• We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • The Wee1 class of protein-tyrosine kinase has an important role in cell cycle control. (berkeley.edu)
  • We investigated control mechanisms that regulate the activity, localization, and stability of Wee1, especially the bud neck-localized protein kinase Hsl1 and its more distant paralogs (Gin4 and Kcc4), in particular their recruitment to septin filaments, which assemble at the presumptive site of cell division. (berkeley.edu)
  • Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
  • Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
  • Cardiac contractility is regulated by dynamic phosphorylation of sarcomeric proteins by kinases such as cAMP-activated protein kinase A (PKA). (biomedcentral.com)
  • Efficient phosphorylation requires that PKA be anchored close to its targets by A-kinase anchoring proteins (AKAPs). (biomedcentral.com)
  • The differentiation-induced psoriasin expression was found to be mediated by the protein kinase C pathway. (medicaljournals.se)
  • The S100B interaction with calcium/calmodulin-dependent protein kinase (CaMK)-type domain of Ribosomal S6 Kinase 1 (RSK1) can facilitate therapeutic targeting of melanomas, because S100B inhibits the first phosphorylation event on RSK1 in the MAPK pathway responsible for cell growth in malignant melanomas. (elte.hu)
  • Interferon Beta Activity Is Modulated Via Binding of Specific S100 Pro" by Alexei S. Kazakov, Alexander D. Sofin et al. (usf.edu)
  • To probe selectivity of IFN-β-S100 interaction with respect to S100 proteins, we used surface plasmon resonance spectroscopy, chemical crosslinking, and crystal violet assay. (usf.edu)
  • Among the thirteen S100 proteins studied S100A1, S100A4, and S100A6 proteins exhibit strictly Ca2+-dependent binding to IFN-β with equilibrium dissociation constants, Kd, of 0.04-1.5 µM for their Ca2+-bound homodimeric forms. (usf.edu)
  • Calcium depletion abolishes the S100-IFN-β interactions. (usf.edu)
  • Interferon-α is unable of binding to the S100 proteins studied. (usf.edu)
  • The revealed direct influence of specific S100 proteins on IFN-β activity uncovers a novel regulatory role of particular S100 proteins, and opens up novel approaches to enhancement of therapeutic efficacy of IFN-β. (usf.edu)
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • S100 proteins family and their complexes with many interactions partners are mostly involved in tumors and metastasis. (elte.hu)
  • We evidenced that the equilibrium between filaments and single molecules can be controlled via S100 protein interactions and phosphorylation at the C-terminal end of the heavy chains. (elte.hu)
  • Specifically, S100 binding preferentially disassemble NM2A, while tail phosphorylation disassemble NM2B. (elte.hu)
  • Ezrin, a member of the ERM (Ezrin/Radixin/Moesin) protein family shows similar pathological properties as the S100 proteins. (elte.hu)
  • The N-terminal type A repeats in LDL receptor bind the lipoproteins. (embl.de)
  • Other homologous domains occur in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement. (embl.de)
  • Numerous familial hypercholestorolemia mutations of the LDL receptor alter the calcium coordinating residue of LDL-A domains or other crucial scaffolding residues. (embl.de)
  • The LDL receptor binds LDL and transports it into cells by acidic endocytosis. (embl.de)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Here we describe the 3.2 Å cryo-EM structure of human DEC-205, thereby illuminating the structure of the mannose receptor protein family. (uci.edu)
  • G protein-coupled receptors (GPCRs) constitute a large family of integral transmembrane receptor proteins that play a central role in signal transduction in eukaryotes. (biomedcentral.com)
  • We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)
  • In a wide variety of cell types vitamin D exerts its effects by binding to the vitamin D receptor (VDR), a member of the nuclear steroid receptor superfamily and an intracellular transcription factor [2]. (vitamindwiki.com)
  • We recently reported a novel role of discoidin domain-containing receptor 1 (DDR1) in promoting IE in breast cancer and validated its critical role in IE using neutralizing rabbit monoclonal antibodies (mAbs) in multiple mouse tumor models. (bvsalud.org)
  • This analysis revealed the presence of at least one binding domain within which visual scanning demonstrated the presence of valid binding motifs. (biomedcentral.com)
  • RBM20 binds directly to the primary RNA (pre-mRNAs) of many cardiomyopathy-associated genes, where it ensures the proper production of adult protein isoforms via an exon exclusion process (i.e., splice isoforms associated with cardiac maturation). (hilarispublisher.com)
  • CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
  • Synthetic biology requires efficient systems that support the well-coordinated co-expression of multiple genes. (bvsalud.org)
