Biology hek293 cells. Medical search. Frequent questions

Furthermore, we find that SET8 interacts with proliferating cell nuclear antigen through a conserved motif, and SET8 is required for DNA replication fork progression. (rupress.org)

Commonly known as HEK293 cells they are actually an excellent protein source, especially for secreted proteins. (uni-konstanz.de)
Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
Our long-range vision is to facilitate the creation of diagnostic and pharmaceutical products that are based on the emerging new field of protein methylation biology. (ch3biosystems.com)
Noto will pick up from Joseph Russo's (PhD biology 2014) previous findings of the Puf protein binding sites in LRRK2. (umsl.edu)
To investigate cellular and metabolic characteristics associated with these limitations, we compare HEK293 clones producing either erythropoietin (EPO) (secretory) or GFP (non-secretory) protein at different rates. (ulster.ac.uk)
The protein content of BIO-MPM-1 is therefore less than 30 mg/L, and the medium contains no growth factors or hormones other than insulin. (bioind.com)
CHO cells are most commonly used as a stable expression system and HEK293 cells are highly useful for transient gene expression where protein is harvested within a few days following DNA delivery. (sigmaaldrich.com)
Developing the project will boost my career as new skills in structural biology, including protein electron microscopy, will complement my previous expertise in protein-protein interaction and crosslinking mass spectrometry studies. (europa.eu)
Finally, we describe methods for the growth of these cells lines in scalable suspension culture for the preparation of milligram amounts of recombinant protein. (essex.ac.uk)
In the field of Cell biology, his study on Golgi apparatus and Transport protein overlaps with subjects such as Palmitoylation. (research.com)
His research related to Transport protein and Endosome might be considered part of Cell biology. (research.com)
His work in Colorectal cancer addresses issues such as Cell type, which are connected to fields such as Protein kinase A and Stromal cell. (research.com)
Altogether, this study defines a homeostatic mechanism that sustains peroxisomal protein import in cells with decreased ubiquitination capacity. (bvsalud.org)

Experience with the culture and transfection of mammalian cell lines such as HEK293, CHO, HeLa, Vero cells. (peccoud.org)
Therefore, a clearer understanding capsid expression was analyzed by immunofluorescent assay of WNV is necessary in order to identify new strategies to treat in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag or prevent this viral infection (3). (cdc.gov)

Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
The HEK 293 pattern most closely resembled that of adrenal cells, which have many neuronal properties. (wikipedia.org)
Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)

He has worked with adherent and suspension cells and operation in bioreactors, including HEK293, Vero cells, MDCK and insect cells. (vvectorbio.com)

2023 Jurkat-Lucia™ NFAT Cells for ADCC & ADCP Assays Hederman A.P. et al. (invivogen.com)
2023 Ramos-Blue™ Cells Akasaka D. et al. (invivogen.com)
2023 ACE2 expressing HEK293 cells Bianchini F. et al. (invivogen.com)
2023 THP1-Lucia™ ISG Cells Wang X. et al. (invivogen.com)
2023 THP1-Dual™ Cells Li J. et al. (invivogen.com)

Methods in Molecular Biology, vol 2457. (stfc.ac.uk)
Demonstrated proficiency with molecular biology techniques such as DNA purification, vector construction, cloning, and DNA fragment assembly methods. (peccoud.org)

Address correspondence to C. Borner, Institute of Molecular Medicine and Cell Research, Center for Biochemistry and Molecular Cell Research, Albert-Ludwigs-University Freiburg, Stefan-Meier Strasse 17, D-79104 Freiburg, Germany. (rupress.org)

Conversion of Peripheral Blood NK Cells to a Decidual NK-like Phenotype by a Cocktail of Defined Factors The Journal of Immunology, 190:3939-3948 (2013). (bioind.com)
It has been shown that inflammation caused by oxidized low-density lipoprotein (ox-LDL) contributes to the occurrence and development of AS [ 1 ], which can promote vascular smooth muscle cell (VSMC) migration, proliferation, and transformation from a contractile to a synthetic phenotype [ 2 - 6 ]. (hindawi.com)
The Transcriptome study combines topics in areas such as Phenotype, Regulation of gene expression, Neuroepithelial cell and Gene expression profiling. (research.com)

