• We then discuss exciting advances made using scRNA-seq in the study of branching morphogenesis and differentiation in mammalian organs, with emphasis on the lung, kidney, and mammary gland. (princeton.edu)
  • Finally, we highlight the importance of physical and mechanical signals in branching morphogenesis and speculate about how scRNA-seq and related techniques could be applied to study tissue morphogenesis beyond just differentiation. (princeton.edu)
  • One module of gene expression is consistent with the predicted distal differentiation trajectory and consists of genes related to processes important to lung development, including epithelial/respiratory system branching and development. (elifesciences.org)
  • We have shown that peripheral sensory nerves coordinate vascular branching pattern and arterial differentiation during the vascular remodeling. (nih.gov)
  • Studies have revealed that hypoxia-induced genes are important regulators of angiogenesis, although the precise mechanism by which they coordinate vascular branching and differentiation remain unclear. (nih.gov)
  • Varma S, Cao Y, Tagne JB, Lakshminarayanan M, Li J, Friedman TB, Morell RJ, Warburton D, Kotton DN, Ramirez MI (2012) The transcription factors Grainyhead-like 2 and NK2-homeobox 1 form a regulatory loop that coordinates lung epithelial cell morphogenesis and differentiation . (nih.gov)
  • Major program areas for the branch include developmental genetics, including genomic analysis of human structural birth defects, systems developmental biology, early embryonic development and differentiation, biophysics/biomechanics of development, developmental neurobiology and neural crest differentiation, organogenesis, regeneration, and regenerative medicine. (nih.gov)
  • Many organs, including salivary glands, lung and kidney, are formed during embryonic development by epithelial branching. (nih.gov)
  • We reviewed evidence on the impact of environmental chemicals on lung development and key signaling events in lung morphogenesis, and the relevance of potential outcomes to public health and regulatory science. (nih.gov)
  • Scholars@Duke publication: Bombesin and [Leu8]phyllolitorin promote fetal mouse lung branching morphogenesis via a receptor-mediated mechanism. (duke.edu)
  • We now demonstrate that GRP and GRP receptor genes are expressed in fetal mouse lung as early as embryonic day 12 (E12), when lung buds are beginning to branch. (duke.edu)
  • To explore the possibility that bombesin-like peptides might play a role in branching morphogenesis, E12 lung buds were cultured for 48 hr in serum-free medium. (duke.edu)
  • These observations suggest that BLPs secreted by pulmonary neuroendocrine cells may contribute to lung branching morphogenesis. (duke.edu)
  • The formation of the trachea consists of a series of developmental events, and its analysis provides an excellent model system for studying the morphogenesis of other branched structures, such as the vertebrate lung airways, circulatory system, kidney ducts, and excretory epithelia. (sdbonline.org)
  • We propose that the deletion of TIMP-3 disrupts the exquisite TIMP/MMP balance required for proper focal ECM proteolysis, which leads to correct bronchiole branching morphogenesis in the developing mouse lung. (uwo.ca)
  • Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. (nih.gov)
  • 19. BMP7 inhibits branching morphogenesis in the prostate gland and interferes with Notch signaling. (nih.gov)
  • This study was conducted to test the hypothesis that in utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure differentially inhibits branching morphogenesis and ductal canalization in the ventral, dorsal, lateral, and anterior mouse prostate. (nih.gov)
  • His work was recognized with the 2011 Morphological Sciences Award from the American Association of Anatomists for his contributions to the field of epithelial morphogenesis. (hopkinsmedicine.org)
  • Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. (nih.gov)
  • 10. Signaling pathways activated by epidermal growth factor receptor or fibroblast growth factor receptor differentially regulate branching morphogenesis in fetal mouse submandibular glands. (nih.gov)
  • Employing an in vitro organ culture system, we found decreased bronchiolar branching in null lungs when compared with wild type (WT) counterparts after 2 days in culture. (uwo.ca)
  • The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. (bioone.org)
  • Increased branching, vigorous vegetative growth and change in biochemical components are often noted in micropropagated plants acclimatized under ex vitro condition. (bioone.org)
