Biol cell cycle proteins. Medical search. Frequent questions

J Biol Chem 2004;279:44497-503. (uclahealth.org)
Small heat shock protein αB-crystallin is part of cell cycle dependent Golgi reorganization J Biol Chem 2004;279:43374-7. (uclahealth.org)
J Biol Chem 2000;275:17154-9. (uclahealth.org)
J Biol Chem. (karmanos.org)
J Biol Chem, 2009 Aug 28. (nih.gov)

Progression to malignancy is associated with the expression of viral proteins whose primary function is to drive virus replication by regulating cell proliferation, apoptosis and the recognition of infected cells by the immune system. (ki.se)
Cancer genetics, DNA damage response, apoptosis, cell cycle My current research is focused on two transcription factors that respond to DNA damage and play important roles in regulating tumor development. (mdanderson.org)
The first project focuses on E2F1, a regulator of genes important for cell cycle progression and apoptosis. (mdanderson.org)
Antagonists of anaphase-promoting complex (APC)-2-cell cycle and apoptosis regulatory protein (CARP)-1 interaction are novel regulators of cell growth and apoptosis. (karmanos.org)
In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
Taken together, our findings indicate that SS18-SSX-mediated attenuation of an EGR1-PTEN network regulates synovial sarcoma cell survival, and that HDAC inhibitor-mediated apoptosis operates at least in part through reactivation of this pathway. (nature.com)
Recruitment of BAG2 to DNAJ-PKAc scaffolds promotes cell survival and resistance to drug-induced apoptosis in fibrolamellar carcinoma. (ucsf.edu)
Chronic ER stress promotes apoptosis, at least in part through the UPR-induced transcription factor C/EBP homologous protein (CHOP). (jci.org)
These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
Protein phosphatase 4 (PP4) is a crucial protein complex that plays an important role in DNA damage response (DDR), including DNA repair, cell cycle arrest and apoptosis. (molcells.org)
To prevent these detrimental consequences, eukaryotic cells have evolved an elaborate and complex system, so called DNA damage response (DDR) comprising of DNA DSB repair, cell cycle arrest, apoptosis, and transcriptional regulation ( Jackson and Bartek, 2009 ). (molcells.org)
Binding of copper is a mechanism of homocysteine toxicity leading to COX deficiency and apoptosis in primary neurons, PC12 and SHSY-5Y cells. (medscape.com)

Cell proliferation and DNA replication defects in a Drosophila MCM2 mutant. (wikigenes.org)
The staurosporine analogues, K-252a and RK-286C, were found to cause DNA re-replication in rat diploid fibroblasts (3Y1) without an intervening mitosis, producing tetraploid cells. (rupress.org)
These results suggest that a putative protein kinase(s) sensitive to K-252a plays an important role in the mechanism for preventing over-replication after completion of previous DNA synthesis. (rupress.org)
We are interested in understanding at the molecular level how recombination occurs and what roles are played by the many proteins involved in DNA recombination, repair and replication. (brandeis.edu)
We are interested in determining what are the specific biochemical roles played by the many proteins implicated in DNA recombination, repair and replication. (brandeis.edu)
We have identified the proteins necessary to carry out the initial steps in strand invasion and the beginning of new DNA synthesis, which is significantly different from the normal process of replication. (brandeis.edu)
Moreover, we show that the Polycomb protein BMI1 interacts with CDC6, an essential regulator of DNA replication in eukaryotic cells. (plos.org)
Finally, we demonstrate that Polycomb proteins and associated epigenetic marks are crucial for the control of the replication timing of the INK4a/ARF locus during senescence. (plos.org)
Upon senescence, Jmjd3 is overexpressed and the MLL1 protein is recruited to the locus provoking the dissociation of Polycomb from the INK4/ARF locus, its transcriptional activation and its replication during early S-phase. (plos.org)
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
Changing protein-DNA interactions promote ORC binding-site exchange during replication origin licensing. (yeastgenome.org)
DNA replication origins retain mobile licensing proteins. (yeastgenome.org)
A Role of hIPI3 in DNA Replication Licensing in Human Cells. (yeastgenome.org)
Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. (yeastgenome.org)
Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae. (yeastgenome.org)
The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
Two yeast strains were used, differing in the biogenesis of peroxisomes, the organelles supplying the membranous vesicular environment in which CymRSV RNA replication takes place in infected plant cells. (microbiologyresearch.org)
Double-labelled immunofluorescence showed that both p33 and p92 replicase proteins localized to peroxisomes, independently of one another and of the presence of the replication template. (microbiologyresearch.org)
DI RNA replication occurred in yeast cells, as demonstrated by the presence of monomers and dimers of positive and negative polarities. (microbiologyresearch.org)
DI RNA replication also took place in yeast cells devoid of peroxisomes. (microbiologyresearch.org)
It is suggested that replication in these cells was targeted to the endoplasmic reticulum. (microbiologyresearch.org)

