• Based on the single factor test, an Box-Benhnken design was used to optimize the parameters,Lipase TLIM dosage, bath temperature, speed of stirring, for tea seed oil enzymatic hydrolysis catalyzed by lipase TLIM under probe ultrasonic emulsify, and a mathematical regression model was established. (ifoodmm.cn)
  • Under this conditions, the hydrolysis rate of tea seed oil reaches 73%, and has a good effect on tea seed oil enzymatic hydrolysis. (ifoodmm.cn)
  • Enzymatic hydrolysis of cod liver oil for the fatty acids Production [J]. Catalysis Today, 2013, 207(30): 93~100. (ifoodmm.cn)
  • Enzymes fascinate me and employing structural biology, biochemistry and bioinformatics approaches, I attempt to understand the catalytic mechanisms they employ, the rules that govern their evolution, and then engineer them for specific applications. (edu.in)
  • Enzymes used for industrial catalysis (also called biocatalysts) have to meet a great number of requirements. (lu.se)
  • Eva Nordberg Karlsson works within the fields of enzymology and microbiology, with a focus on production and biomolecular development of carbohydrate-modifying enzymes for hydrolysis (oligo- and monosaccharides) and trans-glycosylation reactions (for glycoconjugates such as surfactants and antioxidants), using the carbohydrate polymers of the biomass as the starting material. (lu.se)
  • Using a combination of bioinformatics, biochemistry and biophysics, a major thrust area in his laboratory has been to understand enzyme catalysis and unravel intriguing structure-function relationships in them with the aim of designing new anti-bacterial molecules. (edu.in)
  • Almost all the metabolic process is controlled by enzyme catalysis reaction. (priyamstudycentre.com)
  • The enzyme catalysis is highly specific in nature. (priyamstudycentre.com)
  • The efficiency of enzyme catalysis is much greater than the chemical catalyst because they lower activation energy much greater extent. (priyamstudycentre.com)
  • Our research involves a combination of molecular biology, protein biochemistry, organic synthesis and structural studies and provides a strong training for students interested in understanding the organic chemistry of living systems and in pursuing careers in biotechnology, drug design or academia. (tamu.edu)
  • Eukaryotic histone deacetylase 10 (HDAC10) is a Zn-dependent hydrolase that exhibits catalytic specificity for the hydrolysis of the polyamine -acetylspermidine. (anl.gov)
  • Our rational design based the catalytic mechanism of the α -glucosidase reaction proved to be effective for producing mutants with improved the ratio of transglycosylation/hydrolysis of α -glucosidase, which provides an important theoretical basis for further research on the reaction mechanism of α -glucosidase. (researchsquare.com)
  • Moreover, in hydrolytic retaining GH, yield of EVG is further diminished by secondary hydrolysis, a reaction in which the synthetic product becomes a substrate for the enzyme. (researchsquare.com)
  • Although increased transglycosylation/hydrolysis partition have been reported by addition of organic solvents [ 12 ], the enzyme in the reaction system never reached saturation and efficiency remained too low. (researchsquare.com)
  • It sounds like you are asking about the activation energy of the ATP hydrolysis reaction. (stackexchange.com)
  • 8] Rooneya D, Weatherley L R. The effect of reaction conditions upon lipase catalysed hydrolysis of high oleate sunflower oil in a stirred liquid-liquid reactor[J]. Process Biochemistry, 2001, 36(10): 947~953. (ifoodmm.cn)
  • N1-(5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynthesis catalyzing the hydrolysis of the N1-C6 bond of the purine substrate, a reaction unique to this pathway. (rhea-db.org)
  • Chymotrypsin also catalyses the hydrolysis of ester bonds. (nih.gov)
  • Hydrolyses: catalyze transfer of hydrolytic reactions e.g carbonsilic ester, thiolester, endoribonuclear and dipeptile hydrolyses. (researchwap.com)
  • Notably, the chemistry that accompanies the binding of ketonic substrate analogues is identical to the chemistry involved in the first step of catalysis, i.e., nucleophilic attack of a Zn-bound water molecule at the scissile carbonyl group of -acetylspermidine. (anl.gov)
  • Acylphosphatases catalyze the hydrolysis of the carboxyl-phosphate bond in acyl phosphates. (rcsb.org)
  • After correcting for the increased number of functioning active sites, determined from the pre-steady-state burst, we calculated that elevated pH also caused an increase in the velocity of the rate-limiting step, hydrolysis of the acyl-enzyme intermediate. (eurekamag.com)
  • Besides, L145I/S272T also remarkably diminished the hydrolysis activity toward the transglycosylation product EVG compared to Agl2. (researchsquare.com)
  • The calculated ΔΔG(1 → 2) values predict that the change from the A328W/Y332A mutant to the A328W/Y332G mutant should slightly improve the catalytic efficiency and that the change from the A328W/Y332G mutant to the A328W/Y332G/A199S mutant should significantly improve the catalytic efficiency of the enzyme for the (-)-cocaine hydrolysis. (uky.edu)