  • By subcellular fractionations, protein interaction analyses, subcellular reconstitutions of protein complexes, colocalization studies in cells and tissues, and by functional analyses in neuronal morphogenesis we demonstrate that both proteins associate and work with each other closely. (jneurosci.org)
  • 8. Connexin 43 Is Necessary for Salivary Gland Branching Morphogenesis and FGF10-induced ERK1/2 Phosphorylation. (nih.gov)
  • 15. Salivary gland branching morphogenesis. (nih.gov)
  • 16. Involvement of hepatocyte growth factor in branching morphogenesis of murine salivary gland. (nih.gov)
  • Philippe Soriano ABSTRACT Loss of FGF signaling leads to defects in salivary gland branching, but the mechanisms underlying this phenotype remain largely unknown. (biologists.com)
  • 4. Multiple fibroblast growth factors support growth of the ureteric bud but have different effects on branching morphogenesis. (nih.gov)
  • For instance, like mammalian prostate, Drosophila MAG morphogenesis is marked by recruitment of fibroblast growth factor receptor (FGFR) - a signalling pathway often seen recruited for tubulogenesis - starting early during its adepithelial genesis. (biologists.com)
  • FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. (nih.gov)
  • Here we show that the extracellular matrix protein fibronectin is essential for cleft formation during the initiation of epithelial branching. (nih.gov)
  • 9. FGF alters epithelial competence for EGF at the initiation of branching morphogenesis of mouse submandibular gland. (nih.gov)
  • A null mutation for tissue inhibitor of metalloproteinases-3 (Timp-3) impairs murine bronchiole branching morphogenesis. (uwo.ca)
  • We have examined the role of TIMP-3 on ECM homeostasis and bronchiole branching morphogenesis during murine embryogenesis. (uwo.ca)
  • The insect tracheal system consists of an intricately branched system of tubules that provide oxygen throughout the animal. (sdbonline.org)
  • Decreasing the fibronectin concentration by using small interfering RNA and inhibition by anti-fibronectin or anti-integrin antibodies blocked cleft formation and branching. (nih.gov)
  • Exogenous fibronectin accelerated cleft formation and branching. (nih.gov)
  • Thus, fibronectin expression is required for cleft formation in branching morphogenesis associated with the conversion of cell-cell adhesions to cell-matrix adhesions. (nih.gov)
  • Cell and fibronectin dynamics during branching morphogenesis. (nih.gov)
  • Potential mechanisms include interference with highly conserved factors in developmental processes such as gene regulation, molecular signaling, and growth factors involved in branching morphogenesis and alveolarization. (nih.gov)
  • Although extensive knowledge about CNCCs has accumulated over last several decades, the mechanisms that control late-stage CNCC fate decisions during craniofacial morphogenesis remain to be elucidated 4 . (nature.com)
  • In addition, cells not expressing polycystin-2 exhibit significantly more branching morphogenesis and multicellular tubule formation under basal and hepatocyte growth factor-stimulated conditions than their polycystin-2-expressing counterparts, suggesting that polycystin-2 may also play an important role in the regulation of tubulogenesis. (nih.gov)
  • These experiments have opened up new areas of research into the regulation of branching morphogenesis and patterning by the metabolic microenvironment. (nih.gov)
  • In the early kidney development, a simple epithelial tube called ureteric bud is derived from the intermediate mesoderm and undergoes a complex process of growth and terminal bifid branching. (elsevierpure.com)
  • The branching of the ureteric bud is achieved by different cellular behaviors including cell proliferation and chemotaxis. (elsevierpure.com)
  • Cobl-mediated dendritic branch induction in hippocampal neurons critically relied on Abp1. (jneurosci.org)
  • He will continue fundamental research to define how dendritic branching patterns are controlled, using the fruit fly nervous system as a model. (nih.gov)
  • 12. Heparanase cleavage of perlecan heparan sulfate modulates FGF10 activity during ex vivo submandibular gland branching morphogenesis. (nih.gov)
  • In branching morphogenesis, repetitive epithelial cleft and bud formation create the complex three-dimensional branching structures characteristic of many organs. (nih.gov)
  • By in situ hybridization, GRP receptor transcripts were at highest levels in mesenchymal cells at cleft regions of branching airways and blood vessels. (duke.edu)
