• Certain antibodies can be used to detect or purify cells with these markers by binding to their surface antigens. (wikipedia.org)
  • ILC identified as CD45 + Thy1.2 + Lineage markers neg (CD3/CD4/CD5/CD8/CD11b/DX5/Gr-1/TCRδ/TCRβ/Ter-119 neg ). (elifesciences.org)
  • Although CD5+ macrophages were not present in the peritoneal cavities of normal mice, approximately 30% of the peritoneal macrophages in viable motheaten (mev/mev) mice, deficient in SHP-1 protein tyrosine phosphatase, expressed cell surface CD5 and B220, markers for B cells. (jax.org)
  • B1 cells express high levels of CD19, IgM and CD11b, and can be further divided into CD5 + B1a and CD5 - B1b subsets. (oncotarget.com)
  • The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. (thermofisher.com)
  • Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice. (jax.org)
  • Takahashi K, Miyakawa K, Wynn AA, Nakayama KI, Myint YY, Naito M, Shultz LD, Tominaga A, Takatsu K. Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice. (jax.org)
  • Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. (thermofisher.com)
  • B ) FACS analysis of CD4 neg CD8β neg skin T cells (gated on B220 - F4/80 - TCRβ + ) with control MR1/6-FP or MR1/5-OP-RU tetramer to identify MAIT cells in 5 mo mice. (elifesciences.org)
  • All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). (thermofisher.com)
  • In the peritoneal cavity of GM-CSF-treated mice, the percentages of hematopoietic progenitor cells doubly positive for CD5 and CD34 or c-kit and of macrophage precursor cells doubly positive for CD5 and ER-MP58 or ER-MP20 were increased significantly during the development of CD5+ macrophages and CD5 B cells, suggesting that CD5+ macrophages and B cells may share a bipotential progenitor in vivo. (jax.org)
  • We isolated fecal content and small intestinal leukocytes from murine, Roux-en-Y and end-to-end anastomoses, to identify microbiome changes and subsequent alterations in the regulatory and pro-inflammatory immune cells 3 days post-operative. (biomedcentral.com)
  • Chronic B cell activation may drive proliferation of antigen-selected B cell clones that accumulate genetic lesions and ultimately undergo neoplastic transformation. (biomedcentral.com)
  • Therefore, for example, the nonapoptotic vs. apoptotic cells in a heterogeneous sample can be rapidly compared for levels of BCL-2 and p53 protein expression, presence of a lineage-specific surface markers, such as T-cell specific antigen, and cell- cycle phase (G1/G0, S, or G2+M). (nih.gov)
  • They may be called naïve if they have not yet encountered their specific antigen. (biolegend.com)
  • The presence or absence of a specific antigen from the surface of the particular cell population is denoted with "+" or "-" respectively. (stratech.co.uk)
  • CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (thermofisher.com)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (thermofisher.com)
  • 3BP2(-/-) B cells demonstrated a proliferation defect in response to antigen receptor cross-linking and a heightened sensitivity to B-cell receptor-induced death via a caspase-3-dependent apoptotic pathway. (nih.gov)
  • For example, CD3 is a T cell co-receptor exclusively expressed on the surface of all mature T cells, while CD4 and CD8 antigens are expressed on the surface of T helper cells and cytotoxic T cells, respectively. (stratech.co.uk)
  • 6. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. (nih.gov)
  • By using appropriate standards and controls, flow cytometry can be exquisitely quantitative, allowing researchers to determine the exact number of molecules of fluorescent antibodies-and thus the molecules of antigen-bound to a cell or within a cell. (nih.gov)
  • 5. Efficiency of antigen presentation after antigen targeting to surface IgD, IgM, MHC, Fc gamma RII, and B220 molecules on murine splenic B cells. (nih.gov)
  • Here we show that mice lacking 3BP2 exhibited a perturbation in the peritoneal B1 and splenic marginal-zone B-cell compartments and diminished thymus-independent type 2 antigen response. (nih.gov)
  • In flow cytometric immunophenotyping, monoclonal antibodies targeting CD antigens are extensively used for the identification and characterization of leukocyte populations, subpopulations and differentiation stages. (stratech.co.uk)
  • Because CD antigens are expressed at varying degrees and cell types, multiparameter flow cytometric analysis utilizing two or more combinations of fluorescently labeled CD antibodies enables clear resolution of particular cell type or subtype. (stratech.co.uk)
  • This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • In addition, CD antigen expression can be influenced by certain disease conditions, and as such are often used as diagnostic or prognostic factors for the identification of leukemia's and lymphomas. (stratech.co.uk)
  • 20. Occupancy of CD72 (the CD5 counterstructure) enhances interleukin-4-dependent CD23 expression in resting B lymphocytes. (nih.gov)