  • In this regard, we have recently shown that efficient activation of Ypk1 by TORC2 requires stimulation of TORC2 by the GTP-bound state of a Rab5 GTPase (Vps21/Ypt51) and that, in turn, Rab5-dependent endocytosis may be a mechanism to down-regulate TORC2 signaling after it has been activated. (berkeley.edu)
  • Cardiac Myosin Binding Protein-C (cMyBPC) and cardiac troponin I (cTNI) are hypertrophic cardiomyopathy (HCM)-causing sarcomeric proteins which regulate contractility in response to PKA phosphorylation. (biomedcentral.com)
  • However, since these kinases regulate a broad range of cellular responses, their compartmentalization in close proximity to their sarcomeric targets is required to facilitate control over which proteins are phosphorylated in response to second messenger signalling [ 8 , 9 ]. (biomedcentral.com)
  • Actin is a ubiquitous protein involved in the formation of filaments that are major components of the cytoskeleton. (hasenchat.info)
  • The protein is highly conserved, and forms a huge variety of structure in cells in concert with a huge numbers of actin binding proteins. (hasenchat.info)
  • Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. (hasenchat.info)
  • It has been shown that an ATPase domain of actin shares similarity with ATPase domains of hexokinase and hsp70 proteins. (hasenchat.info)
  • Further it has been suggested that members of the Rho family of small guanosine triphosphatases have emerged as key regulators of the actin cytoskeleton, and through their interaction with multiple target proteins, they ensure coordinated control of other cellular activities such as gene transcription and adhesion. (hasenchat.info)
  • Using NMR spectroscopy, we characterized the complex of S100A4 with the C-terminal actin binding domain. (elte.hu)
  • Receptors of this type are ubiquitous and transduce binding of a wide variety of extracellular ligands (peptide hormones, neurotransmitters and other bioactive compounds) into a physiological signal. (berkeley.edu)
  • We determined the binding epitope of PRTH-101 from the crystal structure of the complex between DDR1 extracellular domain (ECD) and the PRTH-101 Fab fragment with 3.15 Å resolution. (bvsalud.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • DCM can, in fact, be caused by mutations or dysregulation of multiple splicing factors [ 2 ]. (hilarispublisher.com)
  • Furthermore, RBM20 DCM is highly penetrant, linked to life-threatening ventricular arrhythmias, and manifests at a younger age than DCM caused by mutations in other proteins (e.g., laminA/C or Titin). (hilarispublisher.com)
  • Surprisingly, nonsense or missense mutations in other parts of the 1227-amino acid-long protein are uncommon, implying that they are either undiagnosed due to a mild phenotype or are not tolerated. (hilarispublisher.com)
  • Calcium binds to and regulates the small regulatory protein calmodulin that in turn binds to and regulates several hundred calmodulin binding proteins. (biomedcentral.com)
  • The Calcium Hypothesis was thus extended as the Calmodulin Hypothesis since CaM not only binds to and regulates CaMBPs critical to learning and memory but also proteins involved in the formation of amyloid plaques and tangles, hallmarks of AD [ 41 ]. (biomedcentral.com)
  • Ypk1, in turn, regulates (via its phosphorylation of multiple substrates) maintenance of sphingolipid and glycerolipid homeostasis and bilayer lipid organization in the plasma membrane. (berkeley.edu)
  • FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)
  • We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)
  • We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
  • At the same time, co-compartmentalization of enzymes or proteins that generate or terminate these second messenger metabolites, such as the phosphodiesterases (PDEs) which degrade cAMP and cGMP, with the relevant responsive kinases helps to optimise the precision and speed of response to second messenger signaling [ 10 ]. (biomedcentral.com)
  • The chelating binding mode accounts for the high affinity of this protein-carbohydrate interaction. (nature.com)
  • The LAP [leucine-rich and postsynaptic density-95/Discs large/zona occludens-1 (PDZ)] protein erbin and δ-catenin, a component of the cadherin-catenin cell adhesion complex, are highly expressed in neurons and associate through PDZ-mediated interaction, but have incompletely characterized neuronal functions. (jneurosci.org)
  • Recently, we reported a highly specific interaction between IFN-β and S100P lowering IFN-β cytotoxicity to cancer cells (Int J Biol Macromol. (usf.edu)
  • To investigate this possibility, we assessed the ability of MMGL isoform 4 to interact with PKA regulatory subunits R1A and R2A using Y2H-based direct protein-protein interaction assays. (biomedcentral.com)
  • We showed that the activation of the entire C-terminal random coil of the homodimer S100A4 protein can play a role in mediating interaction with selected partner proteins. (elte.hu)
  • A structural comparison of the predicted model with other structures of crystallized glucokinases enzymes allowed us to identify both the regions of interaction with their classical substrates (glucose and ATP) and those involved in the binding of other substrates such as fructose and ADP. (scientificarchives.com)