The peroxisomal PTS1-Import defect of PEX1-deficient cells is independent of pexophagy in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)
In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)
We have previously shown that budding yeast Mak11, whose homologues in other eukaryotes were described as modulating a p21-activated protein kinase function, accumulates in Rlp24-associated pre-60S complexes when their maturation is impeded in Saccharomyces cerevisiae. (pasteur.fr)

By acting as a Trojan horse for cell invasion, viruses have taught us many of the basic principles that regulate cell proliferation, differentiation and death. (ki.se)
Selective inhibitors of nuclear export block pancreatic cancer cell proliferation and reduce tumor growth in mice. (karmanos.org)
Cellular senescence is a fundamental cellular program that is activated after a finite number of cell divisions and operates to avoid further cell proliferation. (plos.org)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
We also aimed to evaluate the expression of TGF-β1 and p27 in the context of other cell cycle and proliferation markers such as cyclin D1 and Ki-67. (biomedcentral.com)
Growth factors do not only stimulate cell proliferation, but they may also act as growth inhibitors, depending on the cell type and the stimulatory pathway that is involved. (biomedcentral.com)
It has been reported that TGF-β influence different cell functions, including growth, proliferation and differentiation. (biomedcentral.com)
Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S. cerevisiae , causes proliferation of the endoplasmic reticulum membrane. (microbiologyresearch.org)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
1980), lates cell proliferation in the rat respiratory tract (Roemer et al. (cdc.gov)

1998). p21(WAF1) is required for butyrate-mediated growth inhibition of human colon cancer cells. (nature.com)

Genome Biol. (nature.com)

The translocation and the current potentiation were abolished in the presence of an A-kinase anchoring protein (AKAP) inhibitor. (jneurosci.org)
The SYT protein involved in the t(X;18) synovial sarcoma translocation is a transcriptional activator localised in nuclear bodies. (nature.com)
de Marcos-Lousa C, P Sideris D, Tokatlidis K. (2006) Translocation of mitochondrial inner-membrane proteins: conformation matters. (forth.gr)
2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)

Purification of APP-AT protein complexes and identification of the constituents by mass spectrometry revealed several previously reported APP-interacting proteins as well as proteins involved in synaptic maintenance. (jneurosci.org)
When cells enter senescence the binding to RD of both PRC1 and PRC2 complexes is lost leading to a decreased level of histone H3K27 trimethylation (H3K27me3). (plos.org)
Identifying conserved protein complexes between species by constructing interolog networks. (yeastgenome.org)
The peroxisomal protein import machinery, which shares similarities with chloroplasts, is unique in transporting folded and large (up to 10 nm in diameter) protein complexes into peroxisomes. (tcdb.org)

p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer. (biomedcentral.com)
Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)

2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
Mol Biol Rep;50(11): 9479-9496, 2023 Nov. (bvsalud.org)
Cell Reports, Available online 14 November 2023. (lu.se)

The Menkes protein is synthesized as a single-chain polypeptide localized to the trans-Golgi network in cells. (medscape.com)
As the copper is concentrated into vesicles for excretion from the cell, the cytosolic copper concentration decreases and ATP7A returns to the trans-Golgi network. (medscape.com)
Golgi fragmentation is a highly regulated process that allows division of the Golgi complex between the two daughter cells. (biologists.com)
The mitotic reorganization of the Golgi is accompanied by a temporary block in Golgi functioning, as protein transport in and out of the Golgi stops. (biologists.com)
Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells. (kegg.jp)

Eur J Cell Biol 1999;78:143-50. (uclahealth.org)