Human embryonic stem cells, for instance, are likely to have similar repair outcomes as mESCs," the research team writes. (bio-itworld.com)

Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. (uni-konstanz.de)
There are two main ways to express your gene in HEK293 cells: Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator. (uni-konstanz.de)
Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
Here, we utilize deep-coverage mass spectrometry to define the E1-E2 interactome and to determine the proteins that are modulated by knockdown of UBA1 and of each E2 in human cells. (bvsalud.org)

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
Graham performed the transfection a total of eight times, obtaining just one clone of cells that were cultured for several months. (wikipedia.org)
Learn more about the HEK293 Media Survey panel and discover your optimal media, and streamline the workflow process for viral vector production while increasing transfection efficacy and cell growth consistency. (insights.bio)
Streamline the workflow process for viral vector production while increasing transfection efficacy and cell growth consistency. (insights.bio)
In transient transfection experiments in T47D and 293T cells, the 1708E product was mislocalised to the cytoplasm and induced centrosome amplification in 293T cells. (elsevierpure.com)

Contrary to urban legend, they are significantly cheaper than insect cells to culture. (uni-konstanz.de)
contrary to insect cells, the DNA can be used directly for transient transfections . (uni-konstanz.de)

2016 ). This study was conducted to analyze the anticancer effects of green-synthesized AgNPs using Juniperus polycarpos on A549 (adenocarcinomic human alveolar basal epithelial cells) cell lines, as well as comparing it with Cisplatin commercial drug. (springeropen.com)

She teases about her enthusiasm for science, but the senior biology major at the University of Missouri-St. Louis landed herself a College of Arts and Sciences Undergraduate Research Grant to study one of the genes often overexpressed in Parkinson's patients. (umsl.edu)
Our results offer potential target pathways and genes for further development of the secretory power in mammalian cell factories. (ulster.ac.uk)
The team discovered that cells often repair broken genes in ways that are precise and predictable, sometimes even returning mutated genes back to their healthy version. (bio-itworld.com)
For Kotter and bit.bio, the company views cells as if they are a computer with the nucleus and genes acting like the hard drive and computer programs that operate within. (ddw-online.com)
We stably engineered O-glycan sulfation capacities in HEK293 cells by site-directed knockin of sulfotransferase genes in combination with knockout of genes to eliminate endogenous O-glycan branching (core2 synthase gene GCNT1) and/or sialylation capacities in order to provide simplified substrates (core1 Galβ1-3GalNAcα1-O-Ser/Thr) for the introduced sulfotransferases. (lu.se)

Next, we describe the assembly of this expression system into HEK293S cells and a derivative cell line devoid of complex N-glycosylation. (essex.ac.uk)
The study incorporates disciplines such as Cell growth, Glycosylation, Cell membrane, Enzyme and Glycosphingolipid in addition to Golgi apparatus. (research.com)

This study was performed on neonatal rat cardiomyocytes to study toxin effects on various fundamental aspects of cardiac muscle cells activity: cell beating, intracellular Ca2+ and cAMP levels, cell vitality, mitochondrial membrane potential and type of cell death occurrence. (units.it)

For recombinant antibody production, stable cell lines such as the Chinese hamster ovary (CHO) cells, human embryonic kidney 293 (HEK293), or NS0 murine myeloma cells are more commonly employed. (sigmaaldrich.com)

2021 ) Optimisation of Neuraminidase Expression for Use in Drug Discovery by Using HEK293-6E Cells. (academictree.org)

2018 ). It was also confirmed that AgNPs synthesized by the Juniperus chinensis leaf showed the potent anticancer effect on the human adenocarcinoma gastric (AGS) cell lines (Al-Dhafri and Ching 2019 ). (springeropen.com)