  • Axl2p is predicted to be a type I transmembrane protein. (embl.de)
  • The acidic residues between the fourth and sixth cysteines are important for high-affinity binding of positively charged sequences in LDLR's ligands. (embl.de)
  • S100A1/A4 proteins inhibit IFN-β-induced suppression of MCF-7 cells viability. (usf.edu)
  • The protein is present as a patch at the incipient bud site and in emerging buds, and at the bud periphery in small-budded cells. (embl.de)
  • Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • Crucially, cross-linking immunoprecipitation (CLIP) sequencing approaches have only been used to examine RBM20 RNA-binding sites in wild-type (WT) rodent CMs and HEK293 cells. (hilarispublisher.com)
  • Calcium handling and splicing defects previously observed in rodent knockout (KO) models were discovered during CM differentiation of human-induced pluripotent stem cells (iPSCs) harbouring pathological forms of RBM20. (hilarispublisher.com)
  • The presence in human red blood cells (RBCs) of a K permeability that was greatly enhanced by calcium was first reported by Gardós (1958) . (conicyt.cl)
  • Alvares K, Stern PH, Veis A. Dentin Phosphoprotein Binds Annexin 2 and is Involved in Calcium Transport in Rat kidney Ureteric Bud Cells. (chicagobiomedicalconsortium.org)
  • In LDL receptors, the class A domains form the binding site for LDL and calcium. (embl.de)
  • The pheromone receptors have seven hydrophobic segments and are coupled to a heterotrimeric G protein. (berkeley.edu)
  • In addition, δ-catenin, ARVCF, and p0071 also contain a PDZ binding motif at the C terminus. (jneurosci.org)
  • RBM20 (RNA-binding motif protein 20) is a splicing regulator found primarily in the heart and skeletal muscle, where it plays an important role in cardiac physiology. (hilarispublisher.com)
  • WT Through binding to a UCUU consensus motif in adjacent introns, RBM20 has been shown to repress exon inclusion in key regulators of cardiac excitation-contraction coupling such as TTN, CAMK2D, and RYR2. (hilarispublisher.com)
  • Accordingly, these findings suggest PPi-binding motif as potential targets for the development of inhibitors of Pi GlcK-1 activity. (scientificarchives.com)
  • The small calcium-binding protein calmodulin (CaM) is a primary effector of calcium function and works in turn by binding to and regulating CaM-binding proteins (CaMBPs) [ 53 ]. (biomedcentral.com)
  • We showed that S100A4 binds to both the N-terminal and the unstructured C-terminal ERM domain of ezrin of micromolar affinity. (elte.hu)
  • An in vitro binding kinetic analysis revealed that these phosphorylation-dependent events were attributable to a decrease in the affinity of phosphorylated Homer3 for its ligand. (jneurosci.org)
  • Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. (molcells.org)
  • A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. (molcells.org)
  • Originally thought to have only a structural role, cMyBPC has been shown to play an important role in the regulation of cardiac contractility [ 1 ], for which the N-terminal region of the protein appears to be crucial. (biomedcentral.com)
  • While loss of RBM20 expression in rodent models causes DCM, cardiac fibrosis, sudden death, and arrhythmias, few studies have focused on the precise role of mutant forms of the protein that correspond to human disease. (hilarispublisher.com)
  • MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
  • Following ingestion, lead is able to bind to and interact with many of the same enzymes as these are metals, but due to its differing chemistry, does not properly function as a co-factor, thus interfering with the enzyme's ability to catalyze its normal reaction(s). (wikidoc.org)
  • Cholecalciferol is bound to serum vitamin D-binding protein (DBP) and through a two-step enzymatic pathway involving 25-hydroxylase of the liver and 1a-hydroxylase (CYP27B1) of the kidney and extrarenal tissues, it is converted to the biologically active hormone calcitriol (1a,25(OH)2D3) [1]. (vitamindwiki.com)
  • In this sense, a better understanding of the three-dimensional (3D) structure of this protein, as well as the spatial description of the possible binding sites to various ligands would contribute to the knowledge of potential targets for the design of inhibitors of the enzymatic activity of Pi GlcK-1. (scientificarchives.com)
  • Such calculations can also be used to interpret the structures, e.g. to decide the protonation state of metal-bound ligands. (lu.se)
  • Figure 5: Structural comparison of α-DG-binding LG domains. (nature.com)
  • the bound calcium ion imparts structural integrity. (embl.de)
  • Structural information illustrated that PRTH-101 interacts with the discoidin (DS)-like domain, but not the collagen-binding DS domain of DDR1. (bvsalud.org)
  • Villalobos-Piña L, Rojas A, Acosta H. First In silico Structural Model of Glucokinase-1 from Phytophthora infestans Reveals a Possible Pyrophosphate Binding Site. (scientificarchives.com)
