Bacteria luminescent proteins. Medical search. Frequent questions

Biologist David Gruber studies radiant creatures and their fluorescent proteins. (the-scientist.com)
He is purifying its fluorescent proteins and hopes they can someday be used as luminescent tags in biomedical research. (the-scientist.com)
Few scientists knew the complete history of fluorescent protein research, so he and Pieribone wrote a book about it. (the-scientist.com)
After publishing Aglow in the Dark in 2005, Gruber moved to Brown University for postdoctoral study on ways to use fluorescent proteins as biological sensors. (the-scientist.com)
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. (bvsalud.org)
Our results provide a structural basis for understanding the red fluorophore formation mechanism and demonstrate that protein engineering of GFPs is a promising way to create RFPs suitable for fluorescent tags. (bvsalud.org)
Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically encodable bioprobes and biomarkers that can advance next-generation breakthroughs across the imaging and life sciences. (bvsalud.org)
In specific aspects, the screenable marker is a fluorescent or luminescent polypeptide. (justia.com)
COATING PROCESSES : ELECTRICAL PRODUCT PRODUCED : Fluorescent or phosphorescent base coating (e.g., cathode-ray tube, luminescent screen, etc. (everypatent.com)
Particular importance here is the biological applications of Pt NCs such as the bioimaging of various cells as a preferred fluorophore in contrast to traditional fluorescent markers (e.g., organic dye, semiconductor quantum dots, and fluorescent proteins), the usage of Pt NCs-based antitumour drugs as a new class chemotherapeutics for malignant tumour therapy, and the utilization of antibacteria as an alternative of Ag-based antibacterial agent. (hindawi.com)

Culture methods often involve measuring the diameter of areas without bacterial growth, called zones of inhibition, around paper discs containing antibiotics on agar culture dishes that have been evenly inoculated with bacteria. (wikipedia.org)
The disc diffusion method involves selecting a strain of bacteria, placing it on an agar plate, and observing bacterial growth near antibiotic-impregnated discs. (wikipedia.org)
This altered gene expression also correlated directly with protein levels, bacterial capacity for DNA uptake, and natural transformability. (biomedcentral.com)
Natural competence is a mode of horizontal gene transfer, which is based on the ability of a bacterium to take up free DNA from the environment and recombine it with the bacterial genome resulting in natural transformation. (biomedcentral.com)
In other particular aspects, the bacterial cell is an Enterobacter or Serratia bacteria. (justia.com)
The genes for the bacterial, firefly, and jellyfish luminescent proteins can be spliced into the genes of other organisms to monitor gene activity. (ucsd.edu)
Often starting as a phagosome in the endocytic compartment, these bacteria-occupied vacuoles transition into organelles with novel molecular features via remodeling of host proteins and lipids through the highly coordinated actions of bacterial factors [3] [5] . (microbialcell.com)

Photoproteins, luminescent proteins or optoproteins are a kind of light-response protein responsible for the conversion of light into biochemical energy that is used by some bacteria or fungi to regulate specific biological processes. (bvsalud.org)
The classification of these optoproteins from bacteria and fungi as photoreceptors or photoresponse elements according to the excitation-emission spectrum (UV-Vis-IR), as well as their potential use in novel applications, is addressed in this article by providing a structured scheme for this broad area of knowledge. (bvsalud.org)
The most extensive agricultural losses are attributable to weed invasion, animal pests, and also plant diseases caused by bacteria, fungi, and viruses. (thecbdtips.com)

Live flashlight fish, filled with bioluminescent bacteria, inhabit the exhibit as well, blinking what looks like a secret code to passers-by. (the-scientist.com)

Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. (addgene.org)
Bioluminescence occurs when the protein luciferin is oxidized by the enzyme luciferase in the presence of ATP and oxygen. (kenyon.edu)
The chance that P. fusiformis' predator will be seen and eaten as a result of bioluminescence is greater than the chance that the entire P. fusiformis colony will be preyed upon, which favors the survival of the P. fusiformis group as well as the luminescent genotype. (kenyon.edu)
Luminous scans, also known as luminescent imaging or bioluminescence imaging, involve the use of light-emitting molecules to visualize and track biological processes within living organisms. (rantdriven.com)

