Binds operon. Medical search. Frequent questions

The substrate specificity of ABC transporters is determined by its associated substrate-binding proteins (SBPs). (nih.gov)
Chemotaxis is triggered by the binding of ligands (chemoeffectors) to chemoreceptors called methyl-accepting chemotaxis proteins (MCPs) and transducer-like proteins (Tlps). (nature.com)
An operon is a cluster of genes that are transcribed together to give a single messenger RNA (mRNA) molecule, which therefore encodes multiple proteins (Fig. 16.11). (presenternet.com)
Previous work identified two proteins in the bacterial cell (IHF, CRP) that directly affect toxin level by either directly or indirectly binding to the cellular DNA code responsible for toxin production. (usda.gov)
Stomatin/STOPP (stomatin operon partner protein) gene pairs are present in both archaeal and bacterial species, and their protein products may be involved in the quality control of membrane proteins. (rcsb.org)
The predicted structures of the Gimap proteins show common sequences and motifs, such as GTP-binding domains in the N-terminal half, but with differing C-terminal ends [ 2 , 3 ]. (hindawi.com)
In addition, cAMP binds to and regulates the function of ion channels such as the HCN channels and a few other cyclic nucleotide-binding proteins such as Epac1 and RAPGEF2 . (wikipedia.org)
Cyclic AMP binds to specific locations on the regulatory units of the protein kinase, and causes dissociation between the regulatory and catalytic subunits, thus enabling those catalytic units to phosphorylate substrate proteins. (wikipedia.org)
Protein kinase A can also phosphorylate specific proteins that bind to promoter regions of DNA, causing increases in transcription. (wikipedia.org)
RafK carries a C-terminal regulatory domain present in a subset of ATP-binding proteins that has been involved in both direct regulation of transporter activity (inducer exclusion) and transcription of transporter genes. (lu.se)
In bacteria and archaea , structural proteins with related functions are usually encoded together within the genome in a block called an operon and are transcribed together under the control of a single promoter , resulting in the formation of a polycistronic transcript ( Figure 12.30 ). (pressbooks.pub)
The observed inhibition of fibronectin binding was confirmed by a reduction in the expression of genes encoding two major fibronectin-binding streptococcal surface proteins, Sof and SfbI. (microbiologyresearch.org)
Elucidation of the hetero-dimeric binding activity of LasR and RhlR proteins with the promoter DNA and the role of a specific Phe residue during the biosynthesis of HCN synthase from opportunistic pathogen Pseudomonas aeruginosa. (bvsalud.org)
The transcription of the operon is controlled by a complex interplay between the proteins LasR and RhlR. (bvsalud.org)

Translation of the mRNA produces 4 ribosomal protein products, one of which (S4) acts as a translational repressor by binding to the nested pseudoknot region. (wikipedia.org)
This protein is found in a conserved five-gene transport operon typically found adjacent to urease genes. (nih.gov)
The Cu sensitive operon repressor (CsoR) protein determines the set point of Cu(I) concentration in the cytosol. (bl.uk)
The regulator gene codes for a repressor protein that binds to the operator, obstructing the promoter (thus, transcription) of the structural genes. (presenternet.com)
As a result, we identified a third protein (Mlc) that is required for toxin synthesis by binding to the toxin's DNA. (usda.gov)
In addition to demonstrating different interactions between CRP and Mlc and their effects on periodontal toxin production, we also discovered how the IHF protein decreases toxin production by blocking Mlc binding to the toxin DNA. (usda.gov)
Stabilization and translation of synthetic operon‐derived mRNAs in chloroplasts by sequences representing PPR protein‐binding sites. (mpg.de)
Chen X, Antson AA, Yang M, Li P, Baumann C, Dodson EJ, Dodson GG, Gollnik P (1999) Regulatory features of the trp operon and the crystal structure of the trp RNA-binding attenuation protein from Bacillus stearothermophilus . (springer.com)
In addition, the expression of these fusions are no longer cell-cycle regulated, as well as GroEL synthesis in a strain which does not have the HrcA protein, the putative repressor that binds CIRCE, indicating that the CIRCE-HrcA system are involved in cell cycle regulation of groESL in C. crescentus. (usp.br)
Slime was occasionally produced by S. caprae strains but all of them carried nucleotide sequences hybridizing at low stringency with the following genes: atlE encoding a S. epidermidis autolysin binding vitronectin and responsible for the primary adhesion to polystyrene, ica operon involved in the biosynthesis of a S. epidermidis extracellular polysaccharide, and the part of clfA encoding the serine-aspartate repeated region of a S. aureus cell-wall fibrinogen-binding protein. (unige.ch)
Import of these sugars occurs through the previously characterized raffinose ATP-binding cassette (ABC) transport system, encoded by the raf operon, that lacks the necessary ATP-binding protein. (lu.se)
In this study, we identified the raffinose ATP-binding protein RafK and showed that it was directly involved in raffinose and stachyose import. (lu.se)
The lactose utilization (lac) operon of Escherichia coli was shown to specify three enzymes and a regulatory locus, initially posited to express an RNA, but subsequently found to encode a DNA-binding repressor protein that is displaced upon lactose binding. (taylorfrancis.com)
Sublethal concentrations of manuka honey effectively prevented the binding of S. pyogenes to the human tissue protein fibronectin, but did not inhibit binding to fibrinogen. (microbiologyresearch.org)

Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)

A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. (yeastgenome.org)
Advances in molecular biology and genome analysis now also allow for detailed descriptions of DNA-binding transcription factors and transcriptional regulatory networks. (medscape.com)
This study provides the first genome-wide captures of the dynamics of in vivo AmtR binding events and the regulatory network they define. (frontiersin.org)
In this work, it was demonstrated that, in a strain overexpressing σ 32 , the levels of groESL transcripts and the synthesis of GroEL are increased, confirming that this factor is responsible for the transcriptional activation of the σ 32 -like promoter of this operon, that also presents a inverted repeat called CIRCE in its regulatory region. (usp.br)
In this context, DNA-binding transcription factors (TFs) play an important regulatory role by either repressing or activating genes in response to environmental and physiological conditions. (biomedcentral.com)
For example, the transcription, translation, and degradation of an mRNA is crucial to any gene expression event, and all three processes are controlled by a combination of elements including promoters, ribosome binding sites (RBSs), and cis -regulatory signals encoded in untranslated regions (UTRs). (universityofcalifornia.edu)
Like RafK, the presence of DLDH also activated the expression of raf operon genes, as DLDH-negative pneumococci showed a significantly decreased expression of aga and rafEFG, but DLDH did not regulate rafK or the putative regulatory genes rafR and rafS. (lu.se)
DLDH also bound directly to RafK both in vitro and in vivo, indicating the possibility that DLDH regulates raffinose transport by a direct interaction with the regulatory domain of the transporter. (lu.se)

For each of the datatypes , Pathway Tools provides querying capabilities, visualization tools (such as for drawing pathways and genome maps), and interactive editing tools to allow users to update data (such as modifying a metabolic pathway or defining a new DNA binding site for a transcription factor). (sri.com)
Ten peaks were obtained in the C. glutamicum ATCC 14067 genome including two new peaks related to three operons (RS_01910-RS_01915, RS_15995, and RS_16000). (frontiersin.org)
In this work, we identified whole-genome and metagenomic operons via MetaRon (Metagenome and whole-genome opeRon prediction pipeline). (biomedcentral.com)
MetaRon consistently achieved high operon prediction sensitivity, specificity and accuracy across E. coli whole-genome (97.8, 94.1 and 92.4%), simulated genome (93.7, 75.5 and 88.1%) and E. coli c20 (87, 91 and 88%,), respectively. (biomedcentral.com)
With MetaRon, we were able to remove two notable limitations of existing whole-genome operon prediction methods: (1) generalizability (ability to predict operons in unrelated bacterial genomes), and (2) whole-genome and metagenomic data management. (biomedcentral.com)

In collaboration with Dr. Cathy Drennan (MIT), we have determined the structure of the enzyme from E. coli with substrates bound. (hawaii.edu)
For example, in E. coli , all of the structural genes that encode enzymes needed to use lactose as an energy source lie next to each other in the lactose (or lac ) operon under the control of a single promoter, the lac promoter. (pressbooks.pub)
SQR in E. coli and B. subtilis can be assembled in the membrane without covalently bound FAD but are enzymatically inactive [3, 4, 6]. (lu.se)
The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)
The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)
It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)
The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
The lac operon of E. coli is but one example of how enzyme and transporter production can be made conditional on the presence of a nutrient to catabolize. (lu.se)
In the case of the lac operon of E. coli, a well-studied system for whether a gene is regulated. (lu.se)