We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)
We have been fascinated by the process of yeast mating-type gene switching, in which cells replace about 700 bp of Ya or Y-specific DNA sequences at the MAT locus by recombining with one of two donor loci, called HMLDescription: image3 and HMRa. (brandeis.edu)
The functional inactivation of WD40 repeat protein Mak11 interfered with the 60S rRNA maturation, led to a cell cycle delay in G(1), and blocked green fluorescent protein-tagged Rpl25 in the nucleoli of yeast cells, indicating an early role of Mak11 in ribosome assembly. (pasteur.fr)

Similar current changes after PKA activation were obtained from human embryonic kidney 293t cells transfected with the wild-type heat transducer protein vanilloid receptor 1 (VR-1). (jneurosci.org)
Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
The ETS domain transcription factor ELK1 directs a critical component of growth signaling by the androgen receptor in prostate cancer cells. (karmanos.org)
Glucocorticoid receptor status is a principal determinant of variability in the sensitivity of non-small cell lung cancer cells to pemetrexed. (karmanos.org)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
Phosphorylation of the receptor protein Pex5p modulates import of proteins into peroxisomes. (ruhr-uni-bochum.de)
The deubiquitination of the PTS1-import receptor Pex5p is required for peroxisomal matrix protein import. (ruhr-uni-bochum.de)

Chromosome association of minichromosome maintenance proteins in Drosophila endoreplication cycles. (wikigenes.org)

Losartan has protective effect on aortic wall of rats with aortic dissection, and the mechanism may be through inhibiting transforming growth factor-beta 1 pathway, which activates downstream phosphoinositide 3-kinase/protein kinase B pathway related proteins, and then inhibits the expansion and progression of aortic dissection. (ijpsonline.com)
The progression from insulin resistance to type 2 diabetes is caused by the failure of pancreatic β cells to produce sufficient levels of insulin to meet the metabolic demand. (jci.org)
PP4IP interacts with PP4 complex, which is affected by DNA damage and cell cycle progression and decreases the dephosphorylational activity of PP4. (molcells.org)
The unnotified or undifferentiable early stages of oral squamous cell carcinoma (OSCC) progression are the prime reasons for late-stage detection and poor survival outcomes of oral cancer . (bvsalud.org)

This gene encodes a ribosomal protein that is a component of the 60S subunit. (wikipedia.org)
The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity. (microbiologyresearch.org)

From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)
Transmembrane and intracellular signal transduction mechanisms are the focus of our group, especially understanding how extracellular stimuli control cell growth and division, cell morphology, and gene expression at the biochemical level. (berkeley.edu)
GSE76211) ( 3 , 4 ), revealing a significantly upregulated gene in BCa tissues, the lysosomal-associated protein multispanning transmembrane 5 (LAPTM5). (spandidos-publications.com)
A large pore is formed by transmembrane proteins. (tcdb.org)

AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)

We have shown that this regulation involves the action of a small Recombination Enhancer (RE) sequence that enables a donor on the left chromosome arm to recombine preferentially in MATa cells. (brandeis.edu)
Regulation of peroxisomal matrix protein import by ubiquitination. (ruhr-uni-bochum.de)
In this study, we report a central role of the transcription factor, early growth response-1 (EGR1), in the regulation of HDAC inhibitor-induced apoptotic cell death in synovial sarcoma. (nature.com)
The functional importance of PP4 in cells is evident by the need of tight regulation of its activity required to maintain cell health. (molcells.org)
Hormonal regulation of the Menkes and Wilson copper-transporting ATPases in human placental Jeg-3 cells. (medscape.com)

For example, prostaglandins activate G s -protein-coupled receptors and initiate cAMP- and protein kinase A (PKA)-mediated processes. (jneurosci.org)
The pheromone receptors have seven hydrophobic segments and are coupled to a heterotrimeric G protein. (berkeley.edu)
Cell surface distribution of lectin receptors determined by resonance energy transfer. (uconn.edu)
Recent studies denoted that alterations in growth factors and growth factor receptors seem to influence the biologic behaviour of pancreatic cancer cells [ 2 ]. (biomedcentral.com)
Transforming growth factor-β [TGF-β] is such an example, being a growth stimulator in fibroblastic cells with TGF-β receptors, but a negative regulator in epithelial cells. (biomedcentral.com)
An abundance of soluble N-ethylmaleimide-sensitive factor adaptor protein receptors. (kegg.jp)

