreat Panel is a direct-from-blood molecular diagnostic test that runs on the FDA-cleared T2Dx® Instrument and simultaneously detects six biothreat pathogens, including the organisms that cause anthrax ( Bacillus anthracis), tularemia ( Francisella tularensis), glanders ( Burkholderia mallei), melioidosis ( Burkholderia pseudomallei ) , plague ( Yersinia pestis ), and typhus ( Rickettsia prowazekii ). (ozarksfirst.com)
  • The facultative, intracellular bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. (biomedcentral.com)
  • The genes that allowed the bacterium to survive in a soil environment, like genes that gave B. mallei the capacity to protect against bactericidals, antibiotics, and antifungals, were likely deleted. (wikipedia.org)
  • Glanders, a World Organisation for Animal Health (OIE)-listed disease, is a contagious, life-threatening disease of equids caused by the gram-negative bacterium Burkholderia mallei ( 1 ). (cdc.gov)
  • Burkholderia mallei is a Gram-negative, non-motile, aerobic bacterium belonging to the Burkholderiaceae family. (geneticpcr.com)
  • It is caused by infection with the bacterium Burkholderia mallei, usually by ingestion of contaminated feed or water. (kitpcr.com)
  • Burkholderia mallei was first called "Bacillus mallei" and was in the genus Pseudomonas until the early 1990s. (wikipedia.org)
  • Burkholderia mallei is an aerobic, Gram-negative, non-motile bacillus. (bvsalud.org)
  • B. mallei evolved from B. pseudomallei by selective reduction and deletions from the B. pseudomallei genome. (wikipedia.org)
  • Genome comparisons also seem to indicate that the B. mallei is still evolving and adapting to an intracellular lifestyle. (wikipedia.org)
  • The genome of B. mallei was sequenced in the United States by The Institute of Genomic Research. (wikipedia.org)
  • The genome for B. mallei is made up of two circular chromosomes. (wikipedia.org)
  • About 1000 B. pseudomellei genes are absent or varying in the B. mallei genome. (wikipedia.org)
  • A previous work conducted on single-nucleotide polymorphisms ( SNP ) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. (sciensano.be)
  • In this study, we designed a high-resolution melting ( HRM ) method for the screening of 15 phylogenetically informative SNP s within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. (sciensano.be)
  • 2004. Structural flexibility in the Burkholderia mallei genome . (umd.edu)
  • Molecular typing of Burkholderia mallei strains using Whole Genome Sequencing data. (canada.ca)
  • Many microbiologists are unfamiliar with B. mallei and as a result it has frequently been misidentified as a Pseudomonas species or as a contaminant in a culture. (wikipedia.org)
  • This research points to the importance of the continued investigation of the virulence mechanisms and potential countermeasures associated with B. mallei infections, including their Brazilian isolates. (bvsalud.org)
  • Multi locus sequence typing of Burkholderia pseudomallei isolates from India unveils molecular diversity and confers regional association in Southeast Asia. (cdc.gov)
  • 12/07/2015This 35-page report presents the results of an investigation to evaluate the persistence (or natural attenuation) of Yersinia pestis (Y. pestis), Francisella tularensis (F. tularensis), and Burkholderia mallei (B. mallei) on glass and soil under multiple environmental conditions and time points. (medworm.com)
  • Camels are also susceptible to B. mallei , as experimental infection has demonstrated ( 4 , 5 ). (cdc.gov)
  • In addition to horses and donkeys, dromedaries showed clinical signs of glanders, but B. mallei infection has not yet been confirmed. (cdc.gov)
  • Here we provide evidence for a B. mallei infection in 1 of the diseased dromedaries. (cdc.gov)
  • Additionally, evaluation of the immune effectors involved in the protective host response to B. mallei infection identified key cellular and humoral components. (tdl.org)
  • Confirmed Burkholderia mallei infection. (canada.ca)
  • It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia. (lookformedical.com)
  • This study aims to assess the virulence of a specific B. mallei strain, isolated in Brazil, in BALB/c mice through intranasal infection. (bvsalud.org)
  • B. mallei persisted within the liver and lung for five days post-infection and in the spleen for seven days. (bvsalud.org)
  • These diseases are caused by closely related Gram-negative bacteria named Burkholderia (B.) mallei and B. pseudomallei, respectively. (ufl.edu)
  • Burkholderia (B.) mallei is the causative agent of glanders. (sciensano.be)
  • Burkholderia mallei is the causative agent of glanders, a zoonosis listed by the World Group for Animal Well being as of obligatory notification. (flomedcorp.com)
  • This suggestion has found support from studies that compare strains of B. mallei to B. pseudomallei and indicate that their two respective genomes are very similar. (wikipedia.org)
  • The detailed mechanism(s) of Burkholderia mallei pathogenesis is virtually unknown, with the production of a polysaccharide capsule and presence of secretion systems as the only known mechanisms associated with virulence. (tdl.org)