Sensitivity testing usually occurs in a medical laboratory, and uses culture methods that expose bacteria to antibiotics, or genetic methods that test to see if bacteria have genes that confer resistance. (wikipedia.org)

This is because Escherichia coli is the most likely causative bacterium, and may be sensitive to that combination antibiotic. (wikipedia.org)
BioSPLICE involves a recombinant plasmid vector containing a DNA aptamer insert (DNA Capture Element) in the host bacterium species/strain Escherichia coli JM109 or HB101, or a mammalian cell. (conceptualmindworks.com)
Pathogen reduction by predatory bacteria and survival of Bdellovibrio bacteriovorus and Escherichia coli on produce and buffer treated with low-dose gamma radiation. (usda.gov)

Previous studies of beta-cells focused on protein kinase A (PKA) as a downstream effector of cAMP action. (nih.gov)
Researchers can introduce these glowing proteins into cells to track all kinds of activity, including the growth of tumors or chatter between nerve cells. (the-scientist.com)
We also show that a monomeric variant of AzamiRed1.0 can be used for labeling organelles and proteins in mammalian cells. (bvsalud.org)
These are the infamous "spike proteins" that are being coded into human cells using the Covid-19 "vaccination" program. (truthmed.social)
The coding of human cells with chimeric "spike proteins" through Reverse Genetics , is done by electroporation using programmable Gold Nanobots and CRISPR-Cas9. (truthmed.social)
Cancer cells, like bacteria, can develop resistance to drug therapy. (news-medical.net)
One membrane component that is often subverted by vacuolar bacteria is cholesterol - an abundant lipid that mammalian cells produce de novo at the endoplasmic reticulum (ER) or acquire exogenously from serum-derived lipoprotein carriers. (microbialcell.com)
Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. (invivogen.com)
Additionally, such designer made nanomaterials has potential to generate series of interactions with various biological entities including DNA, proteins, membranes, cells and organelles at nano-bio interface. (springeropen.com)

pMWNTs coated with autologous serum proteins prior to injection accumulated almost entirely in the lung and liver in large dark clusters. (cnrs.fr)

By engineering bacteria or viruses to produce light-emitting molecules, scientists can visualize the spread of these pathogens within a living organism. (rantdriven.com)

OMV produced by bacteria, genetically modified to increase their spontaneous release, simplifying their production, are also known as Generalized Modules for Membrane Antigens (GMMA). (mdpi.com)
Hydras and parasites are being first transfected with chimeric pathogens using Plasmid DNA (E. coli bacteria and fungus) to produce a new genetically modified line of Hydras. (truthmed.social)

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
This protein kinase is calcium-independent and phospholipid-dependent. (nih.gov)

In a recent case study, luminous scans were used to screen a library of compounds for their ability to inhibit a specific protein involved in a neurodegenerative disease. (rantdriven.com)

Burkholderia thailandensis, an opportunistic pathogen found in the environment, is a bacterium closely related to B. pseudomallei, the cause of melioidosis. (cdc.gov)

Abcam: antibodies, proteins, kits. (abcam.com)
The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies. (jove.com)

Organizations such as the American Type Culture Collection and National Collection of Type Cultures provide strains of bacteria with known resistance phenotypes that can be used for quality control. (wikipedia.org)

ARLINGTON, Virginia - Israel-based company Enzymit and the Environmental Microbiology and Biosensor Laboratory at the Hebrew University of Jerusalem have introduced a new biosensor that uses the bacterium E. coli to detect unexploded ordnance. (nationaldefensemagazine.org)
A bacterium like E. coli is "essentially a very simple cell," with built-in protein sensors to perform its natural functions, Enzymit CEO Gideon Lapidoth said in an interview. (nationaldefensemagazine.org)
E. coli already has a sensor to detect TNT, which it can metabolize and use as fuel, but the university lab was able to change the nature of the bacterium "so instead of having the bacteria just break down TNT, it activates a luminescent protein" that was derived from a different organism, he said. (nationaldefensemagazine.org)
Additionally, "the genetically engineered properties of E. coli cause the bacterium to die out shortly after deployment, ensuring it does not pose any human or environmental risk," an Enzymit release said. (nationaldefensemagazine.org)
After sufficient incubation time for the E. coli to take up the plasmid-coated nanoparticles (uncoated nanoparticles will also be taken up), the bacteria are plated on solid media (agar) or on a media impregnated filter containing ampicillin alone or LB agar plates with 100µg/ml ampicillin. (conceptualmindworks.com)