  • Branched organs are highly complex but typically develop recursively, such that a given developmental stage theoretically contains the entire spectrum of cell identities from progenitor to terminally differentiated. (princeton.edu)
  • Therefore, branched organs are a highly attractive system for study by scRNA-seq. (princeton.edu)
  • In addition, we discuss the possibilities and limitations for applying these techniques to studying branched organs. (princeton.edu)
  • Uncovering cellular networks in branching morphogenesis using single-cell transcriptomics. (princeton.edu)
  • Branching and fusion are complex cellular processes and pose a number of developmental questions. (sdbonline.org)
  • In utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in the C57BL/6J mouse prostate: lobe-specific effects on branching morphogenesis. (nih.gov)
  • Branching morphogenesis is an essential component of prostate development. (nih.gov)
  • TCDD inhibited branching morphogenesis in all prostate lobes. (nih.gov)
  • In this paper, we examine how the branching morphology depends on different physical or chemical factors by constructing a cell-based model to describe the simple tube branching in the early kidney development. (elsevierpure.com)
  • Similar to in situ coral gardening techniques where coral fragments of branching species are mass-produced in underwater nurseries, microfragmentation takes advantage of the corals' ability to reproduce asexually and is not limited to branching species. (frontiersin.org)
  • Patterned cell and matrix dynamics in branching morphogenesis. (nih.gov)
  • The branch also funds community resources, animal model systems, research tool development, and training to facilitate the efforts of the developmental biology research community. (nih.gov)
  • We show that the different branching patterns are accurately predicted by growth-chemotaxis ratio. (elsevierpure.com)
  • dys -RNAi experiments reveal tracheal fusion defects in the lateral trunk, dorsal branch, and ganglionic branch but not in the dorsal trunk. (sdbonline.org)
  • However, esg mutant embryos show an absence of dys expression in tracheal fusion cells in most branches, but not the dorsal trunk, further indicating branch-specific function of esg . (sdbonline.org)
  • Previous work showed that gastrin-releasing peptide (GRP), a major pulmonary bombesin-like peptide, occurred in neuroendocrine cells exclusively in branching human fetal airways. (duke.edu)
  • 1 Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892-4370, USA. (nih.gov)
  • We then seek to understand what environmental signals ensure the nerve-mediated arterial branching. (nih.gov)
  • Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing the cost of propagation, optimal plant production depends on better understanding of physiological and biochemical responses of plants to the signals of the culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. (bioone.org)
  • How do hypoxia-responsive factors such as CXCL12, VEGF-A and EPO regulate the vascular branching morphogenesis during neuro-vascular wiring formation? (nih.gov)
  • We are also interested in understanding photoreceptor morphogenesis and synapse formation. (nih.gov)
  • Here, we focus on single-cell investigations of branching morphogenesis. (princeton.edu)
  • The fusion process is mediated by a distinct fusion cell residing on each branch. (sdbonline.org)
  • The bombesin receptor antagonist [Leu13- psi(CH2NH)Leu14]bombesin alone (100 nM) had no effect on baseline branching but completely abolished bombesin-induced branching. (duke.edu)
  • We use established model organisms and genome-enabled species from many branches of the tree of life. (berkeley.edu)
  • The Developmental Biology and Congenital Anomalies Branch (DBCAB), formerly known as the Developmental Biology and Structural Variation Branch, focuses on the biological causes and consequences of structural birth defects. (nih.gov)
  • After the tracheal precursor cells divide and invaginate, they extend branches, and the branches from neighboring segments fuse to form the mature tracheal tree. (sdbonline.org)
  • When a synthetic inhibitor of MMPs at low dose was added to the culture system, branching was augmented regardless of genotype. (uwo.ca)
  • A-C ) Whole mount confocal imaging of embryonic lungs isolated from SOX9-GFP reporter mice at E12.5 ( A ), E13.5 ( B ), and E14.5 ( C ) show SOX9 expression at the distal branch tips and in the tracheal mesenchyme. (elifesciences.org)