  • Our laboratory has over 20 years of experience working with these important bacterial pathogens and has gained significant expertise in areas relating to Burkholderia genetics, pathogenesis, host-pathogen interactions, carbohydrate purification/characterization, glycoconjugate synthesis and the analysis of humoral/cellular immune responses. (unr.edu)
  • the Latin name of this disease (malleus) gave its name to the species causing it. (wikipedia.org)
  • Comparing the DNA of B. mallei and B. pseudomallei must be done at the 23S rDNA level, however, since no identifiable difference is found between the two species at the 16S rDNA level. (wikipedia.org)
  • In 1949, Walter Burkholder of Cornell University first described P cepacia (now known as Burkholderia cepacia ) as the phytopathogen responsible for the bacterial rot of onions. (medscape.com)
  • The BurMal dtec-qPCR comprises a series of specific targeted reagents designed for Burkholderia mallei detection by using qPCR. (geneticpcr.com)
  • The intracellular survival of B. mallei within murine macrophage J774.2 cells requires the type III secretion system (TTSS), although the effector protein(s) involved are unknown. (tdl.org)
  • The work presented herein identified an N-terminal portion of BopA sufficient to drive secretion throughout a TTS apparatus and characterized the potential virulence-associated functions of B. mallei TTS protein BopA to subvert the host cell and promote intracellular replication and survival utilizing an in vitro respiratory cell model. (tdl.org)
  • The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei. (sciensano.be)
  • Furthermore, in vivo studies assessed the attenuation of virulence offered by the B. mallei bopA mutant. (tdl.org)
  • Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. (elsevierpure.com)
  • These findings underscore the detectable virulence of the Brazilian B. mallei BAC 86/19 strain in mice, which are relatively resilient hosts. (bvsalud.org)
  • The strain, B. mallei BAC 86/19, was obtained from the tracheal secretion of a young mare displaying positive serology but no clinical signs of glanders. (bvsalud.org)
  • Natural B. mallei infections are known to occur in various mammals (e.g., cats, bears, wolves, and dogs). (cdc.gov)
  • Infections with bacteria of the genus BURKHOLDERIA . (lookformedical.com)
  • BopA, a predicted effector protein of B. mallei, shares 24% amino acid homology to the TTS effector IcsB of Shigella flexneri, which is responsible for intercellular spread and host cell invasion. (tdl.org)
  • Multilocus sequence typing has revealed that B. mallei most likely evolved from a B. pseudomallei clone reduction. (wikipedia.org)
  • B. mallei is very closely related to B. pseudomallei, being 99% identical in conserved genes when compared to B. pseudomallei. (wikipedia.org)
  • Multilocus variable number tandem repeat analysis of a Burkholderia mallei strain isolated from a diseased dromedary in Bahrain revealed close genetic proximity to strain Dubai 7, which caused an outbreak of glanders in horses in the United Arab Emirates in 2004. (cdc.gov)
  • It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin. (lookformedical.com)
  • Suspected B. mallei colonies were analyzed with the API 20 NE-test (bioMérieux, Marcy l'Etoile, France) and were positive for nitrate, glucose assimilation, arginine dehydrolase (after 4 days of incubation), N-acetyl glucosamine, and potassium gluconate. (cdc.gov)
  • The API 20 NE-test identified the colonies as B. mallei because the same API ID number (1140504) occurred as in the previously isolated Dubai 7 strain ( 1 ). (cdc.gov)
  • Thus, the reason that B. mallei is not found outside of a host is because it lacks the genes necessary for survival in the soil. (wikipedia.org)
  • Burkholderia pseudomallei grown on sheep blood agar for 96 hours. (health.mil)
  • B. mallei is responsible for causing glanders disease, which historically affected mostly animals, such as horses, mules, and donkeys, and rarely humans. (geneticpcr.com)
  • Additionally, previous reports have documented that B. mallei TTSS is required for phagosomal membrane lysis and bacterial escape into the macrophage cytoplasm. (tdl.org)
  • High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei. (sciensano.be)
  • B. mallei has a polysaccharide capsule which indicates its potential as a pathogen. (wikipedia.org)
  • While progress has been made in demonstrating the importance of the TTSS contribution to B. mallei pathogenicity, additional work is needed to identify the potential secreted (effector) molecule(s) involved. (tdl.org)
  • No standardised system exists for differentiating between B. mallei and B. pseudomallei. (wikipedia.org)
  • Because B. mallei is an obligate mammalian pathogen, it must infect a host mammal to live and to be transmitted from one host to another. (wikipedia.org)
  • B. mallei has been eradicated in most Western countries, but still affects animals in Africa, Asia, the Middle East, Central America, and South America. (geneticpcr.com)