• Reference: Selective oxidation of the exonuclease domain of bacteriophage T7 DNA polymerase. (neb.com)
  • Bacteriophage T7 DNA polymerase, the product of gene 5 of the phage, has both polymerase and single-and double-stranded DNA 3'-to 5'-exonuclease activities. (neb.com)
  • Gp5 (encoded by gene gp5) is T7 phage's DNA polymerase. (wikipedia.org)
  • T7 polymerase uses E. coli's endogenous thioredoxin, a REDOX protein, as a sliding DNA clamp during phage DNA replication (though thioredoxin normally has a different function). (wikipedia.org)
  • T7 DNA polymerase, assisted by E. coli thioredoxin, performs both leading and lagging-strand DNA synthesis. (wikipedia.org)
  • This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. (justia.com)
  • Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. (justia.com)
  • T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. (justia.com)
  • 1. An autogene, comprising a gene encoding a T7-like bacteriophage RNA polymerase under the control of an autogene promoter recognized by said polymerase. (justia.com)
  • b) a naturally occurring bacteriophage promoter having an alteration which changes the strength of the promoter relative to that of the wild type promoter, but is still recognized by said polymerase. (justia.com)
  • 9. The autogene of claim 8, wherein the autogene promoter is recognized by T7 RNA polymerase and the lac operator is centered at a position from +12 to +21 relative to the transcriptional start site. (justia.com)
  • Rifampicin can be used in the inhibition of bacteriophage T7 RNA polymerase. (p212121.com)
  • Using the hexameric helicase and the DNA polymerase from bacteriophage T7 as model systems, we provide stepwise procedures for measuring the kinetics of the reactions they catalyze based on radioactivity and fluorescence. (elsevierpure.com)
  • T7 RNA Polymerase ( T7 RNAP, EC 2.7.7.6) is a DNA-directed RNA polymerase cloned from bacteriophage T7 and overexpressed in yeast H. polymorpha . (artes-biotechnology.biz)
  • E. coli is more resistant to T7 than to some other similar phages. (wikipedia.org)
  • Formerly, the phylogenetic and taxonomic classification of bacteriophages (phages) was based on similarities in phage morphology, host range [ 1 ], ability to recombine, and also on similar cross-hybridization patterns of their DNAs [ 2 - 4 ]. (biomedcentral.com)
  • In the last few years, a large number of T7-like phages have been sequenced most of which have been gathered in a new viral subfamily within the family Podoviridae . (biomedcentral.com)
  • On the basis of TEM micrographs this virus resembled T7-like phages with a head diameter of about 63 nm and a noncontractile tail size approximately 13 × 8 nm. (biomedcentral.com)
  • The size of this phage correlates well with other T7-like phage members, where the smallest and largest phages reported so far are the Pseudomonas putida phage gh-1 (37.4 kb) and the Vibrio parahaemolyticus phage VpV262 (45.9 kb), respectively [ 12 ]. (biomedcentral.com)
  • These are called bacteriophages, or simply phages. (lu.se)
  • Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. (wikipedia.org)
  • Before being physically referred to as T7, the phage was used in prior experiments. (wikipedia.org)
  • The genome of phage T7 was among the first completely sequenced genomes and was published in 1983. (wikipedia.org)
  • T7 has a life cycle of 17 min at 37˚C, i.e. the time from infection to the lysis of the host cell when new phage are released. (wikipedia.org)
  • The T7 phage recognizes certain receptors on the surface of E.coli cells, and binds to the cell surface by its viral tail fibers. (wikipedia.org)
  • The short, stubby tail of the T7-like phage is too short to span the cell envelope and, in order to eject the phage genome into the cell at the initiation of infection, virion proteins must first make a channel from the tip of the tail into the cell cytoplasm. (wikipedia.org)
  • Once the T7 phage has inserted the viral genome, the process of DNA replication of the host genome is halted and replication of viral genome begins. (wikipedia.org)