In this study, we show that the dppBCDF operon of Pseudomonas aeruginosa PA14 encodes an ABC transporter responsible for the utilization of di/tripeptides. (nih.gov)
2007). Perhaps the most well-characterized polysaccharide utilization machinery in is that encoded by the operon. (acusticavisual.net)

The alpha operon ribosome binding site in bacteria is surrounded by this complex pseudoknotted RNA structure. (wikipedia.org)
into the cytoplasm where they can exert their bactericidal effect which requires binding to the ribosome ( Davis, 1987 ). (elifesciences.org)

The promoters for nimR and the nik operon are divergent and overlapping and NimR binds at a site between the promoter elements for nikKLMQO . (rsc.org)

In S. lividans the GlxA gene is part of the cslA/glxA operon that contains genes encoding putative enzymes involved in glycan processing. (bl.uk)
A typical operon consists of a group of structural genes that code for enzymes involved in a metabolic pathway, such as the biosynthesis of an amino acid. (presenternet.com)
What enzymes are produced by the operon? (presenternet.com)
Three of the enzymes for lactose metabolism are grouped in the lac operon: lacZ, lacY, and lacA (Figure 12.1. (presenternet.com)

This was not helpful since it included a huge number of sites and sequences that result from spurious (nonfunctional) binding of transcription factors or accidental transcription of random DNA sequences to make junk RNA [see What did the ENCODE Consortium say in 2012? ]. (blogspot.com)

Chapter 4 addresses another surprising finding in that a putative haem peroxidase (DtpA), part of the sco operon, plays a role in GlxA maturation and in the Cu-dependent morphological development. (bl.uk)

Binding of ferrous iron to the DtxR molecule forms a complex that binds to the tox gene operator and inhibits transcription. (medscape.com)
or a repressor may not be able to bind to the operator unless it is bound to another molecule, the corepressor. (presenternet.com)

Genes are transcriptional units, and include bacterial operons. (yeastgenome.org)
Under Cu stress, Cu(I) binds to CsoR and de-represses genes under its transcriptional control. (bl.uk)

Operons aiding microbial survival are crucial in understanding the gene regulation, identification of new pathways and novel products in diverse environmental settings. (biomedcentral.com)

From our work , we could predict the importance of a specific amino acid residue Phe214 from RhlR which might be considered to have the desired specificity to bind to the promoter DNA . (bvsalud.org)

Expression of this operon requires NimR and depends on Ni 2+ . (rsc.org)
The expression of the groESL operon is induced by heat shock and is cell cycle controlled at normal temperatures, with maximal transcription in the predivisional cell and very low levels in the stalked cell. (usp.br)
Pneumococci lacking RafK showed a 50- to 80-fold reduction in expression of the raf operon genes aga (alpha-galactosidase) and rafEFG (raffinose substrate binding and permease genes), and both glucose and sucrose inhibited raffinose uptake through inducer exclusion. (lu.se)
Explain how the presence of glucose will affect expression of the ara operon. (wikiversity.org)

A functional model is presented in which IHF favors the contact between the TodT activator, bound further upstream, and the alpha-subunit of RNA polymerase bound to the downstream promoter element. (nih.gov)

What is an operon enzyme? (presenternet.com)
On the basis of this structure, and related mechanistic studies, we have proposed that the biotin thiophane sulfur is derived from a [2Fe-2S] cluster bound within the core of the enzyme. (hawaii.edu)
The covalent bond to FAD in SQR is essential for enzyme activity and the main reason for this is that the bond raises the midpoint redox potential of the flavin by more than 80 mV compared to non-covalently bound flavin. (lu.se)

In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

Efficient regulation of bacterial genes in response to the environmental stimulus results in unique gene clusters known as operons. (biomedcentral.com)

Structural genes - the genes that are co-regulated by the operon. (presenternet.com)

The interactions between AmtR and the promoter regions of the three operons were confirmed by electrophoretic mobility shift assays (EMSAs). (frontiersin.org)