  • Biochemistry yielded indirect evidence for this suggestion by estimation of the binding constants of nucleotides (ATP, ADP) to catalytic and non-catalytic nucleotide sites. (mpsd.de)
  • This work highlights both structural and mechanistic similarities with the small and trimeric G proteins and restricts the types of mechanisms responsible for the considerable enhancement of ATP hydrolysis associated with actin polymerization. (rcsb.org)
  • The conservation of functionalities involved in nucleotide binding and catalysis also provide insights into the mechanistic features of members of the family of actin-related proteins. (rcsb.org)
  • Chymotrypsin -- An enzyme that catalyses the hydrolysis (the breakdown) of proteins into peptides or amino acids in the small intestine. (nih.gov)
  • The structure of myosin is known, but the actin-binding site is not well defined, and the mechanisms by which actin activates ATP hydrolysis by myosin, and myosin moves relative to the actin filament, developing force, are not fully understood. (embl.de)
  • Conserved residues in the motor domain have been positioned within the framework provided by the recent crystal structures, thus helping to define those residues involved in actin and ATP binding, in hydrolysis and in conformational change. (embl.de)
  • New insights into catalysis were gained by docking acetyl phosphate to the active site of PhAcP. (rcsb.org)
  • The terminal phosphate groups are highly reactive in the sense that their hydrolysis, or transfer to another molecule, takes place with the release of a large amount of free energy. (nih.gov)
  • Also, the binding of the Kinesin-5 inhibitors to an allosteric site on the motor interrupts the mechanism by which this enzyme converts the chemical energy of ATP hydrolysis into the mechanical work of moving microtubules, thus providing insight on how this enzyme works. (wikipedia.org)
  • The rotor (which consists of the c, ε and γ subunits) is believed to rotate relative to the stator in response to either ATP hydrolysis by F 1 or proton transport through F 0 . (tcdb.org)
  • The two active areas of the proton pump in F o and of the chemical catalysis in F 1 are apart about 10nm, but energetically coupled. (mpsd.de)
  • Insights into Enzymatic Catalysis from Binding and Hydrolysis of Diadenosine Tetraphosphate by E. coli Adenylate Kinase. (umu.se)
  • In other words, the enzyme is going through the sequence of product binding, chemical catalysis, and product release continually. (cliffsnotes.com)
  • The position of the Zn(2+)-binding site that is essential for catalysis was inferred from the positions of bound Cd(2+) ions, which were part of the crystallization medium. (rhea-db.org)
  • This allows the coupling of ATP binding and hydrolysis to electron transfer against a redox potential gradient and metal incorporation to (re)activate one of the central players of the pathway. (degruyter.com)
  • Kinetic study on hydrolysis of oils by lipase with ultrasonic emulsification[J]. Biochemical Engineering Journal, 2006, 32(1): 19~24. (ifoodmm.cn)
  • 2013). Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO fixation. (degruyter.com)
  • This is because the resting state of MoFe protein does not bind nitrogen and also requires at least three electron transfers to perform catalysis. (bionity.com)
  • The exact mechanism of catalysis is unknown due to the difficulty in obtaining crystals of nitrogen bound to nitrogenase. (bionity.com)
  • A novel computational protocol based on free energy perturbation (FEP) simulations on both the free enzyme and transition state structures has been developed and tested to predict the mutation-caused shift of the free energy change from the free enzyme to the rate-determining transition state for human butyrylcholinesterase (BChE)-catalyzed hydrolysis of (-)-cocaine. (uky.edu)
  • Research in our laboratory is funded by the German funding agency (DFG) through project grants DO-785/1 and DO-785/5 and the Cluster of Excellence 'Unifying Concepts in Catalysis-UniCat' (EXC 314). (degruyter.com)
  • Dr. Zaira Zaman Chowdhury is working as a Senior Lecturer in Nanotechnology & Catalysis Research Centre (NANOCAT), a potential National HICOE and UM COE. (um.edu.my)
  • She has completed her Post Doctoral Research Fellowship from 1/10/2013-31/03/2015 in Nanotechnology & Catalysis Research Centre (NANOCAT), University Malaya. (um.edu.my)
  • Previously, she was appointed as Research Officer in Nanotechnology & Catalysis Research Centre (NANOCAT), University Malaya from 1/04/2013 to 30/09/2013. (um.edu.my)
  • Upon receiving the award of an International Postgraduate Research Scholarship (IPRS) I joined The University of Queensland (UQ) in 1993 to carry out research towards a PhD in Biochemistry(supervisors: Assoc. Profs. (edu.au)
  • The enzyme therefore requires a great deal of chemical energy, released from the hydrolysis of ATP, and reducing agents, such as dithionite in vitro or ferredoxin in vivo . (bionity.com)