Testing based on exposing bacteria to antibiotics uses agar plates or dilution in agar or broth. (wikipedia.org)
To ensure that the results are accurate, the concentration of bacteria that is added to the agar or broth (the inoculum) must be standardized. (wikipedia.org)
When you receive the plastic vial of stab culture, please streak the bacteria on an LB agar plate containing 12.5 ug/mL chloramphenicol. (riken.jp)
When you streak bacteria, please take bacteria from the middle part of stab agar medium but not those from the surface of the stab which are not really healthy. (riken.jp)

Both Firefly luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. (addgene.org)
Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. (addgene.org)

This reporter strain responds to agents that damage DNA (with an apparent detection limit of 1 µg of mitomycin C) without observable response to membrane integrity damage, protein folding or oxidative stress generating agents, in the latter case, DNA damage was observed. (springeropen.com)

The conceptualization of aggregation-induced emission (AIE) breaks this common belief and provides a new stage for the exploration of practically useful luminescent materials for optical, electronic, energy and biomedical applications. (european-mrs.com)

The associated bacteria not only safeguard plants but also kill this fungus using enzymes that degrade chitin (right). (thecbdtips.com)
Using beef tomato plants as an example, Heermann's team of microbiologists showed that infestation by the phytopathogenic fungus Fusarium graminearum can be prevented by the bacteria as they colonize the fungal hyphae, breaking down the chitin there. (thecbdtips.com)
This enables the bacteria to dissolve the structure of a fungus, specifically its cell wall, and effectively inhibit fungal growth. (thecbdtips.com)

This plasmid can be extracted and bound specifically to a molecular target (i.e. protein, polysaccharide, lipid, or nucleic acid) immobilized covalently or non-covalently on a paramagnetic metallic nanoparticle. (conceptualmindworks.com)

Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. (wikipedia.org)
Sensitivity testing results can allow a clinician to change the choice of antibiotics from empiric therapy, which is when an antibiotic is selected based on clinical suspicion about the site of an infection and common causative bacteria, to directed therapy, in which the choice of antibiotic is based on knowledge of the organism and its sensitivities. (wikipedia.org)
The minimum inhibitory concentration, which is the lowest concentration of the antibiotic that stops the growth of bacteria, can be estimated from the size of the zone of inhibition. (wikipedia.org)
This method of antibiotic selection is called empiric therapy, and it is based on knowledge about what bacteria cause an infection, and to what antibiotics bacteria may be sensitive or resistant. (wikipedia.org)
or quantitative, using a minimum inhibitory concentration (MIC) to describe the concentration of antibiotic to which a bacterium is sensitive. (wikipedia.org)
Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. (invivogen.com)

Additionally, there are bioluminescent proteins that produce light during a heatless chemical reaction and are useful to be employed as biomarkers in several fields such as imaging, cell biology, disease tracking and pollutant detection. (bvsalud.org)
bacteria culture is the gold standard in TB detection for its highest sensitivity, but requires weeks to obtain results. (cdc.gov)

Thus, intracellular bacteria frequently encode the capacity to regulate host lipogenesis as well as to modulate the lipid composition of host membranes. (microbialcell.com)

This allows for real-time monitoring of biological processes, such as gene expression, protein interactions, and cell migration, in a living organism. (rantdriven.com)
2014. Fringe proteins modulate Notch-ligand cis and trans interactions to specifysignaling states. (invivogen.com)

Microscopy analyses indicate that cholesterol regulates a step in L. pneumophila intracellular lifecycle that occurs after bacteria begin to replicate within an established intracellular niche. (microbialcell.com)

Luciferase is a luminescent Green Flourescent Protein reporter system that allows for the 24/7 tracking of hybrid humans (the vaxxed) through an external computer interface. (truthmed.social)
NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available - these attributes have made it popular with scientists since its release. (addgene.org)