Here, the function of regulation is quite clear: expressing energy and carbon, that a number around 0.2% would be the right genes at the right time will enable the cell to make the expected, and that the difference is more or less specific to the lac most of the resources within its reach, by maximizing the uptake operon [7]. (lu.se)

We also demonstrate the use of operons as a subset to represent the trends of secondary metabolites in whole-metagenome data and the role of secondary metabolites in the occurrence of disease condition. (biomedcentral.com)

Unless an inducer is present, activators don't bind well to activator DNA. (presenternet.com)

This page displays every known transcription unit of this operon and their known regulation. (unam.mx)

SQR consists of a membrane-peripheral heterodimer domain (often called succinate dehydrogenase) which is tightly bound to a membrane-spanning anchor, which is a cytochrome with one or two heme groups depending on the organism. (lu.se)

Toluene binds to TodS with high affinity (Kd = 684 +/- 13 nM) and 1:1 stoichiometry. (nih.gov)

The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)

Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
Fqo can be hypothesized to be operative during sulfate reduction with CO. The Fqo complex receives electrons from the reduced coenzyme F 420 (F 420 H 2 ), generated from the oxidative acetyl-CoA pathway, and transfers electrons to the membrane-bound respiratory chain by the reduction of menaquinone [ 16 - 18 ]. (hindawi.com)

Flavinylation of Fp in B. subtilis does not require the Ip subunit or the membrane anchor but depends on folding of the Fp polypeptide into a state that can bind FAD and subsequent mediate covalent binding of the FAD [3, 4]. (lu.se)

Till date, there is no report that deals with the binding interactions of the RhlR-LasR heterodimer with the promoter DNA region of the hcnABC operon . (bvsalud.org)
We, for the first time , tried to analyse the binding modes of the RhlR-LasR heterodimer with the promoter DNA regions. (bvsalud.org)

Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)

Integration host factor (IHF) plays a crucial role in the activation process and binds between the upstream TodT boxes and the -10 hexamer region. (nih.gov)

Notably, certain B. subtilis mutants with mutated Fp lack covalently bound flavin and do not assemble the SQR polypeptides in the membrane [3]. (lu.se)

The complex binding to the activation sequence activated the target genes. (presenternet.com)

TodS transphosphorylates TodT, which binds to two highly similar DNA binding sites at base pairs -107 and -85 of the promoter. (nih.gov)
What effect, if any would be the effect of deleting the araO 2 sites where AraC binds? (wikiversity.org)

Chapter 5 structurally characterises SliLPMO10E and also investigates the kinetics of Cu-binding. (bl.uk)

The toxin is a single polypeptide with an active (A) domain, a binding (B) domain, and a hydrophobic segment known as the T domain, which helps release the active part of the polypeptide into the cytoplasm. (medscape.com)
The analysis of the truncated variants of TodS reveals that toluene binds to the N-terminal input domain (Kd = 2.3 +/- 0.1 microM) but not to the C-terminal half. (nih.gov)
The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)

The structure of 1510-C had a compact five-stranded β-barrel fold known as an oligosaccharide/oligonucleotide-binding fold (OB-fold). (rcsb.org)

Operator - a segment of DNA to which a repressor binds. (presenternet.com)

The latter brings to the attention that LPMOs are able to bind Cu in two forms at a single site before relaxing into a final substrate active form. (bl.uk)

In the presence of ferrous iron, the DtxR-iron complex attaches to the tox gene operon, inhibiting transcription. (medscape.com)

Transcription of the ltxA operon is induced during anaerobic growth. (usda.gov)

Anatomy2

Organisms5

Chemicals and Drugs29

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes40

Information Science3

Proteins14

Protein14

Escherichia1

Regulatory8

Genome5

Coli10

Utilization2

Ribosome2

Promoters1

Enzymes4

Sequences1

Putative1

Molecule2

Transcriptional2

Microbial1

Specificity1

Expression4

Polymerase1

Enzyme3

19871

Bacterial1

Structural1

Promoter regions1

Uptake1

Subset1

Inducer1

Regulation1

Consists1

High-affinity1

Clusters1

Membrane-bound2

Depends1

Heterodimer2

Genetic1

Upstream1

Lack1

Sequence1

Sites2

Kinetics1

Domain3

Fold1

Operator1

Single1

Complex1

Growth1