Bhat SP, Hale IL, Matsumoto B, Elghanayan D. Ectopic Expression of αB-crystallin in Chinese Hamster Ovary cells suggests a nuclear role for this protein. (uclahealth.org)
Hatzis P, Kyrmizi I, Talianidis I. (2006) Mitogen-activated protein kinase-mediated disruption of enhancer-promoter communication inhibits hepatocyte nuclear factor 4alpha expression. (forth.gr)
Intrinsically disordered regions in Pex13 and Pex5 resemble those found in nuclear pore complex proteins. (tcdb.org)

ATP-driven processes of peroxisomal matrix protein import. (ruhr-uni-bochum.de)

We study the mechanism by which EBV proteins expressed in malignant cells modify the cellular environment and regulate the interaction of the infected cells with the host immune system. (ki.se)

We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
Thus, the dephosphorylation of target proteins by PP4 is equally important in DDR as much as phosphorylation by kinases. (molcells.org)

It is suggested that these proteins are sorted initially from the cytosol to the endoplasmic reticulum and then to peroxisomes. (microbiologyresearch.org)

2006) Senseless physically interacts with proneural proteins and functions as a transcriptional co-activator. (forth.gr)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
11. Amsel, A. & Roussel, J. Motivational properties of ences in post-transcriptional processes spark interest in the development of frustration: I. Effect on a running response of the (protein redistribution, degradation), pharmacotherapies that selectively reg- addition of frustration to the motivational com- plex. (lu.se)

Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
In order to declare a major in Biology, Biomedical Sciences, or MBBT, a minimum GPA of 2.0 is required in the first 12 sh of BIOL taken at NPU, 4 sh of which must be BIOL 2200 or above. (northpark.edu)

Hardman et al found that insulin and estrogen increased the level of MNK mRNA and protein levels in the placenta. (medscape.com)
In human BCa tissues, we observed that LAPTM5 was significantly induced at both mRNA and protein levels, which is consistent with our microarray result. (spandidos-publications.com)

Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
It is thus likely that the previously observed phenotypes for MAK11 homologues in other eukaryotes are secondary to the main function of these proteins in ribosome formation. (pasteur.fr)

Acta Crystallogr Sect F Struct Biol Cryst Commun. (forth.gr)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (wikipedia.org)
This protein is an essential part (subunit) of a group of proteins known as the general transcription factor 2 H (TFIIH) complex. (medlineplus.gov)

This loss is accompanied with an increased expression of the histone demethylase Jmjd3 and with the recruitment of the MLL1 protein, and correlates with the expression of the Ink4a/Arf genes. (plos.org)
Inhibitors of histone deacetylase (HDAC) restore the p53 pathway in neuroblastoma cells. (nature.com)
2006) Methylation-independent binding to histone H3 and cell cycle-dependent incorporation of HP1beta into heterochromatin. (forth.gr)

Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo , but the detection of such interactions at the subcellular level remains challenging. (nature.com)
Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
Interactions of the human MCM-BP protein with MCM complex components and Dbf4. (yeastgenome.org)
The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
The interactions between these microbes can have major environmental impacts to global geochemical carbon, nitrogen and sulfur cycles. (lu.se)

Figure 8: GFP lifetime distribution of GFP:AeCRN13 and GFP:AeCRN13 AAA fusion proteins in plant nuclei. (nature.com)
In a src-transformed 3Y1 cell line, as well as other cell lines, giant cells containing polyploid nuclei with DNA contents of 16C to 32C were produced by continuous treatment with K-252a, indicating that the agent induced several rounds of the incomplete cell cycle without mitosis. (rupress.org)
A potential submicrosecond for solvent-accessible carboxyl complication in such studies is the exchange of groups to years for deeply buried amide hydrogen hydrogen nuclei between water and protein. (lu.se)

Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)

Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), DEAF-1 was identified as a candidate regulator. (sdbonline.org)