  • The reliability of these tools is based on the use of databases of solved protein structures that can serve as homology templates in the simulation and structural prediction of proteins [ 5 ]. (scientificarchives.com)
  • Conversely, bioinformatics tools offer an alternative that allows prediction of 3D protein structures by molecular dynamics and homology modeling making it faster, cheaper, and highly reliable. (scientificarchives.com)
  • It was soon shown that this effect depended on an increase in intracellular calcium (Cai2+) ( Whittam, 1968 ), and that the calcium sensor was located at the inside of the membrane ( Blum and Hoffman, 1972 ). (conicyt.cl)
  • As MMGL has previously been shown to interact with phosphodiesterase 4D, we speculated that it may be a PKA-anchoring protein (AKAP). (biomedcentral.com)
  • Protein structures are invaluable for biochemical studies and for mechanical or molecular mechanics calculations have become the understanding of how proteins work. (lu.se)
  • Please note that proteins can be included in multiple pathways, ie. (embl.de)
  • Guanine nucleotide-binding protein G(z) subunit alpha is a protein that in humans is encoded by the GNAZ gene. (wikipedia.org)
  • What does this gene/protein do? (cancerindex.org)
  • Homer proteins are components of postsynaptic density (PSD) and play a crucial role in coupling diverse target molecules. (jneurosci.org)
  • In animals, cadherin domain-containing proteins are adhesion molecules that modulate a wide variety of processes including cell polarization and migration but they have also been identified in yeast and magnetotactic bacteria. (embl.de)
  • δ-Catenin belongs to the p120 catenin (p120ctn) family of catenins and binds to the cytoplasmic domains of type 1 and 2 cadherins at a membrane proximal region that is not involved in binding of either β-catenin or plakoglobin. (jneurosci.org)
  • S100P is a member of large family of multifunctional Ca2+-binding proteins with cytokine-like activities. (usf.edu)
  • We show that a member of the FK506-binding protein family, FKBP8, is an essential antagonist of SHH signaling in CNS development. (silverchair.com)
  • The different mobility in the Ca2+-coordinating EF-hand sites indicate that they "communicate" by an allosteric mechanism operating through changes in protein dynamics that must be responsible for the elevated Ca2+-affinity. (elte.hu)
  • Calcium dysregulation ("Calcium Hypothesis") is an early and critical event in Alzheimer's and other neurodegenerative diseases. (biomedcentral.com)
  • At least three common themes appear to underlie all well-studied neurodegenerative diseases: disruptive protein deposits, calcium dysregulation and neuroinflammation. (biomedcentral.com)
  • Calcium dysregulation is also common to other neurodegenerative events [ 27 ]. (biomedcentral.com)
  • It is the most abundant protein in the typical eukaryotic cell, accounting for about 15% in some cell types. (hasenchat.info)
  • HACKing allows the expression level of each enzyme to be precalibrated by linking their translation to those of host proteins with predetermined abundances under the desired fermentation conditions. (bvsalud.org)
  • A single glucuronic acid-β1,3-xylose disaccharide repeat straddles a Ca 2+ ion in the LG4 domain, with oxygen atoms from both sugars replacing Ca 2+ -bound water molecules. (nature.com)
  • These proteins share common features including N-terminal (N-term) leucine-rich repeats and one to four PDZ domains, with the exception of lano that lacks a PDZ domain. (jneurosci.org)
  • Taxonomic distribution of proteins containing LDLa domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with LDLa domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing LDLa domain in the selected taxonomic class. (embl.de)
  • Percentage points are related to the number of proteins with CADG domain which could be assigned to a KEGG orthologous group, and not all proteins containing CADG domain. (embl.de)
  • Cytotoxicity and DNA cleavage with core-shell nanocomposites functionalized by a KH domain DNA binding peptide. (chicagobiomedicalconsortium.org)
  • A growing body of evidence suggests that defects in RNA splicing and protein quality control play a role in the pathogenesis of heart failure. (hilarispublisher.com)
  • Hereditary defects in membrane skeleton proteins. (dana-farber.org)
  • In this work we present the first model obtained by in silico tools of the 3D structure of Pi GlcK-1, and the binding sites to its classical substrates glucose and ATP, to fructose and ADP, and a possible binding site to PPi that is proposed as a promising target for the design of an inhibitor of this enzyme. (scientificarchives.com)
  • We also show that glycosylation of gB residue N73 may shield binding of gB-specific antibodies. (bvsalud.org)
  • However, experimental determination of protein structures remains a costly and time-consuming challenge. (scientificarchives.com)
  • The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)