It is used because bacteria may have resistance to some antibiotics. (wikipedia.org)
This resistance might be because a type of bacteria has intrinsic resistance to some antibiotics, because of resistance following past exposure to antibiotics, or because resistance may be transmitted from other sources such as plasmids. (wikipedia.org)

In a new take on use of luminescent nanoparticles as cancer imaging probes, a team of investigators from several research institutes in Paris, France, has developed new metal-containing nanoparticles that emit light for several hours after they are initially excited. (news-medical.net)

Chromoproteins are easy to detect reporter proteins. (springeropen.com)

As a tech-hungry world gobbles up rare-earth elements, researchers are adapting bacteria that can isolate and purify the metals in the absence of harsh chemicals. (nature.com)

The present invention relates generally to the field of molecular biology and protein engineering. (justia.com)
The scientists also managed to identify the molecular mechanism responsible involving an enzyme called chitinase and a chitin-binding protein. (thecbdtips.com)

The pertinent cis-trans isomerization and protonation state change of RFP chromophores in their local environments, involving key residues in protein matrices, lead to rich and complicated spectral features across multiple timescales. (bvsalud.org)
Taking advantage of the ability of chiral protein environments to induce optical activity, circular dichroism (CD) spectroscopy was employed to characterize the alkaloid-protein non-covalent adducts. (jascoinc.com)

This gene encodes a protein that is the master regulator of transformation and a homolog of Sxy, which was first described in Haemophilus influenzae [ 7 ],[ 8 ]. (biomedcentral.com)

Some make proteins that specifically interact with REEs, giving scientists the opportunity to isolate the elements from other metals, and perhaps even from each other. (nature.com)

In our current working model, the components of a type IV pilus combined with a few other competence proteins (such as ComEA, ComEC, and ComF) make up the majority of the DNA-uptake machinery. (biomedcentral.com)
Furthermore, we were able to show that the secondary cell type of the bacterium colonizes the fungal hyphae in particular. (thecbdtips.com)

Photoswitchable proteins play important roles during the development of new materials due to their capacity to change their conformational structure by providing/eliminating a specific light stimulus. (bvsalud.org)

A secondary cell form of the bacterium is responsible for this additional effect. (thecbdtips.com)

Angler fish grow luminescent bacteria in a special structure which dangles at the end of a stalk projecting from their forehead. (ucsd.edu)

This bacterium lives in symbiosis with small nematodes that penetrate the insect larvae and subsequently release the bacterium inside them. (thecbdtips.com)

Susceptibility testing methods are based on exposing bacteria to antibiotics and observing the effect on the growth of the bacteria (phenotypic testing), or identifying specific genetic markers (genetic testing). (wikipedia.org)
Previous studies in Haemophilus influenzae suggested that the CRP protein binds competence-specific CRP-S sites under competence-inducing conditions, most likely in concert with the master regulator of transformation Sxy/TfoX. (biomedcentral.com)

This environmental bacterium is found in the soil and water of tropical regions, such as Southeast Asia, where melioidosis is endemic. (cdc.gov)

Once a bacterium has been identified following microbiological culture, antibiotics are selected for susceptibility testing. (wikipedia.org)
Stab culture is not meant to be for frozen storage of bacteria. (riken.jp)

Watch as they dance and shimmer thanks to the presence of their specialized proteins and symbiotic bacteria that light up the ocean depths. (ubchomes.ca)
Even those animals not interested in eating dinoflagellates must be wary of their movements, because the ocean is like a luminescent minefield. (ucsd.edu)

Organisms21

Diseases1

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes25

Disciplines and Occupations2

Information Science4

Health Care6

Fluorescent10

Bacterial7

Fungi3

Bioluminescent bacteria1

Bioluminescence4

Genes1

Escherichia3

Cells9

Serum proteins1

Viruses1

Genetically modified2

Kinase2

Luminous1

Pathogen1

Antibodies2

Strains1

Coli5

Agar4

Firefly2

Strain1

Biomedical1

Fungus3

Nucleic acid1

Antibiotic6

Detection2

Lipid1

Interactions2

Intracellular1

Luciferase2

Resistance2

Nanoparticles1

Reporter1

Researchers1

Molecular2

Environments2

Gene1

Scientists1

Type2

Materials1

Cell1

Structure1

Lives1

Specific2

Found1

Culture2

Ocean2