Two interrelated paradigms currently under investigation are: 1) Molecular basis of the differential expression of the gene for the small heat shock protein Alpha B-crystallin, a protein associated with with vision (e.g. in the ocular lens), oncogenesis (e.g. in breast cancer) and neural degeneration (e.g. in Alzheimer's disease). (uclahealth.org)
Hematoxylin and eosin staining was used to measure the thickness and diameter ratio of the aortic media of mice in each group, Masson staining was used to observe the content of collagen fibers in the aorta of mice in each group, elastic fibers in the aorta of mice in each group were stained, and transforming growth factor-beta and phosphoinositide 3-kinase/protein kinase B pathway protein expression. (ijpsonline.com)
It has also been demonstrated that TGF-β induced cell cycle arrest can be partially attributed to the regulatory effects of TGF-β on both the expression and activity of cyclin-dependent kinase inhibitors [CDKI] such as p21 and p27. (biomedcentral.com)
Finally, restoration of EGR1 or PTEN expression is sufficient to induce synovial sarcoma cell death. (nature.com)
1996). Expression of Egr-1 correlates with the transformed phenotype and the type of viral latency in EBV genome positive lymphoid cell lines. (nature.com)
Oncogenic PKA signaling increases c-MYC protein expression through multiple targetable mechanisms. (ucsf.edu)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
(B) Expressions of RNASET2 in human tissues were analyzed in the Human Protein Atlas database, and the resulting transcript expression values, denoted normalized expression (NX), were calculated for each gene in every sample. (frontiersin.org)
A Syntenic Cross Species Aneuploidy Genetic Screen Links RCAN1 Expression to β-Cell Mitochondrial Dysfunction in Type 2 Diabetes. (lu.se)

In an attempt to understand the function of APP, in vitro studies have focused on the identification of interacting proteins. (jneurosci.org)
Although the effective concentration of K-252a did not cause significant inhibition of affinity-purified p34cdc2 protein kinase activity in vitro, in vivo the full activation of p34cdc2 kinase during the G2/M was blocked by K-252a. (rupress.org)

The potentiation was prevented in the presence of the selective PKA inhibitor PKI 14-22 , suggesting PKA-mediated phosphorylation of the heat transducer protein. (jneurosci.org)
The forskolin-induced current potentiation was greatly reduced in cells transfected with VR-1 mutants carrying point mutations at the predicted PKA phosphorylation sites. (jneurosci.org)
PP4IP-knockout cells were generated using the CRISPR/Cas9 system, and the phosphorylation status of PP4 substrates (H2AX, KAP1, and RPA2) was analyzed. (molcells.org)

Uncoupled cell cycle without mitosis induced by a protein kinase inhibitor, K-252a. (rupress.org)

Reinitiation of DNA synthesis was inhibited by cycloheximide, actinomycin D, and serum deprivation, but not by Colcemid, suggesting that a functional G1 phase dependent on de novo synthesis of protein and RNA is essential for entry into the next S phase. (rupress.org)
Recent studies indicate that nutrient fluctuations and insulin resistance increase proinsulin synthesis in β cells beyond the capacity for folding of nascent polypeptides within the endoplasmic reticulum (ER) lumen, thereby disrupting ER homeostasis and triggering the unfolded protein response (UPR). (jci.org)

2004). Inhibition of cell growth by EGR-1 in human primary cultures from malignant glioma. (nature.com)

To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
A protein interaction landscape of breast cancer. (ucsf.edu)
All globular proteins contain large numbers of interaction, in particular, its dynamic aspects labile hydrogen atoms that can exchange with the (Daszkiewicz et al . (lu.se)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)

In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Two theories predominate: the bicellular stem cell theory and the multicellular theory. (medscape.com)
This theory holds that tumors arise from 1 of 2 undifferentiated stem cells: the excretory duct reserve cell or the intercalated duct reserve cell. (medscape.com)
Excretory stem cells give rise to squamous cell and mucoepidermoid carcinomas, while intercalated stem cells give rise to pleomorphic adenomas, oncocytomas, adenoid cystic carcinomas, adenocarcinomas, and acinic cell carcinomas. (medscape.com)
Recent evidence suggests that the bicellular stem cell theory is the more probable etiology of salivary gland neoplasms. (medscape.com)

To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)

In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
A morphological study of different stages during the reverted life cycle of Turritopsis nutricula led to the characterization of four different stages: healthy medusa, unhealthy medusa, four-leaf clover and cyst. (researchgate.net)

DNA-binding proteins (DNA-BPs) and RNA-binding proteins (RNA-BPs) have critical roles in living cells in all kingdoms of life. (nature.com)

Proteins targeted to the peroxisome (in plants called glyoxisomes) are synthesized in the cytoplasm of the cell and are targeted to the peroxisome post-translationally, possibly via multiple pathways. (tcdb.org)