We show that small interfering RNA inhibition of SET8 expression leads to decreased cell proliferation and accumulation of cells in S phase. (rupress.org)
In normal cells, Pufs and miRNAs act to regulate low levels of expression of this gene. (umsl.edu)
For integration into the FRT docking site, cells were co-transfected with the FLP expression vector pCSFLPe and the corresponding integration vector at a ratio of 9 to 1. (healthykidneyclub.com)
For conditional expression of transgenes, cells were cultured in 1 g/ml doxycycline or 1 M Shld1. (healthykidneyclub.com)
In contrast, a study revealing whether a change in the expression level of DNMT enzymes in the cells affects klotho gene expression has not been found. (tubitak.gov.tr)
For this purpose, the expression levels of DNMT3A and DNMT3B in HEK293 cells were artificially changed, and the effect on klotho gene promoter activity was investigated using a reporter gene. (tubitak.gov.tr)
With this technology, the problems of cell line drift, antibody expression variations, and antibody sequence mutations associated with classical hybridoma production and storage can be avoided. (sigmaaldrich.com)
Recombinant antibody production from most species consist of a heavy and a light chain and it is important to note that to accomplish maximum IgG production in mammalian cells, one must achieve a balanced expression of both heavy and light chains. (sigmaaldrich.com)
However, expression of these two chains on separate vectors is not an efficient way to generate monoclonal antibody producing cell lines. (sigmaaldrich.com)
First, we will outline the steps for establishing a tightly regulated gene expression system in human HEK293S cells. (essex.ac.uk)
Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
His scientific interests lie mostly in Cell biology, Signal transduction, Genetics, Regulation of gene expression and Gene. (research.com)
His Computational biology study incorporates themes from HEK 293 cells, Cell culture, Gene expression, Automated reasoning and Chromosome conformation capture. (research.com)
His Computational biology study combines topics from a wide range of disciplines, such as Transcriptome, Regulation of gene expression, Gene, Drug and Gene regulatory network. (research.com)
Nils Blüthgen combines subjects such as Systems biology, Gene expression and Cell fate determination with his study of Cell biology. (research.com)
His main research concerns Transcriptome, Computational biology, Cell biology, MAPK/ERK pathway and Gene expression. (research.com)
He is also experienced in cell line development and with the P. pastoris yeast expression system. (vvectorbio.com)
Expression of the galactose 3-O-sulfotransferase 2 in HEK293 cells resulted in sulfation of core1 and core2 O-glycans, whereas expression of galactose 3-O-sulfotransferase 4 resulted in sulfation of core1 only. (lu.se)

As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
His Cell membrane research integrates issues from Endocytosis, Secretion assay and Focal adhesion. (research.com)
His Microtubule study integrates concerns from other disciplines, such as Focal adhesion, Exocytosis, Secretion assay, Secretion and Cell membrane. (research.com)
DVTP nick-end labeling (TUNEL) assay with the In Situ Cell determined according to the manufacturer's protocol. (cdc.gov)
Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)

Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973. (wikipedia.org)

In keeping with these results p45 inhibits FasL-induced cell loss of life markedly. (healthyconnectionsinc.com)

The effect of 10 μM SNP was reinforced on proliferation (from 13% to 90%) and elevation of cGMP levels (from 7.6 to 13.5 pmol/10 7 cells). (scirp.org)
Garg, U.C. and Hassid, A. (1989) Nitric Oxide-Generating Vasodilators and 8-Bromo-cyclic Guanosine Monophosphate Inhibit Mitogenesis and Proliferation of Cultured Rat Vascular Smooth Muscle Cells. (scirp.org)
However, a function for SET8 in mammalian cell proliferation has not been determined. (rupress.org)
Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line. (springeropen.com)

Eukaryotic cells repair the DSBs by the fast but potentially mutagenic Non-Homologous End Joining (NHEJ), and by the accurate Homology Directed Repair (HDR) pathways. (europa.eu)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)