  • citation needed] Under optimal conditions, the T7 phage can complete the lytic process within 25 minutes, leading to the death of the E. coli host cell. (wikipedia.org)
  • Phage T7 has the simplest known DNA replisome, consisting of a helicase and primase that reside in a single polypeptide chain that forms a hexamer in the presence of DNA and ATP or dTTP. (wikipedia.org)
  • In phage T7, DNA double-strand breaks are likely repaired by insertion of a patch of donor DNA into a gap at the break site. (wikipedia.org)
  • Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects susceptible bacterial cells, that is composed of DNA and infects most strains of Escherichia coli. (shapeways.com)
  • Bacteriophage T7 has a lytic life cycle and several properties that make it an ideal phage for experimentation. (shapeways.com)
  • Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. (biomedcentral.com)
  • Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1. (biomedcentral.com)
  • That this phage is closely related to Pseudomonas putida phage gh-1 and coliphage T7 places it in the "T7-like viruses" genus of the subfamily Autographivirinae within the family Podoviridae . (biomedcentral.com)
  • In the 1940s, Max Delbruck encouraged the phage group at Cold Spring Harbor to concentrate their research on seven specific bacteriophages (T1 -- T7), so that they could readily compare results. (nih.gov)
  • T7 grows on rough strains of Escherichia coli (i.e. those without full-length O-antigen polysaccharide on their surface) and some other enteric bacteria, but close relatives also infect smooth and even capsulated strains. (wikipedia.org)
  • The 63 kDa gene 4 protein of bacteriophage T7 provides both helicase and primase activities. (elsevierpure.com)
  • The only bacteriophages known to have a lipid envelope around their protein capsids are the members of the Cystoviridae family [ 6 ]. (biomedcentral.com)
  • The researchers concluded that protein inhibitors encoded by bacteriophage genes are a newer line in the evolutionary arms race between bacteria and viruses. (outbreaknewstoday.com)
  • It is a fantastic recognition of the work that people in our lab have been doing for many years: first on protein synthesis, antibiotics targeting it and antibiotic resistance mechanisms that counter the antibiotics - and more recently, on bacterial viruses, bacteriophages. (lu.se)
  • This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls (preferentially: D-lactyl-L-Ala). The structure is known for the bacteriophage T7 structure and shows that two of the conserved histidines are zinc binding. (nih.gov)
  • c) a naturally occurring T7 promoter having an alteration in the -17 to +6 region. (justia.com)
  • 6. The autogene of claim 5, wherein the autogene promoter is the T7.phi.10 promoter whose DNA sequence differs from natural T7 class III promoters by containing AG instead of GA at positions +4 and +5, where +1 is the transcriptional start site. (justia.com)
  • Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. (justia.com)
  • Using high-throughput sequencing of genomic DNA of bacterial mutants resistant to common antibiotics and bacteriophage inhibitors, the researchers identified two previously known inhibitors as well as a novel inhibitor ('gene product 0.6') that prevented bacterial growth and eventually led to cell lysis. (outbreaknewstoday.com)
  • On the heels of President Obama's announcement to increase spending on antibiotic resistance to $1.2B , researchers from Tel Aviv University (TAU) have shown that proteins embedded in bacteria by bacteriophages could lead to a new approach to fight drug resistant microbes. (outbreaknewstoday.com)
  • Bacteriophages are viruses that only infect bacteria, and are therefore one of the most common entities in the biosphere. (outbreaknewstoday.com)
  • Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects. (wikipedia.org)