Recombination between homologous sequences is a fundamentally important process both in meiosis and in mitotic cells. (brandeis.edu)

In addition, phosphorylated ERK1/2 and p38, key members of mitogen-activated protein kinase (MAPK) family regulating BCa tumorigenesis, were strongly decreased. (spandidos-publications.com)

C-terminal PTS1 [(SAC)-(KRH)-(LM)] and N-terminal PTS2 [(RK)-(LIV)-X 5 -(HQ)-(LA)] targeting sequences are recognized by Pex5p and Pex7p, respectively, which may shuttle with the substrate protein to the peroxisomal lumen. (tcdb.org)

Overexpression of DEAF-1 by using a maternal driver inhibits germ-band retraction and causes defects in dorsal closure, whereas overexpression at later stages causes cell death (Reed, 2008). (sdbonline.org)

Our main virus model is EBV, a lymphotropic herpes virus that is associated with lymphoid and epithelial cell malignancies including Burkitt's lymphoma (BL), nasopharyngeal carcinoma (NPC), immunoblastic lymphoma (IL) and Hodgkin's disease (HD). (ki.se)
We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)

Overview of all the structural information available in the PDB for UniProt: P61927 (Human 60S ribosomal protein L37) at the PDBe-KB. (wikipedia.org)
Papilloma viruses (HPV), hepatitis B- (HBV) and C-viruses (HCV), Epstein-Barr virus (EBV), Kaposi sarcoma virus (KSHV), human T-cell-leukemia virus (HTLV) may contribute to the pathogenesis of as much as 15-20% of all human cancers. (ki.se)
In the present study, we investigated FSK effects on heat-activated ionic currents in sensory neurons, as well as in human embryonic kidney 293 (HEK293) cells transfected with wild-type VR-1. (jneurosci.org)
The other project involves the study of a single nucleotide polymorphism (SNP) in the human p53 gene that results in either arginine (R) or proline (P) at position 72 of the p53 protein. (mdanderson.org)
In the next funding period we also plan to accomplish transition from mouse model to human cells and studies. (ca.gov)
Orthologous to human CDK2AP1 (cyclin dependent kinase 2 associated protein 1). (nih.gov)
This enzyme is detected in all tissues, especially in embryonic tissues and immune cells ( https://www.proteinatlas.org/ ) ( Figures 1B,C ). The full-length human RNase T2 has 256 amino acids (AA) and a predicted size of 29 kD ( Table 1 ). (frontiersin.org)
The evolutionary conservation structure of RNase T2 and distribution of RNASET2 in human tissues and immune cells. (frontiersin.org)
(C) Expressions of RNASET2 in 18 types of human blood cells and total peripheral blood mononuclear cells (PBMC) were analyzed in the Blood Atlas database. (frontiersin.org)
Daneshpajooh M, Bacos K, Bysani M, Bagge A, Ottosson Laakso E, Vikman P, Eliasson L, Mulder H, Ling C. HDAC7 is overexpressed in human diabetic islets and impairs insulin secretion in rat islets and clonal beta cells. (lu.se)

Targeted inactivation of p12, CDK2 associating protein 1, leads to early embryonic lethality. (nih.gov)

Anatomy25

Organisms16

Diseases3

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes76

Disciplines and Occupations1

Information Science3

Health Care2

Chem5

Apoptosis13

Replication24

Saccharomyces3

Proliferation14

19981

Genome Biol1

Translocation4

Complexes4

Differentiation3

20233

Golgi5

19991

Yeast3

Receptor7

Minichromosome maintenance1

Progression4

Gene encodes2

Transmembrane4

Integral membrane3

Regulation5

Receptors6

Nuclear3

Peroxisomal matrix1

Expressed in malignant1

Kinases2

Peroxisomes1

Transcriptional3

Biology2

MRNA2

Membrane protein3

Ribosome2

Commun1

Subunit2

Histone3

Interactions5

Nuclei3

Mammalian cells1

Regulator2

Expression10

Vitro2

Phosphorylation3

Kinase inhibitor1

Synthesis2

Malignant1

Interaction3

Metastasis2

Stem14

Vivo2

Characterization2

Roles1

Cytoplasm1

Homologous1

MAPK1

Lumen1

Inhibits1

Epithelial cell2

Human10

Embryonic1