A team of researchers from Weill Cornell Medicine and Mount Sinai Hospital developed two novel models of humanized mice using five human embryonic stem cell lines. (healthykidneyclub.com)
Four human embyonic stem cell lines were used in the experiment. (healthykidneyclub.com)
In addition, the HEK293 human embryonic kidney stem cell line was also used. (healthykidneyclub.com)
His Transcriptome study combines topics in areas such as Exome sequencing, Proteome, Stem cell and Induced pluripotent stem cell. (research.com)
Based in Cambridge, UK, bit.bio is a synthetic biology company that has developed a technology to program induced pluripotent stem cells (iPSCs) into any human cells of choice. (ddw-online.com)
The company's proprietary opti-ox (optimised inducible overexpression) technology is what bit.bio uses to execute these genetic programs in human stem cells. (ddw-online.com)
At the recent International Society of Stem Cell Research (ISSCR) conference in Boston, Kotter presented the company's latest consistency data from three different cell types reprogrammed with opti-ox, and these data confirm that bit.bio is truly making the manufacture of human cells an industrial process. (ddw-online.com)

In the present study human embryonic kidney (HEK 293) cells were used to study the growth after repeated exposure to SNP. (scirp.org)
The formulation contains no albumin, which has been found to be non-essential for cell growth, and even prevents efficient adhesion in some cases. (bioind.com)
Designed to improve growth and production of AAV and AAV2 serotypes, the HSMP optimizes HEK293 media allowing for increased titers and improved VG/Capsid ratios. (insights.bio)
His studies deal with areas such as Epidermal growth factor and HRAS as well as Cell biology. (research.com)

Human embryonic kidney (HEK293) cells are the most commonly used cell line to produce viral vectors for gene therapy. (insights.bio)

Choosing the proper media in a scale-down model for each cell line and process can improve yield and lower production costs as well as managing to the demand for high demand and dosage of the therapeutics. (insights.bio)
A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. (cdc.gov)

Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. (mdpi.com)
Exocytosis and related Cell and Extracellular. (research.com)

293T (or HEK 293T) is a derivative human cell line that expresses a mutant version of the SV40 large T antigen. (wikipedia.org)
After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK 293 line. (wikipedia.org)
However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
An embryonic adrenal precursor cell therefore seems the most likely origin cell of the HEK 293 line. (wikipedia.org)
The first reference to the cell line as "293T" may be its use to create the BOSC23 packaging cell line for producing retroviral particles. (wikipedia.org)
The cell line was developed by J.P. Jacobs in September 1966 after an abortion was committed on a physically healthy 27-year-old woman for psychiatric reasons. (healthykidneyclub.com)
Eminence is committed to developing and manufacturing high-quality CHO chemically defined media and providing tailor-made cell line and media formulation development services to clients around the globe working in the life science industry. (bio.org)
To explore further the genetic and biosynthetic regulation of sulfated O-glycans, here we expanded a cell-based glycan array in the human embryonic kidney 293 (HEK293) cell line with sulfation capacities. (lu.se)
Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. (cdc.gov)

Given the location of the adrenal gland (adrenal means "next to the kidney"), a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. (wikipedia.org)

The study incorporates disciplines such as Pharmacogenomics, Cancer cell, Proteomics, Biomarker and Drug in addition to Computational biology. (research.com)

J Cell Biol (2004) 164 (7): 1021-1032. (rupress.org)
J Cell Biol (2007) 179 (7): 1337-1345. (rupress.org)
J Cell Biol. (ucsb.edu)

These cells can be provided to companies and scientists involved in disease research and drug discovery. (ddw-online.com)

bit.bio has discovered a way to utilise genomic safe harbour sites, which are areas of the genome that enable researchers to reprogram cells without the programs being silenced. (ddw-online.com)
Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)