  • I got my ScD in Biological Engineering at MIT in Drew Endy 's lab and worked on understanding the development of a simple virus (T7) that infects E. coli . (openwetware.org)
  • Such a feature is unique among bacterial viruses (i.e., bacteriophages) and the mechanisms of virion envelopment within a bacterial host are largely unknown. (biomedcentral.com)
  • In addition, there are 25 nonessential proteins, of which only 12 are conserved across the genus "T7-like viruses" [ 8 ]. (biomedcentral.com)
  • Compared to the genome of gh-1, the sequence of ϕIBB-PF7A is longer and contains more genes with unassigned function and lacks a few potentially essential and non-essential T7 genes, such as gene1.1, 3.8, and 7. (biomedcentral.com)
  • The genome of coliphage T7 has 56 genes encoding potential proteins [ 8 ], of which 35 have known function or functions. (biomedcentral.com)
  • This virus is the first T7-like bacteriophage for P. fluorescens for which the genome has been determined. (biomedcentral.com)
  • The amount of enzyme required to hydrolyse 4 nmol of NTP per hour at 37 °C in an in-vitro transcription reaction using 1 µg of T7 template encoding firefly luciferase. (artes-biotechnology.biz)
  • Because of their selectivity, bacteriophages are being tested for use in treating human bacterial diseases such as Inflammatory Bowel Disease and many infections , albeit with limited success. (outbreaknewstoday.com)
  • T7 bacteriophage has been evolved to override several of the host bacteria's defenses including the peptidoglycan cell wall and the CRISPR system. (wikipedia.org)
  • The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance. (nih.gov)
  • In some strains of T7, the tail fibers are replaced with tail-spikes that degrade the O- or K-antigens on the cell surface by way of enzymatic activity. (wikipedia.org)
  • A significant dependence of opening probability and waiting time between bubble events on the local DNA sequence is revealed and quantified for a biological sequence of the T7 bacteriophage. (bgu.ac.il)
  • However, high-fitness strains of T7 have been isolated with a latent period of only ~11 min at 37˚C growing under optimal conditions in rich media results. (wikipedia.org)
  • However, until the recent identification of abortive RNA in the bacteriophage T7 with transcriptional antitermination function, they had been considered only as "junk" RNAs. (confex.com)
  • The bacteriophage molecule, but not its moth homologue, has been shown to have N-acetylmuramoyl-L-alanine amidase activity. (embl-heidelberg.de)
  • dgt gene, Dgt) that establishes the normal dGTP level required for accurate DNA replication but also plays a role in protecting E. coli against bacteriophage T7 infection by limiting the dGTP required for viral DNA replication. (nih.gov)
  • It was used to monitor T7 interaction with E. coli . (biofisica.info)
  • The device was able to determine the viability of a low amount of living bacteria attached to the cantilever, and was used to monitor T7 interaction with E. coli over a wide range of virus concentrations up to 109 PFU ml-1. (biofisica.info)
  • 37. An unstructured mRNA region and a 5' hairpin represent important elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site. (nih.gov)
  • Four proteins of known crystal structure are sufficient for the basis reactions: T7 DNA polymerase and its processivity factor (E. coli thioredoxin), T7 helicase-primase, and T7 ssDNA binding protein. (nih.gov)
  • Upon joining the faculty at Harvard Medical School the Richardson laboratory discovered T4 polynucleotide kinase, T4 DNA ligase, T7 DNA polymerase, helicase, and primase, and E. coli exonuclease VII and DNA polymerase II. (nih.gov)
  • Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE , that infects E. coli. (nih.gov)
  • Bacteriófago virulento que es especie tipo del género de fagos similares a T7, de la familia PODOVIRIDAE, que infecta a E. coli. (bvsalud.org)
  • 29. In vivo and in vitro activities of point mutants of the bacteriophage T7 RNA polymerase promoter. (nih.gov)