After the addition of 2mM glutamine, BIO-MPM-1 Medium is ready to use for adherent cells. (bioind.com)
Z. Aberman, Adherent cells from placenta and use of same in disease treatment . (bioind.com)

a good tool to do that is the Flp-In system from Invitrogen that can be used to generate highly expressing stable cell lines. (uni-konstanz.de)
In this chapter, we focus on the construction of stable mammalian cell lines to overcome this hurdle. (essex.ac.uk)

His Computational biology research incorporates themes from Serous ovarian cancer and Drug. (research.com)

To help cell and gene therapy product manufacturers discover the optimal HEK293 media for their viral vector-based applications, FUJIFILM Irvine Scientific has developed the HEK293 Media Survey Panel (HSMP). (insights.bio)
Hamid Soleymani, Scientist II- Cell and Gene Therapy Group, at Fujifilm Irvine Scientific is a part of the Life Science R&D team and supports the company's efforts to develop new or improved products, process improvements, and technical support for Cell and Gene Therapies. (insights.bio)

Materials and Strategies Cell cultures HEK293 cells had been cultured in the DMEM moderate supplemented with 10% fetal leg serum 100 systems/ml penicillin and 100 μg/ml streptomycin. (healthyconnectionsinc.com)

To evaluate MAPKs pathway, p-p38 and p-JNK were assessed by western blot analysis on peripheral blood mononuclear cells. (bvsalud.org)

The acute and chronic effects of sodium nitroprusside (SNP) are well characterized for vascular smooth muscle cells (VSMC). (scirp.org)
This study aimed to identify the role of AA in oxidized low-density lipoprotein (ox-LDL)-induced vascular smooth muscle cell (VSMC) behaviours and to explore the effects of microRNAs (miRNAs). (hindawi.com)

The host laboratory of Prof. Montoya already focuses on improving the properties of the Cas nucleases by means of structural biology and the Shieldin project fits right in their research portfolio. (europa.eu)

His research links Computational biology with Gene. (research.com)
Computational biology, Cell biology, Signal transduction, MAPK/ERK pathway and Genetics are his primary areas of study. (research.com)
His primary areas of study are Cancer research, Transcriptome, Computational biology, Colorectal cancer and KRAS. (research.com)

A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands , cell lines , selective antibiotics , antimicrobial agents , vaccine adjuvants , and antibodies . (invivogen.com)
As the transgenes are integrated at the same chromosomal site, cell lines were prepared by pooling individual colonies. (healthykidneyclub.com)
Cell lines with less than 5% blue cells were used for further experiments. (healthykidneyclub.com)
There are multiple aborted fetal cell lines currently in use, and each one can be traced back to a preborn baby. (healthykidneyclub.com)
With a dedicated team and proprietary technology, Eminence has commercialized products for CHO-K1, CHO-S, CHO-DG44, HEK 293 and other cell lines, supporting and collaborating with clients to improve the performance. (bio.org)
Some examples include industrial cell lines such as Chinese hamster ovary (CHO) cells and human embryonic kidney (HEK) 293 cells, having reduced long-term yields during manufacturing due to silencing 1 . (ddw-online.com)
Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
We address various cell culture systems along with different cell lines and how these cell culture systems can be used to provide better tools for HBV and HDV studies. (cdc.gov)

Transfected cells had been gathered and lysed in RIPA buffer (150 mM NaCl 1 NP-40 0.5% DOC 0.1% SDS 50 mM Tris pH 8.0). (healthyconnectionsinc.com)

and Richard Sherwood of Brigham and Women's Hospital-suggests that the cell's own repair mechanisms could one day be combined with CRISPR-based therapies that correct gene mutations by simply cutting DNA precisely and allowing the cell to naturally heal the damage ( https://doi.org/10.1038/s41586-018-0686-x ). (bio-itworld.com)

A Postdoctoral Research Associate position is available in the Department of Chemical and Biological Engineering to support a research program aiming at using methods from synthetic biology to rationally design gene delivery systems for biomedical applications. (peccoud.org)
ACS synthetic biology. (wustl.edu)
Notable therapeutic modalities being advanced by synthetic biology include immunotherapy, CRISPR therapy, and tumour targeting therapies like Chimeric Antigen Receptor (CAR-T) cells. (ddw-online.com)
As such, synthetic biology is playing a vital role in the production of treatments for a range of conditions and hard- to-treat diseases. (ddw-online.com)
Mark Kotter, Founder and CEO of bit.bio defines synthetic biology as "the engineering of biology," but states that in order to do this you want to "enter a software paradigm. (ddw-online.com)
Editing and executing code has never been particularly challenging in things like genetically engineered E. coli or yeast, two strains that have been used as foundational chassis for synthetic biology research and which have contributed to the development of small molecules and biopharmaceuticals. (ddw-online.com)
The synthetic biology platform is able to express transcription factor combinations that reprogram human iPSCs into highly defined and mature human cell types. (ddw-online.com)

Franck Perez focuses on Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
His primary scientific interests are in Cell biology, Golgi apparatus, Secretory pathway, Endoplasmic reticulum and Cancer research. (research.com)
His primary areas of investigation include Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)

As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
These results show a very cell-specific response to YTX if compared to previous studies, and a severe damage to in vitro cardiomyocytes. (units.it)
RD cells transfected with pcWNV-Cp-DJY or pcDNA3.1 sis in vitro through the mitochodrial/caspase-9 pathway. (cdc.gov)
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)
In vitro cell culture models have evolved with significant improvements over several decades. (cdc.gov)

We report that the WNV capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)

Senior biology major Christine Noto studies human neural cells for her research on Parkinson's gene LRRK2, for which she won a College of Arts and Sciences Undergraduate Research Grant. (umsl.edu)
Every day she goes into the Cell Culture Facility at UMSL to attend to human neural cells, specifically the HEK293 cells and SH-SY5Y cells. (umsl.edu)
Only her arms, sterilized with ethanol nearly 15 times each session and gloved, enter the vented sterile hood allowing her access to the neural cells. (umsl.edu)

Methods Cell. (bioind.com)
In traditional methods, monoclonal antibodies are produced by fusion of B cells with an immortal cell to produce a hybridoma that produces many copies of the exact same antibody. (sigmaaldrich.com)
U2OS.EGFP cells caused by inactivating frameshift insertion/deletion (indel) mutations introduced by error prone non-homologous end-joining (NHEJ) repair of nuclease-induced double-stranded breaks (DSBs) (Fig. 1a and Methods ). (cdc.gov)

AgNPs are utilized for treating different types of cancerous cells due to their extraordinary biomedical features (anti-cancer, anti-bacterial, anti-viral, and anti-angiogenic properties) as well as surface-specific characteristics (Burdușel et al. (springeropen.com)

How Does the Cell Membrane Function? (lifewithvernonhoward.com)
Modelling the role of membrane mechanics in cell adhesion on titanium oxide nanotubes. (ki.si)
The various areas that he examines in his Cell biology study include Secretion and Cell membrane. (research.com)
From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)

Anatomy16

Organisms13

Diseases5

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes79

Disciplines and Occupations32

Anthropology, Education, Sociology and Social Phenomena7

Technology, Industry, Agriculture6

Humanities6

Information Science22

Named Groups1

Health Care5

Proliferating cell nu1

Protein13

HeLa2

Neuronal4

Vero1

20235

Molecular Biology2

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Embryonic stem1

Proteins6

Transfection6

Insect cells2

Epithelial cells1

Genes5

Glycosylation2

Intracellular1

Murine1

20211

20191

Expression18

Assay5

Human embryonic kidney cells1

Inhibits1

Proliferation4

Eukaryotic Cells2

Stem7

Growth4

Commonly1

Therapeutics2

Extracellular2

Line9

Derived culture1

Study incorporates disciplines1

Biol3

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Genome2

Adherent2

Stable2

Research incorporates themes1

Gene therapy2

Cultures1

Peripheral1

Vascular smooth2

Structural Biology1

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Lines9

Gathered and lysed1

Https1

Synthetic7

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Membrane4