  • There he told me a story about how the bacteriophage T7 polymerase/promoter system was developed. (virology.ws)
  • Reverse-transcribed sub-genomic complementary DNA (cDNA) fragments were enzymatically amplified using generic oligonucleotide primers and were assembled as a complete infectious genomic copy (pCVB3-0) downstream of the T7 RNA polymerase promoter. (nebraska.edu)
  • The economy of proteins found in the replication system of bacteriophage T7 has made possible the analysis of a replisome at the molecular level. (nih.gov)
  • In recent years the Richardson laboratory has focused on the replication system of bacteriophage T7. (nih.gov)
  • Replication of genomic DNA by T7 DNA polymerase, formation of viral genome concatemers. (expasy.org)
  • Genomic DNA from bacteriophage T7 was co-eletroporated into cells, along with synthetically produced oligonucleotides that encode a desired genetic change. (nih.gov)
  • Excess information at bacteriophage T7 genomic promoters detected by a random cloning technique. (fred.net)
  • 27. Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection. (nih.gov)
  • 7. Hu B, Margolin W, Molineux IJ, Liu J, Structural remodeling of bacteriophage T4 and host membranes during infection initiation, Proc Natl Acad Sci U S A,112(35):E4919-28, 2015. (cryoet.org)
  • 2. Wang C, Tu J, Liu J, Molineux IJ: Structural Dynamics of Bacteriophage P22 Infection Initiation Revealed by Cryo-electron Tomography,Nature Microbiology,2019. (cryoet.org)
  • Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. (lookformedical.com)
  • 30. Two base pairs at -9 and -8 distinguish between the bacteriophage T7 and SP6 promoters. (nih.gov)
  • 26. Mechanism of toxicity of 3-methyladenine for bacteriophage T7. (nih.gov)
  • 39. Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase. (nih.gov)
  • PROCEEDINGS NATIONAL ACADEMY OF SCIENCES REACTIVATION OF IRRADIATED BACTERIOPHAGE BY TRANSFER OF SELF-REPRODUCING UNITS By S. E. Luria DEPARTMENT OF BACTERIOLOGY, INDIANA UNIVERSITY, BLOOMINGTON, INDIANA Communicated June 13, 1947 In investigating certain peculiarities in the titration of the activity of bacteriophages after ultraviolet irradiation, a mechanism was discovered ' that offers direct support for interpreting inactivation of these viruses by tadiation as due to lethal mutations. (nih.gov)
  • 21. The T7 concatemer junction sequence interferes with expression from a downstream T7 promoter in vivo. (nih.gov)
  • 31. Inhibition of T7 RNA polymerase by T7 lysozyme in vitro. (nih.gov)
  • 36. Expression of genes 51, 27, 28 coding for proteins of the central part of bacteriophage T4 baseplate in the bacteriophage T7 promoter/RNA polymerase expression system. (nih.gov)
  • 32. Tests of a model for promoter recognition by T7 RNA polymerase: thymine methyl group contacts. (nih.gov)
  • 28. Real time monitoring of the interaction of T7 RNA polymerase with azobenzene-tethered T7 promoter by biosensor. (nih.gov)
  • 33. Studies on the interaction of T7 RNA polymerase with a DNA template containing a site-specifically placed psoralen cross-link. (nih.gov)
  • V , Krasilnikova, MM & Kochetkov, SN 1990, ' Studies of the nucleoside triphosphate binding site of bacteriophage T7 DNA-dependent RNA-polymerase using GTP analogs ', Biokhimiya , vol. 55, no. 5, pp. 829-835. (psu.edu)
  • We have developed a system for rapidly and efficiently modifying bacteriophages for use as therapeutic agents. (nih.gov)
  • Results for Reference: Two forms of the DNA polymerase of bacteriophage T7. (neb.com)
  • The UV stress on microorganisms and/or molecules essential for life was estimated by using DNA damaging effects (specifically bacteriophage T7 killing and uracil dimerization) for various irradiation conditions on the present and ancient Martian surface. (bioone.org)
  • The method uses the simultaneous enzymatic activities of avian RT, ribonuclease H and bacteriophage T7 RNA polymerase. (medscape.com)
  • The following product was used in this experiment: T7 Tag Polyclonal Antibody, HRP from Thermo Fisher Scientific, catalog # A190-116P. (thermofisher.com)